scholarly journals Emergence of 16S rRNA Methylase Gene rmtB in Salmonella Enterica Serovar London and Evolution of RmtB-Producing Plasmid Mediated by IS26

2021 ◽  
Vol 11 ◽  
Author(s):  
Jing Wang ◽  
Zhen-Yu Wang ◽  
Yan Wang ◽  
Fan Sun ◽  
Wei Li ◽  
...  
Keyword(s):  
16S Rrna ◽  
Single Molecule ◽  
Resistance Genes ◽  
Variable Region ◽  
Mobile Elements ◽  
Plasmid Evolution ◽  
Complete Sequences ◽  
Methylase Gene ◽  
16S Rrna Methylase

This study aimed to characterize 16S rRNA methylase genes among Salmonella and to elucidate the structure and evolution of rmtB-carrying plasmids. One hundred fifty-eight Salmonella isolates from one pig slaughterhouse were detected as containing 16S rRNA methylase genes; two (1.27%) Salmonella London isolates from slaughtered pigs were identified to carry rmtB. They were resistant to gentamicin, amikacin, streptomycin, ampicillin, tetracycline, florfenicol, ciprofloxacin, and sulfamethoxazole/trimethoprim. The complete sequences of RmtB-producing isolates were obtained by PacBio single-molecule real-time sequencing. The isolate HA1-SP5 harbored plasmids pYUHAP5-1 and pYUHAP5-2. pYUHAP5-1 belonged to the IncFIBK plasmid and showed high similarity to multiple IncFIBK plasmids from Salmonella London in China. The rmtB-carrying plasmid pYUHAP5-2 contained a typical IncN-type backbone; the variable region comprising several resistance genes and an IncX1 plasmid segment was inserted in the resolvase gene resP and bounded by IS26. The sole plasmid in HA3-IN1 designated as pYUHAP1 was a cointegrate of plasmids from pYUHAP5-1-like and pYUHAP5-2-like, possibly mediated by IS26 via homologous recombination or conservative transposition. The structure differences between pYUHAP1 and its corresponding part of pYUHAP5-1 and pYUHAP5-2 may result from insertion, deletion, or recombination events mediated by mobile elements (IS26, ISCR1, and ISKpn43). This is the first report of rmtB in Salmonella London. IncN plasmids are efficient vectors for rmtB distribution and are capable of evolving by reorganization and cointegration. Our results further highlight the important role of mobile elements, particularly IS26, in the dissemination of resistance genes and plasmid evolution.

scholarly journals Characteristics of 32 Acinetobacter baumanni isolates co-harboring OXA-Carbapenemases and 16S rRNA methylase gene

2017 ◽  
Vol 11 (4) ◽  
pp. 110-116
Author(s):  
John Roberts Padde ◽  
Suleiman Hassan Jama ◽  
Li Xiaohan ◽  
Li Mingcheng
Keyword(s):  
16S Rrna ◽  
Methylase Gene ◽  
16S Rrna Methylase

Emergence of 16S rRNA methylase gene armA and cocarriage of blaIMP-1 in Pseudomonas aeruginosa isolates from South Korea

2010 ◽  
Vol 68 (4) ◽  
pp. 468-470 ◽  
Author(s):  
Mamata Gurung ◽  
Dong Chan Moon ◽  
Migma Dorji Tamang ◽  
Jungmin Kim ◽  
Yoo Chul Lee ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
South Korea ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Evolution and Comparative Genomics of F33:A−:B− Plasmids Carrying blaCTX-M-55 or blaCTX-M-65 in Escherichia coli and Klebsiella pneumoniae Isolated from Animals, Food Products, and Humans in China

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. e00137-18 ◽  
Author(s):  
Jing Wang ◽  
Zhen-Ling Zeng ◽  
Xin-Yi Huang ◽  
Zhen-Bao Ma ◽  
Ze-Wen Guo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Hot Spot ◽  
Antibiotic Resistance Genes ◽  
Mobile Elements ◽  
Evolutionary Potential ◽  
Variable Regions ◽  
Content Type ◽  
Complete Sequences

ABSTRACT To understand the underlying evolution process of F33:A−:B− plasmids among Enterobacteriaceae isolates of various origins in China, the complete sequences of 17 blaCTX-M-harboring F33:A−:B− plasmids obtained from Escherichia coli and Klebsiella pneumoniae isolates from different sources (animals, animal-derived food, and human clinics) in China were determined. F33:A−:B− plasmids shared similar plasmid backbones comprising replication, leading, and conjugative transfer regions and differed by the numbers of repeats in yddA and traD and by the presence of group II intron, except that pHNAH9 lacked a large segment of the leading and transfer regions. The variable regions of F33:A−B− plasmids were distinct and were inserted downstream of the addiction system pemI/pemK, identified as the integration hot spot among F33:A−B− plasmids. The variable region contained resistance genes and mobile elements or contained segments from other types of plasmids, such as IncI1, IncN1, and IncX1. Three plasmids encoding CTX-M-65 were very similar to our previously described pHN7A8 plasmid. Four CTX-M-55-producing plasmids contained multidrug resistance regions related to that of F2:A−B− plasmid pHK23a from Hong Kong. Five plasmids with IncN and/or IncX replication regions and IncI1-backbone fragments had variable regions related to those of pE80 and p42-2. The remaining five plasmids with IncN replicons and an IncI1 segment also possessed closely related variable regions. The diversity in variable regions was presumably associated with rearrangements, insertions, and/or deletions mediated by mobile elements, such as IS26 and IS1294. IMPORTANCE Worldwide spread of antibiotic resistance genes among Enterobacteriaceae isolates is of great concern. F33:A−:B− plasmids are important vectors of resistance genes, such as blaCTX-M-55/-65, blaNDM-1, fosA3, and rmtB, among E. coli isolates from various sources in China. We determined and compared the complete sequences of 17 F33:A−:B− plasmids from various sources. These plasmids appear to have evolved from the same ancestor by mobile element-mediated rearrangement, acquisition, and/or loss of resistance modules and similar IncN1, IncI1, and/or IncX1 plasmid backbone segments. Our findings highlight the evolutionary potential of F33:A−:B− plasmids as efficient vectors to capture and diffuse clinically relevant resistance genes.

scholarly journals Draft Genome Sequence of a Sequence Type 11 Klebsiella pneumoniae Clinical Strain Carrying a blaKPC-2 Carbapenemase Gene and an rmtB 16S rRNA Methylase Gene

2017 ◽  
Vol 5 (6) ◽  
Author(s):  
Zhihong Yao ◽  
Yu Feng ◽  
Li Wei ◽  
Zhiyong Zong
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Clinical Strain ◽  
Trna Genes ◽  
Carbapenemase Gene ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Klebsiella pneumoniae strain WCHKP649, recovered from a human wound, carried the carbapenemase gene bla KPC-2 and 16S rRNA methylase gene rmtB. Here, we report its 5.6-Mb draft genome sequence, comprising 171 contigs with an average 57.34% G+C content. The genome contained 5,284 coding sequences and 84 tRNA genes.

scholarly journals Emergence ofKlebsiella pneumoniaeCoproducing KPC-2 and 16S rRNA Methylase ArmA in Poland

2010 ◽  
Vol 55 (1) ◽  
pp. 443-446 ◽  
Author(s):  
Katarzyna Zacharczuk ◽  
Katarzyna Piekarska ◽  
Jolanta Szych ◽  
Elwira Zawidzka ◽  
Agnieszka Sulikowska ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Variable Region ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemic Strain ◽  
Pulsed Field ◽  
Ca 50 ◽  
16S Rrna Methylase

ABSTRACTAKlebsiella pneumoniaeepidemic strain that coproduced carbapenemase KPC-2 (K. pneumoniaecarbapenemase 2) and 16S rRNA methylase ArmA has emerged in Poland. Four nonduplicate isolates from patients in a hospital in Warsaw, Poland, were found to carry theblaKPC-2andarmAgenes on ca. 50-kb and 90-kb plasmids, respectively. Tn4401with a 100-bp deletion in the variable region was detected in all the isolates. XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of the isolates. All the isolates were resistant to carbapenems and 4,6-disubstituted 2-deoxystreptamines.

Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa

The Lancet ◽  
2003 ◽  
Vol 362 (9399) ◽  
pp. 1888-1893 ◽  
Author(s):  
Keiko Yokoyama ◽  
Yohei Doi ◽  
Kunikazu Yamane ◽  
Hiroshi Kurokawa ◽  
Naohiro Shibata ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Kayo Shimada ◽  
Shovita Shrestha ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Clinical Isolates ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following bla NDMs: bla NDM-1, bla NDM-3, bla NDM-4, bla NDM-5, bla NDM-7, bla NDM-12, and bla NDM-13. Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

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