scholarly journals Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

2021 ◽  
Vol 12 ◽  
Author(s):  
Rakesh Somashekaraiah ◽  
Walid Mottawea ◽  
Adithi Gunduraj ◽  
Udit Joshi ◽  
Riadh Hammami ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Fusarium Verticillioides ◽  
Antagonistic Activity ◽  
Fermented Food ◽  
Probiotic Properties ◽  
Type Iii Polyketide Synthase ◽  
Morphological Alterations ◽  
Biocontrol Potential ◽  
Traditional Fermented Food

The use of probiotics and antifungal capabilities of the lactic acid bacteria (LAB) isolated from different niches is a strategy to prepare functional cultures and biopreservatives for food/feed industries. In the present study, LAB strains isolated from an Indian traditional fermented food, Pozha, were evaluated for their probiotic properties and biocontrol potential. A total of 20 LAB isolates were selected from Pozha samples collected aseptically and screened for their antagonistic activity against Fusarium verticillioides. Among the bioactive isolates, Lacticaseibacillus brevis MYSN105 showed the highest antifungal activity in vitro, causing some morphological alterations such as damaged mycelia and deformed conidia. Cell-free supernatant (CFS) from L. brevis MYSN105 at 16% concentration effectively reduced the mycelial biomass to 0.369 g compared to 1.938 g in control. Likewise, the conidial germination was inhibited to 20.12%, and the seed treatment using CFS induced a reduction of spore count to 4.1 × 106 spores/ml compared to 1.1 × 109 spores/ml for untreated seeds. The internal transcribed spacer (ITS) copy number of F. verticillioides decreased to 5.73 × 107 and 9.026 × 107 by L. brevis MYSN105 and CFS treatment, respectively, compared to 8.94 × 1010 in control. The L. brevis MYSN105 showed high tolerance to in vitro gastrointestinal conditions and exhibited high adhesive abilities to intestinal epithelial cell lines. The comparative genome analysis demonstrated specific secondary metabolite region coding for bacteriocin and T3PKS (type III polyketide synthase) possibly related to survival and antimicrobial activity in the gut environment. Our results suggest that L. brevis MYSN105 has promising probiotic features and could be potentially used for developing biological control formulations to minimize F. verticillioides contamination and improve food safety measures.

scholarly journals Traditional Technologies and Probiotic Properties of Bacillus Strains Isolated from Kawal -A Chad Traditional Fermented Food Condiment

2019 ◽  
Vol 6 (2) ◽  
pp. 57-71 ◽  
Author(s):  
Abakar Idriss L ◽  
Abdelsalam Tidjani ◽  
Essodolom Taale ◽  
Abdoullahi Hissein O ◽  
Abel Tankoano ◽  
...  
Keyword(s):  
Fermented Food ◽  
Probiotic Properties ◽  
Traditional Technologies ◽  
Bacillus Strains ◽  
Traditional Fermented Food

scholarly journals Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation

2020 ◽  
Vol 8 (7) ◽  
pp. 1044
Author(s):  
Palaniselvam Kuppusamy ◽  
Dahye Kim ◽  
Ilavenil Soundharrajan ◽  
Hyung Soo Park ◽  
Jeong Sung Jung ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Lactic Acid Production ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Trifolium Incarnatum ◽  
Crimson Clover ◽  
Silage Fermentation ◽  
Low Carbohydrate

The objective of this study was to isolate and characterize lactic acid bacteria (LAB) with low carbohydrate tolerance from rumen fluid and to elucidate their probiotic properties and the quality of fermentation of Medicago sativa L. and Trifolium incarnatum L. silage in vitro. We isolated 39 LAB strains and screened for growth in MRS broth and a low-carbohydrate supplemented medium; among them, two strains, Lactiplantibacillus plantarum (Lactobacillus plantarum) RJ1 and Pediococcus pentosaceus S22, were able to grow faster in the low-carbohydrate medium. Both strains have promising probiotic characteristics including antagonistic activity against P. aeruginosa, E. coli, S. aureus, and E. faecalis; the ability to survive in simulated gastric-intestinal fluid; tolerance to bile salts; and proteolytic activity. Furthermore, an in vitro silage fermentation study revealed that alfalfa and crimson clover silage inoculated with RJ1 and S22 showed significantly decreased pH and an increased LAB population at the end of fermentation. Also, the highest lactic acid production was noted (p < 0.05) in LAB-inoculated silage vs. non-inoculated legume silage at high moisture. Overall, the data suggest that RJ1 and S22 could be effective strains for fermentation of legume silage.

scholarly journals Biosynthesis of Aliphatic Polyketides by Type III Polyketide Synthase and Methyltransferase in Bacillus subtilis

2009 ◽  
Vol 191 (15) ◽  
pp. 4916-4923 ◽  
Author(s):  
Chiaki Nakano ◽  
Hiroki Ozawa ◽  
Genki Akanuma ◽  
Nobutaka Funa ◽  
Sueharu Horinouchi
Keyword(s):  
Bacillus Subtilis ◽  
Polyketide Synthase ◽  
Bacterial Genome ◽  
Fatty Acyl ◽  
Gram Positive ◽  
Type Iii Polyketide Synthase ◽  
Type Iii ◽  
Vitro Analysis

ABSTRACT Type III polyketide synthases (PKSs) synthesize a variety of aromatic polyketides in plants, fungi, and bacteria. The bacterial genome projects predicted that probable type III PKS genes are distributed in a wide variety of gram-positive and -negative bacteria. The gram-positive model microorganism Bacillus subtilis contained the bcsA-ypbQ operon, which appeared to encode a type III PKS and a methyltransferase, respectively. Here, we report the characterization of bcsA (renamed bpsA, for Bacillus pyrone synthase, on the basis of its function) and ypbQ, which are involved in the biosynthesis of aliphatic polyketides. In vivo analysis demonstrated that BpsA was a type III PKS catalyzing the synthesis of triketide pyrones from long-chain fatty acyl-coenzyme A (CoA) thioesters as starter substrates and malonyl-CoA as an extender substrate, and YpbQ was a methyltransferase acting on the triketide pyrones to yield alkylpyrone methyl ethers. YpbQ thus was named BpsB because of its functional relatedness to BpsA. In vitro analysis with histidine-tagged BpsA revealed that it used broad starter substrates and produced not only triketide pyrones but also tetraketide pyrones and alkylresorcinols. Although the aliphatic polyketides were expected to localize in the membrane and play some role in modulating the rigidity and properties of the membrane, no detectable phenotypic changes were observed for a B. subtilis mutant containing a whole deletion of the bpsA-bpsB operon.

scholarly journals Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

2017 ◽  
Vol 57 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Zeinab Fotoohiyan ◽  
Saeed Rezaee ◽  
Gholam Hosein Shahidi Bonjar ◽  
Amir Hossein Mohammadi ◽  
Mohammad Moradi
Keyword(s):  
Verticillium Dahliae ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

scholarly journals Isolation, identification and potential probiotic characterization of lactic acid bacteria from Thai traditional fermented food

2021 ◽  
Vol 7 (4) ◽  
pp. 431-446
Author(s):  
Sunisa Suwannaphan ◽  
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
High Surface ◽  
Surface Hydrophobicity ◽  
Fermented Food ◽  
Probiotic Properties ◽  
Two Samples ◽  
Traditional Fermented Food

<abstract> <p>The probiotic potential of lactic acid bacteria (LAB) isolated from Thai traditional fermented food was investigated. Forty-two samples were collected from four markets in Phra Nakhon Si Ayutthaya Province. Out of 50 isolated LAB, 6 (a3, f4, f8, K1, K4 and K9) obtained from pla-ra and bamboo shoot pickle samples showed high tolerance to gastrointestinal tract conditions. These isolates were selected to identify and characterize their probiotic properties. Isolate a3 was identified as <italic>Weissella thailandensis</italic>, isolates f4 and f8 were identified as belonging to <italic>Enterococcus thailandicus</italic> and isolates K1, K4 and K9 were determined as <italic>Limosilactobacillus fermentum</italic>. All six LAB exhibited high autoaggregation ability (93.40–95.01%), while <italic>W. thailandensis</italic> isolate a3 showed potential for coaggregation in almost all the pathogenic bacteria tested. Cell-free supernatant (CFS) obtained from all isolates did not inhibit <italic>Staphylococcus aureus</italic>. CFS derived from <italic>L. fermentum</italic> isolate K4 showed the most efficient antimicrobial activity, in particular against Gram-negative bacteria, while <italic>L. fermentum</italic> isolate K4 presented high surface hydrophobicity in the presence of xylene and n-hexane. All LAB isolates were found to be resistant to clindamycin and nalidixic acid, whereas <italic>E. thailandicus</italic> isolate f8 exhibited resistance to most of the antibiotics tested. <italic>L. fermentum</italic> isolate K4 showed promise as a suitable probiotic candidate for future applications in the food industry due to tolerance to gastrointestinal tract conditions with high surface hydrophobicity and inhibited most of the pathogens tested.</p> </abstract>

scholarly journals Potential probiotic yeast isolated from an Indonesian indigenous fermented fish (Ikan Budu)

10.5219/1544 ◽  
2021 ◽  
Vol 15 ◽  
pp. 460-466
Author(s):  
Yetti Marlida ◽  
Nurul Huda ◽  
Harnentis ◽  
Yuliaty Shafan Nur ◽  
Nuri Mekar Lestari ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Bile Acid ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Acid Tolerance ◽  
Fermented Food ◽  
Probiotic Properties

Budu is a fermented food resulting from the activities of microorganisms like lactic acid bacteria and yeast. Budu, therefore, serves as a source of probiotics that can have beneficial effects on livestock and humans. Nonetheless, their selection has to be done with caution. The current study purposed to find out whether budu has desirable probiotic properties. This was done by determining its pH, bile acid tolerance, hydrophobicity, and inhibition of pathogens such as Staphylococcus aureus, Salmonella enteritidis, and Escherichia coli. An in vitro experiment was conducted using three Saccharomyces cerevisiae (coded as SC 11, SC 12, and SC 21) in the preparation of budu. The whole experiment was repeated four times. The budus were tested for their probiotic properties (low pH, bile salts, hydrophobicity, and inhibition of pathogenic bacteria). The results showed that the three Saccharomyces cerevisiae survived in gastric juice and bile acid, exhibited good hydrophobicity, and could inhibit pathogenic bacteria, both gram-positive and negative pathogens. They were able to survive at pH 2 for 3 h (40.70 to 55.1%), at pH 2 for 5 h (35.25 to 46.88%), in 0.3% bile acid incubated for 3 h (69.69 to 86.56%), in 0.3% bile acid incubated for 5 h (82.22 to 88.18%) and hydrophobicity ability of 97.0 to 98.1%. The inhibition activity against pathogenic bacteria, that is, Escherichia coli was 2.50 to 3.81 mm, Staphylococcus aureus was 1.66 to 3.71 mm, and Salmonella enteritidis was 1.20 to 2.64 mm.

scholarly journals Influence of Dehydrated Wheat/Rice Cereal Matrices on Probiotic Activity of Bifidobacterium animalis ssp. lactis BB-12®

2019 ◽  
Vol 57 (2) ◽  
pp. 147-158 ◽  
Author(s):  
Andreja Leboš Pavunc ◽  
Jasna Novak ◽  
Jagoda Šušković ◽  
Blaženka Kos ◽  
Ksenija Durgo ◽  
...  
Keyword(s):  
Genome Stability ◽  
Storage Period ◽  
Probiotic Strain ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Intestinal Epithelial ◽  
The Novel ◽  
Bifidobacterium Animalis ◽  
Food Matrices

Three novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12® (BB-12®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12®.

In vitro evaluation by PCA and AHP of potential antidiabetic properties of lactic acid bacteria isolated from traditional fermented food

LWT ◽  
2019 ◽  
Vol 115 ◽  
pp. 108455 ◽  
Author(s):  
Ting Cai ◽  
Haizhen Wu ◽  
Jialiang Qin ◽  
Jinnan Qiao ◽  
Yuanxin Yang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fermented Food ◽  
In Vitro Evaluation ◽  
Traditional Fermented Food

scholarly journals Selection criteria of Pediococcus sp. and Bifidobacterium bifidum Strains Isolated from Cereals Based on Fermented Food for Probiotic Use

2020 ◽  
Vol 2 (1) ◽  
pp. 71
Keyword(s):  
Selection Criteria ◽  
Antagonistic Activity ◽  
Bifidobacterium Bifidum ◽  
Fermented Food ◽  
In Vitro Tests ◽  
Fermented Foods ◽  
Good Adherence ◽  
Ph Values ◽  
Bile Resistance

Cereal based Fermented foods are a rich source of Probiotic organisms. Once if the Probiotic organisms are consumed, it should be capable of adhering to the colon region. To find out the efficiency of Pediococcus sp. and Bifidobacterium sp. they were subjected to in-vitro tests such as tolerance to bile, resistance to low pH values, cell adhesion and antagonistic activity among the two organisms, Bifidobacterium sp. showed better growth at pH 3.5 than Pediococcus sp. during the increased incubation time. Cells remain viable up to 0.8% of bile concentration beyond which the multiplication was slow in the case of both the strains. Bifidobacterium sp. showed good adherence to the substratum than Pediococcus sp. Both the organisms were antagonistic against common enteric pathogens, which is an ideal characteristic of a probiotic.

Sign in / Sign up

Export Citation Format

Share Document