scholarly journals Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation

2020 ◽  
Vol 8 (7) ◽  
pp. 1044
Author(s):  
Palaniselvam Kuppusamy ◽  
Dahye Kim ◽  
Ilavenil Soundharrajan ◽  
Hyung Soo Park ◽  
Jeong Sung Jung ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Lactic Acid Production ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Trifolium Incarnatum ◽  
Crimson Clover ◽  
Silage Fermentation ◽  
Low Carbohydrate

The objective of this study was to isolate and characterize lactic acid bacteria (LAB) with low carbohydrate tolerance from rumen fluid and to elucidate their probiotic properties and the quality of fermentation of Medicago sativa L. and Trifolium incarnatum L. silage in vitro. We isolated 39 LAB strains and screened for growth in MRS broth and a low-carbohydrate supplemented medium; among them, two strains, Lactiplantibacillus plantarum (Lactobacillus plantarum) RJ1 and Pediococcus pentosaceus S22, were able to grow faster in the low-carbohydrate medium. Both strains have promising probiotic characteristics including antagonistic activity against P. aeruginosa, E. coli, S. aureus, and E. faecalis; the ability to survive in simulated gastric-intestinal fluid; tolerance to bile salts; and proteolytic activity. Furthermore, an in vitro silage fermentation study revealed that alfalfa and crimson clover silage inoculated with RJ1 and S22 showed significantly decreased pH and an increased LAB population at the end of fermentation. Also, the highest lactic acid production was noted (p < 0.05) in LAB-inoculated silage vs. non-inoculated legume silage at high moisture. Overall, the data suggest that RJ1 and S22 could be effective strains for fermentation of legume silage.

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

scholarly journals GLUCOSE CONSUMPTION AND LACTIC ACID PRODUCTION BY ERYTHROCYTES FROM DOUBLE-MUSCLED CATTLE

1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON
Keyword(s):  
Lactic Acid ◽  
Key Words ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Age Groups ◽  
Phenotypic Expression ◽  
Glucose Consumption ◽  
Molar Ratio ◽  
Breed Group

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid

scholarly journals Lactic Acid Bacteria Co-Encapsulated with Lactobionic Acid: Probiotic Viability during In Vitro Digestion

2021 ◽  
Vol 11 (23) ◽  
pp. 11404
Author(s):  
Sara Sáez-Orviz ◽  
Francesca Passannanti ◽  
Marianna Gallo ◽  
Rosa Colucci Cante ◽  
Federica Nigro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Saline Solution ◽  
Lactic Acid Production ◽  
In Vitro Digestion ◽  
Lactobionic Acid ◽  
Different Types ◽  
Stability Time ◽  
Over Time ◽  
Improve Health

Synbiotic products are a type of functional food with great potential due to consumer interest in foods that improve health and/or reduce the risk of certain diseases. In this study, synbiotic macrocapsules were developed using Lactobacillus paracasei CBA L74 as probiotic and lactobionic acid (LBA) as prebiotic. Firstly, the probiotic was proven to be able to use LBA as the only substrate source checking their growth and lactic acid production. Then, four different types of capsules were produced using sodium alginate as matrix and different hardener solutions (CaCl2 and chitosan). The macrocapsules were characterised regarding their strength, and the best performing ones were used for further analysis. In order to obtain a synbiotic capsule characterized by a longer stability time due to low water activity, the capsules were dried using freeze and thermal drying. Successively, to revitalize the microorganisms, the capsules were rehydrated in two different media (saline solution and yogurt), subjected to simulated in vitro digestion tests and visually characterised. In addition, their viability over time was assessed. As a result, L. paracasei was able to grow using LBA as the only source of carbon with better production of lactic acid for prolonged periods. The more resistant freeze- and thermal-dried capsules showed differences in rehydration kinetics, and visual changes were also observed. In simulated in vitro digestion tests, capsules rehydrated in yogurt showed the best results in terms of survival. Regarding their viability over time, the importance of the use of chitosan was noted.

Carbohydrate Metabolism of Chick Fibroblasts in Vitro

1942 ◽  
Vol 18 (3) ◽  
pp. 237-256
Author(s):  
E. N. WILLMER
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Acid Production ◽  
Lactic Acid Production ◽  
High Growth ◽  
Glucose Consumption ◽  
High Growth Rate ◽  
Absolute Correlation ◽  
Heart Cultures

1. The glucose consumption of osteoblasts growing in a medium of plasma and embryo juice has been compared with that of the same cells in plasma and Tyrode medium. Embryo juice causes an initial increased glucose consumption which later falls below the level characteristic of a Tyrode-plasma medium. 2. A modification of a method of measuring the nucleoprotein phosphorus content of tissue cultures is described and among other data obtained the changes which occur in this figure have been noted for fourth passage chick osteoblast cultures and for fresh heart explants during the first 4 days of culture in media of plasma and Tyrode and of plasma and embryo juice. 3. The figures for nucleoprotein content have been correlated with those for glucose consumption and lactic acid production and pronounced differences have been observed in the behaviour of the two types of tissue. The possible significance of the findings is discussed. 4. There is no absolute correlation between high growth rate and high sugar consumption or lactic acid production. 5. The data discussed are consistent with the idea that an increased protein metabolism, and a decreased carbohydrate metabolism result from the addition of embryo juice to osteoblast cultures. Embryo juice has no detectable effects on the carbohydrate metabolism of fresh heart cultures.

scholarly journals In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guesh Mulaw ◽  
Tesfaye Sisay Tessema ◽  
Diriba Muleta ◽  
Anteneh Tesfaye
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Lactobacillus Paracasei ◽  
Probiotic Properties ◽  
Sequence Comparisons

Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35–97.11% and 38.40–90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to be potentially useful to produce probiotic products.

Stimulation of Lactic Acid Production in Vitro from Prepubertal Rat Ovaries by Gonadotropins and Their Subunits1

Endocrinology ◽  
1973 ◽  
Vol 92 (4) ◽  
pp. 1022-1027 ◽  
Author(s):  
SUSAN WALKER FARMER ◽  
M. R. SAIRAM ◽  
HAROLD PAPKOFF
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Stimulation Of

scholarly journals Respiration and Glycolysis of Normal Human Lymphocytes

Blood ◽  
1966 ◽  
Vol 28 (2) ◽  
pp. 163-174 ◽  
Author(s):  
CARL J. HEDESKOV ◽  
VIGGO ESMANN
Keyword(s):  
Oxygen Consumption ◽  
Lactic Acid ◽  
Acid Production ◽  
Glucose Utilization ◽  
Aerobic Glycolysis ◽  
Human Lymphocytes ◽  
Lactic Acid Production ◽  
Crowding Effect ◽  
Normal Human

Abstract The metabolism of intact, normal, human lymphocytes in vitro was studied from a total of 80 subjects. Corrected for the metabolism of contaminating red blood cells, the glucose uptake, lactic acid production, and oxygen consumption were 62, 95, and 117 µmoles per 1010 lymphocytes per hour, respectively, provided the cells were incubated at concentrations greater than 40 x 106 lymphocytes per ml. At lower lymphocyte concentrations the oxygen consumption per lymphocyte rose steeply with decreasing cell concentration (crowding effect). A similar but weaker crowding effect was noted for the lactic acid production, but not for the utilization of glucose. The oxygen uptake was lower with 20 per cent than with 100 per cent oxygen as gas phase. Small Pasteur and Crabtree effects were demonstrated. The oxygen consumption and lactic acid production proceeded linear with time, while the glucose utilization was higher during the first 30 minutes of incubation than later on. It is concluded that lymphocytes have a low aerobic glycolysis accounting for 75 per cent of the glucose utilization. The respiration is severely inhibited at high cell concentrations and it is suggested that this is caused by an insufficient availability of oxygen to the cells.

Sign in / Sign up

Export Citation Format

Share Document