Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis

In our previous study, it was shown that Riemerella anatipestifer, a Gram-negative bacterium, is naturally competent, but the genes involved in the process of natural transformation remain largely unknown. In this study, a random transposon mutant library was constructed using the R. anatipestifer ATCC11845 strain to screen for the genes involved in natural transformation. Among the 3000 insertion mutants, nine mutants had completely lost the ability of natural transformation, and 14 mutants showed a significant decrease in natural transformation frequency. We found that the genes RA0C_RS04920, RA0C_RS04915, RA0C_RS02645, RA0C_RS04895, RA0C_RS05130, RA0C_RS05105, RA0C_RS09020, and RA0C_RS04870 are essential for the occurrence of natural transformation in R. anatipestifer ATCC11845. In particular, RA0C_RS04895, RA0C_RS05130, RA0C_RS05105, and RA0C_RS04870 were putatively annotated as ComEC, DprA, ComF, and RecA proteins, respectively, in the NCBI database. However, RA0C_RS02645, RA0C_RS04920, RA0C_RS04915, and RA0C_RS09020 were annotated as proteins with unknown function, with no homology to any well-characterized natural transformation machinery proteins. The homologs of these proteins are mainly distributed in the members of Flavobacteriaceae. Taken together, our results suggest that R. anatipestifer encodes a unique natural transformation machinery.

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Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer

Frontiers in Microbiology ◽

10.3389/fmicb.2021.644868 ◽

2021 ◽

Vol 12 ◽

Author(s):

Li Zhang ◽

Li Huang ◽

Mi Huang ◽

Mengying Wang ◽

Dekang Zhu ◽

...

Keyword(s):

Natural Transformation ◽

Iron Acquisition ◽

Vitamin B1 ◽

Underlying Mechanism ◽

Iron Chelator ◽

Transformation Frequency ◽

Nutritional Factors ◽

Riemerella Anatipestifer ◽

The Family ◽

Competent Bacterium

Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain unknown. In this study, we evaluated the effects of various nutritional factors of the GCB medium [glucose, L-glutamine, vitamin B1, Fe (NO3)3, NaCl, phosphate, and peptone], on the natural transformation of R. anatipestifer ATCC 11845. Among the assayed nutrients, peptone and phosphate affected the natural transformation of R. anatipestifer ATCC 11845, and the transformation frequency was significantly decreased when phosphate or peptone was removed from the GCB medium. When the iron chelator 2,2′-dipyridyl (Dip) was added, the transformation frequency was decreased by approximately 100-fold and restored gradually when Fe (NO3)3 was added, suggesting that the natural transformation of R. anatipestifer ATCC 11845 requires iron. Given the importance of TonB in nutrient transportation, we further identified whether TonB is involved in the natural transformation of R. anatipestifer ATCC 11845. Mutation of tonBA or tonBB, but not tbfA, was shown to inhibit the natural transformation of R. anatipestifer ATCC 11845 in the GCB medium. In parallel, it was shown that the tonBB mutant, but not the tonBA mutant, decreased iron acquisition in the GCB medium. This result suggested that the tonBB mutant affects the natural transformation frequency due to the deficiency of iron utilization.

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Draft Genome Sequence of Riemerella anatipestifer Isolate 17CS0503

Genome Announcements ◽

10.1128/genomea.00274-18 ◽

2018 ◽

Vol 6 (20) ◽

Author(s):

Anne Busch ◽

Martin Ryll ◽

Alexander Immel ◽

Sabin Kornell ◽

Ben Krause-Kyora ◽

...

Keyword(s):

Genome Sequence ◽

Anas Platyrhynchos ◽

Draft Genome ◽

Draft Genome Sequence ◽

Negative Bacterium ◽

Gram Negative ◽

Content Type ◽

Riemerella Anatipestifer ◽

Peking Duck ◽

The Family

ABSTRACT Riemerella anatipestifer is a Gram-negative bacterium belonging to the family Flavobacteriaceae. It is primarily associated with acute septicemia in younger birds. The R. anatipestifer isolate 17CS0503 described here was isolated from a Peking duck (Anas platyrhynchos domesticus) in Hannover, Germany, in 1999.

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Isolation of d-Talomethylose (6-Deoxy-d-talose) and d-Rhamnose (6-Deoxy-d-mannose) from Capsular Polysaccharide of a Gram-negative Bacterium

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)73934-9 ◽

1962 ◽

Vol 237 (6) ◽

pp. 1767-1771

Author(s):

Alvin Markovitz

Keyword(s):

Capsular Polysaccharide ◽

Negative Bacterium ◽

Gram Negative

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Massiliamide, a cyclic tetrapeptide with potent tyrosinase inhibitory properties from the Gram-negative bacterium Massilia albidiflava DSM 17472T

The Journal of Antibiotics ◽

10.1038/s41429-020-00394-y ◽

2020 ◽

Author(s):

Andri Frediansyah ◽

Jan Straetener ◽

Heike Brötz-Oesterhelt ◽

Harald Gross

Keyword(s):

Absolute Configuration ◽

Inhibitory Activity ◽

Liquid Culture ◽

Spectroscopic Analysis ◽

2D Nmr ◽

Negative Bacterium ◽

Gram Negative ◽

The Absolute ◽

Potent Inhibitory Activity ◽

Cyclic Tetrapeptide

AbstractA cyclic tetrapeptide, designated massiliamide, was isolated from the liquid culture of the Gram-negative bacterium Massilia albidiflava DSM 17472T. The structure was elucidated through extensive spectroscopic analysis, including HR-MS and 1D and 2D NMR experiments. The absolute configuration was determined using the Marfey´s method. Massiliamide showed potent inhibitory activity towards tyrosinase with an IC50 value of 1.15 µM and no cytotoxicity.

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Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide

Communications Biology ◽

10.1038/s42003-020-01621-w ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Thomas V. Heidler ◽

Karin Ernits ◽

Agnieszka Ziolkowska ◽

Rolf Claesson ◽

Karina Persson

Keyword(s):

Porphyromonas Gingivalis ◽

Von Willebrand Factor ◽

Host Cells ◽

Negative Bacterium ◽

Oral Biofilm ◽

Gram Negative ◽

Type V ◽

Von Willebrand ◽

Willebrand Factor ◽

Fimbrial Protein

AbstractThe Gram-negative bacterium Porphyromonas gingivalis is a secondary colonizer of the oral biofilm and is involved in the onset and progression of periodontitis. Its fimbriae, of type-V, are important for attachment to other microorganisms in the biofilm and for adhesion to host cells. The fimbriae are assembled from five proteins encoded by the mfa1 operon, of which Mfa5 is one of the ancillary tip proteins. Here we report the X-ray structure of the N-terminal half of Mfa5, which reveals a von Willebrand factor domain and two IgG-like domains. One of the IgG-like domains is stabilized by an intramolecular isopeptide bond, which is the first such bond observed in a Gram-negative bacterium. These features make Mfa5 structurally more related to streptococcal adhesins than to the other P. gingivalis Mfa proteins. The structure reported here indicates that horizontal gene transfer has occurred among the bacteria within the oral biofilm.

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Anion inhibition studies of the Zn(II)-bound ι-carbonic anhydrase from the Gram-negative bacterium Burkholderia territorii

Journal of Enzyme Inhibition and Medicinal Chemistry ◽

10.1080/14756366.2020.1867122 ◽

2021 ◽

Vol 36 (1) ◽

pp. 372-376

Author(s):

Andrea Petreni ◽

Viviana De Luca ◽

Andrea Scaloni ◽

Alessio Nocentini ◽

Clemente Capasso ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Negative Bacterium ◽

Gram Negative ◽

Inhibition Studies

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The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species

Carbohydrate Research ◽

10.1016/j.carres.2020.108135 ◽

2020 ◽

Vol 497 ◽

pp. 108135

Author(s):

Agnieszka Kowalczyk ◽

Nikola Szpakowska ◽

Wojciech Sledz ◽

Agata Motyka-Pomagruk ◽

Karolina Ossowska ◽

...

Keyword(s):

Negative Bacterium ◽

Gram Negative

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Draft Genome Sequence of Pantoea sp. Strain A4, a Rafflesia-Associated Bacterium That ProducesN-Acylhomoserine Lactones as Quorum-Sensing Molecules

Journal of Bacteriology ◽

10.1128/jb.01619-12 ◽

2012 ◽

Vol 194 (23) ◽

pp. 6610-6610 ◽

Cited By ~ 12

Author(s):

Kar-Wai Hong ◽

Han Ming Gan ◽

Siew-Moon Low ◽

Patrick Kok Yuen Lee ◽

Yee-Meng Chong ◽

...

Keyword(s):

Quorum Sensing ◽

Genome Sequence ◽

Draft Genome ◽

Draft Genome Sequence ◽

Negative Bacterium ◽

Gram Negative ◽

Content Type ◽

First Time

ABSTRACTPantoeasp. strain A4 is a Gram-negative bacterium isolated from theRafflesiaflower. We present here, for the first time, the genome sequence ofRafflesia-associatedPantoeasp. strain A4, which exhibited quorum-sensing activity.

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Transposition of Tn5367 in Mycobacterium marinum, Using a Conditionally Recombinant Mycobacteriophage

Journal of Bacteriology ◽

10.1128/jb.185.5.1745-1748.2003 ◽

2003 ◽

Vol 185 (5) ◽

pp. 1745-1748 ◽

Cited By ~ 9

Author(s):

Jan Rybniker ◽

Martina Wolke ◽

Christiane Haefs ◽

Georg Plum

Keyword(s):

Mycobacterium Tuberculosis ◽

Skin Infection ◽

Model Organism ◽

Transposon Mutagenesis ◽

Mycobacterium Marinum ◽

Close Relative ◽

Mutant Library ◽

Human Pathogen ◽

Valid Model ◽

Highly Efficient

ABSTRACT Mycobacterium marinum is a close relative of the obligate human pathogen Mycobacterium tuberculosis. As with M. tuberculosis, M. marinum causes intracellular infection of poikilothermic vertebrates and skin infection in humans. It is considered a valid model organism for the study of intracellular pathogenesis of mycobacteria. Low transformation efficiencies for this species have precluded approaches using mutant libraries in pathogenesis studies. We have adapted the conditionally replicating mycobacteriophage phAE94, originally developed as a transposon mutagenesis tool for M. tuberculosis, to meet the specific requirements of M. marinum. Conditions permissive for phage replication in M. tuberculosis facilitated highly efficient transposon delivery in M. marinum. Using this technique we succeeded in generating a representative mutant library of this species, and we conclude that TM4-derived mycobacteriophages are temperature-independent suicide vectors for M. marinum.

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