Efficacy of crude extracts of Clitoria ternatea for antibacterial activity against gram negative bacterium (Proteus mirabilis)

Citrus peel is an important source of essential oils (EOs). However, these EOs are not invested, although the annual production of citrus is high in Syria. The current study aimed to investigate chemical composition and antibacterial activity of some citrus peel EOs, namely: lemon (Citrus limon), orange (C. sinensis), grapefruit (C. paradisi), mandarin (C. reticulata) and bitter orange (C. aurantium). Gas chromatography–mass spectrometry (GC/MS) (gas chromatograph type: Agilent 7890A, auto sampler type: Agilent 7683B coupled to mass spectrometer, type Agilent 5975C, using DB–1 capillary column. EOs. concentration 1: 10 v/v in chloroform, injection volume 1 µl, split ratio 1: 80), was used to identify the chemical composition of the EOs, which were extracted by hydrodistillation technique, and chemical composition was expressed as Mean ± SD of three replications using SPSS V17 software. Minimal bactericidal concentration (MBC) was used to determine the antibacterial activity against five Gram-positive bacteria (Bacillus cereus, B. licheniformis, Staphylococcus haemolyticus, S. lugdunensis, Enterococcus faecalis) and five Gram negative bacteria (Klebsiella oxytoca, Citrobacter koseri, Serratia liquefaciens, Pseudomonas fluorescens and P. luteola). Limonene formed the vast majority of EOs (between 62.16 and 95.26% in lemon and orange EOs, respectively), but there were other active components, such as α–Pinene and β–Pinene. Lemon EO was the most effective one, with MBC values ranged between 4 μl.ml–1 (against Bacillus cereus) and 50 μl.ml–1 (against Serratia liquefaciens). Pseudomonas luteola (a Gram-negative bacterium) was the most sensitive species to citrus EOs (MBC values ranged between 4 and 50 μl.ml–1 for lemon and orange EOs, respectively); while S. liquefaciens (a Gram-negative bacterium) was the most resistant bacterium (MBC values were 50 and 150 μl.ml–1 for lemon and mandarin EOs, respectively) among all species studied in the current research.

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Synthesis and Antibacterial Activity of Novel Schiff Bases of Thiosemicarbazone Derivatives With Adamantane Moiety

10.21203/rs.3.rs-407353/v1 ◽

2021 ◽

Author(s):

Jiahui Zhu ◽

Guosheng Teng ◽

Dongfeng Li ◽

Ruibin Hou ◽

Yan Xia

Keyword(s):

Antibacterial Activity ◽

Antibacterial Agents ◽

Bacterial Resistance ◽

Inhibitory Effect ◽

Negative Bacterium ◽

Gram Negative ◽

Major Threat ◽

Antibiotic Drugs

Abstract Increased bacterial resistance to antibiotics is a major threat to human health, and it is particularly important to develop novel antibiotic drugs. Here, we designed a series of Schiff base thiosemicarbazone derivatives containing an adamantane moiety, and carried out structural characterization of the compounds and in vitro antibacterial activity tests. Compound 7e was as effective as the commonly-used antibiotic ampicillin against the Gram-negative bacterium Escherichia coli, and compound 7g had a good inhibitory effect against Gram-positive Bacillus subtilis. These findings provide data for the development of better thiosemicarbazone antibacterial agents.

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Antimicrobial Activity of Extracts and Fractions from Aerial Parts of Selected Plants (Garcinia achachairu, Macrosiphonia velame, Rubus niveus and Pilea microphylla) Against Some Pathogenic Microorganisms

Natural Product Communications ◽

10.1177/1934578x1300801117 ◽

2013 ◽

Vol 8 (11) ◽

pp. 1934578X1300801 ◽

Cited By ~ 1

Author(s):

Carla Melim ◽

Karoliny Guimarães ◽

Zhelmy Martin-Quintal ◽

Aurea Damaceno Alves ◽

Domingos Tabajara de Oliveira Martins ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Pathogenic Microorganisms ◽

Negative Bacterium ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Brazilian Flora ◽

Brazilian Biodiversity

As part of the program of our research group to search for new and effective substances from the Brazilian biodiversity, the present work evaluates the antibacterial activity of four species from the Brazilian flora ( Garcinia achachairu, Macrosiphonia velame, Rubus niveus and Pilea microphylla) against Bacillus subtilis, Staphylococcus aureus and S. saprophyticus (Gram-positive bacteria), Escherichia coli (Gram-negative bacterium) and Candida albicans (yeast). The extracts of R. niveus and M. velame showed promising antibacterial activity with MICs, ranging from 1000 to 125 μg/mL. Bio-guided fractionation of M. velame yielded four compounds, with the highest inhibition being observed for compound 3, with a MIC of 125 μg/mL against S. aureus. The combinations of fractions 2 and 4 showed beneficial effect against Gram-positive bacteria (additive effect), suggesting a possible synergistic effect.

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Complete Genome Sequence of Proteus mirabilis Siphophage Stubb

Microbiology Resource Announcements ◽

10.1128/mra.01185-19 ◽

2019 ◽

Vol 8 (43) ◽

Author(s):

Taylor A. Bourgeois ◽

Lauren Lessor ◽

Chandler O’Leary ◽

Rohit Kongari ◽

Mei Liu

Keyword(s):

Genome Sequence ◽

Proteus Mirabilis ◽

Complete Genome Sequence ◽

Complete Genome ◽

Negative Bacterium ◽

Common Cause ◽

Gram Negative ◽

Content Type ◽

The Family ◽

Tract Infections

Proteus mirabilis, a Gram-negative bacterium belonging to the family Enterobacteriaceae, is a common cause of urinary tract infections. Phages infecting Proteus mirabilis could be used as therapeutics to treat infections caused by this bacterium. This announcement describes the complete genome sequence of the T5-like P. mirabilis phage Stubb.

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Draft Genome Sequence of Proteus mirabilis UMB1310, Isolated from the Female Urinary Tract

Microbiology Resource Announcements ◽

10.1128/mra.00390-20 ◽

2020 ◽

Vol 9 (20) ◽

Author(s):

Olivia Allen ◽

Taylor Miller-Ensminger ◽

Adelina Voukadinova ◽

Alan J. Wolfe ◽

Catherine Putonti

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Genome Sequence ◽

Proteus Mirabilis ◽

Draft Genome ◽

Negative Bacterium ◽

Gram Negative ◽

Content Type ◽

Female Urinary Tract

Proteus mirabilis is a Gram-negative bacterium that is linked to common complications within the urinary tract. Here, we present the draft genome for P. mirabilis UMB1310, which was isolated from the urine of a woman with a urinary tract infection.

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ZnO Nanoparticles-Chitosan Composite as Antibacterial Finish for Textiles

International Journal of Carbohydrate Chemistry ◽

10.1155/2012/693629 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 16

Author(s):

Asmaa Farouk ◽

Shaaban Moussa ◽

Mathias Ulbricht ◽

Torsten Textor

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Zno Nanoparticles ◽

Micrococcus Luteus ◽

Sol Gel ◽

Negative Bacterium ◽

Hybrid Polymers ◽

Gram Negative ◽

Organic Hybrid ◽

Equal Degree

The antibacterial performance of sol-gel-derived inorganic-organic hybrid polymers filled with ZnO nanoparticles-chitosan against a gram-negative bacterium Escherichia coli and a gram-positive Micrococcus luteus has been investigated. Three different molecular weights (MW) of chitosan (CTS) 1.36 · 105, 2.2 · 105, and 3.0 · 105 Da with equal degree of deacetylation (DD, 85%) (coded as S 85-60, He 85-250, and He 85-500) with equal degree of deacetylation (DD, 85%) were examined. ZnO was prepared by the base hydrolysis of zinc acetate in isopropanol using lithium hydroxide (LiOH · H2O) to hydrolyze the precursor. Sol-gel-based inorganic-organic hybrid polymers were modified with these oxides and were applied to cellulosic cotton (100%) and cotton/polyester (65/35%) fabrics. Inorganic-organic hybrids polymers were based on 3-glycidyloxypropyltrimethoxysilane (GPTMS). Bacteriological tests were performed in nutrient agar media on solid agar plates and in liquid broth systems using ZnO nanoparticles with average particle size of (40 nm). Our study showed the enhanced antibacterial activity of ZnO nanoparticles chitosan (different MW) of against a gram-negative bacterium Escherichia coli DSMZ 498 and a gram-positive Micrococcus luteus ATCC 9341 in repeated experiments. The antibacterial activity of textile treated with ZnO nanoparticles chitosan increases with decreasing the molecular weight of chitosan.

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Investigation of the antibacterial activity of the nanostructured system of copper sulfate in Na-carboxymethyl cellulose on the example of an indigenous strain of the gram-negative bacterium Esherichia coli

10.33920/igt-01-2104-02 ◽

2021 ◽

pp. 254-262

Author(s):

N.S. Lyakhovchenko ◽

V.Yu. Senchenkov ◽

A.Yu. Moldavanova ◽

D.A. Myagkov ◽

A.A. Sirotin ◽

...

Keyword(s):

Antibacterial Activity ◽

Copper Sulfate ◽

Active Material ◽

Weight Ratio ◽

Diffusion Method ◽

Negative Bacterium ◽

Disk Diffusion Method ◽

Gram Negative ◽

Esherichia Coli ◽

Biocidal Properties

The article provides a study of the antibacterial activity of nanostructured copper sulfate in Nacarboxymethyl cellulose on the example of an indigenous strain of the gram-negative bacterium Esherichia coli by the disk-diffusion method. The study revealed that the modification of the active material increases its biocidal properties by 321.34; 121.5; 369.5; 562.6 and 351.7% with an increase in the weight ratio “core : shell” to 1:2 and concentration from 0.5 to 1% in aqueous suspensions.

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New Pyrrolobenzoxazine Sesquiterpenoid Derivatives from the Fungus Talaromyces trachyspermus

Planta Medica ◽

10.1055/a-1392-1038 ◽

2021 ◽

Author(s):

Boonyanoot Chaiyosang ◽

Kwanjai Kanokmedhakul ◽

Kasem Soytong ◽

Supattra Poeaim ◽

Mayamor Soytong ◽

...

Keyword(s):

Antibacterial Activity ◽

Negative Bacterium ◽

Addition Compound ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Ic50 Values ◽

Hepg2 Cell Lines ◽

Weak Antibacterial Activity ◽

Ht 29 ◽

Mcf 7

AbstractThree new pyrrolobenzoxazine sesquiterpenoids, talatrachyoxazines A – C (1 – 3), together with fourteen known compounds (4 – 17), were isolated from the fungus Talaromyces trachyspermus EU23. Their structures were identified by spectroscopic evidence and mass spectrometry. The absolute configurations of 1 – 3 were determined by NOESY data and comparison of their calculated and experimental electronic circular dichroism (ECD) spectra. Compound 1 showed cytotoxic activity against HelaS3, KB, HT-29, MCF-7, and HepG2 cell lines with IC50 values of 7, 11, 10, 12, and 10 µM, respectively. Compounds 1 and 14 showed weak antibacterial activity against the gram-positive bacteria Bacillus cereus and Bacillus subtilis, while 1 – 3 and 14 showed weak antibacterial activity against the gram-negative bacterium Pseudomonas aeruginosa. In addition, compound 1 showed weak antibacterial activity against Escherichia coli.

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An investigation of the effect of the extracts from the seeds of Bidens pilosa on the growth of Escherichia coli and Bacillus subtilis

International Journal of Science and Research Archive ◽

10.30574/ijsra.2021.4.1.0191 ◽

2021 ◽

Vol 4 (1) ◽

pp. 125-128

Author(s):

Jordan Johnson ◽

Lovely Anderson ◽

Jennifer Laifa

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Food Poisoning ◽

Negative Bacterium ◽

Bidens Pilosa ◽

Gram Positive ◽

Gram Negative ◽

E Coli ◽

Zones Of Inhibition

Escherichia coli is a Gram-negative bacterium also found in the human. Bacillus subtilis is a Gram-positive, non-pathogenic, endospore-forming bacterium. The spores can survive the heat and B. subtilis to cause food poisoning. The study hypothesized that the extracts from B. pilosa would inhibit the growth of E. coli and B. subtilis. The seeds of B. pilosa were purchased from the seed company. The seeds were dried, ground, and shaken in acetone, ethanol, methanol, and water for 72 hours. Solvents were evaporated and the crude extracts were used for antibacterial activity using a modified Kirby-Bauer disc method. The results revealed that the growth of E. coli was inhibited by the extracts using ethanol. The zones of inhibition were 13 mm. The extracts that were extracted using acetone and water were not effective in inhibiting the growth of E. coli. The growth of B. subtilis was inhibited by the extracts from acetone and methanol. The zones of inhibition were 13 mm. The results revealed that the extracts from ethanol and water were not effective in inhibiting the growth of B. subtilis. Seeds of B. pilosa have the potential to be used as antimicrobials.

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The Effect of different crude methanolic extracts against some gram negative pathogenic bacteria

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2010.4.2.131 ◽

2010 ◽

Vol 4 (2) ◽

pp. 86-93

Author(s):

Hussain Ali Farhan

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Punica Granatum ◽

Gram Negative Bacteria ◽

Proteus Vulgaris ◽

Bacterial Strains ◽

Gram Negative ◽

Methanolic Extracts ◽

Crude Extracts

This present study was conducted to evaluated the antibacterial activity of crude extracts (Methanolic) of punica granatum peel, Menthe longifolia and Olea eurpeae leaves against some gram negative bacteria like Pseudomonas aerugenosa, Serratia marcesens, Escherichia coli, Proteus vulgaris, Klebsiella pnemunoniae. The Agar-well diffusion was used to know the ability of inhibititory of crude extracts against bacteria. Results showed different susceptibility response of bacteria under study. It was observed that Pseudomonas aerugenosa the most susceptible to Methanolic extracts to all plants in (25, 50) mg/ml-1 concentrate. While the bacterial strains shown no responding against mint and pomegranate extracts in 10 mg/ml-1 except Pseudomonas aerugenosa and Escherichia coli, was inhibited with Olive extracts only. That ability to inhabitation less in concentrate 10 mg ml-1.

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