scholarly journals Effects of Neck-Arm Restraint Suspension of Beef Carcasses on Meat Quality and Proteome of Different Muscles During Post-mortem Aging

2021 ◽  
Vol 8 ◽  
Author(s):  
Wentao Cai ◽  
Kaixin Wen ◽  
Leijie Che ◽  
Haijun Zhang ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Aging Time ◽  
Meat Quality ◽  
Shear Force ◽  
Post Mortem ◽  
Beef Quality ◽  
Beef Industry ◽  
Fragmentation Index ◽  
Beef Carcasses ◽  
Brown Cattle ◽  
The Cost

Beef quality is the first deciding factor for consumers to consider before purchasing. The aim of this study was to evaluate the effects of suspension and aging time on beef quality. We compared the differences in pH, drip loss, cooking loss, color, shear force, myofibril fragmentation index (MFI), and electron microscope of three muscle tissues between Achilles tendon (AT) and neck-arm restraint (NR) suspensions during seven aging periods (days 0, 1, 2, 3, 7, 14, and 21) after slaughter using the carcasses of six Xinjiang brown cattle. We found that NR suspension could significantly increase the water loss rate and MFI, as well as reduce the shear force compared to AT suspension. The muscle fiber structure with NR suspension was more severely damaged. The proteomics of longissimus dorsi was checked for the post-mortem days 1, 7, and 14. We detected 50, 26, and 29 differentially expressed proteins between NR and AT suspension at post-mortem days 1, 7, and 14, respectively. These proteins were involved in metabolic and muscle structure associated pathways and contributed to a comprehensive understanding of suspension-dependent meat quality regulation by proteins in beef cattle. To conclude, NR suspension can accelerate the aging time of beef carcasses, which will reduce the cost of carcass suspension and bring more benefits in the beef industry.

Effects of low-voltage electrical stimulation and aging on lamb meat quality

2012 ◽  
Vol 92 (1) ◽  
pp. 59-66 ◽  
Author(s):  
E. Pouliot ◽  
C. Gariépy ◽  
M. Thériault ◽  
C. Avezard ◽  
J. Fortin ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Aging Time ◽  
Meat Quality ◽  
Shear Force ◽  
Low Voltage ◽  
Ph Value ◽  
Target Weight ◽  
Lamb Meat ◽  
Fragmentation Index

Pouliot, E., Gariépy, C., Thériault, M., Avezard, C., Fortin, J., Simmons, N. J. and Castonguay, F. W. 2012. Effects of low-voltage electrical stimulation and aging on lamb meat quality. Can. J. Anim. Sci. 92: 59–66. The aim of this study was to evaluate the effect of electrical stimulation (ES) and aging time on meat quality of heavy lamb as produced in Quebec. Seventy-six Suffolk-sired crossbred male lambs were slaughtered at a target weight of 50±2 kg. Half of them were electrically stimulated (ES vs. control) at 5–10 min postmortem (21 V; 0.25 A; 60 s). Postmortem pH decline and temperature were monitored. After carcass cutting, longissimus dorsi sections were assigned to aging periods of 1, 3 or 8 d. Temperature decline was the same for both treatments (P=0.749). However, ES carcasses always had a lower pH value than controls during the first 24 h (P<0.001) while the ultimate pH was equivalent (P=0.803). Tenderness, as assessed by either Warner-Bratzler shear force (39 carcasses) or sensory evaluation (35 carcasses) was enhanced by both ES (P<0.001) and aging (P<0.001). At each aging time, tenderness was greater for ES meat. In addition, only 3 d of aging were necessary for ES meat to achieve the tenderness level attained by the controls after 8 d. Sarcomeres were longer (P<0.001) in ES meat than in controls while myofibrillar fragmentation index was not affected by ES treatment (P=0.743). Electrical stimulation also had small effects on color parameters (a*, b* and L*; P<0.01) and flavor (P=0.04). These results provide the first evidence that tenderness of the meat from heavy lambs produced and processed in Quebec could be enhanced by ES, mostly through cold shortening reduction.

scholarly journals How does the dietary cottonseed hull affect the carcass characteristics and meat quality of young bulls finished in a high-concentrate diet?

2016 ◽  
Vol 38 (3) ◽  
pp. 301 ◽  
Author(s):  
Carlos Emanuel Eiras ◽  
Mariana Garcia Ornaghi ◽  
Maribel Velandia Valero ◽  
Dayane Cristina Rivaroli ◽  
Ana Guerrero ◽  
...  
Keyword(s):  
Aging Time ◽  
Meat Quality ◽  
Shear Force ◽  
Carcass Characteristics ◽  
Total Lipids ◽  
Lean Beef ◽  
Concentrate System ◽  
Cottonseed Hull ◽  
Meat Aging

This study evaluated the effects of diets composed by cottonseed hull and meat aging on carcass characteristics and meat quality from young bulls fed on a high-concentrate system. Thirty crossbred bulls were assigned in a complete randomized experimental factorial design with three diets (CH21: cottonseed hull 210 g kg-1on a DM basis, CH27: cottonseed hull 270 g kg-1on a DM basis and CH33: cottonseed hull 330 g kg-1on a DM basis) and different aging times (24 hours and 3, 7 and 14 days). Meat from CH27 diets presented smaller LM moisture content (p < 0.05). Total lipids were smaller in CH33 diet (p <0.05). At 24 hours, CH21 diet presented smaller LM cooking loss than other diets. The increasing aging time reduced the shear force (p < 0.001) on the LM. Meat from CH27 diet presented the highest luminosity (p < 0.05) and yellowness values (p < 0.001). Three or seven aging days presented smaller values of LM luminosity. Likewise, the increasing aging time presented greater (p < 0.05) yellowness within the LM. Lipid oxidation was lower from CH21 diet (p < 0.001). The fatty acid composition on LM was similar among diets. The use of cottonseed hull could be useful strategies to improve the meat quality and lean beef production. 

scholarly journals Physicochemical properties of meat from Bos taurus and Bos indicus

2011 ◽  
Vol 40 (6) ◽  
pp. 1250-1259 ◽  
Author(s):  
Maria Cristina Bressan ◽  
Erika Cristina Rodrigues ◽  
Lizandra Vercezi Rossato ◽  
Eduardo Mendes Ramos ◽  
Luis Telo da Gama
Keyword(s):  
Physicochemical Properties ◽  
Meat Quality ◽  
Shear Force ◽  
Positive Relationship ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Post Mortem ◽  
Fresh Meat ◽  
Lower Shear ◽  
Before And After

The objective of this study was to characterize meat quality in Bos taurus and Bos indicus and to determine the influence of finishing system and genetic group on the physicochemical properties of the longissimus thoracis at 24 hours and 10 days post mortem (1ºC). The sample included 160 bulls of the B. taurus (n=75) and B. indicus (n=85) groups, finished either on pasture (n=46) or with grain supplementation (n=114), slaughtered at a carcass weight of 270 to 300 kg. Pasture-finished animals had higher pH, lower red content and a fat content 2.5 times lower than those finished with supplementation. Meat from supplement-finished animals had lower shear force in comparison to that from animals finished on pasture, with means of 7.7 and 8.5 kg at 24 hours, and of 5.5 and 5.9 kg at 10 days, respectively. Samples of B. taurus and B. indicus were similar in moisture and protein, but B. taurus presented higher means for ash and lower means for fat. Aged samples of B. indicus finished on pasture showed lower values for lightness and yellowness. Overall, lightness and yellow content increased and red content decreased with 10-day ageing. Means for shear force were lower in B. taurus than in B. indicus, with differences of 1.4 kg in fresh meat and 0.6 kg in aged samples. The reduction in shear force with ageing was more pronounced in samples with higher initial shear force, in spite of the positive relationship between shear force before and after ageing.

Electrical currents applied to lamb carcasses — effects on blood release and meat quality

2006 ◽  
Vol 46 (7) ◽  
pp. 885 ◽  
Author(s):  
D. L. Hopkins ◽  
F. D. Shaw ◽  
S. Baud ◽  
P. J. Walker
Keyword(s):  
Electric Current ◽  
Meat Quality ◽  
Early Phase ◽  
Shear Force ◽  
Control Group ◽  
Myofibrillar Proteins ◽  
Force Measurements ◽  
Electrical Currents ◽  
Treatment Groups ◽  
Fragmentation Index

The amount of collectable blood during the early phase of slaughter was determined for 48 lambs in 4 treatment groups: a control group (no current, no thoracic stick), a thoracic stick group only and groups subjected to a thoracic stick and either an electric current of 14 or 10 Hz frequency. The current was applied to the ‘skin-on’ carcass soon after the stunning/sticking process and subsequent to the thoracic stick, with a view to increasing the volume of blood released at that time. With both frequencies there was a small, but statistically significant (P<0.05), increase in the amount of released blood over non-stimulated treatments. Samples of M. longissimus thoracis et lumborum were aged for 1 and 5 days before freezing. Warner–Bratzler shear force measurements indicated the effectiveness of the current applications in reducing shear force with the 14 Hz waveform being superior to the 10 Hz waveform and in a related way the degradation of myofibrillar proteins was greater in meat subjected to the 14 Hz treatment when examined using the myofibrillar fragmentation index.

scholarly journals Effects of animal class and genotype on beef muscle nanostructure, pHu, colour and tenderness

2016 ◽  
Vol Volume 112 (Number 7/8) ◽  
Author(s):  
Amanda Y. Chulayo ◽  
Voster Muchenje ◽  
◽  
◽  
Keyword(s):  
Meat Quality ◽  
Shear Force ◽  
Quality Parameters ◽  
Muscle Fibres ◽  
Meat Tenderness ◽  
Beef Quality ◽  
Significant Relationships ◽  
Linear Relationships ◽  
Nguni Cattle ◽  
Skeletal Muscle Fibres

Abstract The objective of the study was to determine the effects of animal class and genotype of cattle on Muscularis longissimus thoracis et lumborum (LTL) nanostructure, ultimate pH (pHu), colour and tenderness of beef. We found significant positive relationships between distance travelled (DT) and meat temperature (Tm) (p less than 0.01); lairage duration (LDhr) and lightness of colour (L*) (p less than 0.01); ambient temperature (Ta) and L* (p less than 0.05) and LDhr and yellowness (b*) (p less than 0.05) of beef from Bonsmara cattle. Positive linear relationships were observed between DT and Tm (p less than 0.05) and DT and L* (p less than 0.01) of the non-descript cattle. There were no significant relationships between pre-slaughter stress and other beef quality parameters (pHu, Warner– Bratzler shear force [WBSF], redness [a*] and b*) of Bonsmara, Nguni and non-descript cattle. Muscle fibres differed among class and genotype and had an effect on meat quality. The Bonsmara, non-descript and Nguni cows and heifers had visible skeletal muscle fibres which were thin and long, promising improved tenderness of beef. Genotype and class had significant effects on meat quality parameters (Tm, pHu, L*, a*, b* and WBSF). The first important principal components as they appeared from the analysis were pHu, Tm, L*, a*, b* and WBSF. Therefore, animal class did not affect the nanostructure of beef; instead, meat tenderness was enhanced by the longer and visible muscle fibres. Nguni cattle produced meat of superior quality to that of the Bonsmara and the non-descript cattle.

Effect of growth rate on tenderness development and final tenderness of meat from Friesian calves

2002 ◽  
Vol 74 (2) ◽  
pp. 253-264 ◽  
Author(s):  
M. Therkildsen ◽  
L. Melchior Larsen ◽  
H. G. Bang ◽  
M. Vestergaard
Keyword(s):  
Growth Rate ◽  
Shear Force ◽  
High Growth ◽  
High Growth Rate ◽  
Eating Quality ◽  
Meat Tenderness ◽  
Post Mortem ◽  
Moderate Growth ◽  
Fragmentation Index ◽  
Calpain System

AbstractThe present study was conducted to determine the effect of growth rate of calves on the activity of the calpain system post mortem, post mortem desmin degradation, myofibrillar fragmentation index and meat tenderness of m. longissimus lumborum (LL) and m. supraspinatus (SS). Twenty-four Friesian heifer calves were allocated to two treatment groups: MM and HH. The MM calves were given food to achieve a moderate growth rate (678 g/day from 5 days of age to 90 kg body weight (BW) (period I) and 770 g/day from 90 kg BW to slaughter at 250 kg BW (period II)) and the HH calves were given food to achieve a high growth rate (period I: 895 g/day and period II: 1204 g/day). The myofibril fragmentation index (MFI), desmin degradation and the activity of µ-calpain, m-calpain and calpastatin were measured in LL and SS at slaughter, and 1 and 7 days post mortem. Shear force was measured in LL and SS after 1 and 7 days of ageing and a sensory panel evaluated the eating quality of the loin aged 7 days. MFI (P < 0·01) and tenderness (P < 0·01) were higher and shear force was lower (P < 0·001) in meat from HH calves compared with meat from MM calves. However, growth rate did not affect the activity of µ-calpain, m-calpain or calpastatin or the degradation pattern of desmin at any time post mortem. Besides growth rate and MFI at day 7 post mortem, which both correlated positively with meat tenderness (P < 0·001 and P < 0·01, respectively), fibre type traits and DNA or RNA concentrations were not generally related to final tenderness. Thus, it was concluded that increased growth rate of calves before slaughter increased the ease of fragmentation (MFI) of the meat and improved the tenderness but these findings could not be related to any changes in the activity of the calpain system.

Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs

2016 ◽  
Vol 96 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Seul Gi Kwon ◽  
Jung Hye Hwang ◽  
Da Hye Park ◽  
Tae Wan Kim ◽  
Deok Gyeong Kang ◽  
...  
Keyword(s):  
Moisture Content ◽  
Meat Quality ◽  
Shear Force ◽  
Cooking Loss ◽  
Quality Traits ◽  
Post Mortem ◽  
Single Nucleotide ◽  
Meat Quality Traits ◽  
Berkshire Pigs ◽  
Water Holding

Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G>A, which corresponds to 307G>R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH24h, meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH24h, CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meat-quality traits.

Very fast chilling of beef carcasses

2002 ◽  
Vol 82 (1) ◽  
pp. 59-67 ◽  
Author(s):  
J. L. Aalhus ◽  
W. M. Robertson ◽  
M. E. R. Dugan ◽  
D. R. Best
Keyword(s):  
Wind Speed ◽  
Key Words ◽  
Aging Time ◽  
Shear Forces ◽  
Beef Quality ◽  
Respective Control ◽  
Beef Carcasses ◽  
Extended Aging ◽  
Meat Colour ◽  
The Right

To determine if very fast carcass chilling (VFC; approximately –1°C within 5 h postmortem) of beef carcasses could be achieved through blast chilling, 96 carcasses of an estimated Canada 1 grade were used. The right side of each carcass was assigned to blast chilling conditions of either –20°C (48 sides) or –35°C (48 sides) while the left side was assigned to control chilling conditions (2°C for 24 h; 96 sides). Wind speed in the blast chill tunnel was 2.32 m s–1. Within each blast chill temperature, 12 blast-chilled sides were removed from the tunnel at each of four times (3, 5, 7 or 10 h) and chilled for the remainder of the 24-h period at 2°C. VFC conditions were not achieved in the deep hip region under any chilling regime, but were reached in the longissimus thoracis (LT) by approximately 6.5 h under rigorous chilling regimes (7 or 10 h of blast chilling at –35°C). After 6 d aging, LT muscles from sides blast chilled at –35°C for 10 h were more tender (22.4 vs. 29.3 N cm–2: P = 0.004) than those from their respective control sides. When these shear forces were categorized into tender (≤ 19.4 N cm–2), probably tender (> 19.4 ≤ 27.2 N cm–2), probably tough (> 27.2 = 33.2 N cm–2) and tough (> 33.2 N cm–2) categories, there was a higher proportion of tender (25.0 vs. 0.0%) and probably tender (58.33 vs. 33.33%) shears in the –35° C blast-chilled sides than in the control sides (P =0.06). However, these differences disappeared with extended aging to 21 d. Hence, the VFC advantage would be a reduction in the necessary aging time to achieve an acceptable product. The extreme chilling regime also resulted in significant reductions in cooler shrink, a slower rate of pH decline, an increased perception of marbling, darker meat colour and increased drip losses at retail. Further study of the mechanism of VFC tenderization is warranted. Key words: Beef carcasses, rapid chilling, beef quality, tenderness

scholarly journals Effect of Transportation and Low Voltage Electrical Stimulation on Meat Quality Characteristics of Omani Sheep

2010 ◽  
Vol 15 ◽  
pp. 1
Author(s):  
Isam T. Kadim ◽  
Osman Mahgoub ◽  
Waleed Al-Marzooqi ◽  
Samera Khalaf
Keyword(s):  
Electrical Stimulation ◽  
Meat Quality ◽  
Shear Force ◽  
Sarcomere Length ◽  
Low Voltage ◽  
Quality Characteristics ◽  
Cooking Loss ◽  
Longissimus Dorsi ◽  
Road Transportation ◽  
Fragmentation Index

The aim of this study was to determine the effects of road transportation during the hot season (36 oC) and low voltage electrical stimulation on meat quality characteristics of Omani sheep. Twenty intact male sheep (1-year old) were divided into two equal groups: 3 hrs transported or non-transported. The transported group was transferred to the slaughterhouse the day of slaughter in an open truck covering a distance of approximately 300 km. The non-transported group was kept in a lairage of a commercial slaughterhouse with ad libitum feed and water for 3 days prior to slaughter. Blood samples were collected from the animals before loading and prior to slaughter in order to assess their physiological response to stress in terms of hormonal levels. Fifty percent of the carcasses from each group were randomly assigned to low voltage (90 V) at 20 min postmortem. Muscle ultimate pH, expressed juice, cooking loss percentage, WB-shear force value, sarcomere length, myofibrillar fragmentation index and colour L*, a*, b* were measured on samples from Longissimus dorsi muscles collected 24 hrs postmortem at 2-4 oC. The transported sheep had significantly (P<0.05) higher cortisol adrenaline, nor-adrenaline, and dopamine levels than the non-transported group. Muscles from electrically-stimulated carcasses had significantly (P<0.05) lower pH values, longer sarcomere length, lower shear force value, higher expressed juice, myofibrillar fragmentation index and L* values than those from non-stimulated ones. Transportation significantly influenced meat quality characteristics of the Longissimus dorsi muscle. Muscle ultimate pH and shear force values were significantly higher, while CIE L*, a*, b*, expressed juice and cooking loss were lower in transported than non-transported sheep. This study indicated that pre-slaughter transportation at high ambient temperatures can cause noticeable changes in muscle physiology in sheep. Nevertheless, meat quality of transported sheep can be improved by electrical stimulation post-slaughter. 

Genotype and age effects on sheep meat production. 3. Meat quality

2007 ◽  
Vol 47 (10) ◽  
pp. 1155 ◽  
Author(s):  
D. L. Hopkins ◽  
D. F. Stanley ◽  
L. C. Martin ◽  
E. S. Toohey ◽  
A. R. Gilmour
Keyword(s):  
Meat Quality ◽  
Significant Interaction ◽  
Shear Force ◽  
Breeding Value ◽  
Meat Production ◽  
Post Mortem ◽  
Fat Percentage ◽  
Sheep Meat ◽  
Semimembranosus Muscle ◽  
Sire Breed

A 5 × 4 factorial experiment was designed in which lambs representing five genotypes were slaughtered at four ages (110, 236, 412 and 662 days of age or 4, 8, 14 and 22 months of age). The genotypes represented were Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M). In total, 595 animals were slaughtered and carcass traits, composition and meat quality measured. As the animals grew older, intramuscular fat percentage (IMF) increased in the longissimus (LL) muscle and as the proportion of Border Leicester parentage increased so did the levels of IMF. There was a significant sire breed effect on LL pH at 24 hours after slaughter (pH24), with Merino-sired animals having a higher pH24 than both Poll Dorset and BL-sired animals. A significant dam breed effect was found for pH24 of the semitendinosus (ST) muscle, with lambs born to Merino ewes having a higher pH than those born to BLM ewes. A significant effect of sire estimated breeding value (EBV) for yearling muscling (YEMD) on LL pH was detected indicating an increase in pH as the sire EBV for muscling increased. There was a significant Merino effect for ultimate pH of the ST, with Merino-sired animals having a higher ultimate pH than both Poll Dorset and Border Leicester-sired animals. Sucker lambs had a significantly lower ST pH than older animals. As animal age at slaughter increased, the LL became darker based on L* values. There was a significant increase in a* values as animals became older, with 14 and 22-month-old animals having redder muscle. As LL pH and YEMD EBV increased, a* values decreased. There was a significant interaction between animal age and post mortem aging time such that sucker lambs (4 months old) produced LL with the lowest shear force after 5 days of aging and exhibited the greatest decline in shear force with aging. There was a significant increase in semimembranosus muscle shear force for PDm-sired animals (mean ± s.e., 66.5 ± 1.99 Newtons) compared with other genotypes at 61.4 ± 1.28 Newtons, but this could not be attributed to YEMD.

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