The Prognostic Impact of the Metastatic Lymph Nodes Ratio in Colorectal Cancer

Abstract Background The function of ASXL1 protein in colorectal cancer has not been investigated yet. The purpose of this study was to investigate the clinicopathological and prognostic impact of ASXL1 protein expression on colorectal cancer.Methods We performed immunohistochemical staining of ASXL1 protein using tissue microarrays of 408 colorectal cancers, 46 normal colonic mucosae, 48 adenomas, and 92 metastatic lymph nodes. The intensity of expression was scored as 0–3, and the extent of staining was scored as 0–4, based on the percentage of positive cells. The immunoreactivity score (IRS) was calculated by multiplying the two scores.Results ASXL1 protein expression rates were 89.1% in normal mucosae, 72.9% in tubular adenomas, 44.4% in adenocarcinomas, and 28.3% in metastatic lymph nodes ( p < 0.001). With respect to the IRS cut-off score, the mean tumor size was smaller in the IRS 0–6 group than in the IRS 8–12 group (4.9 ± 2.1 vs. 6.3 ± 2.7 cm, p = 0.002). Lymph node metastasis was more frequent in the IRS 0–6 group than in the IRS 8–12 group (56.3% vs. 33.3%, p = 0.034). Lymphatic invasion was more frequent in the 0–6 group than in the IRS 8–12 group (56.0% vs. 33.3%, p = 0.035). The 5-year disease-free survival rate did not differ between two groups at stage II and stage III.Conclusions ASXL1 protein might act as a tumor suppressor in colorectal cancer. The loss of ASXL1 expression might be associated with metastasis via the lymphatic system to the lymph nodes.

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High Expression of sLex Associated with Poor Survival in Argentinian Colorectal Cancer Patients

The International Journal of Biological Markers ◽

10.5301/jbm.5000060 ◽

2014 ◽

Vol 29 (1) ◽

pp. e30-e39 ◽

Cited By ~ 5

Author(s):

Ariel Zwenger ◽

Martin Rabassa ◽

Sandra Demichelis ◽

Gabriel Grossman ◽

Amada Segal-Eiras ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Nodes ◽

Cancer Patients ◽

Normal Mucosa ◽

Poor Survival ◽

Primary Tumors ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph ◽

Colorectal Cancer Patients

Aim Colorectal cancer (CRC) is one of the most prevalent malignancies in Argentina with 11,043 new cases and 6,596 deaths estimated to have occurred in 2008. The present study was developed to clarify the differential expression of MUC1, MUC2, sLex, and sLea in colorectal cancer patients and their relationship with survival and clinical and histological features. Methods Ninety primary tumor samples and 43 metastatic lymph nodes from CRC patients were studied; follow-up was documented. Twenty-six adenoma and 68 histological normal mucosa specimens were analyzed. An immunohistochemical approach was applied and statistical analysis was performed. Results In tumor samples, MUC1, sLea, and sLex were highly expressed (94%, 67%, and 91%, respectively); also, we found a significantly increased expression of the 3 antigens in primary tumors and metastatic lymph nodes compared with normal mucosa and adenomas. MUC2 was expressed in 52% of both normal mucosa and CRC samples; this reactivity significantly decreased in metastatic lymph nodes (p<0.05). A multiple comparison analysis showed that MUC1 and sLex discriminated among 3 groups: normal, adenoma, and CRC tissues. The increase of sLex expression showed an association with recurrence, and survival analysis showed that a high sLex staining was significantly associated with a poor survival. By multivariate analysis MUC1 inmunoreactivity correlated positively and significantly with tumor size, while MUC2 expression showed the opposite correlation. Conclusions The correlation of sLex overexpression in primary tumors and metastatic lymph nodes, the discrimination among the normal, adenoma, and CRC groups based on sLex expression, as well as its association with recurrence and survival, all suggest a prognostic role of sLex in Argentinian CRC patients.

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MA12.09 Comparative Histological Subtype Analysis of Lung Adenocarcinoma Tumor and Metastatic Lymph Nodes and the Prognostic Impact

Journal of Thoracic Oncology ◽

10.1016/j.jtho.2016.11.476 ◽

2017 ◽

Vol 12 (1) ◽

pp. S414

Author(s):

Shaohua Lu ◽

Takashi Eguchi ◽

Zachary Tano ◽

Daniela Molena ◽

David Jones ◽

...

Keyword(s):

Lymph Nodes ◽

Lung Adenocarcinoma ◽

Prognostic Impact ◽

Histological Subtype ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph

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Polypeptide-GalNAc-Transferase-13 Shows Prognostic Impact in Breast Cancer

Cancers ◽

10.3390/cancers13225616 ◽

2021 ◽

Vol 13 (22) ◽

pp. 5616

Author(s):

Eugenia Fernandez ◽

Luis Ubillos ◽

Nabila Elgul ◽

María Florencia Festari ◽

Daniel Mazal ◽

...

Keyword(s):

Breast Cancer ◽

Lymph Nodes ◽

Cancer Biology ◽

Molecular Mechanisms ◽

Health Concern ◽

Breast Cancer Cell Lines ◽

Prognostic Impact ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph

Breast cancer is a public health concern and is currently the fifth cause of mortality worldwide. Identification of different biological subtypes is essential for clinical management; therefore, the role of pathologists is essential and useful tools for immunohistochemistry diagnosis are needed. Polypeptide-GalNAc-transferases are emerging novel biomarkers related to cancer behavior and GalNAc-T13, correlated with aggressiveness in some tumors, is an interesting candidate. Few monoclonal antibodies reacting with native proteins, and not affected by fixation and paraffin embedding, have been reported. The aim of this work was to develop a useful monoclonal antibody anti-GalNAc-T13 and to assess its potential significance in breast cancer diagnosis. We evaluated 6 human breast cancer cell lines, 338 primary breast tumors and 48 metastatic lymph nodes and looked for clinical significance correlating GalNAc-T13 expression with patients’ clinical features and survival. We found high GalNAc-T13 expression in 43.8% of the cases and observed a significant higher expression in metastatic lymph nodes, correlating with worse overall survival. We hypothesized several possible molecular mechanisms and their implications. We conclude that GalNAc-T13 may be a novel biomarker in breast cancer, useful for routine pathological diagnosis. Elucidation of molecular mechanisms related to aggressiveness should contribute to understand the role of GalNAc-T13 in breast cancer biology.

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The prognostic impact of neoadjuvant chemoradiotherapy on lymph node sampling in patients with locally advanced rectal cancer

Updates in Surgery ◽

10.1007/s13304-020-00841-3 ◽

2020 ◽

Vol 72 (3) ◽

pp. 793-800 ◽

Cited By ~ 2

Author(s):

Giovanni Li Destri ◽

Andrea Maugeri ◽

Alice Ramistella ◽

Gaetano La Greca ◽

Pietro Conti ◽

...

Keyword(s):

Rectal Cancer ◽

Lymph Node ◽

Lymph Nodes ◽

Locally Advanced ◽

Advanced Rectal Cancer ◽

Locally Advanced Rectal Cancer ◽

Prognostic Impact ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph ◽

Lymph Node Retrieval

Abstract According to the American Joint Committee on Cancer, at least 12 lymph nodes are required to accurately stage locally advanced rectal cancer (LARC). Neoadjuvant chemoradiation therapy (NACRT) reduces the number of lymph nodes retrieved during surgery. In this study, we evaluated the effect of NACRT on lymph node retrieval and prognosis in patients with LARC. We performed an observational study of 142 patients with LARC. Although our analysis was retrospective, data were collected prospectively. Half the patients were treated with NACRT and total mesorectal excision (TME) and the other half underwent TME only. The number of lymph nodes retrieved and the number of metastatic lymph nodes were significantly reduced in the NACRT group (P > 0.001). In the univariate and multivariate analyses, only NACRT and patient age were significantly associated with reduced lymph node retrieval. The number of metastatic lymph nodes and the lymph node ratio (LNR) both had a significant effect on prognosis when the patient population was examined as a whole (P = 0.003 and P = 0.001, respectively). However, the LNR was the only significant, independent prognostic factor in both treatment groups (P = 0.007 for the NACRT group; P = 0.04 for the no-NACRT group). NACRT improves patient prognosis only when the number of metastatic lymph nodes is reduced. The number of metastatic lymph nodes and the LNR are important prognostic factors. Lymph node retrieval remains an indispensable tool for staging and prognostic assessment of patients with rectal carcinoma treated with NACRT.

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Fibrosis in metastatic lymph nodes is clinically correlated to poor prognosis in colorectal cancer

Oncotarget ◽

10.18632/oncotarget.25636 ◽

2018 ◽

Vol 9 (51) ◽

pp. 29574-29586 ◽

Cited By ~ 7

Author(s):

Daiji Ikuta ◽

Toru Miyake ◽

Tomoharu Shimizu ◽

Hiromichi Sonoda ◽

Ken-Ichi Mukaisho ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Nodes ◽

Poor Prognosis ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph

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The number of metastatic lymph nodes exhibiting poorly differentiated clusters predicts survival in patients with pStage III colorectal cancer

International Journal of Colorectal Disease ◽

10.1007/s00384-015-2393-5 ◽

2015 ◽

Vol 31 (2) ◽

pp. 283-290 ◽

Cited By ~ 7

Author(s):

Osamu Kinoshita ◽

Mitsuo Kishimoto ◽

Yasutoshi Murayama ◽

Yoshiaki Kuriu ◽

Masayoshi Nakanishi ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Nodes ◽

Metastatic Lymph Nodes ◽

Metastatic Lymph ◽

Poorly Differentiated Clusters ◽

Poorly Differentiated

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A prospective study of dual-energy CT in determining the metastatic and non-metastatic lymph nodes of colorectal cancer

10.21203/rs.3.rs-44884/v1 ◽

2020 ◽

Author(s):

Lin Qiu ◽

Junjiao Hu ◽

Zeping Weng ◽

Fasheng Li ◽

Fei Wang ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Nodes ◽

Electron Density ◽

Sensitivity And Specificity ◽

Dual Energy ◽

Dual Energy Ct ◽

Metastatic Node ◽

Metastatic Lymph Nodes ◽

Quantitative Parameters ◽

Metastatic Lymph

Abstract Background To explore the ability of Dual-energy CT (DECT) to differentiate metastatic from non-metastatic lymph nodes in colorectal cancer (CRC). Methods Seventy-one patients with primary CRC underwent contrast-enhanced DECT imaging before surgery. The colorectal specimen was scanned after surgery, and lymph nodes were matched to the pathology report. The DECT quantitative parameters were analyzed: dual-energy curve slope value(λHU), standardized iodine concentration (n△HU), iodine water ratio (nIWR), electron density value (nρeff), and effective atom-number (nZ), for the metastatic and non-metastatic lymph node differentiation. Also, sensitivity and specificity analyses were performed by using receiver operating characteristic curve. Results One hundred and fifty lymph nodes including 66 non-metastatic and 84 metastatic lymph nodes were matched using the radiological-pathological correlation. Metastatic node had a significantly greater λHU, n△HU and nIWR values than non-metastatic node in both arterial and venous phases (P < 0.01). The AUC, sensitivity and specificity were 0.80, 80.30% and 65.48% for λHU; 0.86, 69.70% and 95.24% for n△HU; 0.88, 71.21% and 95.24% for nIWR in the arterial phase. No significant difference was found in electron density and effective Z value for differentiation. Conclusion Dual-energy CT quantitative parameters may be helpful in diagnosing metastatic lymph nodes of CRC.

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A Study of the Correlation Between M2 Macrophages and Lymph Node Metastasis of Colorectal Cancer

10.21203/rs.3.rs-136192/v1 ◽

2020 ◽

Author(s):

Yanping Wang ◽

Jikun Wang ◽

Jinhao Liu ◽

Zuoxiu Shi ◽

Yanlei Chen ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Lymph Nodes ◽

Comprehensive Treatment ◽

M2 Macrophage ◽

M2 Macrophages ◽

Metastatic Lymph Nodes ◽

Node Metastasis ◽

Metastatic Lymph

Abstract Background: Lymph node metastasis is a major prognostic factor of colorectal cancer and an important indicator for individualized treatment. M2 macrophages play a key role in carcinogenesis and tumor development, not only enhancing invasiveness, but also promoting lymph node metastasis. The purpose of this study was to investigate the effect of CD163-positive M2 macrophages on lymph node metastasis in colorectal cancer.Methods: Postoperative lymph node tissues were obtained from 120 patients with colorectal cancer who underwent radical surgery in the First Affiliated Hospital of Jinzhou Medical University between December 2019 and May 2020. We detected the expression of the CD163 protein in lymph nodes by immunohistochemistry. Furthermore, the relationship between M2 macrophages identified by this marker and lymph node metastasis were analyzed using the independent sample T-test and Chi-square test.Results: M2 macrophages were increased not only in metastatic lymph nodes, but also in non-metastatic lymph nodes adjacent to the cancer. The M2 macrophage count was higher in patients with macro-metastases than in those with micro-metastases.Conclusions: M2 macrophages represent an important factor for the promotion of lymph node metastasis in colorectal cancer, and may be a potential marker for its prediction. This may offer a new target for the comprehensive treatment of colorectal cancer.

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