scholarly journals Quality Evaluation of Juniperus rigida Sieb. et Zucc. Based on Phenolic Profiles, Bioactivity, and HPLC Fingerprint Combined with Chemometrics

2017 ◽  
Vol 8 ◽  
Author(s):  
Zehua Liu ◽  
Dongmei Wang ◽  
Dengwu Li ◽  
Shuai Zhang
Keyword(s):  
Quality Evaluation ◽  
Hplc Fingerprint ◽  
Phenolic Profiles

scholarly journals Chromatographic Fingerprint Combined with Content of Asperosaponin VI and Antioxidant Activity for Quality Evaluation of Wine-fried Dipsaci Radix

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Li Song ◽  
Shaoyun Wang ◽  
Xiaoju Duan ◽  
Xinhua Liu ◽  
Zhaofang Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Quality Evaluation ◽  
Heating Temperature ◽  
Processing Condition ◽  
Influential Factor ◽  
Multivariate Statistical ◽  
Factors Affecting ◽  
Hplc Fingerprint

Dipsaci Radix, the dry root of Dipsacusasper Wall. ex Henry, is a commonly used traditional Chinese medicine (TCM). A novel comprehensive method was proposed for quality evaluation of wine-fried Dipsaci Radix by an integrated data including three aspects of information: high performance liquid chromatography (HPLC) fingerprint, asperosaponin VI content and antioxidant activity (AA). Various indicators including fingerprint similarity, asperosaponin VI content and AA were respectively employed for quality assessment of processed Dipsaci Radix samples. Results showed that considerable differences existed in quality of processed samples with different processing conditions according to three indicators. Among the factors affecting quality of wine-fried Dipsaci Radix, heating temperature was the most influential factor based on analysis of variance (ANOVA), and should be cautiously controlled. The three evaluation indicators respectively used for optimization of processing technology suggested different optimal conditions of wine-frying. Therefore, a combined indicator based on three evaluation indicators was used for determination of optimal processing condition. Multivariate statistical methods such as Hierarchical Clustering Analysis (HCA) and Principal Components Analysis (PCA) were both employed to classify the processed samples for quality evaluation. To more comprehensively evaluate the quality of wine-fried Dipsaci Radix, HPLC fingerprint combined with content of asperosaponin VI and AA may be a reasonable and practical approach.

scholarly journals Development of HPLC Fingerprint for Quality Assessment of Bulbus Lilii

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801
Author(s):  
Kunming Qin ◽  
Hao Cai ◽  
Lijuan Zheng ◽  
Miao Zhang ◽  
Xinghai Zhang ◽  
...  
Keyword(s):  
High Performance ◽  
Quality Evaluation ◽  
Quality Analysis ◽  
Analysis Method ◽  
Hplc Fingerprint ◽  
Array Detection ◽  
Different Origins ◽  
First Time ◽  
And Storage

In this study, a simple, sensitive and reliable fingerprint analysis method by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for quality analysis of Bulbus Lilii, which is a famous traditional Chinese medicine and widely used as a restorative food. Then the developed method was subsequently applied to analyse 16 samples collected or purchased from different origins. Twelve common fingerprint peaks, including four definitely identified peaks, were found in total, and were involved for further characterisation and quality evaluation of Bulbus Lilii. The chemical quality of commercial samples collected from different origins varied obviously, indicating that more attention should be paid to the standardisation of the process of harvesting, manufacturing and storage of Bulbus Lilii. This is the first time that a new method has been established to control the quality of Bulbus Lilii through HPLC-DAD.

scholarly journals Comprehensive Investigation of the Differences of the Roots of Wild and Cultivated Mirabilis himalaica (Edgew) Heim Based on Macroscopic and Microscopic Identification Using HPLC Fingerprint

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Yuanyang Shao ◽  
Lian Peng ◽  
Hui Lin ◽  
Jiahui Li ◽  
Yuetong Yu ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Evaluation Method ◽  
Quality Standards ◽  
Clinical Applications ◽  
Tibetan Medicine ◽  
Chemical Components ◽  
Comprehensive Investigation ◽  
Hplc Fingerprint ◽  
Microscopic Identification ◽  
Further Development

Mirabilishimalaica (Edgew) Heim (MH) is an important Tibetan medicine with demonstrated medicinal efficacy and promising developmental value. A previous study of MH was limited to vague morphological and microscopic descriptions, restricting its clinical application and further development as a medicine. The goal of this study was to comprehensively characterize wild and cultivated products of MH using macroscopic and microscopic identification using HPLC fingerprint. The results revealed that the cultivated and wild MH exhibited differences in macroscopic and microscopic characteristics and chemical components. This analysis can facilitate the establishment of a more comprehensive quality evaluation method for MH. These results provide the basis for clinical applications and the improvement of quality standards of MH as a step towards modernization of Tibetan medicine.

scholarly journals HPLC Fingerprint Combined With Multicomponent Quantification as an Efficient Method for Quality Evaluation of Pharbitidis Semen

2020 ◽  
Vol 15 (6) ◽  
pp. 1934578X2093164
Author(s):  
Huiwei Bao ◽  
Huailei Yang ◽  
Feng Wang ◽  
Kaixuan Zhou ◽  
Yanan Yang ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Caffeic Acid ◽  
Evaluation System ◽  
High Performance ◽  
Quality Evaluation ◽  
Hplc Method ◽  
Hplc Fingerprint ◽  
Protocatechuic Aldehyde ◽  
Effective Components

Pharbitidis Semen is a traditional Chinese medicine(TCM) with a long history for treatment of edema and fullness, fecal and urinary retention, phlegm and retained fluid, and abdominal pain due to parasitic infestation. Since Pharbitidis Semen is distributed throughout the country, the quality of the medicine from different origins may be varied. Moreover, the reported method could not control the quality comprehensively. In this article, a fingerprint of Pharbitidis Semen has been established based on a high-performance liquid chromatography (HPLC) method. In addition, the contents of the 2 main effective components were determined simultaneously. The reference HPLC fingerprint was obtained according to the chromatograms of test samples. The similarity values were calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A edition). Cluster analysis of 10 batches of samples was performed using statistical software (SPSS 20.0). The HPLC fingerprints of 10 batches of Pharbitidis Semen showed 25 well-resolved common peaks in each chromatogram. Two of these peaks were assigned to protocatechuic aldehyde and caffeic acid. As a result, HPLC fingerprint similarities of 10 batches of samples were more than 0.99. Pharbitidis Semen from different habitats could be divided into 3 or 2 groups. The results of cluster analysis showed that samples classified into 1 group were associated with their habitats and breeds. At the same time, quantification results showed that the contents of protocatechuic aldehyde and caffeic acid were in the range of 0.026-0.088 and 0.019-0.053 mg/g−1 respectively. HPLC fingerprint combined with multicomponent quantification and data analysis techniques can be an efficient and useful method for monitoring the quality of Pharbitidis Semen. This study also provides a practical strategy for overall quality evaluation and control of traditional Chinese medicines.

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