scholarly journals Exploiting Genic Male Sterility in Rice: From Molecular Dissection to Breeding Applications

2021 ◽  
Vol 12 ◽  
Author(s):  
Adil Abbas ◽  
Ping Yu ◽  
Lianping Sun ◽  
Zhengfu Yang ◽  
Daibo Chen ◽  
...  
Keyword(s):  
Male Sterility ◽  
Crop Production ◽  
Regulatory Networks ◽  
Crop Improvement ◽  
Rice Breeding ◽  
Male Sterile ◽  
Rice Varieties ◽  
Genic Male Sterility ◽  
Global Food Security

Rice (Oryza sativa L.) occupies a very salient and indispensable status among cereal crops, as its vast production is used to feed nearly half of the world’s population. Male sterile plants are the fundamental breeding materials needed for specific propagation in order to meet the elevated current food demands. The development of the rice varieties with desired traits has become the ultimate need of the time. Genic male sterility is a predominant system that is vastly deployed and exploited for crop improvement. Hence, the identification of new genetic elements and the cognizance of the underlying regulatory networks affecting male sterility in rice are crucial to harness heterosis and ensure global food security. Over the years, a variety of genomics studies have uncovered numerous mechanisms regulating male sterility in rice, which provided a deeper and wider understanding on the complex molecular basis of anther and pollen development. The recent advances in genomics and the emergence of multiple biotechnological methods have revolutionized the field of rice breeding. In this review, we have briefly documented the recent evolution, exploration, and exploitation of genic male sterility to the improvement of rice crop production. Furthermore, this review describes future perspectives with focus on state-of-the-art developments in the engineering of male sterility to overcome issues associated with male sterility-mediated rice breeding to address the current challenges. Finally, we provide our perspectives on diversified studies regarding the identification and characterization of genic male sterility genes, the development of new biotechnology-based male sterility systems, and their integrated applications for hybrid rice breeding.

scholarly journals Characterization of the Male-sterile Line Bcajh97-01A/B and Identification of Candidate Genes for Genic Male Sterility in Chinese Cabbage-pak-choi

2009 ◽  
Vol 134 (6) ◽  
pp. 632-640 ◽  
Author(s):  
Li Huang ◽  
Wan-zhi Ye ◽  
Ting-ting Liu ◽  
Jia-shu Cao
Keyword(s):  
Male Sterility ◽  
Candidate Genes ◽  
Chinese Cabbage ◽  
Developmental Stages ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Flower Buds ◽  
Pak Choi ◽  
Genic Male Sterility

Cytological features of ‘Aijiaohuang’ chinese cabbage-pak-choi (Brassica campestris ssp. chinensis) Bcajh97-01A/B genic male-sterile AB line were examined to determine phenotypic reasons for male sterility. The sterile line Bcajh97-01A was found to undergo aberrant cytokinesis during male meiosis. Transcriptional profiling of the flower buds of both fertile and sterile plants was performed at the periods preceding meiosis, at the tetrad to uninucleate pollen period, and at the binucleate to mature pollen period. Transcript-derived fragments (TDFs) from corresponding genes that were expressed in flower buds at these three different stages could be divided into nine classes. We sequenced a total of 14 new TDFs that were differentially displayed at particular pollen developmental stages, including eight genes with unknown or hypothetical functions and six genes showing significant homology with known genes. This characterization of the Bcajh97-01A genic male-sterile line allowed the identification of candidate genes underlying genic male sterility.

scholarly journals Reproductive characterization of interspecific hybrids among Capsicum species

2011 ◽  
Vol 11 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Carlos Eduardo da Silva Monteiro ◽  
Telma Nair Santana Pereira ◽  
Karina Pereira de Campos
Keyword(s):  
Male Sterility ◽  
Interspecific Hybrids ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Male Sterile ◽  
Viable Pollen ◽  
Hybrid Combination ◽  
Capsicum Species ◽  
The Cross

The objective of this study was the reproductive characterization of Capsicum accessions as well as of interspecific hybrids, based on pollen viability. Hybrids were obtained between Capsicum species. Pollen viability was high in most accessions, indicating that meiosis is normal, resulting in viable pollen grains. The pollen viability of species C. pubescens was the lowest (27 %). The interspecific hybrids had varying degrees of pollen viability, from fertile combinations (C. chinense x C. frutescens and C. annuum x C. baccatum) to male sterile combinations. Pollen viability also varied within the hybrid combination according to accessions used in the cross. Results indicate that male sterility is one of the incompatibility barriers among Capsicum species since hybrids can be established, but may be male sterile.

scholarly journals Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene

2016 ◽  
Vol 113 (49) ◽  
pp. 14145-14150 ◽  
Author(s):  
Zhenyi Chang ◽  
Zhufeng Chen ◽  
Na Wang ◽  
Gang Xie ◽  
Jiawei Lu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Environmental Conditions ◽  
Hybrid Rice ◽  
Rice Breeding ◽  
Male Sterile ◽  
Red Fluorescence ◽  
Cytoplasmic Male Sterile ◽  
Cross Pollination ◽  
Self Pollination

The breeding and large-scale adoption of hybrid seeds is an important achievement in agriculture. Rice hybrid seed production uses cytoplasmic male sterile lines or photoperiod/thermo-sensitive genic male sterile lines (PTGMS) as female parent. Cytoplasmic male sterile lines are propagated via cross-pollination by corresponding maintainer lines, whereas PTGMS lines are propagated via self-pollination under environmental conditions restoring male fertility. Despite huge successes, both systems have their intrinsic drawbacks. Here, we constructed a rice male sterility system using a nuclear gene named Oryza sativa No Pollen 1 (OsNP1). OsNP1 encodes a putative glucose–methanol–choline oxidoreductase regulating tapetum degeneration and pollen exine formation; it is specifically expressed in the tapetum and miscrospores. The osnp1 mutant plant displays normal vegetative growth but complete male sterility insensitive to environmental conditions. OsNP1 was coupled with an α-amylase gene to devitalize transgenic pollen and the red fluorescence protein (DsRed) gene to mark transgenic seed and transformed into the osnp1 mutant. Self-pollination of the transgenic plant carrying a single hemizygous transgene produced nontransgenic male sterile and transgenic fertile seeds in 1:1 ratio that can be sorted out based on the red fluorescence coded by DsRed. Cross-pollination of the fertile transgenic plants to the nontransgenic male sterile plants propagated the male sterile seeds of high purity. The male sterile line was crossed with ∼1,200 individual rice germplasms available. Approximately 85% of the F1s outperformed their parents in per plant yield, and 10% out-yielded the best local cultivars, indicating that the technology is promising in hybrid rice breeding and production.

scholarly journals GENETIC STUDIES ON THE PHOTOTHERMO SENSITIVE GENIC MALE STERILITY (PTGMS) AND ITS UTILIZATION IN RICE BREEDING.

2008 ◽  
Vol 33 (5) ◽  
pp. 3391-3404
Author(s):  
Z. El-Diasty ◽  
H. El-Mowafi ◽  
M. Hammada ◽  
R. Abdallah
Keyword(s):  
Male Sterility ◽  
Rice Breeding ◽  
Genetic Studies ◽  
Genic Male Sterility

scholarly journals CHARACTERIZATION OF A MALE-STERILE SYSTEM WITH A CLOSELY LINKED SEEDLING MARKER TO FACILITATE F1 HYBRID TOMATO SEED PRODUCTION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1168e-1168 ◽  
Author(s):  
Edward C. Tigchelaar
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Marker Gene ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Chromosome 2 ◽  
Male Sterile ◽  
Tomato Seed ◽  
F1 Hybrid

The coupling phase linkages have been synthesized between the gene aw (without anthocyanin) and the male sterile gene ms15 (and its alleles ms26, ms47, and an Israeli source of male sterility). Less than 2 map units separate aw and ms15 on chromosome 2, providing a convenient seedling marker gene to rapidly identify male sterility for both inbred development and hybrid seed production. The seedling marker also provides a convenient marker to rapidly assess hybrid seed purity. Unique features of each of the alleles involved in male sterility and their use in inbred and hybrid development will be described.

scholarly journals OsmiR396/Growth Regulating Factor modulate rice grain size through direct regulation of embryo-specific miR408

2021 ◽  
Author(s):  
Xiaofang Yang ◽  
Xiaoling Zhao ◽  
Zhengyan Dai ◽  
Feilong Ma ◽  
Xuexia Miao ◽  
...  
Keyword(s):  
Grain Size ◽  
Regulatory Networks ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Expression Patterns ◽  
Crop Improvement ◽  
Genetic Regulatory Networks ◽  
Rice Grain ◽  
Rice Varieties ◽  
Size Regulation

Abstract microRNAs (miRNAs) are promising targets for crop improvement of complex agricultural traits. Coordinated activity between/among different miRNAs may fine-tune specific developmental processes in diverse organisms. Grain size is a main factor determining rice (Oryza sativa L.) crop yield, but the network of miRNAs influencing this trait remains uncharacterized. Here we show that sequestering OsmiR396 through target mimicry (MIM396) can substantially increase grain size in several japonica and indica rice subspecies and in plants with excessive tillers and a high panicle density. Thus, OsmiR396 has a major role related to the regulation of rice grain size. The grain shape of Growth Regulating Factor8 (OsGRF8)-overexpressing transgenic plants was most similar to that of MIM396 plants, suggesting OsGRF8 is a major mediator of OsmiR396 in grain size regulation. A miRNA microarray analysis revealed changes to the expression of many miRNAs, including OsmiR408, in the MIM396 plants. Analyses of gene expression patterns and functions indicated OsmiR408 is an embryo-specific miRNA that positively regulates grain size. Silencing OsmiR408 expression (miR408KO) using CRISPR technology resulted in small grains. Moreover, we revealed the direct regulatory effects of OsGRF8 on OsMIR408 expression. A genetic analysis further showed that the large-grain phenotype of MIM396 plants could be complemented by miR408KO. Also, several hormone signaling pathways might be involved in the OsmiR396/GRF-meditated grain size regulation. Our findings suggest that genetic regulatory networks comprising various miRNAs, such as OsmiR396 and OsmiR408, may be crucial for controlling rice grain size. Furthermore, the OsmiR396/GRF module may be important for breeding new high-yielding rice varieties.

Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed

2007 ◽  
Vol 0 (0) ◽  
pp. 070920220336001-???
Author(s):  
D. F. Hong ◽  
J. Liu ◽  
G. S. Yang ◽  
Q. B. He
Keyword(s):  
Male Sterility ◽  
Scar Markers ◽  
Genic Male Sterility ◽  
Dominant Genic Male Sterility

scholarly journals The Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 912
Author(s):  
Huirong Dong ◽  
Yong Huang ◽  
Kejian Wang
Keyword(s):  
Herbicide Resistance ◽  
Crop Production ◽  
Gene Editing ◽  
Crop Improvement ◽  
Global Food Security ◽  
Herbicide Resistant ◽  
Transgenic Breeding ◽  
Short Palindromic Repeat ◽  
Global Food ◽  
Huge Challenge

The rapid increase in herbicide-resistant weeds creates a huge challenge to global food security because it can reduce crop production, causing considerable losses. Combined with a lack of novel herbicides, cultivating herbicide-resistant crops becomes an effective strategy to control weeds because of reduced crop phytotoxicity, and it expands the herbicidal spectrum. Recently developed clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas)-mediated genome editing techniques enable efficiently targeted modification and hold great potential in creating desired plants with herbicide resistance. In the present review, we briefly summarize the mechanism responsible for herbicide resistance in plants and then discuss the applications of traditional mutagenesis and transgenic breeding in cultivating herbicide-resistant crops. We mainly emphasize the development and use of CRISPR/Cas technology in herbicide-resistant crop improvement. Finally, we discuss the future applications of the CRISPR/Cas system for developing herbicide-resistant crops.

scholarly journals Characterization of the Mitochondrial Genome of a Wheat AL-Type Male Sterility Line and the Candidate CMS Gene

2021 ◽  
Vol 22 (12) ◽  
pp. 6388
Author(s):  
Miaomiao Hao ◽  
Wenlong Yang ◽  
Weiwen Lu ◽  
Linhe Sun ◽  
Muhammad Shoaib ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterility ◽  
Seed Production ◽  
Structure Prediction ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Cms Line ◽  
Generation Sequencing

Heterosis utilization is very important in hybrid seed production. An AL-type cytoplasmic male sterile (CMS) line has been used in wheat-hybrid seed production, but its sterility mechanism has not been explored. In the present study, we sequenced and verified the candidate CMS gene in the AL-type sterile line (AL18A) and its maintainer line (AL18B). In the late uni-nucleate stage, the tapetum cells of AL18A showed delayed programmed cell death (PCD) and termination of microspore at the bi-nucleate stage. As compared to AL18B, the AL18A line produced 100% aborted pollens. The mitochondrial genomes of AL18A and AL18B were sequenced using the next generation sequencing such as Hiseq and PacBio. It was found that the mitochondrial genome of AL18A had 99% similarity with that of Triticum timopheevii, AL18B was identical to that of Triticum aestivum cv. Chinese Yumai. Based on transmembrane structure prediction, 12 orfs were selected as candidate CMS genes, including a previously suggested orf256. Only the lines harboring orf279 showed sterility in the transgenic Arabidopsis system, indicating that orf279 is the CMS gene in the AL-type wheat CMS lines. These results provide a theoretical basis and data support to further analyze the mechanism of AL-type cytoplasmic male sterility in wheat.

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