Mechanism of Resistance to S-metolachlor in Palmer amaranth

Herbicides are major tools for effective weed management. The evolution of resistance to herbicides in weedy species, especially contributed by non-target-site-based resistance (NTSR) is a worrisome issue in crop production globally. Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) is one of the extremely difficult weeds in southern US crop production. In this study, we present the level and molecular basis of resistance to the chloroacetamide herbicide, S-metolachlor, in six field-evolved A. palmeri populations that had survivors at the recommended field-dose (1.1 kg ai ha−1). These samples were collected in 2014 and 2015. The level of resistance was determined in dose-response assays. The effective dose for 50% control (ED50) of the susceptible population was 27 g ai ha−1, whereas the ED50 of the resistant populations ranged from 88 to 785 g ai ha−1. Therefore, A. palmeri resistance to S-metolachlor evolved in Arkansas as early as 2014. Metabolic-inhibitor and molecular assays indicated NTSR in these populations, mainly driven by GSTs. To understand the mechanism of resistance, selected candidate genes were analyzed in leaves and roots of survivors (with 1 × S-metolachlor). Expression analysis of the candidate genes showed that the primary site of S-metolachlor detoxification in A. palmeri is in the roots. Two GST genes, ApGSTU19 and ApGSTF8 were constitutively highly expressed in roots of all plants across all resistant populations tested. The expression of both GSTs increased further in survivors after treatment with S-metolachlor. The induction level of ApGSTF2 and ApGSTF2like by S-metolachlor differed among resistant populations. Overall, higher expression of ApGSTU19, ApGSTF8, ApGSTF2, and ApGSTF2like, which would lead to higher GST activity in roots, was strongly associated with the resistant phenotype. Phylogenetic relationship and analysis of substrate binding site of candidate genes suggested functional similarities with known metolachlor-detoxifying GSTs, effecting metabolic resistance to S-metolachlor in A. palmeri. Resistance is achieved by elevated baseline expression of these genes and further induction by S-metolachlor in resistant plants.

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Effect of dicamba rate and application parameters on protoporphyrinogen oxidase inhibitor-resistant and -susceptible Palmer amaranth (Amaranthus palmeri) control

Weed Technology ◽

10.1017/wet.2020.59 ◽

2020 ◽

pp. 1-5

Author(s):

Wyatt Coffman ◽

Tom Barber ◽

Jason K. Norsworthy ◽

Greg R. Kruger

Keyword(s):

Palmer Amaranth ◽

Research Station ◽

Spray Parameters ◽

Group 14 ◽

Metabolic Resistance ◽

Susceptible Population ◽

Protoporphyrinogen Oxidase ◽

Sites Of Action ◽

Carrier Volume ◽

Nozzle Type

Abstract Throughout eastern Arkansas, Palmer amaranth resistant to protoporphyrinogen oxidase (PPO)-inhibiting herbicides (Group 14 herbicides) has become widespread. Most PPO-resistant Palmer amaranth biotypes possess a target-site mutation, but a metabolic resistance mechanism to fomesafen (Group 14) has also been identified. Once metabolic resistance manifests, plants may also be tolerant to other herbicides and sites of action. To evaluate whether varying spray parameters affected control of PPO-resistant Palmer amaranth in dicamba-tolerant crops, field trials were conducted in 2017 and 2018 at the Lon Mann Cotton Research Station near Marianna, AR, and on-farm in Marion, AR. The experiment included split plot factors of dicamba rate, nozzle type, and carrier volume, with a whole plot factor of population. Dicamba was applied at 560 or 1120 g ae ha−1 through 110015 TTI or AirMix nozzles at 70 or 140 L ha−1 to PPO-resistant or PPO-susceptible Palmer amaranth. Palmer amaranth control 14 d after treatment (DAT) was influenced by an interaction between population and carrier volume. PPO-resistant Palmer amaranth control 14 DAT was 81% regardless of carrier volume, compared with 90% and 95% control at 70 and 140 L ha−1, respectively, of the PPO-susceptible population. An interaction between nozzle type and carrier volume influenced Palmer amaranth control 21 DAT, whereas AirMix nozzles at 140 L ha−1 controlled Palmer amaranth at a greater level (94%) than any other nozzle and carrier volume combination (≤90%). An interaction between population and dicamba rate influenced the relative density of Palmer amaranth 21 DAT. PPO-resistant Palmer amaranth density was less affected by dicamba at either rate than PPO-susceptible Palmer amaranth, relative to the nontreated check. Results concur with those of other research that suggest PPO-resistant Palmer amaranth is harder to control with dicamba. Otherwise, increasing carrier volume affected overall Palmer amaranth control to a greater degree than any other factor.

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Diverse Approaches to Herbicide-Resistant Weed Management

Weed Science ◽

10.1614/ws-d-15-00117.1 ◽

2016 ◽

Vol 64 (SP1) ◽

pp. 570-584 ◽

Cited By ~ 44

Author(s):

Micheal D. K. Owen

Keyword(s):

Weed Management ◽

Crop Production ◽

Production Systems ◽

Cost Effective ◽

Developed Countries ◽

Weed Species ◽

Herbicide Resistant ◽

Cost Effective Method ◽

Evolution Of Resistance ◽

Complexity Cost

Herbicides have been the principal means of weed control in developed countries for approximately 50 yr because they are the most cost-effective method. Such general use of herbicides has resulted in weed resistance to herbicides, which continues to be a growing problem. Within the past decade, the evolution of resistance to the once-dominant herbicide glyphosate has resulted in major concerns about the future ability to control weeds in many crop systems. Moreover, many weed species have evolved resistance to multiple mechanisms of herbicide action. Given the dearth of new herbicides with novel mechanisms of action, it appears inevitable that weed management programs will need to be supplemented by the use of tactics other than herbicides. However, the inclusion of more diversity for weed management also introduces complexity, cost, and time constraints to current crop production systems. This paper describes broadly the considerations, opportunities, and constraints of diverse weed management tactics to address the burgeoning problems with herbicide resistance.

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PAM: Decision Support for Long-Term Palmer Amaranth (Amaranthus palmeri) Control

Weed Technology ◽

10.1017/wet.2017.69 ◽

2017 ◽

Vol 31 (6) ◽

pp. 915-927 ◽

Cited By ~ 9

Author(s):

Karen Lindsay ◽

Michael Popp ◽

Jason Norsworthy ◽

Muthukumar Bagavathiannan ◽

Stephen Powles ◽

...

Keyword(s):

Decision Support ◽

Weed Management ◽

Management Practices ◽

Palmer Amaranth ◽

Yield Potential ◽

Management Options ◽

Economic Implications ◽

Southern Us ◽

Soil Seedbank

Palmer amaranth is the most troublesome weed problem in mid-southern US crop production. Herbicides continue to be the most commonly employed method for managing Palmer amaranth, despite the weed’s widespread resistance to them. Therefore, farmers need research and extension efforts that promote the adoption of integrated weed management (IWM) techniques. Producers, crop consultants, educators, and researchers would be more likely to deploy diversified chemical and nonchemical weed management options if they are more informed about long-term biological and economic implications via user-friendly decision-support software. Described within is a recently developed software that demonstrates the effects of Palmer amaranth management practices on soil seedbank, risk of resistance evolution, and economics over a 10-year planning horizon. Aiding this objective is a point-and-click interface that provides feedback on resistance risk, yield potential, profitability, soil seedbank dynamics, and error checking of management options.

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Herbicide Resistance Management: Recent Developments and Trends

Plants ◽

10.3390/plants8060161 ◽

2019 ◽

Vol 8 (6) ◽

pp. 161 ◽

Cited By ~ 18

Author(s):

Hugh J. Beckie ◽

Michael B. Ashworth ◽

Ken C. Flower

Keyword(s):

Weed Management ◽

Crop Production ◽

Soil Seed Bank ◽

Resistance Management ◽

Seed Banks ◽

Weed Seed ◽

Recent Developments ◽

Evolution Of Resistance ◽

Sites Of Action ◽

Postemergence Herbicides

This review covers recent developments and trends in herbicide-resistant (HR) weed management in agronomic field crops. In countries where input-intensive agriculture is practiced, these developments and trends over the past decade include renewed efforts by the agrichemical industry in herbicide discovery, cultivation of crops with combined (stacked) HR traits, increasing reliance on preemergence vs. postemergence herbicides, breeding for weed-competitive crop cultivars, expansion of harvest weed seed control practices, and advances in site-specific or precision weed management. The unifying framework or strategy underlying these developments and trends is mitigation of viable weed seeds into the soil seed bank and maintaining low weed seed banks to minimize population proliferation, evolution of resistance to additional herbicidal sites of action, and spread. A key question going forward is: how much weed control is enough to consistently achieve the goal of low weed seed banks? The vision for future HR weed management programs must be sustained crop production and profitability with reduced herbicide (particularly glyphosate) dependency.

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The Pitfalls of Relating Weeds, Herbicide Use, and Crop Yield: Don't Fall Into the Trap! A Critical Review

Frontiers in Agronomy ◽

10.3389/fagro.2020.615470 ◽

2020 ◽

Vol 2 ◽

Author(s):

Nathalie Colbach ◽

Sandrine Petit ◽

Bruno Chauvel ◽

Violaine Deytieux ◽

Martin Lechenet ◽

...

Keyword(s):

Crop Yield ◽

Weed Management ◽

Crop Production ◽

Yield Loss ◽

Crop Yields ◽

Cropping Systems ◽

Crop Productivity ◽

Arable Farming ◽

Herbicide Use ◽

The Impact

The growing recognition of the environmental and health issues associated to pesticide use requires to investigate how to manage weeds with less or no herbicides in arable farming while maintaining crop productivity. The questions of weed harmfulness, herbicide efficacy, the effects of herbicide use on crop yields, and the effect of reducing herbicides on crop production have been addressed over the years but results and interpretations often appear contradictory. In this paper, we critically analyze studies that have focused on the herbicide use, weeds and crop yield nexus. We identified many inconsistencies in the published results and demonstrate that these often stem from differences in the methodologies used and in the choice of the conceptual model that links the three items. Our main findings are: (1) although our review confirms that herbicide reduction increases weed infestation if not compensated by other cultural techniques, there are many shortcomings in the different methods used to assess the impact of weeds on crop production; (2) Reducing herbicide use rarely results in increased crop yield loss due to weeds if farmers compensate low herbicide use by other efficient cultural practices; (3) There is a need for comprehensive studies describing the effect of cropping systems on crop production that explicitly include weeds and disentangle the impact of herbicides from the effect of other practices on weeds and on crop production. We propose a framework that presents all the links and feed-backs that must be considered when analyzing the herbicide-weed-crop yield nexus. We then provide a number of methodological recommendations for future studies. We conclude that, since weeds are causing yield loss, reduced herbicide use and maintained crop productivity necessarily requires a redesign of cropping systems. These new systems should include both agronomic and biodiversity-based levers acting in concert to deliver sustainable weed management.

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A rotation design to reduce weed density in organic farming

Renewable Agriculture and Food Systems ◽

10.1017/s1742170510000256 ◽

2010 ◽

Vol 25 (3) ◽

pp. 189-195 ◽

Cited By ~ 29

Author(s):

Randy L. Anderson

Keyword(s):

Population Dynamics ◽

Organic Farming ◽

Weed Management ◽

Crop Production ◽

Soil Health ◽

Population Based ◽

Weed Population ◽

Weed Density ◽

Organic Systems ◽

Herbicide Use

AbstractWeeds are a major obstacle to successful crop production in organic farming. Producers may be able to reduce inputs for weed management by designing rotations to disrupt population dynamics of weeds. Population-based management in conventional farming has reduced herbicide use by 50% because weed density declines in cropland across time. In this paper, we suggest a 9-year rotation comprised of perennial forages and annual crops that will disrupt weed population growth and reduce weed density in organic systems. Lower weed density will also improve effectiveness of weed control tactics used for an individual crop. The rotation includes 3-year intervals of no-till, which will improve both weed population management and soil health. Even though this rotation has not been field tested, it provides an example of designing rotations to disrupt population dynamics of weeds. Also, producers may gain additional benefits of higher crop yield and increased nitrogen supply with this rotation design.

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Weed Management with Glyphosate- and Glufosinate-Based Systems in PHY 485 WRF Cotton

Weed Technology ◽

10.1614/wt-d-10-00008.1 ◽

2011 ◽

Vol 25 (2) ◽

pp. 183-191 ◽

Cited By ~ 31

Author(s):

Jared R. Whitaker ◽

Alan C. York ◽

David L. Jordan ◽

A. Stanley Culpepper

Keyword(s):

Weed Control ◽

Weed Management ◽

Fiber Quality ◽

Palmer Amaranth ◽

Weed Species ◽

Crop Tolerance ◽

The North ◽

Annual Grasses ◽

Resistance Trait ◽

Cotton Belt

Glyphosate-resistant (GR) Palmer amaranth has become a serious pest in parts of the Cotton Belt. Some GR cotton cultivars also contain the WideStrike™ insect resistance trait, which confers tolerance to glufosinate. Use of glufosinate-based management systems in such cultivars could be an option for managing GR Palmer amaranth. The objective of this study was to evaluate crop tolerance and weed control with glyphosate-based and glufosinate-based systems in PHY 485 WRF cotton. The North Carolina field experiment compared glyphosate and glufosinate alone and in mixtures applied twice before four- to six-leaf cotton. Additional treatments included glyphosate and glufosinate mixed withS-metolachlor or pyrithiobac applied to one- to two-leaf cotton followed by glyphosate or glufosinate alone on four- to six-leaf cotton. All treatments received a residual lay-by application. Excellent weed control was observed from all treatments on most weed species. Glyphosate was more effective than glufosinate on glyphosate-susceptible (GS) Palmer amaranth and annual grasses, while glufosinate was more effective on GR Palmer amaranth. Annual grass and GS Palmer amaranth control by glyphosate plus glufosinate was often less than control by glyphosate alone but similar to or greater than control by glufosinate alone, while mixtures were more effective than either herbicide alone on GR Palmer amaranth. Glufosinate caused minor and transient injury to the crop, but no differences in cotton yield or fiber quality were noted. This research demonstrates glufosinate can be applied early in the season to PHY 485 WRF cotton without concern for significant adverse effects on the crop. Although glufosinate is often less effective than glyphosate on GS Palmer amaranth, GR Palmer amaranth can be controlled with well-timed applications of glufosinate. Use of glufosinate in cultivars with the WideStrike trait could fill a significant void in current weed management programs for GR Palmer amaranth in cotton.

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Differential Response of Arkansas Palmer Amaranth (Amaranthus palmeri) to Glyphosate and Mesotrione

Weed Technology ◽

10.1017/wet.2018.34 ◽

2018 ◽

Vol 32 (5) ◽

pp. 579-585 ◽

Cited By ~ 4

Author(s):

Shilpa Singh ◽

Nilda Roma-Burgos ◽

Vijay Singh ◽

Ed Allan L. Alcober ◽

Reiofeli Salas-Perez ◽

...

Keyword(s):

Dose Response ◽

Differential Response ◽

Palmer Amaranth ◽

Amaranthus Palmeri ◽

Susceptible Population ◽

Tolerance Level ◽

Crop Fields ◽

Greenhouse Study ◽

Effective Doses

AbstractWe conducted a greenhouse study to evaluate the differential response of Palmer amaranth to glyphosate and mesotrione and to quantify the level of tolerance to mesotrione in recalcitrant (difficult-to-control) accessions and their offspring. Seeds were collected from 174 crop fields (corn, cotton, and soybean) across Arkansas between 2008 and 2016. Palmer amaranth seedlings (7 to 10 cm tall) were treated with glyphosate at 840 g ae ha–1or mesotrione at 105 g ha–1. Overall, 47% of the accessions (172) were resistant to glyphosate with 68% survivors. Almost 35% of accessions were highly resistant, with 90% survivors. The majority of survivors from glyphosate application incurred between 31% and 60% injury. Mesotrione killed 66% of the accessions (174); the remaining accessions had survivors with injury ranging from 61% to 90%. Accessions with the least response to mesotrione were selected to determine tolerance level. Dose–response assays were conducted with four recalcitrant populations and their F1progeny. The average effective doses (ED50) for the parent accessions and F1progeny of survivors were 21.5 g ha–1and 27.5 g ha–1, respectively. The recalcitrant parent populations were three- to five-fold more tolerant to mesotrione than the known susceptible population, as were the F1progeny.

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Sumatran Fleabane (Erigeron sumatrensis) Resistant to PSI-Inhibitor Herbicides and Physiological Responses to Paraquat

Weed Science ◽

10.1017/wsc.2021.70 ◽

2021 ◽

pp. 1-26

Author(s):

Jéssica F. L. Leal ◽

Amanda dos S. Souza ◽

Junior Borella ◽

André Lucas S. Araujo ◽

Ana Claudia Langaro ◽

...

Keyword(s):

Dose Response ◽

Weed Management ◽

Crop Production ◽

Antioxidant Enzyme Activities ◽

Multiple Resistance ◽

Fast Recovery ◽

Continuous Growth ◽

Herbicide Resistant ◽

Paraquat Resistance ◽

Resistance To Herbicides

Abstract Herbicide-resistant weed management is one of the greatest agricultural challenges in crop production. Thus, the quick identification of resistant-herbicide weeds is extremely important for management. This study aimed to evaluate resistance to PSI-inhibitor herbicides (diquat) of Sumatran Fleabane [(Erigeron sumatrensis (Retz.) E.Walker)] and physiological response to paraquat application. The research was conducted with two E. sumatrensis biotypes, one susceptible and the other with multiple resistance to herbicides from five different modes of action (glyphosate, paraquat, diuron, saflufenacil, and 2,4-D). A dose-response assay was carried out to evaluate herbicide resistance to diquat in paraquat-resistant E. sumatrensis biotype. The enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), hydrogen peroxide (H2O2) content, and chlorophyll a fluorescence were measured in both biotypes after paraquat (400 g ai ha−1) application. The dose-response assay confirmed resistance of E. sumatrensis to diquat with resistance factor levels of 26-fold and 6-fold for LD50 and GR50 values, respectively, compared with the susceptible biotype. The accumulation of H2O2 occurred faster in the paraquat-susceptible biotype than in the resistant ones. Paraquat treatment caused an increase in SOD and APX activity in the susceptible biotype, but antioxidant enzyme activities were unaffected by paraquat in the resistant one at 5 hours after application (HAA). Chlorophyll a fluorescence increased along the first 4 HAA in both resistant and susceptible biotypes. However, at 24 HAA the resistant biotype showed a decline in fluorescence close to untreated plants while susceptible one died, which can be used to diagnose paraquat resistance at 24 HAA. There is confirmed resistance to diquat in a paraquat-resistant E. sumatrensis biotype. The paraquat-resistant biotype does not induce antioxidative enzymes, as a possible mechanism of resistance to paraquat, but shows a fast recovery of photosynthesis and continuous growth when subjected to paraquat, while the paraquat-susceptible biotype does not survive.

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Effects of Palmer Amaranth (Amaranthus palmeri) Establishment Time and Distance from the Crop Row on Biological and Phenological Characteristics of the Weed: Implications on Soybean Yield

Weed Science ◽

10.1017/wsc.2018.84 ◽

2019 ◽

Vol 67 (1) ◽

pp. 126-135 ◽

Cited By ~ 8

Author(s):

Nicholas E. Korres ◽

Jason K. Norsworthy ◽

Andy Mauromoustakos

Keyword(s):

Seed Production ◽

Weed Management ◽

Cropping Systems ◽

Management Strategies ◽

Palmer Amaranth ◽

Yield Reduction ◽

Amaranthus Palmeri ◽

Soybean Yield ◽

Management Measures ◽

Weed Population Dynamics

AbstractInformation about weed biology and weed population dynamics is critical for the development of efficient weed management programs. A field experiment was conducted in Fayetteville, AR, during 2014 and 2015 to examine the effects of Palmer amaranth (Amaranthus palmeriS. Watson) establishment time in relation to soybean [Glycine max(L.) Merr.] emergence and the effects ofA. palmeridistance from the soybean row on the weed’s height, biomass, seed production, and flowering time and on soybean yield. The establishment time factor, in weeks after crop emergence (WAE), was composed of six treatment levels (0, 1, 2, 4, 6, and 8 WAE), whereas the distance from the crop consisted of three treatment levels (0, 24, and 48 cm). Differences inA. palmeribiomass and seed production averaged across distance from the crop were found at 0 and 1 WAE in both years. Establishment time had a significant effect onA. palmeriseed production through greater biomass production and height increases at earlier dates.Amaranthus palmerithat was established with the crop (0 WAE) overtopped soybean at about 7 and 10 WAE in 2014 and 2015, respectively. Distance from the crop affectedA. palmeriheight, biomass, and seed production. The greater the distance from the crop, the higherA. palmeriheight, biomass, and seed production at 0 and 1 WAE compared with other dates (i.e., 2, 4, 6, and 8 WAE).Amaranthus palmeriestablishment time had a significant impact on soybean yield, but distance from the crop did not. The earlierA. palmeriinterfered with soybean (0 and 1 WAE), the greater the crop yield reduction; after that period no significant yield reductions were recorded compared with the rest of the weed establishment times. Knowledge ofA. palmeriresponse, especially at early stages of its life cycle, is important for designing efficient weed management strategies and cropping systems that can enhance crop competitiveness. Control ofA. palmeriwithin the first week after crop emergence or reduced distance between crop and weed are important factors for an effective implementation of weed management measures againstA. palmeriand reduced soybean yield losses due to weed interference.

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