scholarly journals Physiological and Molecular Investigation of Urea Uptake Dynamics in Cucumis sativus L. Plants Fertilized With Urea-Doped Amorphous Calcium Phosphate Nanoparticles

2021 ◽  
Vol 12 ◽  
Author(s):  
Sebastian B. Feil ◽  
Giacomo Rodegher ◽  
Federica Gaiotti ◽  
Monica Yorlady Alzate Zuluaga ◽  
Francisco J. Carmona ◽  
...  

At present, the quest for innovative and sustainable fertilization approaches aiming to improve agricultural productivity represents one of the major challenges for research. In this context, nanoparticle-based fertilizers can indeed offer an interesting alternative with respect to traditional bulk fertilizers. Several pieces of evidence have already addressed the effectiveness of amorphous calcium phosphate-based nanoparticles as carriers for macronutrients, such as nitrogen (N), demonstrating increase in crop productivity and improvement in quality. Nevertheless, despite N being a fundamental nutrient for crop growth and productivity, very little research has been carried out to understand the physiological and molecular mechanisms underpinning N-based fertilizers supplied to plants via nanocarriers. For these reasons, this study aimed to investigate the responses of Cucumis sativus L. to amorphous calcium phosphate nanoparticles doped with urea (U-ACP). Urea uptake dynamics at root level have been investigated by monitoring both the urea acquisition rates and the modulation of urea transporter CsDUR3, whereas growth parameters, the accumulation of N in both root and shoots, and the general ionomic profile of both tissues have been determined to assess the potentiality of U-ACP as innovative fertilizers. The slow release of urea from nanoparticles and/or their chemical composition contributed to the upregulation of the urea uptake system for a longer period (up to 24 h after treatment) as compared to plants treated with bulk urea. This prolonged activation was mirrored by a higher accumulation of N in nanoparticle-treated plants (approximately threefold increase in the shoot of NP-treated plants compared to controls), even when the concentration of urea conveyed through nanoparticles was halved. In addition, besides impacting N nutrition, U-ACP also enhanced Ca and P concentration in cucumber tissues, thus having possible effects on plant growth and yield, and on the nutritional value of agricultural products.

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Michele Iafisco ◽  
Lorenzo Degli Esposti ◽  
Gloria Belén Ramírez-Rodríguez ◽  
Francesca Carella ◽  
Jaime Gómez-Morales ◽  
...  

2018 ◽  
Vol 10 (2) ◽  
pp. 11-29
Author(s):  
Wahyu Wardiana Dewi

This research is aimet to known the responsed of cucumber plant (Cucumis sativus L.), the good means for the growth and yield of cucumber (Cucumis sativus L) of the hybrid varieties doses of good means. This study uses a randomized block design (RAK) with one factor and 5 replications. The main factors, namely: goat manure. First repeat that: without fertilizer (P0), manure goat 10 ton / ha (P1), manure goat 20 ton / ha (P2), manure goat 30 ton / ha (P3), and the latter is manure goat 40 ton / ha (P4). The parameters observed were plant height, leaf number, fruit weight, fruit length and diameter of the fruit. Data analysis by using analysis of variance (ANOVA), if the effect followed by Least Significant Difference Test (BNT). The results based on the analysis of variance showed that: 1) Treatment of manure goat (P) significantly affected the growth and yield of cucumber plants include all the parameters of observation starting plant height, leaf number, fruit weight, fruit length and fruit diameter. Dose goat manure 40 t / ha is the best concentration to produce the highest average values for all parameters. The lowest value of all parameters are shown on the dose P0 (without manure goat / control).


2005 ◽  
Vol 873 ◽  
Author(s):  
Balasundaram G ◽  
Sato M ◽  
Webster TJ

AbstractIn an effort to decrease the number of problems associated with osteoporosis, the long-term goal of the present study is to design calcium phosphate-based nanoparticles that specifically attach to areas of low bone density and once attached, allow for the targeted release of bioactive agents that can quickly increase bone formation. Efforts are focused on nanoparticles of calcium phosphate-based materials since they are similar in size and chemistry to the major inorganic components of bone. As a first step in this research, the objective of the present study was to synthesize nanoparticles of crystalline hydroxyapatite (or HA) and amorphous calcium phosphate. Crystalline HA is stable under physiological fluids and, thus, will release embedded bioactive agents slowly. Alternatively, amorphous calcium phosphate is highly biodegradable and will, thus, release embedded bioactive agents quickly. A further objective of the present study was to functionalize such inorganic biodegradable materials with amino groups which would allow for the subsequent attachment of entities to direct such nanoparticles to osteoporotic bone and increased bone formation once attached. One promising approach to direct the nanoparticles to osteoporotic bone is to attach antibodies to pentosidine on the nanoparticles since pentosidine is present in higher amounts in osteoporotic compared to healthy bone. A promising approach to increase bone growth once nanoparticles attach to osteoporotic bone, is to embed nanoparticles with regions of the bone growth factor: bone morphogenic protein-2 (or BMP-2). Results of this study demonstrated the successful synthesis of both crystalline HA and amorphous calcium phosphate nanoparticles. Furthermore, results showed that these nanoparticles can be functionalized with versatile amino groups. In this manner, this study takes the first steps toward utilizing calcium phosphate based nanoparticles to reverse bone loss associated with osteoporosis.


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