The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings

2007 ◽  
Vol 87 (1) ◽  
pp. 49-57 ◽  
Author(s):  
J. Chen ◽  
C. Zhu ◽  
D. Lin ◽  
Z. -X Sun
Keyword(s):  
Lipid Peroxidation ◽  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Wild Type ◽  
Rice Seedlings ◽  
Sensitive Mutant ◽  
Cd Toxicity ◽  
Potential Biomarker

Cadmium-sensitive rice (Oryza sativa L. subsp. Japonica ‘Zhonghua11’) mutants were obtained using an Agrobacterium tumefaciens-based gene delivery system. Significant phenotypic differences were observed between a Cd-sensitive mutant (ST) and wild type (WT) rice seedlings. Results indicated that Cd accumulation in the leaves of the mutant was twice that of the wild type after 10 d of 0.5 mM Cd2+ treatment. Furthermore, a rapid Cd-induced H2O2 increase was observed in the mutant leaves, which induced abnormally early activity in antioxidant enzymes such as superoxide dismutase (SOD). However, the mutant leaves showed lower catalase (CAT) activity. By contrast, guaiacol peroxidase (G-POD) activities were higher in the mutant than in the wild type roots. Together with the Cd toxicity-induced decline of early responsive enzymatic activities in vivo, especially CAT, the inability of mutants to scavenge accumulated H2O2 resulted in higher lipid peroxide levels. H2O2 might also strengthen the expression of G-POD as a signaling molecule. Results suggest that G-POD activity can be a potential biomarker reflecting Cd sensitivity in rice seedlings. Key words: Antioxidant enzyme, Cd toxicity, Cd-sensitive mutant, lipid peroxidation, rice (Oryza sativa L.)

Cadmium-induced changes in antioxidant enzyme activities in rice (Oryza sativa L. cv. Dongjin)

2002 ◽  
Vol 45 (3) ◽  
pp. 134-140 ◽  
Author(s):  
Mohammad Babar Ali ◽  
Hyun Sik Chun ◽  
Byung Ki Kim ◽  
Chin Bum Lee
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Induced Changes

scholarly journals Seed priming with hydrogen peroxide alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings

2020 ◽  
Vol 48 (1) ◽  
pp. 273-283
Author(s):  
Weeraphorn JIRA-ANUNKUL ◽  
Wattana PATTANAGUL
Keyword(s):  
Hydrogen Peroxide ◽  
Oryza Sativa ◽  
Drought Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Rice Seedling ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase

Drought stress is a major factor limiting crop growth and yield. Hydrogen peroxide (H2O2) is known as a signalling molecule in the plant cell in which activates multiple physiological changes that play essential roles in tolerance mechanism. This study investigated the effects of seed priming with H2O2 on growth, some physiological characteristics and antioxidant enzyme activities in rice seedling under drought stress. Rice (Oryza sativa L.) cv. Khao Dawk Mali 105 seeds were primed with 0 (distilled water), 1, 5, 10, and 15 mM H2O2 and grown for 21 days. The seedlings were subjected to drought stress by withholding water for 7 days. The results showed that priming with low concentrations of H2O2 improved plant growth and biomass as well as relative water content, malondialdehyde content, electrolyte leakage. Priming with H2O2, however, had no beneficial effect on chlorophyll content, proline and leaf total soluble sugar. Seed priming with appropriate levels of H2O2 also enhanced antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). It is concluded that seed priming with 2-10 mM H2O2, is beneficial for enhancing drought tolerance in rice seedling by increasing antioxidant capacity, which in turn reduces oxidative stress and damages to the cellular components.

scholarly journals Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Core Protein ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Electron Flow ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Transcription Analysis ◽  
Chl A ◽  
Chl Biosynthesis

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

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