scholarly journals Effects of Intrauterine Infusion of a Chitosan Solution on Recovery and Subsequent Reproductive Performance of Early Postpartum Dairy Cows with Endometritis: A Pilot Field Trial

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 197
Author(s):  
Hiroaki Okawa ◽  
Missaka M.P. Wijayagunawardane ◽  
Peter L.A.M. Vos ◽  
Osamu Yamato ◽  
Masayasu Taniguchi ◽  
...  

This study investigated the efficacy of intrauterine infusion of a chitosan solution (CHT) on uterine recovery in early postpartum dairy cows with or without endometritis, and their subsequent reproductive performance. In Experiment 1, cows with endometritis at 3 weeks postpartum were administered CHT (n = 5) and prostaglandin F2α (PGF2α) (n = 4). Untreated cows (n = 7) served as the control group. In Experiment 2, 18 cows with a normally recovered uterus at the fresh cow check (mean, 35 days postpartum) were assigned to the CHT (n = 10) and control (n = 8) groups, and intrauterine infusion was conducted in the CHT group. Overall, in Experiment 1, the percentage of polymorphonuclear leukocytes significantly declined in the CHT group (32.3 ± 10.2 to 5.5 ± 2.4, p < 0.05) from week 3 to week 5, but no decline occurred in the PGF2α and control groups. In Experiment 2, the CHT and control groups showed no significant differences in reproductive parameters, suggesting the absence of adverse effects of CHT on fertility. These results suggest that intrauterine infusion of CHT in the early postpartum period effectively accelerates uterine recovery from endometritis and might be a suitable replacement for PGF2α administration.

1990 ◽  
Vol 78 (03) ◽  
pp. 132-134 ◽  
Author(s):  
A.V. Williamson ◽  
W.J. Crawford ◽  
B. Rennie ◽  
W.L. Mackie

AbstractOverall reproductive performance and associated periparturient disorders were monitored in a herd of British Friesian cross dairy cows from September 1987 to August 1988. Pregnancy diagnosis and cyclical status of the herd was determined by palpation of the ovaries per rectum. A total of 49 (110) cows were randomly treated with Sepia 200c either on day 14(19) or 21 days (30) postpartum.Statistical analysis of the results was based on the differences between the control and combined Sepia-treated groups. Although there were significant differences in the proportion of heifers calving in each group, there was no significant difference in periparturient disorders or during the pre-service period.However, in the Sepia Group that held a lower proportion of heifers, a significant difference was found in the conception rate to 1st service (47%), the percentage of cows in calf (90%) and total culling rate (10%) compared to the Control Group (26%, 72% and 28% respectively). A reduction (non significant) was found between the Sepia and Control services to conception (0.2), calving to conception interval (5 days) and calving index (2 days).The results of this pilot study appear to demonstrate a difference in effect between the Sepia-treated Group and Control Group. The study has been extended to treat a larger number of dairy cows to examine the reproducibility of these results.


2009 ◽  
Vol 21 (1) ◽  
pp. 245
Author(s):  
K. Oshima ◽  
Y. Ochiai ◽  
T. Kojima ◽  
N. Yamamoto

The objective of this study was to investigate the effect of GnRH and EB injection on the SOV response in early postpartum suckling Japanese Black cows. Forty-eight cows were included in this study. All cows received a controlled internal-drug-releasing device containing 1.9 g progesterone (CIDR; Easi-Breed; InterAg, Hamilton, New Zealand) at 40 days postpartum, and were subsequently superstimulated with a total dose of 20 armour units FSH (Antrin 40; Kawasaki-Mitaka, Kanagawa, Japan) twice daily, with gradually decreasing doses from day 45 till day 47. CIDR was withdrawn with 0.75 mg of cloprostenol (Estrumate; Nagase Medicals, Itami, Japan) injection at day 47. After FSH treatment, the cows were assigned to 3 groups: (1) 100 μg GnRH (Conceral; Nagase Medicals) injection at day 49 (GnRH, n = 19), (2) 2 mg EB (Kawasaki-Mitaka) injection at day 48 (EB, n = 15), and (3) control (n = 14). AI was carried out twice based on the estrous behavior of the cows, which was examined several times daily. Embryos were nonsurgically collected 7 to 8 days after estrus. The ovaries were examined by ultrasonography, and the number of CL and unovulated follicles (UF) were counted. After uterine flushing, the cows were re-employed for reproductive purposes. The intervals to first estrus after flushing and days open were examined. Blood samples were collected temporally, and concentrations of progesterone (P4) and estradiol (E2) were measured. The ovaries were examined by ultrasonography and the number of CL, small follicles (<5 mm in diameter), medium follicles (between 5 and 10 mm in diameter), and large follicles (>10 mm in diameter) were counted temporally. Data were analyzed by GLM and Dunnett’s T3 test. There were no significant differences between both treatment groups and the control group in the number of CL (15.2 ± 6.8, 18.9 ± 9.4, and 14.9 ± 8.9 in the GnRH, EB, and control groups, respectively; mean ± SD), UF (5.3 ± 3.2, 4.7 ± 3.2, and 7.2 ± 9.5, respectively), recovered ova or embryos (11.4 ± 8.1, 11.1 ± 8.9, and 11.1 ± 9.1, respectively), transferable embryos (6.8 ± 5.3, 7.5 ± 7.6, 7.2 ± 6.7, respectively), and freezable embryos (6.3 ± 5.6, 6.6 ± 7.9, and 5.7 ± 6.1, respectively). Furthermore, there were no significant differences in the intervals to first estrus after flushing and days open (12.7 ± 13.6 and 90.0 ± 23.1 in the GnRH, 8.6 ± 5.3 and 79.1 ± 20.1 in the EB, and 9.4 ± 6.0 and 88.5 ± 33.8 in the control groups, respectively). Changes in the P4 level were similar among the 3 groups. The E2 level of the EB group at 15 h after EB injection was significantly higher (P < 0.05) than that in the other groups (19.0 ± 15.7, 49.4 ± 22.8, and 8.0 ± 5.7 pg mL–1 in the GnRH, EB, and control groups, respectively). The number of small follicles in the control group (17.0 ± 12.2) was significantly greater (P < 0.05) than that in the GnRH group (7.4 ± 7.2) at the first AI. These results suggest that EB and GnRH injection after SOV treatment in early postpartum Japanese Black cows does not affect the SOV response or embryo quality.


2013 ◽  
Vol 25 (1) ◽  
pp. 154
Author(s):  
J. K. Jeong ◽  
H. G. Kang ◽  
T. Y. Hur ◽  
I. H. Kim

Early postpartum cyclicity is related to subsequent fertility. This field study evaluated the effect of gonadotropin-releasing hormone (GnRH) administration around the first postpartum month on subsequent reproductive performance in dairy cows. Holstein dairy cows underwent ultrasonography at 30.0 (±6) days postpartum to determine the existence of ovarian structures including corpus lutea (CL), follicles, or cysts. The cows without CL were divided randomly into 2 treatment groups: GnRH group and control group. Cows in the GnRH group received an injection of 100 µg of GnRH (gonadorelin, Godorel, Uni-Biotech Co. Ltd., Korea; n = 151). Cows in the control group received no GnRH (n = 171). Cows diagnosed with endometritis at the time of ultrasonography were excluded from this study. Cows received the normal herd reproductive management program including estrous detection or synchronization, or synchronization of ovulation in the breeding period. The voluntary waiting period from calving to first AI in this study was 40 days. Pregnancy was determined per rectum 60 days after AI by both ultrasonography and palpation. Reproductive performance data were collected for a minimum of 210 days postpartum. Reproduction data between the GnRH and control groups were evaluated by chi-square test or the Cox proportional hazard model with the PHREG procedure using the SAS program (version 9.1, SAS Institute Inc., Cary, NC, USA). The number of days to first insemination after calving did not differ (P > 0.05) between the control group (80.9 ± 2.2 days) and the GnRH group (82.1 ± 2.3 days), while the pregnancy rate to first insemination tended to be higher (P = 0.1) in the GnRH group (42.3%) than in the control group (33.3%). Furthermore, the pregnancy hazard was higher by 210 days in milk for the GnRH group compared with the control group (hazard ratio = 1.33; P < 0.05), which resulted in a 26-day reduction in the median interval to pregnancy. In conclusion, the administration of GnRH around the first postpartum month improved subsequent reproductive performance in dairy cows. This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (project no. PJ008464) Rural Development Administration, Republic of Korea.


2021 ◽  
Author(s):  
Massoud - Talebkhan Garoussi ◽  
Omid Mavadati ◽  
Mohammad Ali Bahonar ◽  
Mehran Dabiri

Abstract Sheep are considered short-day breeders. Exposure of rams to anestrus ewes results in an increase in LH secretion. The aims of this study was to determine whether the presence and exposure of rams can effect on reproductive performance such as: pregnancy rate, litter size, sex of lamb, fecundity rate and prolificacy rate of fat tail Iranian Shaal breed ewes in non-breeding season using progesterone (p4) analogue with or without equine chorionic gonadotropin (eCG). Totally, 100 ewes out of 138 non-cyclic ewes with <0.5ng/ml P4 were selected for this study. They were aged 2 to >7 years old. They were stratified to two groups (control and treatment). The ewes in treatment and control groups received 13 days a sponge containing 60 mg medroxyprogesterone acetate (MPA). The treatment group consists of 3 subgroups and each group contains 25 ewes equally. They were included: 1- P4, eCG and Ram exposure. 2- P4 and ram exposure. 3- P4 and eCG. Ewes in control group received only P4. The ewes were located in isolated corrals for 40 days. The ram to ewe ratio was 1 to 5. Twenty healthy rams were housed in an isolated pen from ewes with distance 1500 m during 40 days. Rams exposed three days before sponge withdraw (day 10). They were separated by considerable distance (2 meters) using fence. The related groups received 500 IU eCG on the day of sponge removal (day 13). The rams were released into the ewe flock after removing the sponge in treatment and control groups. Pregnancy diagnosis performed using transabdominal ultrasonography. The obtained data analyzed using SPSS version 16. The conception rate in treatment and control groups were differed significantly (P<0.01). The conception rate in P4+ram exposure+ eCG, P4+ramexposure and P4+ eCG were 96%(no.24), 80%(no.20) and 88%(no.22), respectively. However, the conception rate in control group was 60% (no. 15). The fecundity rates were 136%, 100% and 124% in treatment subgroups, however, it was 84% in control group, respectively. There were no significant differences among genus and weight of lambs in treatments subgroups and control group (P>0.05). It is concluded that ram exposure can increase significantly reproductive performance in non-breeding season fat tailed ewes Shaal breed.


2012 ◽  
Vol 58 (3) ◽  
pp. 366-370 ◽  
Author(s):  
Pilar SANTOLARIA ◽  
Fernando L^|^Oacute;PEZ-GATIUS ◽  
Jos^|^eacute; Antonio S^|^Aacute;NCHEZ-NADAL ◽  
Jes^|^uacute;s Y^|^Aacute;NIZ

2014 ◽  
Vol 26 (1) ◽  
pp. 118
Author(s):  
M. Orozco ◽  
C. G. Gutierrez ◽  
R. Lopez ◽  
C. Aguilar ◽  
C. Roque ◽  
...  

The present study tested whether the increase in serum progesterone concentrations for 6 days before oestrus synchronization with prostaglandin F2α (PGF2) increases oestral response, pregnancy rate, and reduces the incidence of twin births in dairy cattle. Seven hundred and eighty-three first-service dairy cows were synchronized with 2 injections of PGF2 14 days apart, starting on Day 35 postpartum. Six days before the second PGF2 injection, cows were assigned to 2 groups: P4 and control. The P4 group (n = 387) received a progesterone-releasing intravaginal device and an intramuscular injection of 500 mg of progesterone. The control group (n = 396) did not receive the progesterone-releasing intravaginal device or progesterone injection. Cows were inseminated 12 h after exhibiting oestrus. Pregnancy diagnosis was performed 40 and 45 days after insemination by rectal palpation and the cumulative pregnancy rate on Day 90 postpartum was determined retrospectively through the individual records. The response to the progesterone treatment varied according to the parity of the cows (P < 0.05). Thus, progesterone administration improved the percentage of cows detected in oestrus in multiparous [192/255 (75.2%) v. 161/267 (60.2%)] but not in primiparous [93/132 (70.4%) v. 90/129 (69.7%)] cows. Progesterone treatment increased pregnancy rate in multiparous [53/192 (27.6%) v. 27/161 (16.7%)] but not in primiparous cows [25/93 (26.8%) v. 29/90 (32.2%)]. Likewise, progesterone administration increased the cumulative percentage of pregnant cows on day 90 postpartum in multiparous [149/255 (58.4%) v. 129/267 (48.3%)] but not in primiparous cows [77/132 (58.3%) v. 82/129 (63.5%)]. The incidence of twin births was lower (P = 0.07) in cows treated with progesterone [1/74 (1%)] than in the control group [4/53 (7%)]. It is concluded that progesterone administration before oestrus synchronization with PGF2 in first service dairy cows improves oestral response, increases pregnancy rate, and percentage of pregnant animals on Day 90 postpartum in multiparous cows, but not in primiparous cows, and decreases the incidence of twin births. This research was supported by PAPIIT-UNAM research grant (IN219811-3).


2021 ◽  
Vol 71 (9) ◽  
pp. 2124-2129
Author(s):  
Sevda Korkut Oksuz ◽  
Sevil Inal

Objective: This study aims to determine the effect of kangaroo mother care applied to the healthy newborns in the early postpartum period on breastfeeding. Methods:  Our research was conducted over the period June 1 - August 25, 2016 at the obstetrics and maternity units in Istanbul. A of 112 healthy newborns and their mothers (56 in the study group and 56 in the control group) were recruited into a randomized controlled experimental study. The newborns in the study group were administered kangaroo mother care for 3 hours after birth while the control group of newborns was cared for using the unit’s standard postpartum procedures. The time the newborns in the study and control groups first started to breastfeed, the number of breastfeeding within the first 24 hours, the duration of their breastfeeding and suckling skills were evaluated. Results:  It was found that newborns subjected to kangaroo mother care provided immediately after delivery started suckling at the breast sooner (P = .001), for a longer period (P = .001) and more frequently (P = .001) than the newborns provided with standard post-delivery care. While there was no difference between the groups in terms of initial suckling skills (P = .862), the newborns in the kangaroo mother care group were better at suckling in the 24th hour (P = .001). Continuous...


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