scholarly journals A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of Takifugu rubripes

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3419
Author(s):  
He Zhou ◽  
Yuqing Sun ◽  
Xin Li ◽  
Ziyu Zhou ◽  
Kexin Ma ◽  
...  
Keyword(s):  
Low Temperature ◽  
Transcriptome Sequencing ◽  
Average Length ◽  
Gonadal Development ◽  
Temperature Treatment ◽  
Takifugu Rubripes ◽  
Rna Seq ◽  
Low Temperature Treatment ◽  
Cultured Fish ◽  
Insight Into

The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of Takifugu rubripes, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of T. rubripes (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (LOC101066759, dgat1, limk1, fbxl3, col6a3, fgfr3, dusp22b, svil, abhd17b, srgap3, tmem88b, bud4, and mustn10) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for T. rubripes and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.

scholarly journals Transcriptome Analysis of Low-Temperature-Induced Breaking of Garlic Aerial Bulb Dormancy

2019 ◽  
Vol 2019 ◽  
pp. 1-16
Author(s):  
Yuhui Dong ◽  
Mengjiao Guan ◽  
Lixia Wang ◽  
Lei Yuan ◽  
Xiudong Sun ◽  
...  
Keyword(s):  
Low Temperature ◽  
Calcium Signaling ◽  
High Throughput ◽  
Theoretical Basis ◽  
Transcriptome Sequencing ◽  
Temperature Treatment ◽  
Accessory Pathways ◽  
Low Temperature Treatment ◽  
Bulb Dormancy ◽  
First Choice

The long history of asexual reproduction of garlic using garlic cloves has resulted in virus accumulation and genetic depression. Propagation of garlic seedlings by tissue culture can both eliminate viruses and improve breeding efficiency. Aerial bulbs are the first-choice materials for breeding virus-free garlic seedlings under external conditions, but they show dormancy just like garlic bulbs. However, low temperatures can quickly break dormancy. In this research, we used a high-throughput sequencing method to sequence aerial bulbs during dormancy and after low-temperature-induced breaking of dormancy to screen out the key differentially expressed genes (DEGs) associated with low temperature and to provide a theoretical basis for exploring the molecular mechanism of low-temperature-induced breaking of aerial bulb dormancy. The high-throughput transcriptome sequencing results showed that 6,675 DEGs were upregulated and 36,596 DEGs were downregulated in the aerial bulbs given low-temperature treatment. Then, 19,507 DEGs were assigned KEGG annotations, among which most DEGs were annotated to the metabolism pathway (11,817 genes, accounting for 60.58%), followed by the genetic information processing pathway (4,521 genes, accounting for 23.18%). The DEGs were mostly concentrated in pathways such as protein processing in the endoplasmic reticulum, plant-pathogen interaction, plant hormone signal transduction, and ribosome biogenesis in eukaryotes, with significant enrichment. The key DEGs related to calcium signaling, hormonal signaling, and transcription factors were screened out, including CaM, CDPK, and CML in accessory pathways of calcium signaling; GA20ox, GAI1, and GA2ox in accessory pathways of hormonal signaling; and transcription factor genes such as MYB, AP2/ERF, bHLH, MADS, and bZIP. qRT-PCR verification results were consistent with the sequencing results, indicating that the transcriptome sequencing data were accurate and reliable. Our results provide a theoretical basis for breaking the dormancy of aerial bulbs with low-temperature treatment to produce virus-free seedlings and increase the output and quality of garlic.

Studies on Freshwater Osmoregulation in the Ammocoete larva of Lampetra Planeri (Bloch)

1968 ◽  
Vol 48 (3) ◽  
pp. 597-609
Author(s):  
R. MORRIS ◽  
J. M. BULL
Keyword(s):  
Low Temperature ◽  
Extracellular Space ◽  
Ringer Solution ◽  
Temperature Treatment ◽  
Sodium Influx ◽  
Sodium Chloride Solutions ◽  
Low Temperature Treatment ◽  
Lampetra Planeri ◽  
Equilibrium Concentrations ◽  
Sodium Loss

1. An investigation has been made of the factors which cause sodium loss from ammocoetes when they are immersed in de-ionized water at 1° and 10° C. 2. Sodium influx ceases when animals are first immersed in de-ionized water, but can recommence when the animal loses sufficient sodium to the environment. The concentration of sodium required for influx to take place decreases with succeeding periods of immersion in de-ionized water at 10° C. and reaches minimum equilibrium concentrations as low as 0.005 mM-Na/l. 3. Low temperature inhibits sodium influx and thus promotes net loss of sodium to de-ionized water. 4. Low temperature also decreases the initial loss of sodium to de-ionized water and probably lowers the permeability of the external surfaces of the animal to ions. This effect is small compared with the inhibition of ion uptake so that the combined result is to increase the net loss of sodium from the animal. 5. Since animals lose calcium to de-ionized water and show a decreased rate of sodium loss when calcium salts are added, it is believed that the high rates of sodium loss in de-ionized water are attributable to the effect of calcium on permeability. 6. Lack of calcium may also explain why animals which have been depleted of sodium by low-temperature treatment take up sodium much faster at higher temperatures from dilute Ringer solutions than from pure sodium chloride solutions. 7. When animals lose ions to de-ionized water at low temperature, sodium and chloride are lost from the extracellular space, whilst the muscle cells lose potassium. These ions are recovered into the extracellular space when animals are allowed to take up ions at 10° C. from diluted Ringer solution later.

scholarly journals Development of technology of preparation of pork semi-products with the application of low-temperature treatment

2019 ◽  
Vol 81 (2) ◽  
pp. 250-256
Author(s):  
M. F. Khairullin ◽  
E. A. Koval ◽  
I. Y. Levitskaya ◽  
M. G. Gadjiev ◽  
B. A. Sultonov
Keyword(s):  
Low Temperature ◽  
Temperature Treatment ◽  
Production Costs ◽  
Meat Industry ◽  
Pork Products ◽  
Low Temperature Treatment ◽  
Organoleptic Characteristics ◽  
Microbiological Characteristics ◽  
Manufactured Products ◽  
Low Temperature Processing

The study was aimed at developing a technology for preparing various semi-finished products from pork meat using low-temperature processing "Su-Vid". The main physicochemical indicators, functional-technological and structural-mechanical characteristics of semi-finished products, the chemical composition of semi-finished products and finished products were identified and determined experimentally, and organoleptic analysis was carried out. The data indicate that the use of low-temperature processing significantly affects the characteristics of manufactured products, which improves its performance. In particular, pork semi-finished products prepared at low temperatures are characterized by the yield: steak – 93.9%, ribs – 92.6%, in comparison with the control regime, 7.5 and 6.2% higher. The complex of data obtained showed that the samples of semi-finished pork, according to the experimental regimes of heat treatment, in terms of safety, fully meet the requirements of TR TS 034/2013. Analysis of semi-finished pork products showed that products prepared using the Su-Vid technology had high organoleptic characteristics, taste and sensory characteristics. During the storage of semi-finished products, minor changes in proteins and fats, as well as organoleptic, sanitary and microbiological characteristics, took place. As a result, a bone steak was developed in the Caucasian marinade and pork ribs in the Monte Ritz marinade made using the Su-Vid technology. The results allow us to judge that this technology is quite promising and has a positive effect on the characteristics of manufactured products. The widespread introduction of this technology in the meat industry will allow the development of semi-finished products and products of high quality, nutritional and biological value compared to products manufactured by traditional methods, as well as reduce production costs.

scholarly journals LEVELS OF HORMONES TRIIODOTHYRONINE AND PLASMA BLOOD GLUCOSE BROILER STARTER DUE TO HEAT STRESS AND PRELIMINARY DIFFERENCE FEEDING TIME

2018 ◽  
Vol 6 (1) ◽  
pp. 421-427
Author(s):  
Liborio Ximenes ◽  
Pratiwi Trisunuwati ◽  
Muharlien
Keyword(s):  
Heat Stress ◽  
Blood Glucose ◽  
High Temperature ◽  
Low Temperature ◽  
Block Design ◽  
Temperature Treatment ◽  
Initial Time ◽  
Feeding Time ◽  
Glucose Levels ◽  
Low Temperature Treatment

The study was conducted to know blood glucose and triiodothyronine levels of broiler starter due to heat stress and different initial-time feeding. The material used in this study was 64 male DOC broilers of Lohmann strain with the average initial weight of 47.98 ± 2.24 g. The experiment was designed based on randomized block design with a 2x2 factorial and four replications. The treatment combinations as follow: S1W1 = low temperature, feeding 12 hours post-hatching; S1W2 = low temperature, feeding 24 hours post-hatching; S2W1 = high temperature, feeding 12 hours post-hatching; S2W2 = high temperature, feeding 24 hours post-hatching. The Data were Analyzed by using ANOVA. If there was a significant effect (P <0.05) or very significant effect (P <0.01) in Followed by Duncan's test. The result Showed that heat stress treatment significantly affected (P <0.05) to the triiodothyronine level, while initial feeding treatment had no significant effect. Also, blood glucose levels had no significant effect due to the treatments. It concluded that heat stress negatively affected by the blood glucose and triiodothyronine levels of broiler starter. The initial-time feeding has no effect on the blood glucose and triiodothyronine levels. Interactions between treatment and different temperature-time initial feeding have no effect. Nevertheless, the low temperature treatment and initial feeding of 12 hours post-hatching shows the best results with blood glucose 246.25 ± 9.91 mg / dl and triiodothyronine of 2.05 ± 0.14 ng / ml.

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