scholarly journals Age-Dependent Expression of MyHC Isoforms and Lipid Metabolism-Related Genes in the Longissimus Dorsi Muscle of Wild and Domestic Pigs

Animals ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 10 ◽  
Author(s):  
Milka Vrecl ◽  
Marko Cotman ◽  
Matjaž Uršič ◽  
Marjeta Čandek-Potokar ◽  
Gregor Fazarinc
Keyword(s):  
Lipid Metabolism ◽  
Quantitative Polymerase Chain Reaction ◽  
Longissimus Dorsi ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Domestic Pigs ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Myhc Isoforms ◽  
Age Dependent

This study aimed to compare age-dependent changes in the relative expression of genes encoding myosin heavy chain (MyHC) isoforms and selected lipid metabolism-related genes in the longissimus dorsi muscle of wild pigs (WPs) and domestic pigs (DPs). Muscles sampled from postnatal day one as well as three-week-old and two-year-old animals were used in quantitative polymerase chain reaction (qPCR) assays, histological evaluations of succinate dehydrogenase (SDH) activity, and intra-myofiber lipid (IMFL) assessment. Expression of the MyHC isoforms displayed the most extensive age- and breed-dependent changes within the first three postnatal weeks. The MyHCembry level decreased significantly faster in the WPs than in the DPs. The relative MyHC-I and -IIa expression was significantly higher in the WPs, and MyHC-IIb was substantially higher in the DPs. The differences in MyHC expression corroborated the number of SDH-positive myofibers and IMFLs. Expression of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) genes displayed only age-related variations. In summary, the evidence is provided for accelerated postnatal myofiber transformation directed towards oxidative myofibers in WPs. The SDH activity/staining intensity largely reflected the expression of MyHCs, and not genes involved in lipid uptake and utilization.

P3021 Effects of diet on the expression of lipid metabolism signaling genes in the longissimus dorsi muscle of Polish Holstein bulls

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 62-62
Author(s):  
K. Rutkowska ◽  
D. Reczynska ◽  
M. Lukaszewicz ◽  
E. Bagnicka ◽  
J. Oprzadek
Keyword(s):  
Lipid Metabolism ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Holstein Bulls

scholarly journals Identification of eQTLs associated with lipid metabolism in Longissimus dorsi muscle of pigs with different genetic backgrounds

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lourdes Criado-Mesas ◽  
Maria Ballester ◽  
Daniel Crespo-Piazuelo ◽  
Anna Castelló ◽  
Ana I. Fernández ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle

scholarly journals Dietary Ferulic Acid Supplementation Improves Antioxidant Capacity and Lipid Metabolism in Weaned Piglets

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3811
Author(s):  
Youxia Wang ◽  
Xiaoling Chen ◽  
Zhiqing Huang ◽  
Daiwen Chen ◽  
Bing Yu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Ferulic Acid ◽  
Antioxidant Capacity ◽  
Protein Level ◽  
Longissimus Dorsi ◽  
Mrna Levels ◽  
Weaned Piglets ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Protein Expressions

Ferulic acid (FA) is a phenolic compound that has antioxidant, hepatoprotective, anticarcinogenic, anti-inflammatory, antiallergic, antimicrobial, antiviral, and vasodilatory effects. This study was conducted to explore the effects of dietary FA supplementation on antioxidant capacity and lipid metabolism in weaned piglets. Eighteen 21-day-old castrated male DLY (Duroc × Landrace × Yorkshire) weaned piglets were randomly divided into control, 0.05%, and 0.45% FA groups. The results showed that, in serum, CAT and T-SOD activities and content of HDL-C were increased, but the content of MDA and the activities of T-CHO and LDL-C were decreased, by FA supplementation. In liver, dietary FA supplementation increased CAT, T-SOD, and GSH-PX activities and upregulated the mRNA levels of SOD1, SOD2, CAT, GST, GPX1, GR, Nrf2, HSL, CPT1b, and PPARα but decreased the contents of MDA and TG. Furthermore, dietary FA supplementation increased the protein level of Nrf2, HO-1, and NQO-1. In longissimus dorsi muscle, dietary FA supplementation increased the activity of T-SOD and the mRNA abundance of SOD1, SOD2, CAT, GST, GPX1, GR, and Nrf2 but decreased the contents of MDA and T-CHO. Additionally, dietary FA supplementation increased the protein expressions of Nrf2, HO-1, and NQO1. Together, our data suggest that FA could improve antioxidant capacity and lipid metabolism in weaned piglets.

scholarly journals Influence of Hormones on mRNA Abundance of Enzymes Related Lipid Metabolism in Longissimus Dorsi Muscle in vitro

2012 ◽  
Vol 11 (3) ◽  
pp. 386-392
Author(s):  
Lian Hong-Xia ◽  
Lu De-Xun ◽  
Gao Min ◽  
Gao Teng-Yun
Keyword(s):  
Lipid Metabolism ◽  
Mrna Abundance ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle

scholarly journals The response of gene expression associated with lipid metabolism, fat deposition and fatty acid profile in the longissimus dorsi muscle of Gannan yaks to different energy levels of diets

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187604 ◽  
Author(s):  
Chao Yang ◽  
Jianbin Liu ◽  
Xiaoyun Wu ◽  
Pengjia Bao ◽  
Ruijun Long ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fatty Acid Profile ◽  
Energy Levels ◽  
Fat Deposition ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle

In vivo evidence on the functional variation within fatty acid synthase gene associated with lipid metabolism in bovine longissimus dorsi muscle tissue

2017 ◽  
Vol 40 (3) ◽  
pp. 289-294
Author(s):  
Dong-yep Oh ◽  
Insik Nam ◽  
Sehwan Hwang ◽  
Hongsik Kong ◽  
Honggu Lee ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Muscle Tissue ◽  
Fatty Acid Synthase ◽  
Longissimus Dorsi ◽  
Functional Variation ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Fatty Acid Synthase Gene

A Technique for Measuring the Cross-Sectional Axes of the Longissimus Dorsi Muscle and Fat Depth in Lambs1,2

1960 ◽  
Vol 19 (3) ◽  
pp. 803-809
Author(s):  
D. J. Matthews ◽  
R. A. Merkel ◽  
J. D. Wheat ◽  
R. F. Cox
Keyword(s):  
Longissimus Dorsi ◽  
Cross Sectional ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
The Cross

Intrauterine Growth Restriction Alters the Genome-Wide DNA Methylation Profiles in Small Intestine, Liver and Longissimus Dorsi Muscle of Newborn Piglets

2019 ◽  
Vol 20 (7) ◽  
pp. 713-726 ◽  
Author(s):  
Shiyu Tao ◽  
Tianjiao Zhou ◽  
Perot Saelao ◽  
Ying Wang ◽  
Yuhua Zhu ◽  
...  
Keyword(s):  
Small Intestine ◽  
Birth Weight ◽  
Intrauterine Growth Restriction ◽  
Longissimus Dorsi ◽  
Intrauterine Growth ◽  
Longissimus Dorsi Muscle ◽  
Newborn Piglets ◽  
Dorsi Muscle ◽  
Genome Wide ◽  
Differentially Methylated Genes

Intrauterine growth restriction (IUGR) remains a major problem in swine production since the associated low birth weight leads to high rates of pre-weaning morbidity and mortality, and permanent retardation of growth and development. The underlying regulatory mechanisms from the aspects of epigenetic modification has received widespread attention. Studies explore the changes in genome wide methylation in small intestine (SI), liver and longissimus dorsi muscle (LDM) between IUGR and normal birth weight (NBW) newborn piglets using a methylated DNA immunoprecipitation-sequencing (MeDIP-Seq) approach. The data demonstrated that methylated peaks were prominently distributed in distal intergenic regions and the quantities of peaks in IUGR piglets were more than that of NBW piglets. IUGR piglets had relatively high methylated level in promoters, introns and coding exons in all the three tissues. Through KEGG pathway analysis of differentially methylated genes found that 33, 54 and 5 differentially methylated genes in small intestine, liver and longissimus dorsi muscle between NBW and IUGR piglets, respectively, which are related to development and differentiation, carbohydrate and energy metabolism, lipid metabolism, protein turnover, immune response, detoxification, oxidative stress and apoptosis pathway. The objective of this review is to assess the impact of differentially methylation status on developmental delay, metabolic disorders and immune deficiency of IUGR piglets.

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