scholarly journals A Missense Mutation of the HSPB7 Gene Associated with Heat Tolerance in Chinese Indicine Cattle

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 554 ◽  
Author(s):  
Lulan Zeng ◽  
Yanhong Cao ◽  
Zhuyue Wu ◽  
Mingguang Huang ◽  
Guoliang Zhang ◽  
...  
Keyword(s):  
Heat Shock ◽  
Missense Mutation ◽  
Heat Tolerance ◽  
Mean Annual Temperature ◽  
Indigenous Cattle ◽  
Cattle Breeds ◽  
Candidate Mutation ◽  
Humidity Index ◽  
Polymerase Chain ◽  
Shock Proteins

The small heat shock proteins (HSPB) are expressed in response to heat stress, and the heat shock protein family B (small) member 7 (HSPB7) gene has been reported to play an important role in heat tolerance pathways. Only a missense mutation (NC_037329.1: g.136054902 C > G: p.Ala69Gly) was identified in the HSPB7 gene in indicine cattle, which might be a candidate mutation associated with the heat tolerance. Here, we explore the allele frequency of this mutation in 774 individuals belonging to 32 Chinese indigenous cattle breeds using polymerase chain reaction (PCR) and DNA sequencing methods. The distribution of alleles of NC_037329.1: g.136054902 C > G displays significant geographical difference across native Chinese cattle breeds that the allele C was dominant in northern cattle groups, while allele G was dominant in southern indicine cattle groups. Additionally, the association analysis indicated that the G allele was significantly associated with mean annual temperature (T), relative humidity (RH), and temperature humidity index (THI) (p < 0.01), suggesting that cattle carrying allele G were distributed in regions with higher T, RH, and THI. Our results demonstrate that the mutation of the HSPB7 gene in Chinese indicine cattle might be a candidate gene associated with the heat tolerance.

scholarly journals MTOR Variation Related to Heat Resistance of Chinese Cattle

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 915 ◽  
Author(s):  
Qingqing Ning ◽  
Kaixing Qu ◽  
Quratulain Hanif ◽  
Yutang Jia ◽  
Haijian Cheng ◽  
...  
Keyword(s):  
Heat Tolerance ◽  
Mean Annual Temperature ◽  
Opposite Pattern ◽  
Indigenous Cattle ◽  
Cattle Breeds ◽  
Chinese Cattle ◽  
Damage Repair ◽  
Temperature Heat ◽  
Humidity Index ◽  
Hot Environments

With the inexorable rise of global temperature, heat stress deserves more and more attention in livestock agriculture. Previous studies have shown that the mechanistic target of rapamycin (MTOR) (NC_037343.1:c.2062G>C) gene contributes to the repair of DNA damage repair and is associated with the adaptation of camels in dry and hot environments. However, it is unknown whether this mutation is related to the heat tolerance of Chinese cattle. In this study, PCR and sequencing were used to type the mutation locus in 1030 individuals of 37 cattle breeds. The analysis results showed that the frequency of G allele of the locus gradually diminished from the northern group to the southern group of native Chinese cattle, whereas the frequency of the C allele showed an opposite pattern, displaying a significant geographical difference across native Chinese cattle breeds. Additionally, an analysis of the locus in Chinese indigenous cattle revealed that this SNP was significantly associated with mean annual temperature (T), relative humidity (RH) and temperature humidity index (THI) (p < 0.01), suggesting that cattle with C allele was distributed in regions with higher T, RH and THI. In conclusion, this study proved that the mutation of MTOR gene in Chinese cattle could be associated with the heat tolerance.

scholarly journals Transcriptional regulation of heat shock proteins and ascorbate peroxidase by CtHsfA2b from African bermudagrass conferring heat tolerance in Arabidopsis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiuyun Wang ◽  
Wanlu Huang ◽  
Zhimin Yang ◽  
Jun Liu ◽  
Bingru Huang
Keyword(s):  
Transcriptional Regulation ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Ascorbate Peroxidase ◽  
Heat Tolerance ◽  
Shock Proteins

Construction of an internal standard for semi-quantitative polymerase chain reaction analysis of heat shock proteins

1996 ◽  
Vol 17 (1) ◽  
pp. 40-43 ◽  
Author(s):  
Mahboob A. Khan ◽  
Najma Latif ◽  
Paola Petrobelli ◽  
Magdi H. Yacoub ◽  
Michael J. Dunn
Keyword(s):  
Polymerase Chain Reaction ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Quantitative Polymerase Chain Reaction ◽  
Internal Standard ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Shock Proteins

The Heat-Shock Response and Expression of Heat-Shock Proteins in Wheat Under Diurnal Heat Stress and Field Conditions

1994 ◽  
Vol 21 (6) ◽  
pp. 857 ◽  
Author(s):  
HT Nguyen ◽  
CP Joshi ◽  
N Klueva ◽  
J Weng ◽  
KL Hendershot ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Tolerance ◽  
Heat Shock Response ◽  
Field Conditions ◽  
Shock Response ◽  
Heat Tolerant ◽  
Shock Proteins

The occurrence of heat-shock proteins (HSPs) in response to high temperature stress is a universal phenomenon in higher plants and has been well documented. However, in agriculturally important species, less is known about the expression of HSPs under natural environments. A review of the heat-shock response in wheat (Triticum aestivum L.) is presented and recent results on the expression of wheat HSPs under diurnal stress and field conditions are reported. In the field experiment, flag leaf blade temperatures were obtained and leaf blades collected for northern blot analysis using HSP 16.9 cDNA as a probe. Temperatures of leaf blades ranged from 32 to 35�C under the tested field conditions at New Deal near Lubbock, Texas. Messenger RNAs encoding a major class of low molecular weight HSPs, HSP 16.9, were detected in all wheat genotypes examined. The results suggested that HSPs are synthesised in response to heat stress under agricultural production, and furthermore, that HSPs are produced in wheats differing in geographic background. In the controlled growth chamber experiment, HSP expression in two wheat cultivars, Mustang (heat tolerant) and Sturdy (heat susceptible) were analysed to determine if wheat genotypes differing in heat tolerance differ in in vitro HSP synthesis (translatable HSP mRNAs) under a chronic, diurnal heat-stress regime. Leaf tissues were collected from seedlings over a time-course and poly (A)+RNAs were isolated for in vitro translation and 2-D gel electrophoresis. The protein profiles shown in the 2-D gel analysis revealed that there were not only quantitative differences of individual HSPs between these two wheat lines, but also some unique HSPs which were only found in the heat tolerant line. This data provides evidence of a correlation between HSP synthesis and heat tolerance in wheat under a simulated field environment and suggests that further genetic analysis of HSPs in a segregating population is worthy of investigation. In conclusion, the results of this study provide an impetus for the investigation of the roles of HSP genes in heat tolerance in wheat.

scholarly journals Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves inAgave tequilanavar. Weber

2009 ◽  
Vol 32 (12) ◽  
pp. 1791-1803 ◽  
Author(s):  
ROSARIO LUJÁN ◽  
FERNANDO LLEDÍAS ◽  
LUZ MARÍA MARTÍNEZ ◽  
RITA BARRETO ◽  
GLADYS I. CASSAB ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Tolerance ◽  
Cooling Capacity ◽  
Small Heat Shock Proteins ◽  
Shock Proteins

Melatonin improves heat tolerance in Actinidia deliciosa via carotenoid biosynthesis and heat shock proteins expression

2021 ◽  
Author(s):  
Hui Xia ◽  
Yuanjie Zhou ◽  
Honghong Deng ◽  
Lijin Lin ◽  
Qunxian Deng ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Tolerance ◽  
Carotenoid Biosynthesis ◽  
Actinidia Deliciosa ◽  
Shock Proteins

scholarly journals INFLUENCE OF HIGH TEMPERATURES ON HEAT TOLERANCE AND SYNTHESIS OF HEAT SHOCK PROTEINS IN SPRING WHEAT AT THE INITIAL STAGES OF DEVELOPMENT

2020 ◽  
Vol 12 (5) ◽  
pp. 179
Author(s):  
Olga Andreevna Fedotova ◽  
Elizaveta Alekseevna Polyakova ◽  
Olga Ivanovna Grabelnykh
Keyword(s):  
Triticum Aestivum ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
High Temperatures ◽  
Heat Tolerance ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Stages Of Development ◽  
Shock Proteins

Пшеница одна из сельскохозяйственных культур, которая подвергается действию температурного стресса в течение онтогенеза и особенно чувствительна к действию стресса на начальных стадиях её развития. Высокие температуры ограничивают рост, развитие и продуктивность растений. Однако, растения могут адаптироваться к температурному стрессу и имеют адаптивные механизмы на клеточном и молекулярном уровнях. Одним из таких адаптивных механизмов является увеличение синтеза белков теплового шока (БТШ), функции которых очень важны и разнообразны.Целью данной работы было изучить влияние различных тепловых обработок (37 и 39 ºC в течение 1, 3, 6, 24 ч и 50 ºC в течение 1, 3, 5 ч) на теплоустойчивость проростков яровой пшеницы (Triticum aestivum L.) и синтез БТШ (101, 70, 60 и 16.9 кДа) в побегах 4-х суточных и листьях 8-ми суточных проростков.На основании полученных данных сделан вывод, что 4-х суточные и 8-ми суточные проростки яровой пшеницы характеризуются схожей реакцией на тепловой стресс при 50 ºC, однако 4-х суточные проростки более устойчивы в начальный период стрессового воздействия. Воздействие температурами 37 и 39 ºC подходят для адаптации яровой пшеницы на начальных стадиях её развития (4-х суточные и 8-ми суточные проростки). Эти температуры приводят к схожей тенденции в синтезе БТШ в побегах и листьях. В тоже время, содержание БТШ16.9 зависит от стадии развития пшеницы. В нормальных условиях был отмечен синтез БТШ16.9 в побегах, но не в листьях. Сделано заключение, что адаптация 8-ми суточных проростков более длительный процесс (6-24 ч воздействия 37 и 39 ºC) по сравнению с 4-х суточными проростками (3-6 ч воздействия 37 и 39 ºC).

scholarly journals Evaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle Breeds

2020 ◽  
Vol 15 (1) ◽  
pp. 32-42
Author(s):  
Gbolabo Onasanya ◽  
George Msalya ◽  
Aranganoor Thiruvenkadan ◽  
Chirukandoth Sreekumar ◽  
Krishnaswamy Tirumurugaan ◽  
...  
Keyword(s):  
Heat Shock ◽  
High Resolution ◽  
Heat Shock Protein ◽  
Heat Tolerance ◽  
High Resolution Melting ◽  
Heat Shock Protein 90 ◽  
Zebu Cattle ◽  
Cattle Breeds
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