scholarly journals Pilot Study on Alteration of LA-MRSA Status of Pigs during Fattening Period on Straw Bedding by Two Types of Cleaning

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 521
Author(s):  
Hannah Schollenbruch ◽  
Iris Kobusch ◽  
Iris Schröter ◽  
Alexander Mellmann ◽  
Robin Köck ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pilot Study ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Air Samples ◽  
Time Points ◽  
Fattening Period ◽  
Cleaning And Disinfection ◽  
Pig Husbandry ◽  
Fattening Pigs

In countries with professional pig husbandry in stables, the prevalence of livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) on farms has remained high or has further increased in recent years. Simple measures to reduce LA-MRSA among pigs have not yet been successfully implemented. The aim of this pilot study is twofold: first, to examine how the LA-MRSA status of LA-MRSA positive fattening pigs at the date of housing changes over the fatting period on straw bedding and, second, whether this change could be influenced by the quality of cleaning and disinfection (C&D). For this purpose, 122 animals are individually tested for LA-MRSA carriage at five sequential time points comparing pigs housed on a farm using straw bedding plus C&D (n = 59) vs. straw bedding plus simple cleaning (n = 63). At the time of housing, all animals in both groups are LA-MRSA positive. This status changes to 0% in the group with simple cleaning until the end of fattening and 28% in the C&D group. LA-MRSA in environmental and air samples is also reduced over the fattening period. The results indicate that keeping pigs on straw might be one way to reduce LA-MRSA during the fattening period with simple cleaning appearing to be more beneficial than C&D. Further investigations are necessary to determine the influencing factors more precisely.

scholarly journals Single Blinded Study on the Feasibility of Decontaminating LA-MRSA in Pig Compartments under Routine Conditions

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 141
Author(s):  
Iris Kobusch ◽  
Hannah Müller ◽  
Alexander Mellmann ◽  
Robin Köck ◽  
Marc Boelhauve
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Great Effort ◽  
Methicillin Resistant ◽  
Air Samples ◽  
Blinded Study ◽  
Before And After ◽  
Cleaning And Disinfection ◽  
Routine Cleaning ◽  
Pig Husbandry

In countries with intensive pig husbandry in stables, the prevalence of livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) on such farms has remained high in the last few years or has also further increased. Simple measures to reduce the LA-MRSA among pigs have not yet been successfully implemented. Earlier publications showed a decontamination of LA-MRSA was only possible with great effort. The aim of this study is to determine the suitability of routine cleaning and disinfection (C&D) for adequate LA-MRSA decontamination. For this purpose, at least 115 locations in a piglet-rearing compartment were examined before and after cleaning and disinfection. The sample locations were stratified according to accessibility for pigs and the difficulty of cleaning. The cleaning work was carried out routinely by farm employees, who were not informed about the sampling (single blinded). While before cleaning and disinfection, 85% of the samples from the surfaces were LA-MRSA positive, while only 2% were positive thereafter. All LA-MRSA-positive samples after cleaning and disinfection were outside the animal area. Air samples also showed no LA-MRSA after cleaning and disinfection. Conclusion: In well-managed livestock farms, decontamination of the LA-MRSA barn is quite possible; after C&D no LA-MRSA was detectable at animal height.

Fourier-Transform Infrared (FTIR) Spectrophotometry: An Ecofriendly Method for the Analysis of Injectable Daptomycin

2017 ◽  
Vol 100 (5) ◽  
pp. 1569-1576 ◽  
Author(s):  
Eliane Gandolpho Tótoli ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sustainable Consumption ◽  
Toxic Waste ◽  
Vancomycin Resistant Enterococci ◽  
Ir Spectrophotometry ◽  
Vancomycin Resistant ◽  
Ftir Spectrophotometry ◽  
The Impact

Abstract Daptomycin (DPT) is an important antimicrobial agent used in clinical practice because it is very active against several types of medicinally challengingGram-positive bacteria, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci. In addition to concerns about the quality of the analytical methods used in the QC of drugs, there is also concern about the impact of these methods on the environment. The trend toward sustainable consumption is increasingly evident and has forced the pharmaceutical industry to reduce the generation of toxic waste. Inthis context, IR spectrophotometry stands out because it does not use organic solvents and, although it is formally accepted for the identification of individual compounds, also allows the quantification of substances. Therefore, the aim of this work was to develop and validate a green analytical method for theanalysis of DPT in a lyophilized powder for injection by FTIR spectrophotometry. The method involved absorbance measurements in the spectral region of 1700–1600 cm−1. The method was properly validated and found to be linear, precise, accurate, selective, and robust for the concentrationrange between 0.2 and 0.6 mg/150 mg. The validated method was able to quantify DPT powder for injection and can be used as an environmentally friendly alternative for routine analysis in QC.

scholarly journals 712. Activity of Exebacase (CF-301) Against Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilms on Orthopedic Kirschner Wires

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S320-S320
Author(s):  
Melissa J Karau ◽  
Suzannah Schmidt-Malan ◽  
Jayawant Mandrekar ◽  
Dario Lehoux ◽  
Raymond Schuch ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bactericidal Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Kirschner Wires ◽  
Carrier Solution ◽  
Time Points ◽  
Significant Difference ◽  
Peptidoglycan Cell Wall ◽  
The Media ◽  
K Wires

Abstract Background Orthopedic foreign body-associated infection can be difficult to treat due to the formation of biofilms protecting microorganisms from both antimicrobials and the immune system. Exebacase (EXE) is a phage-derived lysin which acts as a direct lytic agent by hydrolyzing the peptidoglycan cell wall of Staphylococcus aureus. In this study, the activity of EXE was evaluated in comparison to daptomycin against MRSA biofilms on orthopedic Kirschner wires (K-wires). Methods MRSA strain IDRL-6169 was studied; it has a MIC of 0.5 µg/mL for both daptomycin (DAP) and EXE. Biofilms were formed in 1 mL of 106 cfu/mL tryptic soy broth on 0.5x0.1 mm threaded stainless steel K-wires for 10 hours, after which the wires were removed from the media and placed into 0.04 mL of either DAP or EXE at 0 (vehicle only), 0.098, 0.98, or 9.8 mg/mL. DAP+EXE was also tested, each at 0.098 mg/mL. Bacteria were quantified after 0, 2, 4, 8, and 12 hours of incubation at 37ºC. Testing was performed in triplicate. Results were reported as log10 cfu/K-wire reduction relative to vehicle alone. A 3-log10 cfu/K-wire reduction was considered bactericidal. P-values were calculated using Kruskal–Wallis. Results The bacterial burden of vehicle alone ranged from 5.49- to 6.33-log10 cfu/K-wire at all time points. Bacterial reductions for each treatment compared with carrier solution are shown in the table. DAP showed no bactericidal activity. EXE showed bactericidal activity at all concentrations at all time points studied except 0.098 mg/mL at 8 hours. There was no significant difference between EXE at 0.098 and 0.98 mg/mL at any time point but EXE at 9.8 mg/mL did show superiority over the lower concentrations. DAP+EXE 0.098 mg/mL was bactericidal at all time points. Conclusion EXE showed a rapid effect against MRSA biofilms on orthopedic K-wires apparent within the first 2 hours of exposure and was more active than daptomycin alone at the same concentrations. Disclosures All authors: No reported disclosures.

scholarly journals Dynamic of Livestock-Associated Methicillin-Resistant Staphylococcus aureus CC398 in Pig Farm Households: A Pilot Study

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e65512 ◽  
Author(s):  
Cristina Garcia-Graells ◽  
Brigitte A. G. L. van Cleef ◽  
Jesper Larsen ◽  
Olivier Denis ◽  
Robert Skov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pilot Study ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Farm Households ◽  
Methicillin Resistant ◽  
Pig Farm

Methicillin-Resistant Staphylococcus aureus CC398 in Purulent Lesions of Piglets and Fattening Pigs in Portugal

2020 ◽  
Vol 26 (7) ◽  
pp. 850-856 ◽  
Author(s):  
Vânia Santos ◽  
Anícia Gomes ◽  
Laura Ruiz-Ripa ◽  
Olouwafemi Mistourath Mama ◽  
Carolina Sabença ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Fattening Pigs

Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis

2021 ◽  
Vol 14 (2) ◽  
pp. 14-20
Author(s):  
Abdullah Abdullazeez Sheehan ◽  
Mohammed Khudor ◽  
Fanar Isihak
Keyword(s):  
Staphylococcus Aureus ◽  
Pilot Study ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lethal Dose ◽  
Challenge Test ◽  
Albino Rats ◽  
Protection Level ◽  
Methicillin Resistant ◽  
Starting Dose ◽  
Wistar Albino Rats

It is very important, before starting the manufacture of any vaccine from any microorganism estimation of LD₅₀ of that microorganism to determine their pathogenicity and virulence. Estimated LD₅₀ was very important to be used in challenge tests later to estimate the protection level of the manufactured vaccine in experimental animals. So, this study was aimed to estimate LD₅₀ of local methicillin-resistant Staphylococcus aureus (MRSA) bacterial isolate. A pilot study has been done to determine approximately LD₅₀ of used MRSA in the study by using different bacterial concentrations of MRSA to determine approximate LD₅₀ that can be able to kill half numbers of animals used in the study to be used later in the estimation of exact LD₅₀ by using of Up-and-Down method. Ninety Wistar albino rats have been used for this purpose, eighty-four animals which divided into fourteen groups by six animals for each group (for pilot study) and remained six animals for (Up-and-Down method). The results showed that 9 X 10¹⁰ CFU/ml was led to killing half number of animals used in the study, this dose has been used as starting dose in the Up-and-Down method to the estimation of the exact LD₅₀ dose. The results showed that 5.526 X 10¹⁰ CFU/ml was the exact LD₅₀ of local MRSA isolate, which will be used later in the challenge test to estimate the protection level of a locally prepared vaccine against MRSA isolate.

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