scholarly journals Study of Disinfectant Resistance Genes in Ocular Isolates of Pseudomonas aeruginosa

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 88 ◽  
Author(s):  
Dinesh Subedi ◽  
Ajay Vijay ◽  
Mark Willcox
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Resistance Genes ◽  
Contact Lens ◽  
Contact Lenses ◽  
Microbial Keratitis ◽  
Whole Genomes ◽  
Microdilution Broth ◽  
Disinfectant Resistance

Background: The prevalence of disinfectant resistance in Pseudomonas aeruginosa is on the rise. P. aeruginosa is the most common bacteria isolated from cases of microbial keratitis. Many multi-purpose contact lens disinfectant solutions are available to decontaminate contact lenses before use and to help reduce the incidence of infections. However, with increasing disinfectant resistance, the effect of multi-purpose disinfectant solutions may diminish. The goal of this study was to examine genes associated with disinfectant resistance in ocular isolates of P. aeruginosa and understand the strain’s susceptibility to different multipurpose disinfectant solutions. Methods: Seven potential disinfectant resistance genes were used in BLASTn searches against the whole genomes of 13 eye isolates of P. aeruginosa. A microdilution broth method was used to examine susceptibility to four different multipurpose disinfectant solutions. Results: All strains possessed the sugE2, sugE3 and emrE (qacE) genes. The sugE1 and qacEdelta1 genes were present in 6/13 isolates. No strains contained the qacF or qacG genes. All tested disinfectant solutions had the ability to kill all test strains at 100% concentration, with some strains being susceptible at 1:8 dilutions of the disinfecting solutions. However, the presence of disinfectant resistance genes was not associated with susceptibility to multi-purpose disinfectants. Conclusion: All four tested contact lens disinfectant preparations are effective against P. aeruginosa isolates regardless of the presence of disinfectant resistance genes.

scholarly journals Contact Lens Associated Bacterial Keratitis: Common Organisms, Antibiotic Therapy, and Global Resistance Trends: A Systematic Review

2021 ◽  
Vol 1 ◽  
Author(s):  
Hossein Hatami ◽  
Amir Ghaffari Jolfayi ◽  
Ali Ebrahimi ◽  
Saeid Golmohammadi ◽  
Moein Zangiabadian ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Therapy ◽  
Contact Lens ◽  
Contact Lenses ◽  
Bacterial Keratitis ◽  
Microbial Keratitis ◽  
Surgical Interventions ◽  
Geographical Regions ◽  
Almost All

IntroductionContact lens wearing has been increased globally during recent decades, which is one of the main risk factors for developing microbial keratitis. Microbial keratitis is a severe and dangerous condition that causes cornea inflammation. It can lead to corneal scarring and perforation or even endophthalmitis and visual loss if it remains untreated. Among bacterial, fungal, protozoal, and viral agents which can cause microbial keratitis, bacteria are the most common cause. Therefore, in this study, we aim to find common causative bacteria, sensitivity, and resistance to antibiotics and the outcome of antibiotic therapy in contact lens-related bacterial keratitis.MethodsA systematic search was carried out in PubMed/Medline, EMBASE, and Web of Science for published studies and medRxiv for preprints up to February 30, 2021, and May 14, 2021, respectively. A combination of the following keywords was used: “Infection”, “Corneal infection”, “Keratitis”, “Microbial keratitis”, and “Contact lens”, Also, we used the “Contact lenses” MeSH term. Lists of references for each selected article and relevant review articles were hand-searched to identify further studies.ResultsTwenty-six articles were included. From 1991 to 2018, 2,916 episodes of contact lens-related microbial keratitis) CLMK(with 1,642 episodes of proven bacterial keratitis have been reviewed in these studies. Studies were conducted in 17 countries with different geographical regions, and four studies were conducted in Iran, which is the highest number of studies among these countries. According to 20 studies, the mean age of patients was 30.77 years. Females with 61.87% were more than males in 19 studies. A percentage of 92.3% of patients used soft contact lenses, and 7.7% of patients used hard contact lenses (including RGP), according to 16 studies. Pseudomonas aeruginosa, Staphylococcus spp., and Serratia marcescens were the three most common bacteria isolated from samples of patients with contact lens-related bacterial keratitis. Overall, isolated bacteria were most sensitive to fluoroquinolones and aminoglycosides, especially ciprofloxacin and gentamicin respectively, and most resistant against penicillin and cephalosporins especially cefazolin and chloramphenicol. Almost all patients responded well to antibiotic therapy, with some exceptions that needed further surgical interventions.ConclusionAntibiotics are efficient for treating almost all patients with contact lens-related bacterial keratitis if they are appropriately chosen based on common germs in every geographical region and the sensitivity and resistance of these germs against them. In this regard, Pseudomonas aeruginosa is the most common causative germ of contact lens-associated bacterial keratitis all over the world and is almost fully sensitive to ciprofloxacin. Because of some different results about the sensitivity and resistance of germs against some antibiotics like gentamicin, vancomycin, and chloramphenicol in the Middle East region, especially Iran, more in vitro and clinical studies are suggested.

scholarly journals Personal hygiene risk factors for contact lens-related microbial keratitis

2020 ◽  
Vol 5 (1) ◽  
pp. e000476
Author(s):  
Anna Stellwagen ◽  
Cheryl MacGregor ◽  
Roger Kung ◽  
Aristides Konstantopoulos ◽  
Parwez Hossain
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Contact Lens ◽  
Contact Lenses ◽  
Healthcare Services ◽  
University Hospital ◽  
Personal Hygiene ◽  
Microbial Keratitis ◽  
Contact Lens Wear ◽  
Lens Wear

ObjectiveMicrobial keratitis is a sight-threatening complication of contact lens wear, which affects thousands of patients and causes a significant burden on healthcare services. This study aims to identify compliance with contact lens care recommendations and identify personal hygiene risk factors in patients who develop contact lens-related microbial keratitis.Methods and analysisA case–control study was conducted at the University Hospital Southampton Eye Casualty from October to December 2015. Two participant groups were recruited: cases were contact lens wearers presenting with microbial keratitis and controls were contact lens wearers without infection. Participants underwent face-to-face interviews to identify lens wear practices, including lens type, hours of wear, personal hygiene and sleeping and showering in lenses. Univariate and multivariate regression models were used to compare groups.Results37 cases and 41 controls were identified. Showering in contact lenses was identified as the greatest risk factor (OR, 3.1; 95% CI, 1.2 to 8.5; p=0.03), with showering daily in lenses compared with never, increasing the risk of microbial keratitis by over seven times (OR, 7.1; 95% CI, 2.1 to 24.6; p=0.002). Other risks included sleeping in lenses (OR, 3.1; 95% CI, 1.1 to 8.6; p=0.026), and being aged 25–39 (OR, 6.38; 95% CI, 1.56 to 26.10; p=0.010) and 40–54 (OR, 4.00; 95% CI 0.96 to 16.61; p=0.056).ConclusionThe greatest personal hygiene risk factor for contact lens-related microbial keratitis was showering while wearing lenses, with an OR of 3.1, which increased to 7.1 if patients showered daily in lenses. The OR for sleeping in lenses was 3.1, and the most at-risk age group was 25–54.

scholarly journals Severe Keratitis Caused byPseudomonas aeruginosaSuccessfully Treated with Ceftazidime Associated with Acetazolamide

2009 ◽  
Vol 2009 ◽  
pp. 1-3 ◽  
Author(s):  
Benoit Hue ◽  
Marc Doat ◽  
Gilles Renard ◽  
Marie-Laure Brandely ◽  
François Chast
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Case Report ◽  
Carbonic Anhydrase ◽  
Oral Administration ◽  
Anterior Chamber ◽  
Aqueous Humor ◽  
Contact Lens ◽  
Eye Drops ◽  
Antibiotic Regimen ◽  
Microbial Keratitis

Purpose. To report a case of microbial keratitis caused by Pseudomonas aeruginosa treated with a combination of acetazolamide and ceftazidime.Methods. Case report.Results. We report the case of a 17-year-old contact lens-wearing female who developed severe keratitis due toPseudomonas aeruginosatemporarily healed with topical fortified antibiotic eye drops. After few days, the patient relapsed, and topical and intravenous ceftazidime were added. Concomitantly, oral administration of acetazolamide was prescribed. This carbonic anhydrase inhibitor was added to the antibiotic regimen in order to decrease the anterior chamber pH, and then, the ceftazidime ionization. By lowering the state of ionization of the antibiotic in the aqueous humor, its concentration was increased. This was confirmed by an improvement of the patient within few days and a rapid eradication of the infection.Conclusion. This is the first reported case of keratitis caused byP. aeruginosasuccessfully treated using acetazolamide as an enhancer of ceftazidime effectiveness.

scholarly journals Comparison of the Antimicrobial Efficacy of Various Contact Lens Solutions to Inhibit the Growth of Pseudomonas aeruginosa and Staphylococcus aureus

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
B. Laxmi Narayana ◽  
Pooja Rao ◽  
Sevitha Bhat ◽  
K. Vidyalakshmi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Contact Lens ◽  
Contact Lenses ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Test Method ◽  
Medical College ◽  
Soft Contact Lenses ◽  
Contact Lens Solution

Soft contact lenses provide perfect conditions for the breeding of pathogens. The study is a prospective, experimental study, conducted to know the antimicrobial ability of multipurpose contact lens solution against standard strains of Staphylococcus aureus and Pseudomonas aeruginosa by the stand-alone test. The test method is based on the procedures in the ISO 14729 standard primary stand-alone test. Three multipurpose contact lens care solutions commercially available in Mangalore markets, namely, Biotrue (Bausch & Lomb), Opti-Free Replenish (Alcon), and Aquasoft (Stericon Pharma), were tested for its antimicrobial effect in the microbiology lab at Kasturba Medical College, Mangalore. According to this study, the solutions named “Biotrue” and “Aquasoft” met the primary stand-alone and reached the 3log reduction and 5log reduction criteria in the manufacturer recommended time, respectively. No conclusion could be drawn for Opti-Free Replenish since the minimum recommended disinfection time was overnight, whereas it was noted for 6 hr only, and it should have been experimented further. The effectiveness of multipurpose solutions varies against different bacterial species. We have observed that the antimicrobial activity of different solutions varies with respect to time of incubation, and also there was a marked difference in the activity of some solutions against S. aureus and P. aeruginosa. So, it is necessary for the contact lens users to store their lenses in solutions for longer duration of hours. It is also recommended to use solutions that clear the ISO 14729 standards for better health conditions of the eye.

scholarly journals Microbial Keratitis Profile at a University Hospital in Hong Kong

2014 ◽  
Vol 2014 ◽  
pp. 1-4
Author(s):  
Tracy H. T. Lai ◽  
Vishal Jhanji ◽  
Alvin L. Young
Keyword(s):  
Risk Factors ◽  
Pseudomonas Aeruginosa ◽  
Risk Factor ◽  
Hong Kong ◽  
Contact Lens ◽  
University Hospital ◽  
Major Risk Factor ◽  
Sampled Data ◽  
Microbial Keratitis ◽  
Contact Lens Wear

Purpose. To evaluate the recent trends in demographics, risk factors, and microbiological profiles of microbial keratitis at a university hospital in Hong Kong. Design. Retrospective review. Methods. The medical records of 51 patients admitted to the Prince of Wales Hospital for microbial keratitis from January 2010 to June 2012 were reviewed. Demographics, risk factors, clinical features, microbiological results, and treatment were recorded. Data was analyzed and compared to our historical sampled data collected 11 years ago. Results. The mean age of patients was 41.6 ± 20.3 years. Contact lens use was the major risk factor (45%), followed by injury (12%). The culture positive rate was 59%, of which 37% were Gram-positive organisms and 53% were Gram-negative organisms. Pseudomonas aeruginosa (50%) and coagulase-negative Staphylococcus (13%) were the most commonly isolated pathogens. No resistance to fluoroquinolones was identified. Conclusions. Our study showed that contact lens wear remained the major risk factor for microbial keratitis in Hong Kong and Pseudomonas aeruginosa remained the commonest bacterium isolated. This is comparable to our historical data and other studies conducted in East Asia.

Effect of Pseudomonas aeruginosa Concentration in Experimental Contact Lens--Related Microbial Keratitis

Cornea ◽  
1993 ◽  
Vol 12 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Christiane A. Lawin-Brüssel ◽  
Miguel F. Refojo ◽  
Fee-Lai Leong ◽  
Laila Hanninen ◽  
Kenneth R. Kenyon
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contact Lens ◽  
Microbial Keratitis

scholarly journals Bacterial adhesion to Conventional and Silicone hydrogel contact lenses

2021 ◽  
Vol 23 (1) ◽  
pp. 25-35
Author(s):  
Eghosasere Iyamu ◽  
Frederick Osaro Ekhaise
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Contact Lens ◽  
Bacterial Adhesion ◽  
Contact Lenses ◽  
Bacterial Species ◽  
Soft Contact ◽  
Silicone Hydrogel ◽  
Soft Contact Lenses ◽  
Lens Wear

Purpose: The aim of this study was to investigate the adhesion of bacteria to worn silicone hydrogel and conventional soft contact lenses.Methods: Bacterial adhesion experiments / assays were performed on 24 worn and 6 unworn soft contact lenses each of different materials (high- and low- gas permeable lenses) using the strains such as Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923.Results: P. aeruginosa adhered in increased number to worn than unworn Lotrafilcon A and conventional lenses. However, a higher number of P. aeruginosa adhered to unworn than worn Lotrafilcon B, the difference in the mean adhesion was not significant (p = 0.66). S. aureus adhered in significantly decreased number to worn Lotrafilcon A, nelfilcon A, nesofilcon A, etafilcon A and omafilcon A (p<0.05); butsignificantly higher number adhered to worn than unworn polymacon (p<0.05). Lens wear had no effect on the adhesion of S. aureus to Lotrafilcon B (p>0.05). The least adhesion of P. aeruginosa to worn contact lenses was seen with polymacon, while S. aureus adhered in least number to worn Lotrafilcon A compared to the other contact lens materials that demonstrated the same trend in adhesion.Conclusion: The higher adhesion of P. aeruginosa to worn lenses is consistent with the claim that it is the most implicated in all culture-positive contact lens related bacterial keratitis. Lens wear has different effects on bacterial adhesion, which may be due to type of lens materials and bacterial species/genera studied. Keywords: Silicone hydrogel lenses, conventional lenses, Pseudomonas aeruginosa, Staphylococcus aureus, bacterial adhesion.

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