scholarly journals Plant Extracts as Alternative Additives for Sperm Preservation

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 772
Author(s):  
José Luis Ros-Santaella ◽  
Eliana Pintus
Keyword(s):  
Plant Extracts ◽  
Animal Species ◽  
Sperm Quality ◽  
Antimicrobial Properties ◽  
Storage Conditions ◽  
Sperm Function ◽  
Fertilizing Ability ◽  
Semen Storage ◽  
Oxidative Damage To Proteins ◽  
Sperm Fertilizing Ability

Sperm preservation is a crucial factor for the success of assisted reproductive technology (ART) in humans, livestock, and wildlife. Irrespective of the extender and the storage conditions used, semen handling and preservation negatively affect sperm quality. Moreover, oxidative stress, which often arises during semen storage, significantly reduces sperm function and compromises the sperm fertilizing ability by inducing oxidative damage to proteins, lipids, and nucleic acids. Plant extracts have recently emerged as a cheap and natural source of additives to preserve and enhance sperm function during semen storage. The present work provides an update on the use of these natural compounds as alternative additives for sperm preservation in 13 animal species, including humans. A detailed description of the effects of 45 plant species, belonging to 28 families, on sperm function during semen storage is presented. The plant material and extraction method employed, dosage, possible toxic effects, and antimicrobial properties are provided.

Proteins from male and female reproductive tracts involved in sperm function regulation

Zygote ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Gabriela Hernández-Silva ◽  
Mayel Chirinos
Keyword(s):  
Reproductive Tract ◽  
Seminal Fluid ◽  
Female Reproductive Tract ◽  
Seminiferous Tubules ◽  
Sperm Function ◽  
Competent Cell ◽  
Male And Female ◽  
Fertilizing Ability ◽  
Sexually Mature ◽  
Sperm Fertilizing Ability

SummarySpermatogenesis is a dynamic process that culminates in the production of mature spermatozoa in the seminiferous tubules of sexually mature animals. Although sperm leaving the testis are fully differentiated, they must further undergo two additional maturation steps before acquiring the capability to fertilize the egg. Such processes take place during the epididymal residency and transport in the seminal fluid during ejaculation and, after delivery into the female reproductive tract, during the journey aiming the encountering the egg in the oviduct. Throughout this trip, spermatozoa are exposed to different reproductive fluids whose molecular compositions regulate the progress towards obtaining a fertilized competent cell. This review summarizes the evidence obtained so far supporting the participation of male and female reproductive tract-derived proteins in the modulation of sperm fertilizing ability and discusses the mechanisms by which such regulation may be accomplished.

scholarly journals Evaluation of sperm fertilizing ability using the Sperm Quality Analyzer

1997 ◽  
Vol 20 (2) ◽  
pp. 112-117 ◽  
Author(s):  
H. SHIBAHARA ◽  
S. NAITO ◽  
A. HASEGAWA ◽  
M. MITSUO ◽  
M. SHIGETA ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Fertilizing Ability ◽  
Quality Analyzer ◽  
Sperm Quality Analyzer ◽  
Sperm Fertilizing Ability

scholarly journals Pharmacological Inactivation of CatSper Blocks Sperm Fertilizing Ability Independently of the Capacitation Status of the Cells: Implications for Non-hormonal Contraception

2021 ◽  
Vol 9 ◽  
Author(s):  
Ludmila Curci ◽  
Guillermo Carvajal ◽  
Valeria Sulzyk ◽  
Soledad Natalia Gonzalez ◽  
Patricia S. Cuasnicú
Keyword(s):  
Hormonal Contraception ◽  
Hormonal Contraceptive ◽  
Sperm Function ◽  
Proof Of Concept ◽  
Fertilizing Ability ◽  
Mammalian Fertilization ◽  
First Time ◽  
Sperm Fertilizing Ability

Cation channel of sperm (CatSper), the main sperm-specific Ca2+ channel, plays a key role in mammalian fertilization, and it is essential for male fertility, becoming an attractive target for contraception. Based on this, in the present work, we investigated the effects of CatSper inactivation on in vitro and in vivo sperm fertilizing ability and the mechanisms underlying such effects. Exposure of cauda epididymal mouse sperm to different concentrations (1–20 μM) of the potent CatSper inhibitor HC-056456 (HC) during in vitro capacitation showed no effects on sperm viability but significantly affected Ca2+ entry into the cells, progressive motility, protein tyrosine phosphorylation, induced acrosome reaction, and hyperactivation, as well as the sperm’s ability to in vitro fertilize cumulus oocyte complexes and zona-free eggs. Whereas the presence of HC during gamete coincubation did not affect in vitro fertilization, exposure of either non-capacitating or already capacitated sperm to HC prior to gamete coincubation severely reduced fertilization, indicating that sperm function is affected by HC when the cells are incubated with the drug before sperm–egg interaction. Of note, insemination of HC-treated sperm into the uterus significantly or completely reduced the percentage of oviductal fertilized eggs showing, for the first time, the effects of a CatSper inhibitor on in vivo fertilization. These observations, together with the finding that HC affects sperm fertilizing ability independently of the sperm capacitation status, provide further insights on how CatSper regulates sperm function and represent a solid proof of concept for developing a male/female non-hormonal contraceptive based on the pharmacological blockage of CatSper activity.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

The Role of Epididymal Factors in Human Sperm Fertilizing Ability

1988 ◽  
Vol 541 (1 In Vitro Fert) ◽  
pp. 292-296 ◽  
Author(s):  
J. A. BLAQUIER ◽  
M. S. CAMEO ◽  
P. S. CUASNICU ◽  
M. F. GONZALEZ ECHEVERRIA ◽  
L. PINUEIRO ◽  
...  
Keyword(s):  
Human Sperm ◽  
Fertilizing Ability ◽  
Sperm Fertilizing Ability

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

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