Hydrogen Peroxide Increases during Endodormancy and Decreases during Budbreak in Grapevine (Vitis vinifera L.) Buds

Changes in the level of hydrogen peroxide (H2O2) is a good indicator to monitor fluctuations in cellular metabolism and in the stress responses. In this study, the changes in H2O2 content during bud endodormancy (ED) and budbreak were analysed in grapevine (Vitis vinifera L.). The results showed a gradual increase in the H2O2 content during the development of bud ED, which was mainly due to an increase in the activity of peroxidases (PODs). The maximum H2O2 content reached in the grapevine buds coincided with the maximum depth of bud ED. In contrast, during budbreak, the H2O2 content decreased. As the plant hormones cytokinin (CK) and auxin play an important role in budbreak and growth resumption in grapevine, the effect of exogenous applications of H2O2 on the expression of genes involved in CK and auxin metabolism was analysed. The results showed that H2O2 represses the expression of the CK biosynthesis genes VvIPT3a and VvLOG1 and induces the expression of the CK-inactivating gene VvCKX3, thus reducing potentially the CK content in the grapevine bud. On the other hand, H2O2 induced the expression of the auxin biosynthesis genes VvAMI1 and VvYUC3 and of the auxin transporter gene VvPIN3, thus increasing potentially the auxin content and auxin transport in grapevine buds. In general, the results suggest that H2O2 in grapevine buds is associated with the depth of ED and negatively regulates its budbreak.

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Genome-wide Analysis and Expression Profiling Suggest Diverse Roles of TCP Genes During Development and Stress Responses in Grapevine (Vitis vinifera L)

South African Journal of Enology and Viticulture ◽

10.21548/39-2-2769 ◽

2018 ◽

Vol 39 (2) ◽

Author(s):

Z Min ◽

Y Zhang ◽

R Li ◽

M Liu ◽

Y Ju ◽

...

Keyword(s):

Vitis Vinifera ◽

Stress Responses ◽

Expression Profiling ◽

Vitis Vinifera L ◽

Genome Wide Analysis ◽

Genome Wide

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Expression pattern of thaumatin in the selected red varieties of Vitis vinifera, L.

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1057 ◽

2019 ◽

Vol 13 (1) ◽

pp. 547-552

Author(s):

Jana Žiarovská ◽

Veronika Fialková ◽

Lucia Zamiešková ◽

Jana Bilčíková ◽

Lucia Zeleňáková ◽

...

Keyword(s):

Vitis Vinifera ◽

Stress Responses ◽

Lipid Transfer Protein ◽

Sweet Taste ◽

Vitis Vinifera L ◽

Cabernet Sauvignon ◽

Lipid Transfer ◽

Beneficial Effects ◽

A Minor ◽

Wine Region

Vitis vinifera L. is a specie that is adapted to a very variable range of climates, from cold up to the desert one, but especially it grows in the temperate Mediterranean regions and continental areas of Europe. Grape is a widespread consumed fruit as well as processed to musts, juices or wine. The health beneficial effects of grapes and wine are very well known due to their high nutritional value and unique phytochemical composition. Despite many health protective and beneficial effects of Vitis vinifera, a part of population suffer to allergic reactions to this fruit. Allergens of wine and grapes are: endochitinases, lipid-transfer protein and thaumatin. Thaumatin is a protein having a sweet taste belonging to the PR5-like proteins. These proteins are very difersified in their functions and were described to be involved in stress responses and fruit ripening, but are expressed in healthy grape fruits in a constitutive manner and needn´t to be expressed only as a answer to the stress. Thaumatin is a minor allergen in grape, but belonging to the suspected panallergens relevant to the food cross-allergy induction, its importance is quite high. Another importance of this protein is a technological one, as reported to aggregate in wine to form a visible haze unless removed prior to bottling. In this study, expression of thaumatin-like allergen was analysed in the grapes of selected varieties. Grapes of four red varieties of Vitis vinifera, L. were obtained in the season 2017 in the Sabo winery that belongs to the Malokarpatská wine region. Fresh maturated grapes of varieties Alibernet, Cabernet Sauvignon , Frankovka modrá and Dornfelder were analysed. Expression changes of thaumatin was calculated by delta delta Ct method. Dornfelder was found as to have the lowest activity in thaumatin-like gene activity, mainly when comparing to the Cabernet Sauvignon and Frankovka modrá. Alibernet, on the other side, has the expression level of thaumatin very similar when comparing to the Cabernet Sauvignon and Frankovka modrá.

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DIFFERENTIAL EXPRESSION OF GENES IN BERRIES OF CV. 'SANGIOVESE' (VITIS VINIFERA L.) DURING RIPENING FOLLOWING CLUSTER THINNING AT VÉRAISON

Acta Horticulturae ◽

10.17660/actahortic.2014.1046.60 ◽

2014 ◽

pp. 441-448

Author(s):

C. Pastore ◽

I. Filippetti ◽

S. Zenoni ◽

M. Fasoli ◽

A. Ferrarini ◽

...

Keyword(s):

Vitis Vinifera ◽

Differential Expression ◽

Vitis Vinifera L ◽

Expression Of Genes ◽

Cluster Thinning ◽

Differential Expression Of Genes

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Application of a framework for high resolution climate change impact assessment on grapevine (Vitis vinifera L.) at regional scale: Phenology, yield and biotic stress responses

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1307/6/37/372031 ◽

2009 ◽

Vol 6 (37) ◽

pp. 372031

Author(s):

Marco Moriondo ◽

M Bindi ◽

G Trombi

Keyword(s):

Climate Change ◽

High Resolution ◽

Vitis Vinifera ◽

Stress Responses ◽

Biotic Stress ◽

Climate Change Impact ◽

Regional Scale ◽

Vitis Vinifera L ◽

Climate Change Impact Assessment ◽

Change Impact

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On the role of H2O2 in the recovery of grapevine (Vitis vinifera cv. Prosecco) from Flavescence dorée disease

Functional Plant Biology ◽

10.1071/fp06308 ◽

2007 ◽

Vol 34 (8) ◽

pp. 750 ◽

Cited By ~ 52

Author(s):

Rita Musetti ◽

Rosita Marabottini ◽

Maurizio Badiani ◽

Marta Martini ◽

Luigi Sanità di Toppi ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Vitis Vinifera ◽

Membrane Lipids ◽

Thiobarbituric Acid ◽

Polymerase Chain Reaction Analysis ◽

Antioxidant Systems ◽

Vitis Vinifera L ◽

Flavescence Dorée ◽

Significant Difference ◽

Leaf Tissues

In the present work, we compared hydrogen peroxide (H2O2) localisation and the activities/contents of antioxidant enzymes and metabolites in the leaf tissues of grapevine (Vitis vinifera L. cv. Prosecco) plants showing different sanitary status, namely diseased by Flavescence dorée, healthy or recovered. Polymerase chain reaction analysis revealed that the pathogen associated with Flavescence dorée (proposed as ‘Candidatus Phytoplasma vitis’) was detected in the leaf tissues of symptomatic plants, but was not observed in either the healthy or recovered plants. Hydrogen peroxide accumulated in the phloem plasmalemma of recovered grapevine leaves, but was not detected in either healthy or diseased material. When compared to diseased or healthy plants, recovered plants had distinctly lower extractable levels of catalase and ascorbate peroxidase, two enzymes primarily involved in the scavenging of excess H2O2 generated in different cell compartments. Among healthy, diseased and recovered leaves there was no significant difference in the amount of 2-thiobarbituric acid-reactive substances, which are assumed to reflect the extent of peroxidative breakdown of membrane lipids. Therefore, it is suggested that recovery from Flavescence dorée disease in grapevine might be associated with a long-term, sustained and tissue-specific accumulation of H2O2 in leaves, which reduces numbers or prevents further infection by Flavescence dorée phytoplasma. Recovered grapevine plants might be able to achieve such H2O2 accumulation through a selective and presumably stable downregulation of enzymatic H2O2 scavengers, without altering the levels of other antioxidant systems and without incurring an increased oxidative risk.

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