Pearl Grey Shading Net Boosts the Accumulation of Total Carotenoids and Phenolic Compounds that Accentuate the Antioxidant Activity of Processing Tomato

Tomato (Solanum lycopersicum L.) is one of the most consumed vegetables worldwide due to its low caloric intake and high fiber, minerals, and phenolic compounds, making it a high-quality functional food. However, fruit quality attributes can be affected by pre-harvest factors, especially environmental stresses. This research aimed to evaluate the influence of two shading nets (white net −30% and pearl grey net −40% shading degree) on the yield and phytochemical profile of tomato fruits grown in summer under the Mediterranean climate. Mineral and organic acid content (by ion chromatography-IC), phenolic profile (by ultra-high performance liquid chromatography-UHPLC coupled with an Orbitrap high-resolution mass spectrometry-HRMS), carotenoid content (by high-performance liquid chromatography with diode array detection-HPLC-DAD), and antioxidant activities DPPH, ABTS, and FRAP (by UV-VIS spectrophotometry) were determined. Tomato fruits grown under the pearl grey net recorded the highest values of total phenolic compounds (14,997 µg 100 g−1 of fresh weight) and antioxidant activities DPPH, ABTS, and FRAP, without affecting either fruit color or marketable yield. The reduction of solar radiation through pearl grey nets proved to be an excellent tool to increase the phytochemical quality of tomato fruits during summer cultivation in a Mediterranean environment.

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Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽

10.3390/foods9101371 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1371 ◽

Cited By ~ 1

Author(s):

Vanesa Nuñez-Gómez ◽

Nieves Baenas ◽

Inma Navarro-González ◽

Javier García-Alonso ◽

Diego A. Moreno ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Methyl Jasmonate ◽

Bioactive Compounds ◽

High Performance ◽

Total Phenolic ◽

Diode Array ◽

Total Carotenoids ◽

Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

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Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽

10.3390/antiox9060488 ◽

2020 ◽

Vol 9 (6) ◽

pp. 488

Author(s):

Agnieszka Szopa ◽

Michał Dziurka ◽

Sebastian Granica ◽

Marta Klimek-Szczykutowicz ◽

Paweł Kubica ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Antioxidant Capacity ◽

Phenolic Acids ◽

High Performance ◽

Total Phenolic ◽

Array Detector ◽

Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

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Distribution of β-Glucan, Phenolic Acids, and Proteins as Functional Phytonutrients of Hull-Less Barley Grain

Foods ◽

10.3390/foods8120680 ◽

2019 ◽

Vol 8 (12) ◽

pp. 680 ◽

Cited By ~ 4

Author(s):

Gordana Šimić ◽

Daniela Horvat ◽

Alojzije Lalić ◽

Daliborka Koceva Komlenić ◽

Ivan Abičić ◽

...

Keyword(s):

Antioxidant Activity ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Protein Content ◽

Phenolic Acids ◽

High Performance ◽

Antioxidant Activities ◽

Barley Grain ◽

Total Phenolic ◽

Health Promoting

Two hull-less barley varieties were roller-milled, and breaks (B) and reduction flours (C), shorts, and bran were collected. Shorts, which mainly originate from endosperm cells with a smaller amount of the outer layers, had the largest yield (48.87–51.54%). Ash (0.82–3.10%) and protein (9.95–14.8%) increased from flours toward shorts and bran, while starch decreased (82.31–48.69%). In contrast to clear distribution differences in protein content (bran > shorts > C > B), albumins/globulins content was lowest in bran (0.78–0.90 g/100 gdw), and their distribution between fractions was uneven and genotype dependent. Distribution of hordeins (6.69–10.49 g/100 gdw) was more distinct and generally decreased in order from bran > B > shorts > C. The proportion of nutritionally poor C-hordeins in total hordeins varied from 28.33% to 30.24%, without significant differences between fractions. The β-glucan content varied from 0.80% to 7.49% with decreasing content in the order bran, shorts > C > B. Shorts and bran could be classified as moderate and high β-glucan flour (5.70–7.22%). The total phenolic and antioxidant activities ranged from 0.91 to 2.21 mg GAE/gdw and 28.81–72.06%, respectively. Ferulic and sinapic acids determined by high-performance liquid chromatography (HPLC) were major contributors to the antioxidant activity (45.16–1026.91 ug/gdw and 18.93–206.52 ug/gdw), respectively. The yield and high content of phytonutrients make hull-less barley shorts suitable for the production of health-promoting food and food supplements.

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LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Antioxidants ◽

10.3390/antiox10111770 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1770

Author(s):

Akhtar Ali ◽

Yasmeen M. Bashmil ◽

Jeremy J. Cottrell ◽

Hafiz A. R. Suleria ◽

Frank R. Dunshea

Keyword(s):

Liquid Chromatography ◽

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Positive Impact ◽

Total Phenolic ◽

Fragmentation Pattern ◽

Photodiode Array Detection ◽

Potential Health

Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.

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COMPARATIVE STUDY OF PHENOLIC COMPOUNDS COMPOSITION AND CONTENT IN LEAVES OF THE GE-NUS NITRARIA (NITRARIACEAE) PLANTS

chemistry of plant raw material ◽

10.14258/jcprm.2017041909 ◽

2017 ◽

pp. 107-116

Author(s):

Мария (Marija) Сергеевна (Sergeevna) Воронкова (Voronkova) ◽

Евгений (Evgenij) Викторович (Viktorovich) Банаев (Banaev) ◽

Мария (Marija) Анатольевна (Anatol'evna) Томошевич (Tomoshevich)

Keyword(s):

High Performance Liquid Chromatography ◽

Multivariate Analysis ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Seasonal Dynamics ◽

High Performance ◽

Total Phenolic ◽

Total Phenolic Compounds ◽

Flowering Stage ◽

Study Results

The content and composition of phenolic compounds in leaves of 3 Nitraria L. species from 12 populations have been studied with a high-performance liquid chromatography (HPLC). The investigation has detected 17 phenolic compounds, the maximum number of components is 12 indicated in leaves of N. pamirica L. Vassil. and N. schoberi L. plants in 2 populations, the minimum – 6 components – has been revealed in leaves of N. komarovii Iljin et Lava. Hyperoside (O-glycoside of quercetin), narcissin (O-glycoside of isoramnetin), quercetin (flavonol) and luteolin (flavon) have been identified. Studied plants accumulate a significant amount of phenolic compounds. Their content reaches 2,57% in leaves of N. schoberi L., 3,96% in N. komarovii. It was marked seasonal dynamics of a number of substances, in particular, lower narcissin content during plants fruiting compared to a flowering stage. The study results allow making an assumption of species peculiarities of phenolic compounds composition and content in N. schoberi and N. komarovii. The components composition is poorer in extracts of N. komarovii leaves, however, they shows higher content of total phenolic compounds compared with N. schoberi plants. At the same time N. pamirica sample is close to N. schoberi one based on a multivariate analysis of phenolic compounds composition and content.

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