scholarly journals Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 191 ◽  
Author(s):  
Saleha Akter ◽  
Rama Addepalli ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Plant Extracts ◽  
Direct Reduction ◽  
Proliferation Assay ◽  
Free System ◽  
Cell Proliferation Assay ◽  
A Cell ◽  
The Impact ◽  
Terminalia Ferdinandiana

The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes. The CellTiter 96® AQueous One Solution Cell Proliferation Assay (CellTiter) was used to measure cell viability in response to antioxidant-rich extracts of Terminalia ferdinandiana fruits. Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell viability compared to lower concentrations. Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay reagent. To confirm this effect in a similar cell proliferation assay, we employed the CellTiter-Blue® Cell Viability Assay and again observed increased viability with increased concentrations of the extracts and direct reduction of the assay reagent by the extracts in cell-free systems. In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the CyQUANT® NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. Cell viability decreased with increasing concentrations of the extracts. Accordingly, the results of the present study indicated that cell viability assays reliant upon dehydrogenase activity may lead to false positive results when testing antioxidant-rich plant extracts with intrinsic reductive potential, and alternative cell viability assays should be used to measure the cell viability.

A cell proliferation assay for small fish and aquatic invertebrates using bath exposure to bromodeoxyuridine

1994 ◽  
Vol 30 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Michael J. Moore ◽  
Dale F. Leavitt ◽  
Alice M. Shumate ◽  
Philip Alatalo ◽  
John J. Stegeman
Keyword(s):  
Cell Proliferation ◽  
Aquatic Invertebrates ◽  
Small Fish ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
A Cell

scholarly journals Proliferation Effects on Hair Growth of Compounds Isolated from the Bark of Dalbergia oliveri

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Seon Ju Park ◽  
Nguyen Xuan Nhiem ◽  
Bui Huu Tai ◽  
Hoang Le Tuan Anh ◽  
Seok Hyun Oh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hair Growth ◽  
Spectroscopic Data ◽  
Dermal Papilla ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Proliferation Assay ◽  
Dermal Papilla Cells ◽  
Cell Proliferation Assay ◽  
A Cell

One new isoflavane, 7,4′-dihydroxy-isoflavanquinone (1), together with ten known compounds (2–11) were isolated from the bark of Dalbergia oliveri Prain. The structures of compounds were determined on the basis of extensive spectroscopic methods, including 1D and 2D NMR and CD spectroscopic data. Using a cell proliferation assay, the isolated compounds were evaluated for their proliferation effects on hair growth. (3 R)-5’-Methoxyvestitol (2) and (6a R,11a R)-3,8-dihydroxy-9-methoxypterocarpan (10) significantly increased the proliferation of immortalized dermal papilla cells (iDPC).

scholarly journals The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Zhengbing Lv ◽  
Tao Wang ◽  
Wenhua Zhuang ◽  
Dan Wang ◽  
Jian Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Bombyx Mori ◽  
Expression Patterns ◽  
Ras Oncogene ◽  
Proliferation Assay ◽  
Cytoplasmic Staining ◽  
Cell Proliferation Assay ◽  
Silkworm Pupae ◽  
A Cell

The Ras oncogene of silkworm pupae (Bras2) may belong to the Ras superfamily. It shares 77% of its amino acid identity with teratocarcinoma oncogene 21 (TC21) related ras viral oncogene homolog-2 (R-Ras2) and possesses an identical core effector region. The mRNA ofBombyx moriBras2 has 1412 bp. The open reading frame contains 603 bp, which encodes 200 amino acid residues. This recombinant BmBras2 protein was subsequently used as an antigen to raise a rabbit polyclonal antibody. Western blotting and real-time PCR analyses showed that BmBras2 was expressed during four developmental stages. The BmBras2 expression level was the highest in the pupae and was low in other life cycle stages. BmBras2 was expressed in all eight tested tissues, and it was highly expressed in the head, intestine, and epidermis. Subcellular localization studies indicated that BmBras2 was predominantly localized in the nuclei of Bm5 cells, although cytoplasmic staining was also observed to a lesser extent. A cell proliferation assay showed that rBmBras2 could stimulate the proliferation of hepatoma cells. The higher BmBras2 expression levels in the pupal stage, tissue expression patterns, and a cell proliferation assay indicated that BmBras2 promotes cell division and proliferation, most likely by influencing cell signal transduction.

scholarly journals Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay

2021 ◽  
Vol 18 (12) ◽  
pp. 6254
Author(s):  
Rehab Elfadul ◽  
Roman Jesien ◽  
Ahmed Elnabawi ◽  
Paulinus Chigbu ◽  
Ali Ishaque
Keyword(s):  
Cell Proliferation ◽  
Estrogenic Activity ◽  
Landing Site ◽  
Negative Control ◽  
Proliferation Assay ◽  
Negative Effects ◽  
Cell Proliferation Assay ◽  
Coastal Bays ◽  
Maryland Coastal Bays ◽  
Mcf 7

Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses.

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