scholarly journals Proliferation Effects on Hair Growth of Compounds Isolated from the Bark of Dalbergia oliveri

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Seon Ju Park ◽  
Nguyen Xuan Nhiem ◽  
Bui Huu Tai ◽  
Hoang Le Tuan Anh ◽  
Seok Hyun Oh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hair Growth ◽  
Spectroscopic Data ◽  
Dermal Papilla ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Proliferation Assay ◽  
Dermal Papilla Cells ◽  
Cell Proliferation Assay ◽  
A Cell

One new isoflavane, 7,4′-dihydroxy-isoflavanquinone (1), together with ten known compounds (2–11) were isolated from the bark of Dalbergia oliveri Prain. The structures of compounds were determined on the basis of extensive spectroscopic methods, including 1D and 2D NMR and CD spectroscopic data. Using a cell proliferation assay, the isolated compounds were evaluated for their proliferation effects on hair growth. (3 R)-5’-Methoxyvestitol (2) and (6a R,11a R)-3,8-dihydroxy-9-methoxypterocarpan (10) significantly increased the proliferation of immortalized dermal papilla cells (iDPC).

A cell proliferation assay for small fish and aquatic invertebrates using bath exposure to bromodeoxyuridine

1994 ◽  
Vol 30 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Michael J. Moore ◽  
Dale F. Leavitt ◽  
Alice M. Shumate ◽  
Philip Alatalo ◽  
John J. Stegeman
Keyword(s):  
Cell Proliferation ◽  
Aquatic Invertebrates ◽  
Small Fish ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
A Cell

scholarly journals miR-140-5p in Small Extracellular Vesicles From Human Papilla Cells Stimulates Hair Growth by Promoting Proliferation of Outer Root Sheath and Hair Matrix Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Yuxin Chen ◽  
Junfei Huang ◽  
Zhen Liu ◽  
Ruosi Chen ◽  
Danlan Fu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Extracellular Vesicles ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Luciferase Reporter ◽  
Outer Root Sheath ◽  
Dermal Papilla Cells ◽  
Root Sheath ◽  
Hair Matrix ◽  
Low Passage

The application of dermal papilla cells to hair follicle (HF) regeneration has attracted a great deal of attention. However, cultured dermal papilla cells (DPCs) tend to lose their capacity to induce hair growth during passage, restricting their usefulness. Accumulating evidence indicates that DPCs regulate HF growth mainly through their unique paracrine properties, raising the possibility of therapies based on extracellular vesicles (EVs). In this study, we explored the effects of EVs from high- and low-passage human scalp follicle dermal papilla cells (DP-EVs) on activation of hair growth, and investigated the underlying mechanism. DP-EVs were isolated by ultracentrifugation and cultured with human scalp follicles, hair matrix cells (MxCs), and outer root sheath cells (ORSCs), and we found low-passage DP-EVs accelerated HF elongation and cell proliferation activation. High-throughput miRNA sequencing and bioinformatics analysis identified 100 miRNAs that were differentially expressed between low- (P3) and high- (P8) passage DP-EVs. GO and KEGG pathway analysis of 1803 overlapping target genes revealed significant enrichment in the BMP/TGF-β signaling pathways. BMP2 was identified as a hub of the overlapping genes. miR-140-5p, which was highly enriched in low-passage DP-EVs, was identified as a potential regulator of BMP2. Direct repression of BMP2 by miR-140-5p was confirmed by dual-luciferase reporter assay. Moreover, overexpression and inhibition of miR-140-5p in DP-EVs suppressed and increased expression of BMP signaling components, respectively, indicating that this miRNA plays a critical role in hair growth and cell proliferation. DP-EVs transport miR-140-5p from DPCs to epithelial cells, where it downregulates BMP2. Therefore, DPC-derived vesicular miR-140-5p represents a therapeutic target for alopecia.

scholarly journals Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 191 ◽  
Author(s):  
Saleha Akter ◽  
Rama Addepalli ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Plant Extracts ◽  
Direct Reduction ◽  
Proliferation Assay ◽  
Free System ◽  
Cell Proliferation Assay ◽  
A Cell ◽  
The Impact ◽  
Terminalia Ferdinandiana

The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes. The CellTiter 96® AQueous One Solution Cell Proliferation Assay (CellTiter) was used to measure cell viability in response to antioxidant-rich extracts of Terminalia ferdinandiana fruits. Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell viability compared to lower concentrations. Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay reagent. To confirm this effect in a similar cell proliferation assay, we employed the CellTiter-Blue® Cell Viability Assay and again observed increased viability with increased concentrations of the extracts and direct reduction of the assay reagent by the extracts in cell-free systems. In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the CyQUANT® NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. Cell viability decreased with increasing concentrations of the extracts. Accordingly, the results of the present study indicated that cell viability assays reliant upon dehydrogenase activity may lead to false positive results when testing antioxidant-rich plant extracts with intrinsic reductive potential, and alternative cell viability assays should be used to measure the cell viability.

scholarly journals The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Zhengbing Lv ◽  
Tao Wang ◽  
Wenhua Zhuang ◽  
Dan Wang ◽  
Jian Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Bombyx Mori ◽  
Expression Patterns ◽  
Ras Oncogene ◽  
Proliferation Assay ◽  
Cytoplasmic Staining ◽  
Cell Proliferation Assay ◽  
Silkworm Pupae ◽  
A Cell

The Ras oncogene of silkworm pupae (Bras2) may belong to the Ras superfamily. It shares 77% of its amino acid identity with teratocarcinoma oncogene 21 (TC21) related ras viral oncogene homolog-2 (R-Ras2) and possesses an identical core effector region. The mRNA ofBombyx moriBras2 has 1412 bp. The open reading frame contains 603 bp, which encodes 200 amino acid residues. This recombinant BmBras2 protein was subsequently used as an antigen to raise a rabbit polyclonal antibody. Western blotting and real-time PCR analyses showed that BmBras2 was expressed during four developmental stages. The BmBras2 expression level was the highest in the pupae and was low in other life cycle stages. BmBras2 was expressed in all eight tested tissues, and it was highly expressed in the head, intestine, and epidermis. Subcellular localization studies indicated that BmBras2 was predominantly localized in the nuclei of Bm5 cells, although cytoplasmic staining was also observed to a lesser extent. A cell proliferation assay showed that rBmBras2 could stimulate the proliferation of hepatoma cells. The higher BmBras2 expression levels in the pupal stage, tissue expression patterns, and a cell proliferation assay indicated that BmBras2 promotes cell division and proliferation, most likely by influencing cell signal transduction.

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