scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 248 ◽  
Author(s):  
Ivana Savic Gajic ◽  
Ivan Savic ◽  
Ivana Boskov ◽  
Stanko Žerajić ◽  
Ivana Markovic ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Plant Material ◽  
Phenolic Content ◽  
Black Locust ◽  
Extraction Time ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers using central composite design. The ethanol concentration (33–67%), extraction temperature (33–67 °C), and extraction time (17–33 min) were analyzed as the factors that impact the total phenolic content. The liquid-to-solid ratio of 10 cm3 g−1 was the same during extractions. The optimal conditions were found to be 59 °C, 60% (v/v) ethanol, and extraction time of 30 min. The total phenolic content (TPC = 3.12 gGAE 100 g−1 dry plant material) and antioxidant activity (IC50 = 120.5 µg cm−3) of the extract obtained by ultrasound-assisted extraction were compared with those obtained by maceration (TPC = 2.54 gGAE 100 g−1 dry plant material, IC50 = 150.6 µg cm−3) and Soxhlet extraction (TPC = 3.22 gGAE 100 g−1 dry plant material, IC50 = 204.2 µg cm−3). The ultrasound-assisted extraction gave higher total phenolic content and better antioxidant activity for shorter extraction time so that it represents the technique of choice for the extraction of phenolic compounds. The obtained extract, as the source of antioxidants, can be applied in the pharmaceutical and food industries.

scholarly journals Ultrasound-Assisted Extraction of Antioxidants from Baccharis dracunculifolia and Green Propolis

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1530
Author(s):  
Renata Iara Cavalaro ◽  
Luis Felipe de Freitas Fabricio ◽  
Thais Maria Ferreira de Souza Vieira
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Southeastern Brazil ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Baccharis dracunculifolia or rosemary-of-field is the principal botanical source used by Africanized bees Apis mellifera L. to produce green propolis in Southeastern Brazil. The phenolic compounds present in the plant and green propolis have been reported to be responsible for biological activities such as antioxidant capacity. This study aimed to optimize the ultrasound-assisted extraction of antioxidants compounds from rosemary-of-field using a central composite rotatable design (CCRD), and compare results to green propolis extract. An experimental design was performed to obtain responses of total phenolic content and antioxidant capacity. The results allowed observing that the optimum condition for both Baccharis dracunculifolia floral bud and raw green propolis antioxidant extraction was obtained with 99% ethanol solution. In this condition, Total Phenolic Content (TPC), Ferric Reducing Antioxidant Power (FRAP), and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) values were 612.14 mg GAE. g−1 sample, 534.39 µM ferrous sulfate g−1 sample, and 72.37 µmol TEAC. g−1 sample, respectively, for floral buds. These results have confirmed that optimization is a key step for effective and sustainable extraction processes to be feasible on an industrial scale. The proposed process can be easily adopted on a larger scale, as it uses very few inputs and presents straightforward steps, with the advantage of high efficiency in the extraction of phenolic compounds from the studied matrices compared to the results from the literature. The high concentration of antioxidants indicates that the products obtained can be considered as a sustainable bioactive source for food and cosmetic purposes.

Optimization of Total Phenol and Tannin Content and Biological Activity of Dittrichia graveolens (L.) GREUTER.

2020 ◽  
Vol 16 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Mahsa Souri ◽  
Alireza Shakeri
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Methanol Concentration ◽  
Total Phenolic ◽  
Tannin Content ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.

scholarly journals Exhausted Olive Pomace Phenolic-Rich Extracts Obtention: A First Step for a Biorefinery Scheme Proposal

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 10
Author(s):  
Irene Gómez-Cruz ◽  
Inmaculada Romero ◽  
María del Mar Contreras ◽  
Carmen Padilla-Rascón ◽  
Florbela Carvalheiro ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Added Value ◽  
Total Phenolic ◽  
Olive Pomace ◽  
Residual Oil ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The exhausted olive pomace (EOP) is the main residue generated year-round after the extraction of the residual oil contained in the olive pomace. Due to its chemical composition, EOP is an interesting bioresource for the production of bioenergy and high added-value products. Therefore, the purpose of this study was to evaluate the extraction of antioxidants from EOP using sequential ultrasound-assisted extraction (UAE) and maceration with constant stirring. Water and 70% acetone were used as solvents. The total phenolic content was determined by the Folin–Ciocalteu method, the antioxidant capacity was investigated and the phenolic extracts were also characterized.

scholarly journals A Sustainable Approach for Extracting Non-Extractable Phenolic Compounds from Mangosteen Peel Using Ultrasound-Assisted Extraction and Natural Deep Eutectic Solvents

2021 ◽  
Vol 11 (12) ◽  
pp. 5625
Author(s):  
Merichel Plaza ◽  
Gloria Domínguez-Rodríguez ◽  
Cristina Sahelices ◽  
María Luisa Marina
Keyword(s):  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Extraction Time ◽  
Size Exclusion ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Mangosteen Peel ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasound Assisted

Mangosteen (Garcinia mangostana L.) peel is a potential source of phenolic compounds with beneficial properties. Natural deep eutectic solvents (NaDES) have been considered an environmentally friendly and cheap alternative to conventional organic solvents. In this work, a green extraction methodology was developed using ultrasound-assisted extraction (UAE) and NaDES for the extraction of antioxidant non-extractable polyphenols (NEPs) from mangosteen peel. To select the best NaDES to extract NEPs from mangosteen peel, seven NaDES were studied. Antioxidant capacity and total phenolic and proanthocyanidin contents were determined for the extracts. The molecular weights for the NEPs present in those extracts were evaluated by size exclusion chromatography. Experimental results showed that choline chloride–lactic acid (1:2) was the NaDES allowing the highest antioxidant proanthocyanidin content in the extracts. A Box–Behnken experimental design was employed to optimize the main parameters in UAE with NaDES: water percentage, ultrasound amplitude, and extraction time. The optimal extraction conditions were 18.8% (v/v) water, 60% ultrasound amplitude, and 15 min as the extraction time. In addition, the cytotoxicity of the NEP extracts obtained under optimal extraction conditions was evaluated. Results indicated for the first time that the use of NaDES in combination with UAE could be a sustainable alternative for the extraction of antioxidant NEPs from mangosteen peel for important applications in the food, pharmaceutical, agrochemical, and cosmetic fields, as the extracts presented low cytotoxicity.

scholarly journals The influence of extraction method on antioxidant potential of Tilia argentea flowers and bracts

2020 ◽  
Vol 31 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Serap Ayaz Seyhan ◽  
Zümrüt Yilmaz ◽  
Deniz Çikla Yilmaz
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe objective of this work was to compare the extraction of phenolic compounds from Tilia argentea flowers and bracts by using conventional (solvent extraction) and novel (ultrasound assisted) extraction methods. Ethanol (70 %) extracts were analyzed for their antioxidant activities. Total phenolic content was determined using Folin-Ciocalteu method and the antioxidant potential was determined by DPPH radical scavenging and Ferric Reducing Antioxidant Power (FRAP) assays. To determine the effect of ultrasound treatment on the extraction, same extraction parameters were applied in both methods. The results showed that extracts obtained by ultrasound assisted extraction have higher total phenolic content and antioxidant activity.

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Optimization of Ultrasound-Assisted Extraction via Sonotrode of Phenolic Compounds from Orange By-Products

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1120
Author(s):  
María del Carmen Razola-Diaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Phenolic Content ◽  
Orange Peel ◽  
Polar Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel is the main by-product from orange juice industry. It is a known source of bioactive compounds, mostly phenolic compounds, and it has been widely studied for its healthy activities. Thus, this research focuses on the establishment of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode. For this purpose, a Box–Behnken design of 27 experiments was carried out with four independent factors—ratio ethanol/water (v/v), time (min), amplitude (%), and pulse (%). Quantitative analyses of phenolic compounds were performed and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology (RSM). The extracts obtained in the established conditions were analyzed by High Performance Liquid Chromatography (HPLC) coupled to mass spectrometer detector and 74 polar compounds were identified. The highest phenolic content and antioxidant activity were obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W), and pulse 100%. The established method allows an increment of phenolics recovery up to 60% higher than a conventional extraction. Moreover, the effect of drying on phenolic content was also evaluated.

Sign in / Sign up

Export Citation Format

Share Document