scholarly journals Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 110
Author(s):  
Brisamar Estébanez ◽  
Alexandra L. Rodriguez ◽  
Nishant P. Visavadiya ◽  
Michael Whitehurst ◽  
María J. Cuevas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aerobic Training ◽  
Oxidative Stress Response ◽  
Pentraxin 3 ◽  
Elderly Adults ◽  
Nitrogen Species ◽  
Toll Like Receptor ◽  
Oxidative Stress Biomarkers ◽  
Toll Like Receptor 4 ◽  
Stress Biomarkers

Reactive oxygen and nitrogen species-mediated cellular aging has been linked to diseases such as atherothrombosis and cancer. Although pentraxin 3 (PTX3) is associated with aging-related diseases via TLR4-dependent anti-inflammatory effects, its relationship with oxidative stress in aging remains to be elucidated. Exercise is proposed as the key intervention for health maintenance in the elderly. This study aimed to examine the association of PTX3 levels with changes in oxidative stress in both plasma and peripheral blood mononuclear cells (PBMCs), following aerobic training in elderly adults. Nine trained and five controls participated in an eight-week aerobic training protocol. Enzyme-linked immunosorbent assay (ELISA) and Western blot analyses were used to determine PTX3, toll-like receptor 4 (TLR4), and levels of oxidative stress biomarkers [3-nitrotyrosine (3NT), 4-hydroxynonenal (4-HNE), glutathione (GSH), protein carbonyl (PC), reactive oxygen/ nitrogen species (ROS/RNS), and trolox equivalent antioxidant capacity (TEAC)] in plasma and/or PBMCs. Results showed a down-regulation of PTX3 expression in PBMCs following aerobic training, along with decreased PTX3/TLR4 ratios. Oxidative stress responses in PBMCs remained unchanged with the exercise protocol. Comparable levels of plasma PTX3 and oxidative stress biomarkers were observed in trained vs. control groups. No correlation was found between PTX3 and any oxidative stress biomarkers following training. These findings demonstrated the down-regulation of PTX3 and PTX3/TLR4 ratio, irrespective of oxidative stress response, in elderly adults following eight weeks of aerobic training.

scholarly journals Effects Of Aerobic Training On Pentraxin 3/Toll-like Receptor 4 And Oxidative Status In Elderly Adults

2020 ◽  
Vol 52 (7S) ◽  
pp. 475-475
Author(s):  
Shawn Dinh ◽  
Brisamar Estebanez ◽  
Alexandra A. Rodriguez ◽  
Nishant P. Visavadiya ◽  
Maria J. Cuevas ◽  
...  
Keyword(s):  
Aerobic Training ◽  
Oxidative Status ◽  
Pentraxin 3 ◽  
Elderly Adults ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals Effects of an aerobic training program on oxidative stress biomarkers in bulls

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 422-428 ◽  
Author(s):  
BM Escribano ◽  
I. Tunez ◽  
F. Requena ◽  
MD Rubio ◽  
R. De Miguel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Exercise Training ◽  
Training Program ◽  
Aerobic Training ◽  
Oxidative Stress Biomarkers ◽  
Enzymatic Antioxidant ◽  
Stress Biomarkers ◽  
Training Protocol

The aim of this study was to evaluate the effect of aerobic training (16 weeks – T1 and 24 weeks – T2) on oxidative stress biomarkers. To this end, GSH, GSH-peroxidase (GSH-Px) and catalase (CAT) activity were analysed in plasma. Nine bulls (3–4 years), were included in this work. The exercise training protocol was performed in a track ("taurodromo") three days per week for 24 weeks and consisted of 400 m warming up, 1200 m to 4–5 m/s, two minutes' resting, 1200 m to 4-5 m/s and, finally, 400 m walking. The results reflected that GSH-Px activity was higher at T1 (6.18 ± 0.45) than at baseline (T0; 2.31 ± 0.08) while the GSH level (2.98 ± 0.37) was lower vs. T0 (14.59 ± 3.40). Moreover, there were significant increases in GSH-Px (18.23 ± 1.36) and CAT (2.52 ± 0.04) activities and the recovery of basal values in GSH (11.75 ± 2.84) in T2. In conclusion, the type of training carried out in this study involved two well-defined stages: (i) a period of perturbation, followed by (ii) adaptation. The former stage was characterised by the induction of oxidative stress manifested as a decrease in the GSH, and the latter (T2) by the recovery of this non-enzymatic antioxidant.

scholarly journals Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3167
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Daniela Pigini ◽  
Giovanna Tranfo ◽  
Silvia Canepari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Occupational Exposure ◽  
Oxidation Products ◽  
Control Group ◽  
Welding Fumes ◽  
Oxidative Stress Biomarkers ◽  
Limit Values ◽  
Stress Biomarkers ◽  
Specificity And Sensitivity ◽  
The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

scholarly journals Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Muscle Tissue ◽  
Salmo Trutta ◽  
Protein Carbonyl ◽  
Oxidative Modification ◽  
Oxidative Stress Biomarkers ◽  
Development Stages ◽  
Stress Biomarkers ◽  
Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

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