scholarly journals Development of Multifunctional Cosmetic Cream Using Bioactive Materials from Streptomyces sp. T65 with Synthesized Mesoporous Silica Particles SBA-15

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 278
Author(s):  
Ram Hari Dahal ◽  
Tuan Manh Nguyen ◽  
Dong Seop Shim ◽  
Joon Young Kim ◽  
Jangyul Lee ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Type I ◽  
Mushroom Tyrosinase ◽  
Dpph Radical ◽  
Crude Product ◽  
Dpph Radical Scavenging

Various cosmetics having a single function are increasingly being used, but cosmetics having multifunctional activities remain limited. We aimed to develop a multifunctional cosmetic cream having antioxidant, anti-tyrosinase, anti-aging and antimicrobial activities. Antimicrobial activities were performed by disc-diffusion method. Cell toxicity and cell proliferations were evaluated in a 96-well plate with different cell lines such as HaCaT, RAW264.7, CCD-986Sk, B16F1, and B16F10. Mushroom tyrosinase inhibition, elastase inhibition, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were evaluated and IC50 was calculated. Mesoporous silica particle was synthesized using Pluronic P123 and tetraethyl ortho-silicate (TEOS). Facial pictures were captured by VISIA-CR (Facial Imaging System for Clinical Research). Roughness of image was analysed by PRIMOS software and brightness of image was analyzed by Chromameter CR-400. The crude product of strain T65 inhibited the different human pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Propionibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The IC50 of T65 crude product for mushroom tyrosinase, elastase, and DPPH radical scavenging activities were 58.73, 14.68, and 6.31 µg/mL, respectively. T65 crude product proliferated collagen type I in CCD-986Sk cell up to 145.91% ± 9.11% (mean ± SD; mean of 24, 48, and 72 h) at 250 pg/mL. Synthesized mesoporous particles (SBA-15) confirmed the sustainable performance by control-release for three days. Formulated functional cosmetic cream containing T65 embedded SBA-15, significantly decreased the skin roughness by 4.670% and increased the skin brightness by 0.472% after application of 4 weeks. T65 crude product inhibited both Gram-positive and Gram-negative pathogens. Synthesized mesoporous particle, SBA-15, confirmed the physiologically active substance was released in sustainable release condition. T65 crude product showed impeccable antimicrobial, antioxidant, anti-aging, and whitening activities with non-cytotoxic effects to different cell lines related to the human skin.

scholarly journals Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde

2021 ◽  
Vol 9 (10) ◽  
pp. 1915-1920
Author(s):  
Şükrü Canpolat ◽  
Ilgaz Akata ◽  
Yasemin İşlek ◽  
Elif Canpolat ◽  
Cemil İşlek
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Morchella Esculenta ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.

Antimicrobial activities of Allium staticiforme and Allium subhirsutum

2020 ◽  
Vol 15 (1) ◽  
pp. 19-23
Author(s):  
Alican Bahadir Semerci ◽  
Dilek İnceçayır ◽  
Vusale Mammadova ◽  
Ayşegül Hoş ◽  
Kenan Tunç
Keyword(s):  
Radical Scavenging ◽  
Activity Level ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Antifungal Activities ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Dpph Radical Scavenging

The antibacterial and antifungal activities of the bulb and flower of Allium staticiforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAE/100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities. Video Clip of Methodology: Disc Diffusion Method: 3 min 04 sec:  Full Screen   Alternate

scholarly journals Isolation, Characterization and Antioxidant, Tyrosinase Inhibitory Activities of Constituents from the Flowers of Cercis glabra ‘Spring-1’

2021 ◽  
Vol 15 (4) ◽  
pp. 254-260
Author(s):  
Penghua Shu ◽  
Yamin Li ◽  
Yuehui Luo ◽  
Shujing Cai ◽  
Yingying Fei ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Mushroom Tyrosinase ◽  
Dpph Radical ◽  
Inhibitory Effects ◽  
Isolation And Identification ◽  
Phytochemical Study ◽  
Inhibitory Activities ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

A phytochemical study on the flowers of Cercis glabra ‘Spring-1’ led to the isolation and identification of twelve compounds, including one new compound named as 1-O-α-l-rhamnosyl-(E)-phytol (1) and eleven known compounds. Their structures were elucidated based on physical data analysis, including HR-ESI-MS, NMR, UV, IR, and acid hydrolysis. All compounds were screened for in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl method. Compounds 4 and 5 exhibited obvious DPPH radical scavenging activities. All the isolates were tested for their inhibitory effects on mushroom tyrosinase, and compounds 6, 7, 10 and 11 showed moderate tyrosinase inhibitory activities.

scholarly journals Usefulness of Urea as a Means of Improving the Solubility of Poorly Water-Soluble Ascorbyl Palmitate

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Yutaka Inoue ◽  
Daichi Niiyama ◽  
Isamu Murata ◽  
Ikuo Kanamoto
Keyword(s):  
Radical Scavenging ◽  
Ascorbyl Palmitate ◽  
Water Soluble ◽  
Mushroom Tyrosinase ◽  
Inhibition Assay ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Solubility Test ◽  
Crystal System ◽  
Dpph Radical Scavenging

The aim of this study was to evaluate complexes of L-ascorbyl palmitate (ASCP) and urea (UR). This evaluation involved differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), scanning electron microscopy (SEM), near-infrared spectroscopy (NIR), a solubility test, a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test, and a mushroom tyrosinase inhibition assay. Physicochemical evaluation revealed that ASCP/UR complexes form at a molar ratio of 1/12. The solubility test revealed that ASCP/UR complexes had increased solubility compared to ASCP. The DPPH radical scavenging test and mushroom tyrosinase inhibition assay revealed that the activity of ASCP/UR complexes was not impaired by complex formation. These results are probably due to the tetragonal crystal system of UR changing to a hexagonal crystal system and interaction with the alkyl group of ASCP.

scholarly journals Chemical Composition, Antioxidant, and Antimicrobial Activities of Essential Oils of Endlicheria arenosa (Lauraceae) from the Amazon

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Joyce Kelly R. da Silva ◽  
Rafaela C. S. da Trindade ◽  
José Guilherme S. Maia ◽  
William N. Setzer
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Dpph Radical ◽  
Volatile Oils ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Dpph Radical Scavenging

The essential oils (EOs) of leaves and twigs of Endlicheria arenosa Chanderb. were obtained by hydrodistillation using a Clevenger-type apparatus and the chemical composition was determined by GC and GC-MS. In total, 47 constituents were identified and sesquiterpene hydrocarbons (77.6%) were the main compounds found in the leaf EO, such as bicyclogermacrene (42.2%), germacrene D (12.5%) and β-caryophyllene (10.1%). Limonene (33.2%), terpinen-4-ol (15.6%) and δ-cadinol (6.9%) were the predominant constituents in the EO of the twigs. Using the DPPH radical-scavenging method, the EO of the leaves showed the greater radical-scavenging activity (216.5 ± 11.6 mg Trolox equivalents (TE)/mL compared to the EO of the twigs (122.6 ± 6.8 mg TE/mL). Also, the EO of leaves displayed promising antimicrobial activity against Escherichia coli (MIC 19.5 μg•mL−1). The present study is the first report about evaluation of volatile oils for this species.

scholarly journals Antioxidant and cytotoxic activities of Artemisia monosperma L. and Tamarix aphylla L. essential oils

2021 ◽  
Vol 49 (1) ◽  
pp. 12233
Author(s):  
Ramy M. ROMEILAH ◽  
Hossam S. EL-BELTAGI ◽  
Emad A. SHALABY ◽  
Kareem M. YOUNES ◽  
Hani EL MOLL ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Principal Component ◽  
Cytotoxic Agents ◽  
Promyelocytic Leukaemia ◽  
Dpph Radical ◽  
Leukaemia Cell ◽  
Dpph Radical Scavenging

Essential (volatile) oil from leaves of Artemisia monosperma L. belonging to family Asteraceae, and aerial parts of Tamarix aphylla L. (Athel) belonging to family Tamaricaceae were collected from the desert of Ha'il region, northern region of Saudi Arabia, hydro distilled by Clevenger apparatus and analysed by means of GC-MS techniques. Antioxidant activities of essential oils of A. monosperma and T. aphylla compared with ascorbic acid and butylated hydroxytoluene (BHT) as reference antioxidant compound were determined by method of DPPH radical scavenging assay and ABTS assay. In vitro screening of potential cytotoxicity of essential oils was also evaluated against human promyelocytic leukaemia cell lines (HL60 and NB4). The GC/MS analysis of A. monosperma essential oil resulted in identification of 61 components predominated mainly by β-Pinene as principal component (29.87%) and T. aphylla resulted in identification of 37 components of essential oil predominated mainly by 6,10,14- trimethyl-2-pentadecanone (21.43%) as principal component. Antioxidant activity as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) increased with increasing essential oil concentrations of A. monosperma and T. aphylla (25, 50, 75, 100 and 200 μg mL-1). The most pronounced increases detected in the high concentrations of the two essential oils. Biologically, essential oil extracts exhibited cytotoxicity effects in dose dependent manner against human promyelocytic leukaemia cell lines (HL60 and NB4). In conclusion, A. monosperma and T. aphylla essential oils could be valuable source for cytotoxic agents with high safety and selective cytotoxicity profiles.

scholarly journals Enhancement of quercetin water solubility with steviol glucosides and the studies of biological properties

2015 ◽  
Vol 5 (12) ◽  
pp. 437 ◽  
Author(s):  
Thi Thanh Hanh Nguyen ◽  
Shin-Hye Yu ◽  
Jiyoun Kim ◽  
Eunbae An ◽  
Kyeonghwan Hwang ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Mushroom Tyrosinase ◽  
Human Beings ◽  
Inhibition Activity ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Human Intestinal Maltase ◽  
Dpph Radical Scavenging

Background: Quercetin, a flavonol contained in various vegetables and fruits, has various biological activities including anticancer, antiviral, anti-diabetic, and anti-oxidative. However, it has low oral bioavailability due to insolubility in water. Thus, the bioavailability of quercetin administered to human beings in a capsule form, was reported to be less than 1%, with only a small percentage of ingested quercetin getting absorbed in the blood. This leads to certain difficulties in creating highly effective medicinesMethods: Quercetin-rubusoside and quercetin-rebaudioside were prepared. The antioxidant activities of quercetin and Q-rubusoside were evaluated by DPPH radical scavenging method. Inhibition activities of quercetin and Quercetin-rubusoside were determined by measuring the remaining activity of 3CLpro with 200 μM inhibitor. The inhibition activity of quercetin, rubusoside and quercetin-rubusoside were determined by measuring the activity of human maltase which remains at 100 μM rubusoside or quercetin-rubusoside. The mushroom tyrosinase inhibition was assayed with the reaction mixture contained 3.3 mM L-DOPA in 50 mM potassium phosphate buffer (pH 6.8), and 10 U mushroom tyrosinase/ml with or without quercetin or quercetin-rubusoside. Results: With 10% rubusoside treatment, quercetin showed solubility of 7.7 mg/ml in water, and its solubility increased as the concentration of rubusoside increased; the quercetin solubility in water increased to 0.83 mg/mlas rubusoside concentration increased to 1 mg/ml. Quercetin solubilized in rubusoside solution showed DPPH radical-scavenging activity and mushroom tyrosinase inhibition activity, similar to that of quercetin solubilized in dimethyl-sulfoxide. Quercetin-rubusoside also showed 1.2 and 1.9 folds higher inhibition activity against 3CLpro of SARS and human intestinal maltase, respectively, than those of quercetin in DMSO.Conclusions: Quercetin can be solubilized in water with rebaudioside or rubusoside treatment. As Ru concentration increases, the solubility of quercetin in water increases. The solubilization of quercetin in Ru solution did not reduce its biological functions such as the DPPH radical-scavenging and mushroom tyrosinase activity; also, quercetin-rubusoside increased the inhibition activity against the 3CLpro of SARS and human intestinal maltase, when compared with the activity of quercetin in DMSO. Thus, rubusoside and rebaudioside are promising compounds which enhance the solubility of poorly water soluble compounds.Keywords: rubusoside, rebaudioside, flavonol, quercetin, human maltase, 3CLpro

scholarly journals Antioxidant and antimicrobial activities of different enzymatic hydrolysates from desalted duck egg white

2020 ◽  
Vol 33 (9) ◽  
pp. 1487-1496 ◽  
Author(s):  
Rommanee Thamamsena ◽  
Deng Cheng Liu
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Egg White ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Hydrolysis Time ◽  
Dpph Radical Scavenging ◽  
Antioxidant And Antimicrobial Activities

Objective: The objective of this study was to look for optimal preparation of hydrolysates of desalted duck egg white powder (DDEWP) by the three different proteases and to investigate their antioxidant and antimicrobial properties.Methods: DDEWP was hydrolyzed by three proteases, including pepsin (PEP), Bacillus spp. (BA) and natokinase (NAT) with three different enzyme concentrations (0.1%, 0.3%, and 0.5%), individually. The important key hydrolysis parameters such as hydrolysis degree, yield, antioxidant and antimicrobial activity were evaluated in this experiment.Results: The results showed that the degree of hydrolysis (DH) of all treatments increased with increasing hydrolysis time and protease concentrations. The antioxidant and antimicrobial activities of the hydrolysates were affected by type and concentration of protease as well as hydrolysis time. Hydrolysis of PEP significantly (p<0.05) obtained the highest yield of hydrolysates, however, both of BA and NAT showed substantially lower DH values and still did not exceed 5% by the end of hydrolysis. Among the different hydrolysates, PEP exhibited significantly higher 2, 2–diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than BA and NAT. All DDEWP hydrolysates from PEP had low ferrous ion chelating activity (<37%) that was significantly lower than that of NAT (>37% to 92%) and BA (30% to 79%). Besides, DDEWP hydrolysates of PEP presented significantly higher reducing power than BA and NAT. In antimicrobial activities, Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa were not effectively inhibited by any DDEWP hydrolysates of PEP except for Staphylococcus aureus. Especially, the excellent antibacterial activity against S. aureus only was displayed in DDEWP hydrolysates of PEP 0.1%.Conclusion: DDEWP hydrolysates from PEP demonstrated significantly better DH, yield, DPPH radical scavenging activity and reducing power, furthermore, had excellent inhibitory on S. aureus due to large clear zone and moderated inhibitory in bactericidal inhibition.

scholarly journals An Investigation on Biological Efficacies of Fruits and Leaves of Ficus auriculata (Timilo) from Kanchanpur District, Nepal

2021 ◽  
Vol 2 (01) ◽  
pp. 51-58
Author(s):  
Bishan Datt Bhatt ◽  
Dharma Raj Joshi
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Dpph Radical ◽  
Leaf Extracts ◽  
E Coli ◽  
Methanol Extracts ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Ficus auriculata is a native Asian plant found in the temperate, tropical and subtropical regions and has been commonly used in traditional medicine and as fodder in animal husbandry. The comparative antibacterial and antioxidant efficacies of leaves and fruits have been studied using their hexane, chloroform, ethyl acetate and methanol extracts. Phytochemical screening exhibited the presence of important secondary metabolites like alkaloids, carbohydrates, glycosides, flavonoids and tannins. Antibacterial activities of fruit and leaf extracts in different concentrations were studied against E. coli, S. aureus and S. typhi by agar well diffusion method. The highest inhibition was found to be in 1% methanol extracts of leaves and fruits with a zone of inhibition (ZOI) ± 16 mm against S. aureus followed by E. coli and S. typhi with ZOI ±14 mm. The crude and 50% extracts of various solvents of both fruits and leaves were found to be ineffective against bacteria. These results reveal that there is a significant antibacterial activity in methanol extract of both fruit and leaves, against gram-positive and gram-negative bacteria. The antioxidant activities of methanol extracts of fruits and leaves were studied by DPPH radical scavenging assay. The IC50 values of methanol extract of leaves and fruit for DPPH radical scavenging assay were found to be 114.84 μg/mL and 78.28 μg/mL, respectively. These results reveal that methanol fruit extract exhibits better antioxidant activity as compared to the leaves. The result of this investigation has revealed the applicability of this plant as a potential source of several bioactive compounds for the discovery of new and efficacious drugs in days to come.

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