scholarly journals Antioxidant and cytotoxic activities of Artemisia monosperma L. and Tamarix aphylla L. essential oils

2021 ◽  
Vol 49 (1) ◽  
pp. 12233
Author(s):  
Ramy M. ROMEILAH ◽  
Hossam S. EL-BELTAGI ◽  
Emad A. SHALABY ◽  
Kareem M. YOUNES ◽  
Hani EL MOLL ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Principal Component ◽  
Cytotoxic Agents ◽  
Promyelocytic Leukaemia ◽  
Dpph Radical ◽  
Leukaemia Cell ◽  
Dpph Radical Scavenging

Essential (volatile) oil from leaves of Artemisia monosperma L. belonging to family Asteraceae, and aerial parts of Tamarix aphylla L. (Athel) belonging to family Tamaricaceae were collected from the desert of Ha'il region, northern region of Saudi Arabia, hydro distilled by Clevenger apparatus and analysed by means of GC-MS techniques. Antioxidant activities of essential oils of A. monosperma and T. aphylla compared with ascorbic acid and butylated hydroxytoluene (BHT) as reference antioxidant compound were determined by method of DPPH radical scavenging assay and ABTS assay. In vitro screening of potential cytotoxicity of essential oils was also evaluated against human promyelocytic leukaemia cell lines (HL60 and NB4). The GC/MS analysis of A. monosperma essential oil resulted in identification of 61 components predominated mainly by β-Pinene as principal component (29.87%) and T. aphylla resulted in identification of 37 components of essential oil predominated mainly by 6,10,14- trimethyl-2-pentadecanone (21.43%) as principal component. Antioxidant activity as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) increased with increasing essential oil concentrations of A. monosperma and T. aphylla (25, 50, 75, 100 and 200 μg mL-1). The most pronounced increases detected in the high concentrations of the two essential oils. Biologically, essential oil extracts exhibited cytotoxicity effects in dose dependent manner against human promyelocytic leukaemia cell lines (HL60 and NB4). In conclusion, A. monosperma and T. aphylla essential oils could be valuable source for cytotoxic agents with high safety and selective cytotoxicity profiles.

scholarly journals Antioxidant and Anti-inflammatory Activities of Essential Oils of Actinodaphne macrophylla and A. pruinosa (Lauraceae)

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Farediah Ahmad
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Significant Activity ◽  
Dpph Radical ◽  
Leaf Oil ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

The essential oils of Actinodaphne macrophylla and A. pruinosa were analyzed by GC and GC-MS and evaluated for their antioxidant and anti-inflammatory activities. The essential oil of the leaves of A. macrophylla was characterized by the presence of 31 components (78.2%), with germacrene B (16.8%) and globulol (16.0%) as the major components. A total of 28 components (71.6%) were characterized in the leaf oil of A. pruinosa with the most abundant components being globulol (17.8%) and spathulenol (12.0%). The antioxidant activity was determined by DPPH radical scavenging and total phenolic content, while anti-inflammatory activity by lipoxygenase assay. The essential oil of A. pruinosa demonstrated significant activity on DPPH (IC50 85.6 μg/mL), phenolic content (190.2 mg GA/g) and in the lipoxygenase (IC5085.2 μM) assays.

Carum copticum L. essential oils as natural antimicrobial and antioxidant

2018 ◽  
Vol 44 (4) ◽  
pp. 515-520
Author(s):  
M Kazemi
Keyword(s):  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Carum Copticum ◽  
Microbial Strains ◽  
Pharmaceutical Industries ◽  
Dpph Radical Scavenging ◽  
Antioxidant Effects

Carum copticum L. comprises several relevant species for food, cosmetic, perfumery and pharmaceutical industries. GC/MS analysis of the enential oil of C. copticum revealed γ- terpinene as a major component of C. copticum, with its contribution of 33.85%. Essential oils (EOs) exhibited a significant antimicrobial activity against all tested microbial strains. In addition, the C. copticum oil demonstrated the highest DPPH radical scavenging activity. These results clearly show the antimicrobial and antioxidant effects of the plant essential oil.

scholarly journals Antibacterial Activities of Essential Oils Extracted from Leaves of Murraya koenigii by Solvent-Free Microwave Extraction and Hydro-Distillation

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Naciye Erkan ◽  
Zhou Tao ◽  
H. P. Vasantha Rupasinghe ◽  
Burcu Uysal ◽  
Birsen S. Oksal
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Solvent Free ◽  
Microwave Extraction ◽  
Murraya Koenigii ◽  
Inhibition Of Growth ◽  
Germacrene D ◽  
Dpph Radical Scavenging

Solvent-free microwave extraction (SFME) for the isolation of essential oil from leaves of Murraya koenigii L. (Rutaceae) has been compared with the conventional hydro-distilled oil (HD) in terms of yield, composition, antioxidant activity, and antibacterial activity against Listeria innocua. The yield of essential oil obtained from 30 min of SFME was similar to that of HD for 180 min. By GC-MS analysis, the major compounds of the essential oil extracted by SFME, which were obtained in somewhat lower amounts than in the essential oil obtained by HD, were α-copaene (44.3%), β-gurjunene (25.5%), isocaryophyllene (12.1%), β-caryophyllene (8.7%) and germacrene D (2.9%). The content of oxygenated terpenes, slightly higher for the SFME-essential oil (2.3%) than the HD-essential oil (1.4%), were much lower than that of nonoxygenated terpenes in both oils. DPPH radical scavenging activities of both essential oils were relatively low (10%-24%). Complete inhibition of growth of L. innocua was observed with both SFME and HD essential oils, at 400 and 600 μg/mL (minimum inhibitory concentration), respectively. The SFME-essential oil at 300 μg/mL provided 92% inhibition, indicating its potential as a natural antimicrobial agent.

scholarly journals Development of Multifunctional Cosmetic Cream Using Bioactive Materials from Streptomyces sp. T65 with Synthesized Mesoporous Silica Particles SBA-15

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 278
Author(s):  
Ram Hari Dahal ◽  
Tuan Manh Nguyen ◽  
Dong Seop Shim ◽  
Joon Young Kim ◽  
Jangyul Lee ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Type I ◽  
Mushroom Tyrosinase ◽  
Dpph Radical ◽  
Crude Product ◽  
Dpph Radical Scavenging

Various cosmetics having a single function are increasingly being used, but cosmetics having multifunctional activities remain limited. We aimed to develop a multifunctional cosmetic cream having antioxidant, anti-tyrosinase, anti-aging and antimicrobial activities. Antimicrobial activities were performed by disc-diffusion method. Cell toxicity and cell proliferations were evaluated in a 96-well plate with different cell lines such as HaCaT, RAW264.7, CCD-986Sk, B16F1, and B16F10. Mushroom tyrosinase inhibition, elastase inhibition, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were evaluated and IC50 was calculated. Mesoporous silica particle was synthesized using Pluronic P123 and tetraethyl ortho-silicate (TEOS). Facial pictures were captured by VISIA-CR (Facial Imaging System for Clinical Research). Roughness of image was analysed by PRIMOS software and brightness of image was analyzed by Chromameter CR-400. The crude product of strain T65 inhibited the different human pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Propionibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The IC50 of T65 crude product for mushroom tyrosinase, elastase, and DPPH radical scavenging activities were 58.73, 14.68, and 6.31 µg/mL, respectively. T65 crude product proliferated collagen type I in CCD-986Sk cell up to 145.91% ± 9.11% (mean ± SD; mean of 24, 48, and 72 h) at 250 pg/mL. Synthesized mesoporous particles (SBA-15) confirmed the sustainable performance by control-release for three days. Formulated functional cosmetic cream containing T65 embedded SBA-15, significantly decreased the skin roughness by 4.670% and increased the skin brightness by 0.472% after application of 4 weeks. T65 crude product inhibited both Gram-positive and Gram-negative pathogens. Synthesized mesoporous particle, SBA-15, confirmed the physiologically active substance was released in sustainable release condition. T65 crude product showed impeccable antimicrobial, antioxidant, anti-aging, and whitening activities with non-cytotoxic effects to different cell lines related to the human skin.

scholarly journals Comparative Chemical Composition and Antioxidant Properties of the Essential Oils of three Sideritis libanotica Subspecies

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Carmen Formisano ◽  
Filomena Oliviero ◽  
Daniela Rigano ◽  
Nelly Apostolides Arnold ◽  
Felice Senatore
Keyword(s):  
Essential Oils ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Ferric Ion ◽  
Main Constituent ◽  
Dpph Radical ◽  
Hexadecanoic Acid ◽  
Phytochemical Composition ◽  
Dpph Radical Scavenging

The phytochemical composition of the essential oils of three Sideritis libanotica subspecies, namely S. libanotica ssp. libanotica, S. libanotica ssp. linearis and S. libanotica ssp. michroclamys, all collected in Lebanon, was analyzed by GC and GC-M S. The diterpene sideridiol was recognized as the main constituent of both S. libanotica ssp. libanotica (50.8%) and S. libanotica ssp. michroclamys (18.4%) oils, while hexadecanoic acid (10.5%) prevailed in S. libanotica ssp. linearis. The antioxidant activity of the oils was studied in two cell free systems by DPPḢ radical scavenging and ferric ion reduction (FRAP) assays; only S. libanotica ssp. linearis showed a moderate activity when assayed by the FRAP test (0.6± 0.01 mmol TE/mL).

scholarly journals Radical Scavenging and Antioxidant Activities of Essential Oil Components – An Experimental and Computational Investigation

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Farukh S. Sharopov ◽  
Michael Wink ◽  
William N. Setzer
Keyword(s):  
Essential Oil ◽  
Electronic Properties ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Oil Components ◽  
Dpph Radical Scavenging

The antioxidant activities of eighteen different essential oil components have been determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, the 2,2 ’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation assay, and the ferric reducing antioxidant power (FRAP) assay. The phenolic compounds, carvacrol, thymol, and eugenol, showed the best antioxidant activities, while camphor, menthol, and menthone were the least active. The structural and electronic properties of the essential oil components were assessed using density functional theory (DFT) at the B3LYP/6-311++G** level. Correlations between calculated electronic properties and antioxidant activities were generally poor, but bond-dissociation energies (BDEs) seem to correlate with DPPH radical-scavenging activities, and the ferric reducing antioxidant power (FRAP) assay correlated with vertical ionization potentials calculated at the Hartree-Fock/6-311++G** level.

scholarly journals Antibacterial, Antiviral, Antioxidant and Antiproliferative Activities of Thymus guyonii Essential Oil

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Assia Zeghib ◽  
Ahmed Kabouche ◽  
Souheila Laggoune ◽  
Claude-Alain Calliste ◽  
Alain Simon ◽  
...  
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Bactericidal Effect ◽  
Dpph Radical ◽  
Aerial Parts ◽  
Colorectal Cell ◽  
Test Concentration ◽  
Dpph Radical Scavenging ◽  
Ht 29

The aerial parts of Thymus guyonii were hydrodistilled in a Clevenger type apparatus and analyzed by GC and GC-MS. 26 components were identified representing 99.8%, with carvacrol (55.6%), thymol (21.2%), o-cymene (9.7%) and δ-terpinene (5.7%) as the major components. T. guyonii essential oil exhibited a good bactericidal effect (MBC/MIC ratio = 2) against all the tested pathogenic bacteria. The essential oil lacks antiviral activity towards coxsackievirus B3 however it showed a good DPPH radical scavenging at 0.5 and 5 mg/mL test-concentrations (15.5±1.2% and 44.3±1.95%, respectively). The essential oil exhibited a proliferation inhibition of Human colorectal cell line HT-29 (30.4±3.4%) at 50 μg/mL test-concentration.

scholarly journals Determination of phenolic compounds and antioxidant activity of green, black and white teas of Camellia sinensis (L.) Kuntze, Theaceae

2014 ◽  
Vol 16 (3) ◽  
pp. 490-498 ◽  
Author(s):  
V.P Pereira ◽  
F.J Knor ◽  
J.C.R Vellosa ◽  
F.L Beltrame
Keyword(s):  
Camellia Sinensis ◽  
Plant Material ◽  
Radical Scavenging ◽  
Principal Component ◽  
Total Phenols ◽  
Considerable Variability ◽  
Dpph Radical ◽  
Black And White ◽  
Dpph Radical Scavenging

Green, black and white teas are all produced from leaves and shoots of Camellia sinensis, the only difference is how they are processed. The aim of this study was to compare the total phenols and flavonoid contents and antioxidant capacity of green, black and white tea bags of different brands. The morphodiagnosis of leaves was used to identification of plant material. HPLC-DAD fingerprinting coupled with Principal Component Analysis (PCA) was applied to analyze similarities of the tea samples. The results showed considerable variability between tea brands in both total phenols (30.55 to 60.85 mg of pyrogallol/g) and flavonoids (6.35 to 8.92 mg of quercetin/g). Green and white teas demonstrated the highest ABTS and DPPH radical scavenging activities.

Chemical Composition and TLC-DPPH-Radical Scavenging Activity ofCyperus alternifoliusRottb. Essential Oils

2017 ◽  
Vol 20 (4) ◽  
pp. 1125-1130 ◽  
Author(s):  
Shimaa S. Elshrif ◽  
Abd El-Nasser G. El Gendy ◽  
Abdelsamed I. Elshamy ◽  
Mahmoud I. Nassar ◽  
Hesham R. El-Seedi
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

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