scholarly journals Screening of Antibacterial Activity, Antioxidant Activity, and Anticancer Activity of Euphorbia hirta Linn. Extracts

2020 ◽  
Vol 10 (23) ◽  
pp. 8408
Author(s):  
Ngan Tran ◽  
Minh Nguyen ◽  
Khanh PB Le ◽  
Nhi Nguyen ◽  
Quan Tran ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Antimicrobial Activity ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Biological Activities ◽  
Lung Cancer Cells ◽  
Diffusion Method ◽  
Hep G2 ◽  
Euphorbia Hirta ◽  
Liver Cancer Cells

This study aimed to screen the anticancer and antioxidant potential and antimicrobial activity of methanol, petroleum ether, chloroform, ethyl acetate, butanol of Euphorbia hirta Linn. extracts (EH-Me, EH-PE, EH-Ch, EH-EA and EH-Bu, respectively). The results of 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging assay and lipid peroxidation inhibition assay showed that EH-EA was the strongest antioxidant (IC50 = 10.33 ± 0.01 µg/mL; IC50 = 1.48 ± 0.12 µg/mL, respectively) compared to all other extracts. In the antimicrobial activity of the extracts against eight strains of Gram-positive and Gram-negative bacteria using the agar disc diffusion method, we found the EH-EA to be the best antimicrobial agent. Anticancer activities of those extracts were examined by sulforhodamine B (SRB) in vitro cytotoxicity assay on two cancer cell lines, including lung cancer cells NCI-H460 and liver cancer cells Hep G2. EH-EA at concentration of 100 μg/mL has significant inhibitory activity the growth of lung cancer cells NCI-H460 and liver cancer cells Hep G2 compared to all other extracts. Our results suggest that E. hirta Linn. extracts possess significant biological activities, including antimicrobial, antioxidant, and moderate anticancer properties. Our results show that this plant could be a good source for natural antioxidants and a possible pharmaceutical supplement. Among five analyzed extracts, EH-EA extract has the strongest activities, and should be used to determine phytochemicals and mechanisms of these activities.

scholarly journals Synthesis of new dihydroberberine and tetrahydroberberine analogues and evaluation of their antiproliferative activity on NCI-H1975 cells

2020 ◽  
Vol 16 ◽  
pp. 1606-1616
Author(s):  
Giacomo Mari ◽  
Lucia De Crescentini ◽  
Serena Benedetti ◽  
Francesco Palma ◽  
Stefania Santeusanio ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Biological Properties ◽  
The Other ◽  
Lung Cancer Cells ◽  
Reduced Form ◽  
Other Hand

Dihydroberberine (DHBER), the partially reduced form of the alkaloid berberine (BER), is known to exhibit important biological activities. Despite this fact, there have been only few studies that concern the biological properties of functionalized DHBER. Attracted by the potentiality of this latter compound, we have realized the preparation of new arylhydrazono-functionalized DHBERs, starting from BER and some α-bromohydrazones. On the other hand, also the fully reduced form of BER, namely tetrahydroberberine (THBER), and its derivatives have proven to present different biological activities. Therefore, the obtained arylhydrazono-functionalized DHBERs were reduced to the corresponding arylhydrazono-THBERs. The antiproliferative activity of both arylhydrazono-DHBERs and -THBERs has been evaluated on NCI-H1975 lung cancer cells.

Syntheses, structural characterization and biological activities of spiro-ansa-spiro-cyclotriphosphazenes

2015 ◽  
Vol 39 (11) ◽  
pp. 8825-8839 ◽  
Author(s):  
Nisan Sevin Başterzi ◽  
Selen Bilge Koçak ◽  
Aytuğ Okumuş ◽  
Zeynel Kılıç ◽  
Tuncer Hökelek ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Binding ◽  
Cancer Cells ◽  
Structural Characterization ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Lung Cancer Cells

spiro-ansa-spiro-Phosphazenes were investigated for their DNA binding, antimicrobial activities and cytotoxic, apoptotic, and necrotic effects on fibroblast and lung cancer cells.

Allicin Induces p53-Mediated Autophagy in Hep G2 Human Liver Cancer Cells

2012 ◽  
Vol 60 (34) ◽  
pp. 8363-8371 ◽  
Author(s):  
Yung-Lin Chu ◽  
Chi-Tang Ho ◽  
Jing-Gung Chung ◽  
Raghu Rajasekaran ◽  
Lee-Yan Sheen
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Hep G2 ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

scholarly journals Effects of siRNA targeting BMPR-II on the biological activities of human liver cancer cells and its mechanism

2014 ◽  
Vol 14 (1) ◽  
pp. 55 ◽  
Author(s):  
Peng Zeng ◽  
Sheng Cai ◽  
Jia-na Zhang ◽  
Feng-ming Yi ◽  
Wei-min Jiang ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Biological Activities ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

scholarly journals Chemoprevention of NCI-H322 Lung Cancer Cells Proliferation and Tumor Regression in Murine Ascites Model by Dietary Flavonoid Quercetin

2021 ◽  
Vol 12 (5) ◽  
pp. 6950-6959
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tumor Regression ◽  
Biological Activities ◽  
Cancer Cell Lines ◽  
Prunus Cerasus ◽  
Lung Cancer Cells

Prunus cerasus L (Sour cherries) contain diverse secondary metabolites which exhibit various biological activities, including anticancer, antioxidant, and anti-inflammatory properties. The present study aimed to determine the anticancer efficacy of four compounds, quercetin, daidzin, rutin, and chlorogenic acid, isolated from Prunus cerasus fruit. The antiproliferative activity of four cherry isolates was determined against five different cancer cell lines (NCI-H322, A549, THP-1, MCF-7, and PC-3) by Tetrazolium bromide assay, followed by apoptosis Cell cycle analyses, mitochondrial membrane potential, cell migration test, and in vivo Ehrlich Ascites Carcinoma studies using potent bioactive lead. The cytotoxicity profile of the four molecules demonstrated that quercetin induced significant cell growth inhibition in all cancer cell lines with paramount 79% cytotoxicity against NCI-H322 lung cancer cells (IC50 value 24μM). Incubation of NCI-H322 cells with quercetin showed a concentration-dependent increase in hypo-diploid sub G0/G1 DNA fraction, exhibited consequential changes in nuclear morphology, and caused mitochondrial transmembrane potential loss of 60.3% augmented at 30 µM. Pertaining to in vivo potency, quercetin manifested 89% tumor inhibition at 50 mg/kg body weight in EAC-bearing mice. The current studies raise the potential usefulness of quercetin in chemoprevention against lung cancer cells and support its empirical use as a promising nutraceutical agent.

scholarly journals Anticancer Potential of Synthesized Silver Nanoparticles Using Bioactive Metabolites of Actinobacteria Against A549 Lung Cancer Cells

2021 ◽  
Author(s):  
Naushin Bano ◽  
Saba Siddiqui ◽  
Adria Hasan ◽  
Mohammad Amir ◽  
Snober S Mir ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Secondary Metabolites ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Vis Spectroscopy ◽  
A549 Lung

Abstract Multi-drug resistance microorganisms and the rising numbers of cancer cases possess a critical threat to humankind, thereby motivating research on new weapons to combat the problem. To address these issues, researchers are now focusing on secondary metabolites produced by bacteria. Because of having outstanding antibiotic capabilities, actinobacteria are being explored as a potential solution to this problem. Silver nanoparticles of actinobacteria are green, eco-friendly, and cost-effective, as well as having antibacterial and anti-cancer properties and potential use in pharmaceuticals. Antibacterial potential of secondary metabolites produced by actinobacteria namely Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O) has been demonstrated by the well diffusion method. GC-MS was used to detect compounds in bioactive metabolites. The most abundant compound found in metabolites was n-hexadecanoic acid. UV-Vis spectroscopy was used to determine the extracellular development of silver nanoparticles of actinobacteria secondary metabolites, while transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) were used to inspect their morphological appearances, stability, crystalline structure, and coating. The activity of anticancer was assessed using the MTT test to evaluate the cytotoxicity of the secondar metabolites (pure and nanoparticles) against A549 lung cancer cells. We also evaluated the effect of secondar metabolites (pure and nanoparticles) on Reactive oxygen species (ROS) levels, Mitochondrial membrane potential (MMP) and Chromatin condensation through DCFDA, Mito Tracker and DAPI staining. Our result suggested that their secondary metabolites can be used a potential lead compound against cancer. However, more investigation is needed to totally grasp their mechanism of action.

Nanoparticles of two ZnO Precursors as an Encapsulating Matrix of Mangiferin: Associated Studies to Cytotoxic Effects on Liver Cancer Cells Hep-G2 and Healthy Lung Cell Beas-2B

2021 ◽  
Author(s):  
Francisco Fabián Razura-Carmona ◽  
Mayra Herrera-Martínez ◽  
Sonia G. Sáyago-Ayerdi ◽  
Alejandro Pérez-Larios ◽  
Efigenia Montalvo-González ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Hep G2 ◽  
Cytotoxic Effects ◽  
Liver Cancer Cells ◽  
Healthy Lung

Tu2033 Role of Methionine Adenosyltransferase 2β in Resveratrol's Biological Activities in Liver Cancer Cells

2013 ◽  
Vol 144 (5) ◽  
pp. S-909
Author(s):  
Heping Yang ◽  
Yuhua Zheng ◽  
Adriana L. Rojas ◽  
Jose M. Mato ◽  
Shelly C. Lu
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Biological Activities ◽  
Methionine Adenosyltransferase ◽  
Liver Cancer Cells

scholarly journals Anti-Proliferative Activity of Triterpenes Isolated from Clinicanthus nutans on Hep-G2 Liver Cancer Cells

2019 ◽  
Vol 20 (2) ◽  
pp. 563-567 ◽  
Author(s):  
Khairun Najwa Zakaria ◽  
Azura Amid ◽  
Zubaidah Zakaria ◽  
Parveen Jamal ◽  
Azli Ismail
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Proliferative Activity ◽  
Hep G2 ◽  
Liver Cancer Cells

scholarly journals Mussaenin A Isolated From Mussaenda glabrata Induces Apoptosis in the Liver Cancer Cells Via Mitochondrial Pathway

2017 ◽  
Vol 9 (09) ◽  
Author(s):  
Lipin Dev Mundur Sahadevan ◽  
Darsan Balakrishnan Menon
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Mitochondrial Pathway ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Caspase 9 ◽  
Nih3t3 Cells ◽  
Liver Cancer Cells ◽  
G2 Cells ◽  
Apoptotic Factors

Plants belonging to Rubiaceae family have been used in Chinese folk medicine and Ayurveda. The aim of the study was to determine the effect of Mussaenin A isolated from root of Mussaenda glabrata on liver cancer cell line, Hep G2. MTT assay was performed to check the ability of Mussaenin A to induce death in cancerous Hep G2 cells and normal NIH3T3 cells. Acridine Orange/Ethidium Bromide staining, Hoechst staining and DNA fragmentation assays were used to confirm the apoptosis inducing ability of MA on Hep G2 cells. RT-PCR and western blotting was performed to check the expression of pro- and anti-apoptotic factors. Calorimetric assay was done to check caspase-3 and caspase-9 activities. Mussaenin A at lower concentrations was found to induce cell death selectively in the liver cancer cells (IC50 = 11.38 µg/mL). The transcriptional expression studies of the pro-apoptotic Bax and anti-apoptotic Bcl-2 and Cox-2; and the western blot analysis of pro-apoptotic BAK and BAD showed that MA upregulated the expression of pro-apoptotic factors and down regulated the expression of anti-apoptotic factors in Hep G2 cells. Caspase-9 and 3 activities were found to be upregulated in the calorimetric studies. The down regulation of anti-apoptotic factors and upregulation of pro-apoptotic factors show that the Mussaenin A induced apoptosis in the liver cancer cells via the intrinsic or the mitochondrial pathway.

Sign in / Sign up

Export Citation Format

Share Document