scholarly journals A Comparative Cross-Platform Meta-Analysis to Identify Potential Biomarker Genes Common to Endometriosis and Recurrent Pregnancy Loss

2021 ◽  
Vol 11 (8) ◽  
pp. 3349
Author(s):  
Pokhraj Guha ◽  
Shubhadeep Roychoudhury ◽  
Sobita Singha ◽  
Jogen C. Kalita ◽  
Adriana Kolesarova ◽  
...  
Keyword(s):  
Pregnancy Loss ◽  
Reproductive Tract ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Female Reproductive Tract ◽  
Reproductive Age ◽  
Functional Enrichment ◽  
Common Source ◽  
Human Populations ◽  
Potential Biomarker

Endometriosis is characterized by unwanted growth of endometrial tissue in different locations of the female reproductive tract. It may lead to recurrent pregnancy loss, which is one of the worst curses for the reproductive age group of human populations around the world. Thus, there is an urgent need for unveiling any common source of origin of both these diseases and connections, if any. Herein, we aimed to identify common potential biomarker genes of these two diseases via in silico approach using meta-analysis of microarray data. Datasets were selected for the study based on certain exclusion criteria. Those datasets were subjected to comparative meta-analyses for the identification of differentially expressed genes (DEGs), that are common to both diagnoses. The DEGs were then subjected to protein-protein networking and subsequent functional enrichment analyses for unveiling their role/function in connecting two diseases. From the analyses, 120 DEGs are reported to be significant out of which four genes have been found to be prominent. These include the CTNNB1, HNRNPAB, SNRPF and TWIST2 genes. The significantly enriched pathways based on the above-mentioned genes are mainly centered on signaling and developmental events. These findings could significantly elucidate the underlying molecular events in endometriosis-based recurrent miscarriages.

scholarly journals The association between female reproductive tract microbiota and recurrent pregnancy loss: a nested case-control study

2021 ◽  
Author(s):  
Pirkko Peuranpää
Keyword(s):  
Pregnancy Loss ◽  
Reproductive Tract ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Amplicon Sequencing ◽  
Case Control ◽  
Vaginal Microbiota ◽  
Female Reproductive Tract ◽  
Nested Case Control ◽  
Control Study

Abstract Objective To study the associations of endometrial and vaginal microbiota with recurrent pregnancy loss (RPL). Design A nested case-control study. Setting Helsinki University Hospital, Finland. Population Women with two or more consecutive pregnancy losses (n=47) and healthy control women without a history of pregnancy loss (n=39). Methods The endometrial and vaginal microbiota compositions, analysed using 16S rRNA gene amplicon sequencing, were compared between RPL women and controls, and between individual vaginal and endometrial samples. The mycobiota composition was analysed using internal transcribed spacer 1 amplicon sequencing for a descriptive summary. The models were adjusted for confounding variables BMI, age, and parity, and FDR-corrected P-values (q-values) were used to define nominal statistical significance at q < 0.05. Main outcome measures Mean relative microbial abundances. Results Lactobacillus crispatus was less abundant in the RPL endometrial samples compared to controls (mean relative abundance 17.2% vs. 45.6%, q = 0.04). Gardnerella vaginalis was more abundant in RPL women than in controls in both endometrial (12.4% vs. 5.8%, q < 0.001) and vaginal samples (8.7% vs. 5.7%, q < 0.01). The individual vaginal and endometrial microbial composition correlated strongly (R = 0.85, P < 0.001). Fungi were detected in 22% of the endometrial and 36% of the vaginal samples. Conclusions Unfavourable reproductive tract microbiota was associated with RPL and may represent a novel risk factor for pregnancy losses.

scholarly journals Effectiveness of integrated Chinese herbal medicine Shoutai Pill and Western medicine in the treatment of recurrent pregnancy loss: A protocol for systematic review and meta-analysis

2021 ◽  
Author(s):  
Mu Zhenni ◽  
Le Lei ◽  
Shen Sinan ◽  
Tang Li
Keyword(s):  
Psychological Health ◽  
Western Medicine ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Genetic Diagnosis ◽  
Reproductive Age ◽  
Endocrine Disorders ◽  
Review Question ◽  
Physical And Psychological Health

Review question / Objective: We provide a protocol to evaluate the efficacy of integrated Shoutai Pill and Western medicine to update the evaluation for the best available and security treatment for recurrent pregnancy loss(RPL). Condition being studied: Recurrent pregnancy loss (RPL) is a distinct disorder defined by two or more consecutive pregnancy failures before 20 gestational weeks infertile couples. The incidence of this disease accounts for about 1%-5% of women of reproductive age and seriously affects their physical and psychological health. At present, the known etiology of this disease mainly includes abnormal anatomic structures, genetic abnormality, endocrine disorders, prethrombotic status, abnormal immune function, infection, etc. Excluding the above factors, approximately 40-50% of RPL remain unexplained, known as unexplained recurrent pregnancy loss (URPL). At present, the main therapeutic methods of RPL are surgical therapy, preimplantation genetic diagnosis (PGD), hormone therapy, anti-infection therapy, anticoagulation, and immunoregulatory therapy, etc. However, there is no effective treatment has been identified for URPL. Therefore, we still need to investigate effective treatments to reduce pregnancy losses and maintain successful pregnancy preservation in these patients.

scholarly journals Systematic review and meta-analysis of female lifestyle factors and risk of recurrent pregnancy loss

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ka Ying Bonnie Ng ◽  
George Cherian ◽  
Alexandra J. Kermack ◽  
Sarah Bailey ◽  
Nick Macklon ◽  
...  
Keyword(s):  
Systematic Review ◽  
General Population ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Lifestyle Factors ◽  
Caffeine Intake ◽  
Increased Risk ◽  
Secondary Outcomes

AbstractIt is known that lifestyle factors affect sporadic miscarriage, but the extent of this on RPL (recurrent pregnancy loss) is less well known. A systematic review and meta-analysis was performed to assess the associations between lifestyle factors and RPL. Studies that analysed RPL in the context of BMI, smoking, alcohol and caffeine intake were included. The primary and secondary outcomes were odds of having RPL in the general population and odds of further miscarriage, respectively. Underweight and women with BMI > 25 are at higher odds of RPL in the general population (OR 1.2, 95% CI 1.12–1.28 and OR 1.21, 95% CI 1.06–1.38, respectively). In women with RPL, having BMI > 30 and BMI > 25 has increased odds of further miscarriages (OR 1.77, 95% CI 1.25–2.50 and OR 1.35, 95% CI 1.07–1.72, respectively). The quality of the evidence for our findings was low or very low. Being underweight and BMI > 25 contributes significantly to increased risk of RPL (general population). BMI > 25 or BMI > 30 increases the risk of further miscarriages (RPL population). Larger studies addressing the effects of alcohol, cigarette smoking and caffeine on the risk of RPL with optimisation of BMI in this cohort of women are now needed.

Association of VEGF and p53 Polymorphisms and Spiral Artery Remodelling in Recurrent Pregnancy Loss: A Systematic Review and Meta-analysis

2021 ◽  
Author(s):  
Tamilmani Subi ◽  
Vinodhini Krishnakumar ◽  
Chandreswara Raju Kataru ◽  
Inusha Panigrahi ◽  
Meganathan Kannan
Keyword(s):  
Risk Factor ◽  
Risk Ratio ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Mixed Population ◽  
Relative Risk Ratio ◽  
Spiral Artery ◽  
Prevalence Odds Ratio ◽  
Increased Risk

Many studies have reported the association of VEGF-1154G/A, VEGF 936C/T and p53 Arg72Pro polymorphisms with Recurrent Pregnancy Loss (RPL), but the outcomes are inconsistent. We have used meta-analysis to associate these polymorphisms with RPL, having the spiral artery remodelling as a major risk factor. The studies were identified from three different reputed databases, namely Science direct, PubMed/Medline and Scopus. The eligible studies of VEGF-1154G/A, VEGF 936C/T and p53Arg72Pro polymorphisms associated with the RPL were selected for the analysis. They were segregated into three different ethnic groups as Asians, Caucasians and mixed population. For the analysis, the overall prevalence, Odds ratio, Risk ratio, Relative risk ratio and P values were calculated. A total of 3241 RPL cases and 3205 healthy controls from 21 different case-control studies were analysed. RPL was highly prevalent in mixed population with VEGF-1154G/A and p53 Arg72Pro polymorphisms (70.04% and 66.46% respectively) and in Asian population with VEGF 936C/T polymorphism (53.58%). The homozygous recessive genotypes of VEGF and p53 exhibited significant association between the respective polymorphisms and RPL along with the increased risk of outcome. The current analysis conclusively reports the geographic distribution of the different genetic polymorphisms which shows high association with the progression of RPL. Understanding the spectrum of polymorphisms on different population with the spiral artery remodelling as a risk factor encloses the importance of the vasculature during the pregnancy.

scholarly journals Mycoplasma genitaliumInfection and Female Reproductive Tract Disease: A Meta-analysis

2015 ◽  
Vol 61 (3) ◽  
pp. 418-426 ◽  
Author(s):  
Rebecca Lis ◽  
Ali Rowhani-Rahbar ◽  
Lisa E. Manhart
Keyword(s):  
Reproductive Tract ◽  
Meta Analysis ◽  
Female Reproductive Tract ◽  
Tract Disease

scholarly journals Association among cytotoxic T-lymphocyte antigen 4 gene, rs231775 polymorphism, and recurrent pregnancy loss risk

2019 ◽  
Vol 39 (2) ◽  
Author(s):  
Yonghui Song ◽  
Ying Chen ◽  
Qian Xu
Keyword(s):  
T Lymphocyte ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Cytotoxic T Lymphocyte ◽  
Meta Analysis ◽  
Women Of Childbearing Age ◽  
Case Control Studies ◽  
Childbearing Age ◽  
Lymphocyte Antigen ◽  
Similar Association

Abstract Cytotoxic T-lymphocyte antigen 4 (CTLA-4) is expressed constitutively on regulatory T cells. So far, several studies have focused on association between CTLA-4 gene polymorphisms and recurrent pregnancy loss (RPL). However, above association between the CTLA-4 gene polymorphism and RPL susceptibility is uncertain. Therefore, we performed a timely meta-analysis of all current publications to clarify this relationship. We located articles from the PubMed and Chinese language (WanFang) databases that were published up until July 25, 2018. Finally, we obtained six case–control studies, containing 2405 total cases and 2607 total controls, based on search criteria for abortion susceptibility related to the CTLA-4 +49 G/A polymorphism. The odds ratios (OR) and 95% confidence intervals (CIs) revealed association strengths. There was significantly decreased association between this polymorphism and whole population risk (e.g. AA vs. GG: OR = 0.56, 95% CI = 0.38–0.81, P=0.002). Additionally, in ethnicity subgroups, similar association was found both in China (e.g. AA vs. GG: OR = 0.49, 95% CI = 0.39–0.63, P=0.002) and non-China (e.g. AG vs. GG: OR = 0.46, 95% CI = 0.34–0.63, P<0.001). Current analysis suggested CTLA-4 +49 G/A polymorphism may weakly decrease RPL risk for women of childbearing age.

Subclinical hypothyroidism and thyroid autoimmunity in recurrent pregnancy loss: a systematic review and meta-analysis

2020 ◽  
Vol 113 (3) ◽  
pp. 587-600.e1 ◽  
Author(s):  
Allan C. Dong ◽  
Jessica Morgan ◽  
Monica Kane ◽  
Alex Stagnaro-Green ◽  
Mary D. Stephenson
Keyword(s):  
Systematic Review ◽  
Subclinical Hypothyroidism ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Thyroid Autoimmunity
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