scholarly journals A 3D Printed Vitrification Device for Storage in Cryopreservation Vials

2021 ◽  
Vol 11 (17) ◽  
pp. 7977 ◽  
Author(s):  
Yue Liu ◽  
Andy Lin ◽  
Terrence R. Tiersch ◽  
William Todd Monroe
Keyword(s):  
Low Cost ◽  
Three Dimensional ◽  
Open Loop ◽  
Sperm Cryopreservation ◽  
Sample Handling ◽  
Embryo Freezing ◽  
Open Hardware ◽  
User Communities ◽  
3D Printers ◽  
3D Printed

Sperm cryopreservation by vitrification is a promising approach for small-bodied animals such as zebrafish (Danio rerio). However, most vitrification tools adopted in aquatic research were initially designed for applications other than sperm (such as human embryo freezing) and, thus, pose challenges for adoption to sperm vitrification. Three-dimensional (3D) printing combined with open hardware sharing is an emerging strategy to address challenges in the development of cryopreservation tools. The goal of this study was to develop a 3D printed Vitrification Device for Cryo-Vials (VDCV) that can be integrated with the existing vial storage systems. The VDCV combined the vitrification and handling components to achieve functions of sample handling, vitrification, storage, and identification. The vitrification component featured a base, a stem, and a loop. A total of 36 configurations with various loop lengths (8, 10, and 12 mm); loop widths (2.0, 2.5, 3.0, and 3.5 mm); and support structures (open, transverse, and axial) of the VDCD prototypes were designed, fabricated, and tested. Device handling orientations (horizontal and vertical holding angles prior to and during freezing) were also investigated. Computer simulations estimated that the cooling rate of the samples ranged from 0.6–1.5 × 105 °C/min in all the configurations. Prior to freezing, loops with axial supports produced a minimum of 92% film retention. The overall trends of full vitrification occurrence were observed: horizontal plunging > vertical plunging, and axial support > transverse support and open loop. A loop length of 8 mm had the highest overall vitrification occurrence (86–100%). No significant differences (p = 0.6584) were shown in a volume capacity (5.7–6.0 µL) among the three supporting configurations. A single unit of VDCV can provide loading efficiencies of about 6 × 107 sperm/vial, pooling of samples from 3–6 males/vial, and fertilization for 1800 eggs/vial. The VDCV are low-cost (<$0.5 material cost per unit) and can be customized, standardized, securely labeled, and efficiently stored. The prototypes can be accessed by user communities through open-fabrication file sharing and fabricated with consumer-level 3D printers, thus facilitating community-level standardization.

scholarly journals From tissue to silicon to plastic: three-dimensional printing in comparative anatomy and physiology

2016 ◽  
Vol 3 (3) ◽  
pp. 150643 ◽  
Author(s):  
Henrik Lauridsen ◽  
Kasper Hansen ◽  
Mathias Ørum Nørgård ◽  
Tobias Wang ◽  
Michael Pedersen
Keyword(s):  
Comparative Anatomy ◽  
Low Cost ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Anatomy And Physiology ◽  
3D Space ◽  
3D Printers ◽  
3D Printed ◽  
Written Descriptions ◽  
Animal Anatomy

Comparative anatomy and physiology are disciplines related to structures and mechanisms in three-dimensional (3D) space. For the past centuries, scientific reports in these fields have relied on written descriptions and two-dimensional (2D) illustrations, but in recent years 3D virtual modelling has entered the scene. However, comprehending complex anatomical structures is hampered by reproduction on flat inherently 2D screens. One way to circumvent this problem is in the production of 3D-printed scale models. We have applied computed tomography and magnetic resonance imaging to produce digital models of animal anatomy well suited to be printed on low-cost 3D printers. In this communication, we report how to apply such technology in comparative anatomy and physiology to aid discovery, description, comprehension and communication, and we seek to inspire fellow researchers in these fields to embrace this emerging technology.

scholarly journals Flexible 3D Printed Molds for Educational Use. Digital Fabrication of 3D Typography

2019 ◽  
Vol 15 (13) ◽  
pp. 4 ◽  
Author(s):  
Alejandro Bonnet De León ◽  
Jose Luis Saorin ◽  
Jorge De la Torre-Cantero ◽  
Cecile Meier ◽  
María Cabrera-Pardo
Keyword(s):  
3D Modeling ◽  
Low Cost ◽  
Digital Fabrication ◽  
3D Printer ◽  
School Environments ◽  
Flexible Material ◽  
Educational Environments ◽  
3D Printers ◽  
3D Printed ◽  
The Subject

<p class="0abstract"><span lang="EN-US">One of the drawbacks of using 3D printers in educational environments is that the creation time of each piece is high and therefore it is difficult to manufacture at least one piece for each student. This aspect is important so that each student can feel part of the manufacturing process. To achieve this, 3D printers can be used, not to make pieces, but to make the molds that students use to create replicas. On the other hand, for a mold to be used to make several pieces, it is convenient to make it with flexible material. However, most used material for 3D printers (PLA) is very rigid. To solve this problem, this article designs a methodology that allows the use of low-cost 3D printers (most common in school environments) with flexible material so that each mold can be used to manufacture parts for several students. To print flexible material with low-cost printers, it is necessary to adapt the machine and the print parameters to work properly. This article analyzes the changes to be made with a low cost 3D printer and validates the use of molds in school environments. A pilot test has been carried out with 8 students of the subject of Typography, in the School of Art and Superior of Design of Tenerife. During the activity, the students carried out the process of designing a typography and creating digital molds for 3D printing with flexible material. The designs were made using free 3D modeling programs and low-cost technologies.</span></p>

scholarly journals Three-Dimensional Chipless RFID Tags: Fabrication through Additive Manufacturing

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4740
Author(s):  
Sergio Terranova ◽  
Filippo Costa ◽  
Giuliano Manara ◽  
Simone Genovesi
Keyword(s):  
Radio Frequency Identification ◽  
Low Cost ◽  
Three Dimensional ◽  
Rfid Tags ◽  
Metallic Surfaces ◽  
Data Encoding ◽  
Chipless Rfid ◽  
Frequency Identification ◽  
3D Printed ◽  
Promising Solution

A new class of Radio Frequency IDentification (RFID) tags, namely the three-dimensional (3D)-printed chipless RFID one, is proposed, and their performance is assessed. These tags can be realized by low-cost materials, inexpensive manufacturing processes and can be mounted on metallic surfaces. The tag consists of a solid dielectric cylinder, which externally appears as homogeneous. However, the information is hidden in the inner structure of the object, where voids are created to encrypt information in the object. The proposed chipless tag represents a promising solution for anti-counterfeiting or security applications, since it avoids an unwanted eavesdropping during the reading process or information retrieval from a visual inspection that may affect other chipless systems. The adopted data-encoding algorithm does not rely on On–Off or amplitude schemes that are commonly adopted in the chipless RFID implementations but it is based on the maximization of available states or the maximization of non-overlapping regions of uncertainty. The performance of such class of chipless RFID tags are finally assessed by measurements on real prototypes.

scholarly journals Software-Based Three-Dimensional Deconvolution Microscopy of Cytoskeletal Proteins in Cultured Fibroblast Using Open-Source Software and Open Hardware

2019 ◽  
Vol 5 (12) ◽  
pp. 88
Author(s):  
Kazuo Katoh
Keyword(s):  
Open Source ◽  
Open Source Software ◽  
Laser Scanning ◽  
Low Cost ◽  
Three Dimensional ◽  
Cytoskeletal Proteins ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Actual Measurement ◽  
Open Hardware

As conventional fluorescence microscopy and confocal laser scanning microscopy generally produce images with blurring at the upper and lower planes along the z-axis due to non-focal plane image information, the observation of biological images requires “deconvolution.” Therefore, a microscope system’s individual blur function (point spread function) is determined theoretically or by actual measurement of microbeads and processed mathematically to reduce noise and eliminate blurring as much as possible. Here the author describes the use of open-source software and open hardware design to build a deconvolution microscope at low cost, using readily available software and hardware. The advantage of this method is its cost-effectiveness and ability to construct a microscope system using commercially available optical components and open-source software. Although this system does not utilize expensive equipment, such as confocal and total internal reflection fluorescence microscopes, decent images can be obtained even without previous experience in electronics and optics.

The Sparthan Three-Dimensional Printed Exo-Glove: A Preliminary Evaluation of Performance Via Case Study

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
Tomás A. Georgiou ◽  
Davide Asnaghi ◽  
Alva Liang ◽  
Alice M. Agogino
Keyword(s):  
Light Intensity ◽  
Daily Living ◽  
Low Cost ◽  
Three Dimensional ◽  
Preliminary Evaluation ◽  
Evaluation Of Performance ◽  
3D Printed ◽  
Hand Exoskeleton ◽  
And Performance

This paper describes the development and testing of a low-cost three-dimensional (3D) printed wearable hand exoskeleton to assist people with limited finger mobility and grip strength. The function of the presented orthosis is to support and enable light intensity activities of daily living and improve the ability to grasp and hold objects. The Sparthan Exoskeleton prototype utilizes a cable-driven design applied to individual digits with motors. The initial prototype is presented in this paper along with a preliminary evaluation of durability and performance efficacy.

A Case Study in 3D Printed Porous Ceramics: Infant Incubator Humidification System

2012 ◽  
Author(s):  
Olaf Diegel ◽  
Andrew Withell ◽  
Deon Debeer ◽  
Mark Wu
Keyword(s):  
Material Properties ◽  
Low Cost ◽  
Ceramic Materials ◽  
Porous Ceramics ◽  
Burn Out ◽  
Infant Incubator ◽  
3D Printers ◽  
3D Printed ◽  
Factorial Design Experiment

This paper describes research in adapting 3D printers to operate with low-cost ceramic materials. The components produced with these clay-based ceramic powders can be fired to produce strong, complex and lightweight ceramic parts. The final material properties, including the porosity of the parts, can be controlled through the part design and, potentially, through additives to the material that burn out during firing. The paper begins with a brief description of the 3D printing process and how it can be used with clay powders. It then introduces a factorial design experiment initiated to explore the effect of ingredient and parameter variations on the dimensional stability and material properties of green and fired ceramic parts. It then presents a case study in which 3D printed ceramic parts are used in the humidification system for an infant incubator for developing countries.

scholarly journals Additive Manufactured Waveguide for E-Band Using Ceramic Materials

2021 ◽  
Vol 12 (1) ◽  
pp. 212
Author(s):  
Florian Hubert ◽  
Tobias Bader ◽  
Larissa Wahl ◽  
Andreas Hofmann ◽  
Konstantin Lomakin ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Attenuation Coefficient ◽  
Low Cost ◽  
Ceramic Materials ◽  
Electrical Measurements ◽  
3D Printers ◽  
3D Printed ◽  
Application Fields ◽  
Metallization Process ◽  
Waveguide Geometry

Ceramic materials are chemical- and temperature-resistant and, therefore, enable novel application fields ranging from automotive to aerospace. With this in mind, this contribution focuses on developing an additive manufacturing approach for 3D-printed waveguides made of ceramic materials. In particular, a special design approach for ceramic waveguides, which introduces non-radiating slots into the waveguides sidewalls, and a customized metallization process, are presented. The developed process allows for using conventional stereolithographic desktop-grade 3D-printers. The proposed approach has, therefore, benefits such as low-cost fabrication, moderate handling effort and independence of the concrete waveguide geometry. The performance of a manufactured ceramic WR12 waveguide is compared to a commercial waveguide and a conventionally printed counterpart. For that reason, relevant properties, such as surface roughness and waveguide geometry, are characterized. Parsing the electrical measurements, the ceramic waveguide specimen features an attenuation coefficient of 30–60 dB/m within the E-Band. The measured attenuation coefficient is 200% and 300% higher compared to the epoxy resin and the commercial waveguide and is attributed to the increased surface roughness of the ceramic substrate.

scholarly journals Development of a Low-Cost EEG-Controlled Hand Exoskeleton 3D Printed on Textiles

2021 ◽  
Vol 15 ◽  
Author(s):  
Rommel S. Araujo ◽  
Camille R. Silva ◽  
Severino P. N. Netto ◽  
Edgard Morya ◽  
Fabricio L. Brasil
Keyword(s):  
Low Cost ◽  
Three Dimensional ◽  
Training Session ◽  
Life Quality ◽  
Motor Deficits ◽  
Stroke Survivors ◽  
Motor Rehabilitation ◽  
3D Printed ◽  
Hand Exoskeleton ◽  
Robotic Devices

Stroke survivors can be affected by motor deficits in the hand. Robotic equipment associated with brain–machine interfaces (BMI) may aid the motor rehabilitation of these patients. BMIs involving orthotic control by motor imagery practices have been successful in restoring stroke patients' movements. However, there is still little acceptance of the robotic devices available, either by patients and clinicians, mainly because of the high costs involved. Motivated by this context, this work aims to design and construct the Hand Exoskeleton for Rehabilitation Objectives (HERO) to recover extension and flexion movements of the fingers. A three-dimensional (3D) printing technique in association with textiles was used to produce a lightweight and wearable device. 3D-printed actuators have also been designed to reduce equipment costs. The actuator transforms the torque of DC motors into linear force transmitted by Bowden cables to move the fingers passively. The exoskeleton was controlled by neuroelectric signal—electroencephalography (EEG). Concept tests were performed to evaluate control performance. A healthy volunteer was submitted to a training session with the exoskeleton, according to the Graz-BCI protocol. Ergonomy was evaluated with a two-dimensional (2D) tracking software and correlation analysis. HERO can be compared to ordinary clothing. The weight over the hand was around 102 g. The participant was able to control the exoskeleton with a classification accuracy of 91.5%. HERO project resulted in a lightweight, simple, portable, ergonomic, and low-cost device. Its use is not restricted to a clinical setting. Thus, users will be able to execute motor training with the HERO at hospitals, rehabilitation clinics, and at home, increasing the rehabilitation intervention time. This may support motor rehabilitation and improve stroke survivors life quality.

scholarly journals An overview on 3D Printed Medicine

2021 ◽  
Vol 18 (1) ◽  
pp. 07-13
Author(s):  
Neha Thakur ◽  
Hari Murthy
Keyword(s):  
3D Printing ◽  
Fused Deposition Modeling ◽  
Low Cost ◽  
Selective Laser Sintering ◽  
Three Dimensional ◽  
Herbal Medicines ◽  
Fused Deposition ◽  
Digitally Controlled ◽  
3D Printed ◽  
Semi Solid

Three-dimensional printing (3DP) is a digitally-controlled additive manufacturing technique used for fast prototyping. This paper reviews various 3D printing techniques like Selective Laser Sintering (SLS), Fused Deposition Modeling, (FDM), Semi-solid extrusion (SSE), Stereolithography (SLA), Thermal Inkjet (TIJ) Printing, and Binder jetting 3D Printing along with their application in the field of medicine. Normal medicines are based on the principle of “one-size-fits-all”. This is not true always, it is possible medicine used for curing one patient is giving some side effects to another. To overcome this drawback “3D Printed medicines” are developed. In this paper, 3D printed medicines forming different Active Pharmaceutical Ingredients (API) are reviewed. Printed medicines are capable of only curing the diseases, not for the diagnosis. Nanomedicines have “theranostic” ability which combines therapeutic and diagnostic. Nanoparticles are used as the drug delivery system (DDS) to damaged cells’ specific locations. By the use of nanomedicine, the fast recovery of the disease is possible. The plant-based nanoparticles are used with herbal medicines which give low-cost and less toxic medication called nanobiomedicine. 4D and 5D printing technology for the medical field are also enlightened in this paper.

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