scholarly journals Methods for Studying Bacterial–Fungal Interactions in the Microenvironments of Soil

2021 ◽  
Vol 11 (19) ◽  
pp. 9182
Author(s):  
Edoardo Mandolini ◽  
Maraike Probst ◽  
Ursula Peintner
Keyword(s):  
Experimental Data ◽  
Experimental Design ◽  
Environmental Heterogeneity ◽  
Functional Prediction ◽  
Cell Level ◽  
Soil Matrix ◽  
Ecosystem Level ◽  
Physical Interactions ◽  
Fungal Interactions

Due to their small size, microorganisms directly experience only a tiny portion of the environmental heterogeneity manifested in the soil. The microscale variations in soil properties constrain the distribution of fungi and bacteria, and the extent to which they can interact with each other, thereby directly influencing their behavior and ecological roles. Thus, to obtain a realistic understanding of bacterial–fungal interactions, the spatiotemporal complexity of their microenvironments must be accounted for. The objective of this review is to further raise awareness of this important aspect and to discuss an overview of possible methodologies, some of easier applicability than others, that can be implemented in the experimental design in this field of research. The experimental design can be rationalized in three different scales, namely reconstructing the physicochemical complexity of the soil matrix, identifying and locating fungi and bacteria to depict their physical interactions, and, lastly, analyzing their molecular environment to describe their activity. In the long term, only relevant experimental data at the cell-to-cell level can provide the base for any solid theory or model that may serve for accurate functional prediction at the ecosystem level. The way to this level of application is still long, but we should all start small.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Transmitting Knowledge across Divides

2016 ◽  
Vol 46 (3) ◽  
pp. 313-359 ◽  
Author(s):  
Marta Jordi Taltavull
Keyword(s):  
Experimental Data ◽  
Quantum Mechanics ◽  
Quantum Theory ◽  
Scientific Understanding ◽  
Resonance Model ◽  
Optical Dispersion ◽  
History Of

One model, the resonance model, shaped scientific understanding of optical dispersion from the early 1870s to the 1920s, persisting across dramatic changes in physical conceptions of light and matter. I explore the ways in which the model was transmitted across these conceptual divides by analyzing the use of the model both in the development of theories of optical dispersion and in the interpretation of experimental data. Crucial to this analysis is the integration of the model into quantum theory because of the conceptual incompatibility between the model and quantum theory. What is more, a quantum understanding of optical dispersion set the grounds for the emergence of the first theories of quantum mechanics in 1925. A long-term history of the model’s transmission from the 1870s to the 1920s illuminates the ways in which the continuity of knowledge is possible across these discontinuities.

Insights into associative long-term potentiation from computational models of NMDA receptor-mediated calcium influx and intracellular calcium concentration changes

1990 ◽  
Vol 63 (5) ◽  
pp. 1148-1168 ◽  
Author(s):  
W. R. Holmes ◽  
W. B. Levy
Keyword(s):  
Experimental Data ◽  
Nmda Receptor ◽  
Nmda Receptors ◽  
Dendritic Spine ◽  
Long Term Potentiation ◽  
Input Frequency ◽  
Spine Head ◽  
Term Potentiation ◽  
Concentration Changes

1. Because induction of associative long-term potentiation (LTP) in the dentate gyrus is thought to depend on Ca2+ influx through channels controlled by N-methyl-D-aspartate (NMDA) receptors, quantitative modeling was performed of synaptically mediated Ca2+ influx as a function of synaptic coactivation. The goal was to determine whether Ca2+ influx through NMDA-receptor channels was, by itself, sufficient to explain associative LTP, including control experiments and the temporal requirements of LTP. 2. Ca2+ influx through NMDA-receptor channels was modeled at a synapse on a dendritic spine of a reconstructed hippocampal dentate granule cell when 1-115 synapses on spines at different dendritic locations were activated eight times at frequencies of 10-800 Hz. The resulting change in [Ca2+] in the spine head was estimated from the Ca2+ influx with the use of a model of a dendritic spine that included Ca2+ buffers, pumps, and diffusion. 3. To use a compelling model of synaptic activation, we developed quantitative descriptions of the NMDA and non-NMDA receptor-mediated conductances consistent with available experimental data. The experimental data reported for NMDA and non-NMDA receptor-channel properties and data from other non-LTP experiments that separated the NMDA and non-NMDA receptor-mediated components of synaptic events proved to be limiting for particular synaptic variables. Relative to the non-NMDA glutamate-type receptors, 1) the unbinding of transmitter from NMDA receptors had to be slow, 2) the transition from the bound NMDA receptor-transmitter complex to the open channel state had to be even slower, and 3) the average number of NMDA-receptor channels at a single activated synapse on a single spine head that were open and conducting at a given moment in time had to be very small (usually less than 1). 4. With the use of these quantitative synaptic conductance descriptions. Ca2+ influx through NMDA-receptor channels at a synapse was computed for a variety of conditions. For a constant number of pulses, Ca2+ influx was calculated as a function of input frequency and the number of coactivated synapses. When few synapses were coactivated, Ca2+ influx was small, even for high-frequency activation. However, with larger numbers of coactivated synapses, there was a steep increase in Ca2+ influx with increasing input frequency because of the voltage-dependent nature of the NMDA receptor-mediated conductance. Nevertheless, total Ca2+ influx was never increased more than fourfold by increasing input frequency or the number of coactivated synapses.(ABSTRACT TRUNCATED AT 400 WORDS)

Developing an Adaptive Management approach to prescribed burning: a long-term heathland conservation experiment in north-west Italy

2009 ◽  
Vol 18 (6) ◽  
pp. 727 ◽  
Author(s):  
Davide Ascoli ◽  
Rachele Beghin ◽  
Riccardo Ceccato ◽  
Alessandra Gorlier ◽  
Giampiero Lombardi ◽  
...  
Keyword(s):  
Experimental Design ◽  
Adaptive Management ◽  
Prescribed Burning ◽  
Fire Regime ◽  
Management Approach ◽  
Detailed Measurement ◽  
Research Issues ◽  
North West ◽  
Heathland Management

Calluna vulgaris-dominated heathlands are globally important habitats and extremely scarce outside of north-west Europe. Rotational fire, grazing and cutting by local farmers were dominant features of past heathland management throughout Europe but have been abandoned, altering the historical fire regime and habitat structure. We briefly review research on Calluna heathland conservation management and provide the background and methodology for a long-term research project that will be used to define prescribed fire regimes in combination with grazing and cutting, for management of Calluna heathlands in north-west Italy. We outline the ecological and research issues that drive the fire experiment, making explicit the experimental design and the hypotheses that will be tested. We demonstrate how Adaptive Management can be used to inform decisions about the nature of fire prescriptions where little formal knowledge exists. Experimental plots ranging from 600 to 2500 m2 are treated according to one of eight alternative treatments (various combinations of fire, grazing and cutting), each replicated four times. To date, all treatments have been applied for 4 years, from 2005 to 2008, and a continuation is planned. Detailed measurement of fire characteristics is made to help interpret ecological responses at a microplot scale. The results of the experiment will be fed back into the experimental design and used to inform heathland management practice in north-west Italy.

Oscillations and Long-Lasting Correlations in a Model of the Lateral Geniculate Nucleus and Visual Cortex

2000 ◽  
Vol 84 (4) ◽  
pp. 1863-1868 ◽  
Author(s):  
Kyle L. Kirkland ◽  
Adam M. Sillito ◽  
Helen E. Jones ◽  
David C. West ◽  
George L. Gerstein
Keyword(s):  
Experimental Data ◽  
Visual Cortex ◽  
Cell Membrane ◽  
Lateral Geniculate Nucleus ◽  
Feedback Inhibition ◽  
Frequency Range ◽  
Lateral Geniculate ◽  
Low Threshold ◽  
Thalamocortical System

We have previously developed a model of the corticogeniculate system to explore cortically induced synchronization of lateral geniculate nucleus (LGN) neurons. Our model was based on the experiments of Sillito et al. Recently Brody discovered that the LGN events found by Sillito et al. correlate over a much longer period of time than expected from the stimulus-driven responses and proposed a cortically induced slow covariation in LGN cell membrane potentials to account for this phenomenon. We have examined the data from our model, and we found, to our surprise, that the model shows the same long-term correlation. The model's behavior was the result of a previously unsuspected oscillatory effect, not a slow covariation. The oscillations were in the same frequency range as the well-known spindle oscillations of the thalamocortical system. In the model, the strength of feedback inhibition from the cortex and the presence of low-threshold calcium channels in LGN cells were important. We also found that by making the oscillations more pronounced, we could get a better fit to the experimental data.

scholarly journals Parameters of temperature disturbance triggering the propagation of the quench front on an extremely overheated surface

2021 ◽  
Vol 2119 (1) ◽  
pp. 012079
Author(s):  
I P Starodubtseva ◽  
A N Pavlenko
Keyword(s):  
Experimental Data ◽  
High Temperature ◽  
Front Propagation ◽  
Solid Contact ◽  
Temperature Disturbance ◽  
Metal Plates ◽  
Leidenfrost Temperature ◽  
Dynamical Pattern ◽  
Temperature Surface

Abstract The results of computational experiments simulating the triggering of the quench front propagation on the superheated vertically oriented metal plates are presented. The plates are quenched by a gravitationally flowing down liquid nitrogen film. The temperature of the test samples at the beginning of the process was higher than the critical temperature and the Leidenfrost temperature, which means that direct long-term liquid-solid contact is impossible. For this reason, the front is initially motionless. As a result of numerical simulation, a dynamic pattern of the quench front propagation on a high-temperature surface was obtained. Analysis of the results allowed to find the realistic values of heat sink into the cooling medium, as well as the parameters of the local temperature disturbance, its spatial extent and amplitude, at which the conditions are created for triggering the process of quench front propagation on the high-temperature surface. Direct comparison of the numerical simulations results with experimental data on the velocity, geometry of the quench front and on the dynamical pattern of the process confirmed the reliability of the results obtained.

scholarly journals Cheaters shape the evolution of phenotypic heterogeneity in Bacillus subtilis biofilms

2018 ◽  
Author(s):  
Marivic Martin ◽  
Anna Dragoš ◽  
Simon B. Otto ◽  
Daniel Schäfer ◽  
Susanne Brix ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Prolonged Exposure ◽  
Adaptive Strategy ◽  
Phenotypic Heterogeneity ◽  
Structural Proteins ◽  
Cell Level ◽  
General Adaptation ◽  
Matrix Production ◽  
Matrix Components

ABSTRACTBiofilms are closely packed cells held and shielded by extracellular matrix composed of structural proteins and exopolysaccharides (EPS). As matrix components are costly to produce and shared within the population, EPS-deficient cells can act as cheaters by gaining benefits from the cooperative nature of EPS producers. Remarkably, genetically programmed EPS producers can also exhibit phenotypic heterogeneity at single cell level. Previous studies have shown that spatial structure of biofilms limits the spread of cheaters, but the long-term influence of cheating on biofilm evolution is not well understood. Here, we examine the influence of EPS non-producers on evolution of matrix production within the populations of EPS producers in a model biofilm-forming bacterium, Bacillus subtilis. We discovered that general adaptation to biofilm lifestyle leads to an increase in phenotypical heterogeneity of eps expression. Apparently, prolonged exposure to EPS-deficient cheaters, may result in different adaptive strategy, where eps expression increases uniformly within the population. We propose a molecular mechanism behind such adaptive strategy and demonstrate how it can benefit the EPS-producers in the presence of cheaters. This study provides additional insights on how biofilms adapt and respond to stress caused by exploitation in long-term scenario.

scholarly journals Effect of Solar Modulation on the Low Energy Sea Level Muon Spectrum Near the Geomagnetic Equator

1978 ◽  
Vol 31 (5) ◽  
pp. 451 ◽  
Author(s):  
DP Bhattacharyya
Keyword(s):  
Experimental Data ◽  
Sea Level ◽  
Low Energy ◽  
Solar Modulation ◽  
Recent Experimental Data ◽  
Geomagnetic Equator ◽  
Muon Spectrum ◽  
Muon Intensity ◽  
Theoretical Results

A study is made of the influence of long-term solar modulation on the low energy sea level muon spectrum near the geomagnetic equator. Recent experimental data are compared with theoretical results calculated from the phenomenological model of Allkofer and Dau. It is suggested that the observed enhancement in the muon intensity is mainly due to a shift in the solar potential.

scholarly journals Metabolic plasticity can amplify ecosystem responses to global warming

2021 ◽  
Author(s):  
Rebecca Kordas ◽  
Samraat Pawar ◽  
Guy Woodward ◽  
Eoin O'Gorman
Keyword(s):  
Global Warming ◽  
Body Size ◽  
Predictive Models ◽  
Chronic Exposure ◽  
Ecosystem Respiration ◽  
Short Term ◽  
Ecosystem Responses ◽  
Metabolic Plasticity ◽  
Ecosystem Level

Abstract Organisms have the capacity to alter their physiological response to warming through acclimation or adaptation, but empirical evidence for this metabolic plasticity across species within food webs is lacking, and a generalisable framework does not exist for modelling its ecosystem-level consequences. Here we show that the ability of organisms to raise their metabolic rate following chronic exposure to warming decreases with increasing body size. Chronic exposure to higher temperatures also increases the sensitivity of organisms to short-term warming, irrespective of their body size. A mathematical model parameterised with these findings shows that metabolic plasticity could account for an additional 60% of ecosystem energy flux with just +2 °C of warming. This could explain why ecosystem respiration continues to rise in long-term warming experiments and highlights the need to embed metabolic plasticity in predictive models of global warming impacts on ecosystems.

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