scholarly journals Virus-Like Particle Production in Atmospheric Eubacteria Isolates

Atmosphere ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 417 ◽  
Author(s):  
Teigell-Perez ◽  
Gonzalez-Martin ◽  
Valladares ◽  
Smith ◽  
Griffin

Culturable eubacterial isolates were collected at various altitudes in Earth’s atmosphere, including ~1.5 m above ground in Tallahassee, FL, USA; ~10.0 m above sea level over the mid-Atlantic ridge (~15° N); ~ 20 km above ground over the continental United States; ~20 km above sea level over the Pacific Ocean near southern California; and from the atmosphere of Carlsbad Cavern, Carlsbad Cavern National Park, NM, USA. Isolates were screened for the presence of inducible virus-like particles (VLP) through the use of mitomycin C and epifluorescent direct counts. We determined that 92.7% of the isolates carried inducible VLP counts in exposed versus non-exposed culture controls and that the relationship was statistically significant. Further statistical analyses revealed that the number of isolates that demonstrated VLP production did not vary among collection sites. These data demonstrate a high prevalence of VLP generation in isolates collected in the lower atmosphere and at extreme altitudes. They also show that species of eubacteria that are resistant to the rigors of atmospheric transport play a significant role in long-range atmospheric inter- and intra-continental dispersion of VLP and that long-range atmospheric transport of VLP may enhance rates of evolution at the microbial scale in receiving environments.

Author(s):  
Benjamin Nogueda-Torres ◽  
Oziel D Montañez-Valdez ◽  
J G Michel-Parra ◽  
Diana M Martínez-Grant ◽  
J Alejandro Martínez-Ibarra

Abstract Chagas disease is a very important vector-borne disease in México, and Triatoma dimidiata sensu stricto (Latreille) is one of the most important vectors of Trypanosoma cruzi Chagas, mainly in southern and central states. In the Pacific Coast states with the highest prevalence of human T. cruzi infection, T. dimidiata s. s. is considered as a secondary vector. However, the vectorial capacity of those populations has not been studied. Therefore, the vector characteristics of three populations of T. dimidiata s. s. in western México were evaluated in this study. The populations were maintained in the laboratory at 27 ± 1°C and 75% ± 5% RH with a 12:12 h (light:dark) regime, fed on rabbits in a fortnight basis. The development times were short (172–238 d), and the number of bloodmeals to molt was low (11). Mortality was moderate (36–45%), the onset of feeding was relatively rapid (0.5–1.7 min), and feedings were extended (>15 min). More than 40% of individuals in most instars defecated in one of three categories: <1 min when feeding (5–37.9%), immediately after feeding (9–28.6%), or in <1 min post feeding (7–25.8%). The median number of laid eggs was high (over 2.5) in the three populations, as were the egg eclosion rates (>86%). Thus, the T. dimidiata s. s. in the three populations are potentially efficient vectors of T. cruzi and could contribute to the high prevalence of infection in human populations in western México.


2021 ◽  
pp. 1-52
Author(s):  
Yi Jin ◽  
Xuebin Zhang ◽  
John A. Church ◽  
Xianwen Bao

AbstractProjections of future sea-level changes are usually based on global climate models (GCMs). However, the changes in shallow coastal regions, like the marginal seas near China, cannot be fully resolved in GCMs. To improve regional sea-level simulations, a high-resolution (~8 km) regional ocean model is set up for the marginal seas near China for both the historical (1994-2015) and future (2079-2100) periods under representative concentration pathways (RCPs) 4.5 and 8.5. The historical ocean simulations are evaluated at different spatiotemporal scales, and the model is then integrated for the future period, driven by projected monthly climatological climate change signals from 8 GCMs individually via both surface and open boundary conditions. The downscaled ocean changes derived by comparing historical and future experiments reveal greater spatial details than those from GCMs, e.g., a low dynamic sea level (DSL) centre of -0.15 m in the middle of the South China Sea (SCS). As a novel test, the downscaled results driven by the ensemble mean forcings are almost identical with the ensemble average results from individually downscaled cases. Forcing of the DSL change and increased cyclonic circulation in the SCS are dominated by the climate change signals from the Pacific, while the DSL change in the East China marginal seas is caused by both local atmosphere forcing and signals from the Pacific. The method of downscaling developed in this study is a useful modelling protocol for adaptation and mitigation planning for future oceanic climate changes.


2021 ◽  
Author(s):  
Daniel R. Muhs

Abstract. The primary last interglacial, marine isotope substage (MIS) 5e records on the Pacific Coast of North America, from Washington (USA) to Baja California Sur (Mexico), are found in the deposits of erosional marine terraces. Warmer coasts along the southern Golfo de California host both erosional marine terraces and constructional coral reef terraces. Because the northern part of the region is tectonically active, MIS 5e terrace elevations vary considerably, from a few meters above sea level to as much as 70 m above sea level. The primary paleo-sea level indicator is the shoreline angle, the junction of the wave-cut platform with the former sea cliff, which forms very close to mean sea level. Most areas on the Pacific Coast of North America have experienced uplift since MIS 5e time, but the rate of uplift varies substantially as a function of tectonic setting. Chronology in most places is based on uranium-series ages of the solitary coral Balanophyllia elegans (erosional terraces) or the colonial corals Porites and Pocillopora (constructional reefs). In areas lacking corals, correlation to MIS 5e can sometimes be accomplished using amino acid ratios of fossil mollusks, compared to similar ratios in mollusks that also host dated corals. U-series analyses of corals that have experienced largely closed-system histories range from ~124 to ~118 ka, in good agreement with ages from MIS 5e reef terraces elsewhere in the world. There is no geomorphic, stratigraphic, or geochronology evidence for more than one high-sea stand during MIS 5e on the Pacific Coast of North America. However, in areas of low uplift rate, the outer parts of MIS 5e terraces apparently were re-occupied by the high-sea stand at ~100 ka (MIS 5c), evident from mixes of coral ages and mixes of molluscan faunas with differing thermal aspects. This sequence of events took place because glacial isostatic adjustment processes acting on North America resulted in regional high-sea stands at ~100 ka and ~80 ka that were higher than is the case in far-field regions, distant from large continental ice sheets. During MIS 5e time, sea surface temperatures (SST) off the Pacific Coast of North America were higher than is the case at present, evident from extralimital southern species of mollusks found in dated deposits. Apparently no wholesale shifts in faunal provinces took place, but in MIS 5e time, some species of bivalves and gastropods lived hundreds of kilometers north of their present northern limits, in good agreement with SST estimates derived from foraminiferal records and alkenone-based reconstructions in deep-sea cores. Because many areas of the Pacific Coast of North America have been active tectonically for much or all of the Quaternary, many earlier interglacial periods are recorded as uplifted, higher elevation terraces. In addition, from southern Oregon to northern Baja California, there are U-series-dated corals from marine terraces that formed ~80 ka, during MIS 5a. In contrast to MIS 5e, these terrace deposits host molluscan faunas that contain extralimital northern species, indicating cooler SST at the end of MIS 5. Here I present a standardized database of MIS 5e sea-level indicators along the Pacific Coast of North America and the corresponding dated samples. The database is available in Muhs (2021)  [https://doi.org/10.5281/zenodo.5557355].


2014 ◽  
Vol 7 (5) ◽  
pp. 4623-4657
Author(s):  
M. Mech ◽  
E. Orlandi ◽  
S. Crewell ◽  
F. Ament ◽  
L. Hirsch ◽  
...  

Abstract. An advanced package of microwave remote sensing instrumentation has been developed for the operation on the new German High Altitude LOng range research aircraft (HALO). The HALO Microwave Package, HAMP, consists of two nadir looking instruments: a cloud radar at 36 GHz and a suite of passive microwave radiometers with 26 frequencies in different bands between 22.24 and 183.31 ± 12.5 GHz. We present a description of HAMP's instrumentation together with an illustration of its potential. To demonstrate this potential synthetic measurements for the implemented passive microwave frequencies and the cloud radar based on cloud resolving and radiative transfer model calculations were performed. These illustrate the advantage of HAMP's chosen frequency coverage, which allows for improved detection of hydrometeors both via the emission and scattering of radiation. Regression algorithms compare HAMP retrieval with standard satellite instruments from polar orbiters and show its advantages particularly for the lower atmosphere with a reduced root mean square error by 5 and 15% for temperature and humidity, respectively. HAMP's main advantage is the high spatial resolution of about 1 km which is illustrated by first measurements from test flights. Together these qualities make it an exciting tool for gaining better understanding of cloud processes, testing retrieval algorithms, defining future satellite instrument specifications, and validating platforms after they have been placed in orbit.


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