Brewing Characteristics of the Maltotriose-Positive Yeast Zygotorulaspora florentina Isolated from Oak

The use of wild yeasts in fermentation is becoming a viable option for the differentiation of beers. To achieve good fermentation rates and alcohol yields, however, such yeasts must have the ability to utilize the wort sugars maltose and maltotriose, a relatively rare trait amongst non-domesticated yeasts. Zygotorulaspora florentina is a species with the ability to utilize both sugars, and was evaluated here with respect to its brewing potential. The strain studied (VTT C-201041) was isolated from bark of an oak tree (Quercus robur) in Espoo, Finland. The fermentation performance of the strain was compared to that of two ale yeasts as well as the species type strain (VTT C-94199). Both Z. florentina strains fermented wort efficiently (apparent attenuation levels >77%). While the type strain had the highest yield, the Finnish strain produced more volatile aroma compounds. The species is capable of decarboxylating ferulic acid to produce the spice/clove-like compound 4-vinylguaiacol, which was present in beers at a concentration above the typical flavor threshold. The characteristic flavor of 4-vinylguaiacol was not however perceptible in taste trials, possibly due to the masking effect of other compounds. The potential of this species for industrial application is discussed, particularly in relation to its apparent ethanol sensitivity.

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Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64242-0 ◽

2006 ◽

Vol 56 (8) ◽

pp. 1777-1782 ◽

Cited By ~ 78

Author(s):

Hang-Yeon Weon ◽

Byung-Yong Kim ◽

Seung-Hee Yoo ◽

Seon-Young Lee ◽

Soon-Wo Kwon ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Dna Hybridization ◽

Type Strain ◽

Sequence Similarity ◽

Rrna Gene ◽

Separate Species ◽

Phenotypic Data ◽

Species Type ◽

Korean Ginseng

Two novel strains, GR20-10T and GR20-13T, were isolated from soil using R2A medium. The soil sample was collected from a field in the Yeongju region of Korea that was cultivated with Korean ginseng. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains formed a cluster with several uncultured bacterial clones and with Flexibacter filiformis, Flexibacter sancti, Flexibacter japonensis, Cytophaga arvensicola and Flavobacterium ferrugineum (recently reclassified as Terrimonas ferruginea) in the phylum Bacteroidetes. The level of 16S rRNA gene sequence similarity between the two novel strains was 98.9 %. Isolates GR20-10T and GR20-13T showed the highest sequence similarities to Flexibacter japonensis IFO 16041T (91.8 and 91.9 %, respectively) and T. ferruginea ATCC 13524T (90.4 and 90.6 %, respectively). The whole-cell fatty acid profiles of the two isolates were similar and their major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. The major isoprenoid quinone of both strains was MK-7. The G+C contents of GR20-10T and GR20-13T were 45.8 and 44.3 mol%, respectively. DNA–DNA hybridization (57 % DNA–DNA hybridization value) and phenotypic data indicated that strains GR20-10T and GR20-13T each belong to a separate species. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains GR20-10T and GR20-13T represent two novel species in a novel genus in the phylum Bacteroidetes; the names Niastella koreensis gen. nov., sp. nov. (the type species; type strain GR20-10T=KACC 11465T=DSM 17620T) and Niastella yeongjuensis sp. nov. (type strain GR20-13T=KACC 11466T=DSM 17621T) are proposed.

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Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64207-0 ◽

2006 ◽

Vol 56 (9) ◽

pp. 2135-2146 ◽

Cited By ~ 169

Author(s):

Niels Nørskov-Lauritsen ◽

Mogens Kilian

Keyword(s):

Type Strain ◽

Aggregatibacter Actinomycetemcomitans ◽

Actinobacillus Actinomycetemcomitans ◽

Strain Atcc ◽

Nicotinamide Phosphoribosyltransferase ◽

Aggregatibacter Aphrophilus ◽

The Family ◽

Haemophilus Aphrophilus ◽

Species Type

The aim of this study was to reinvestigate the relationships and the generic affiliations of the species Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis. The nicotinamide phosphoribosyltransferase gene (nadV) conferring V factor-independent growth was identified in Haemophilus aphrophilus. The gene encodes a polypeptide of 462 amino acids that shows 74.5 % amino acid sequence identity to the corresponding enzyme from Actinobacillus actinomycetemcomitans. Ten isolates of Haemophilus paraphrophilus all carried a nadV pseudogene. DNA from Haemophilus aphrophilus was able to transform Haemophilus paraphrophilus into the NAD-independent phenotype. The transformants carried a full-length nadV inserted in the former locus of the pseudogene. The DNA–DNA relatedness between the type strains of Haemophilus aphrophilus and Haemophilus paraphrophilus was 77 %. We conclude that the division into two species Haemophilus aphrophilus and Haemophilus paraphrophilus is not justified and that Haemophilus paraphrophilus should be considered a later heterotypic synonym of Haemophilus aphrophilus. Forty strains of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus segnis were investigated by multilocus sequence analysis. The 40 strains form a monophyletic group clearly separate from other evolutionary lineages of the family Pasteurellaceae. We propose the transfer of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus segnis to a new genus Aggregatibacter gen. nov. as Aggregatibacter actinomycetemcomitans comb. nov. (the type species; type strain ATCC 33384T=CCUG 13227T=CIP 52.106T=DSM 8324T=NCTC 9710T), Aggregatibacter aphrophilus comb. nov. (type strain ATCC 33389T=CCUG 3715T=CIP 70.73T=NCTC 5906T) and Aggregatibacter segnis comb. nov. (type strain HK316T=ATCC 33393T=CCUG 10787T=CCUG 12838T=CIP 103292T=NCTC 10977T). The species of the genus Aggregatibacter are independent of X factor and variably dependent on V factor for growth in vitro.

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Complete and Circularized Genome Assemblies of the Kroppenstedtia eburnea Genus Type Strain and the Kroppenstedtia pulmonis Species Type Strain with MiSeq and MinION Sequence Data

Microbiology Resource Announcements ◽

10.1128/mra.00650-20 ◽

2020 ◽

Vol 9 (44) ◽

Author(s):

Christopher A. Gulvik ◽

Dhwani Batra ◽

Lori A. Rowe ◽

Milli Sheth ◽

Ben W. Humrighouse ◽

...

Keyword(s):

Type Strain ◽

Sequence Data ◽

Gene Clusters ◽

Illumina Miseq ◽

Putative Gene ◽

Content Type ◽

Oxford Nanopore ◽

Species Type ◽

Genome Assemblies ◽

Oxford Nanopore Technologies

ABSTRACT Kroppenstedtia eburnea DSM 45196T and Kroppenstedtia pulmonis W9323T are aerobic, Gram-positive, filamentous, chemoorganotrophic thermoactinomycetes. Here, we report on the complete and circular genome assemblies generated using Illumina MiSeq and Oxford Nanopore Technologies MinION reads. Putative gene clusters predicted to be involved in the production of secondary metabolites were also identified.

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Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64115-0 ◽

2006 ◽

Vol 56 (5) ◽

pp. 1117-1121 ◽

Cited By ~ 82

Author(s):

Cheng-Hui Xie ◽

Akira Yokota

Keyword(s):

Type Strain ◽

Type Species ◽

New Genus ◽

Single Cells ◽

Garden Soil ◽

Rrna Gene ◽

Phenotypic Characteristics ◽

Separate Species ◽

Species Type ◽

The 16S Rrna Gene

Strain DYT, which was isolated from garden soil in Japan, was subjected to a polyphasic taxonomic study. Sequence analysis of the 16S rRNA gene and the GyrB protein revealed that the closest relative of strain DYT was [Flavobacterium] ferrugineum Sickles and Shaw 1934, with 94.8 and 90.1 % similarity, respectively. The two strains had similar chemotaxonomic characteristics, with menaquinone 7 as the major quinone system, 47.2–48.9 mol% DNA G+C content and 15 : 0 iso, 15 : 1 iso, 17 : 0 iso 3-OH and summed feature 3 as the major fatty acids. Based on genotypic and phenotypic characteristics, [Flavobacterium] ferrugineum IAM 15098T could be clearly differentiated from other members of the genus Flavobacterium. Strain DYT and [Flavobacterium] ferrugineum IAM 15098T could be easily distinguished from neighbouring taxa by morphological features (non-motile, non-gliding and non-filamentous single cells). Therefore, it is proposed that [Flavobacterium] ferrugineum IAM 15098T and strain DYT represent two separate species of a new genus, Terrimonas gen. nov., with the names Terrimonas ferruginea comb. nov. (type species; type strain IAM 15098T=ATCC 13524T) and Terrimonas lutea sp. nov. (type strain DYT=IAM 15284T=CCTCC AB205006T), respectively.

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Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63397-0 ◽

2005 ◽

Vol 55 (3) ◽

pp. 1039-1050 ◽

Cited By ~ 45

Author(s):

R. N. Allan ◽

L. Lebbe ◽

J. Heyrman ◽

P. De Vos ◽

C. J. Buchanan ◽

...

Keyword(s):

Glutamic Acid ◽

Type Strain ◽

Novel Species ◽

Phenotypic Characterization ◽

Uptake System ◽

North West ◽

The North ◽

Victoria Land ◽

Geothermal Soils ◽

Species Type

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that l-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for l-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of l-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T=CIP 108308T), respectively.

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Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63527-0 ◽

2005 ◽

Vol 55 (4) ◽

pp. 1471-1486 ◽

Cited By ~ 164

Author(s):

John P. Bowman ◽

David S. Nichols

Keyword(s):

Sea Ice ◽

Type Strain ◽

Novel Species ◽

Single Species ◽

Distinct Species ◽

Strictly Aerobic ◽

Taxonomic Analysis ◽

Sea Ice Algae ◽

The Family ◽

Species Type

Several orange- and yellow-pigmented, halophilic, strictly aerobic, chemoheterotrophic, Gram-negative strains were isolated during investigations of maritime Antarctic habitats, including coastal fast sea-ice brine and algae, crustaceans and quartz stone sublithic cyanobacterial biofilms. Isolates investigated in this study belonged to the marine clade of the family Flavobacteriaceae and represented lineages that were either distinct from species with validly published names or appeared to be distinct species within existing genera. A polyphasic taxonomic analysis demonstrated the novelty of these strains, and several new taxa are proposed. Strains from quartz stone sublithic communities were grouped into two new genera designated Subsaximicrobium gen. nov. and Subsaxibacter gen. nov. The genus Subsaximicrobium included the species Subsaximicrobium wynnwilliamsii sp. nov. (type species; type strain G#7T=ACAM 1070T=CIP 108525T) and Subsaximicrobium saxinquilinus sp. nov. (type strain Y4-5T=ACAM 1063T=CIP 108526T). The genus Subsaxibacter contained a single species designated Subsaxibacter broadyi sp. nov. (type strain P7T=ACAM 1064T=CIP 108527T). A novel bacterial strain isolated from the lake-dwelling, calanoid copepod Paralabidocera antarctica was given the name Lacinutrix copepodicola gen. nov., sp. nov. (type strain DJ3T=ACAM 1055T=CIP 108538T). Four novel species of the genus Bizionia were discovered, Bizionia algoritergicola sp. nov. (type strain APA-1T=ACAM 1056T=CIP 108533T) and Bizionia myxarmorum sp. nov. (type strain ADA-4T=ACAM 1058T=CIP 108535T), which were isolated from the carapace surfaces of sea-ice algae-feeding amphipods, and Bizionia gelidisalsuginis sp. nov. (type strain IC164T=ACAM 1057T=CIP 108536T) and Bizionia saleffrena sp. nov. (type strain HFDT=ACAM 1059T=CIP 108534T), which were isolated from sea-ice brines. Several other novel species were also isolated from sea-ice samples, including two novel species of the genus Gelidibacter, Gelidibacter gilvus sp. nov. (type strain IC158T=ACAM 1054T=CIP 108531T) and Gelidibacter salicanalis sp. nov. (type strain IC162T=ACAM 1053T=CIP 108532T), as well as three novel species of the genus Gillisia, Gillisia illustrilutea sp. nov. (type strain IC157T=ACAM 1062T=CIP 108530T), Gillisia sandarakina sp. nov. (type strain IC148T=ACAM 1060T=CIP 108529T) and Gillisia hiemivivida sp. nov. (type strain IC154T=ACAM 1061T=CIP 108528T).

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