Effects of Melissa officinalis L. Essential Oil in Comparison with Anaesthetics on Gill Tissue Damage, liver metabolism and Immune Parameters in Sea Bass (Lateolabrax maculatus) during Simulated live Transport

In the current study, Melissa officinalis L. essential oil (MOEO), a novel sedative and anaesthetic, was employed in transport water to obtain a lower stress effect and higher survival rate for live marine fish. The effect of MOEO and various types of anaesthetics, administered at a low temperature on gill morphology, liver function and immunological parameters of living sea bass (Lateolabrax maculatus) subjected to transport stress, was evaluated to optimize the anaesthetic and sedative concentrations during live sea bass transport. Light microscopy and scanning electron microscopy of sea bass, subjected to simulated live transport for 72 h, demonstrated that the changes in the morphological characteristics of gill tissue treated with 40 mg/L MOEO (A3 group) were minimal in comparison to those observed in untreated sea bass. The results of pyruvate kinase (PK), phosphofructokinase (PFK), hexokinase (HK), hepatic glycogen (Gly), superoxide dismutase (SOD), lipid peroxides (MDA) and Caspase-3 assays indicated that the glycolysis rate, energy consumption, lipid peroxidation and hepatocyte apoptosis were the lowest in the A3 group. The values of the two immune parameters, lysozyme (LZM) and fish immunoglobulin M (IgM), indicated the strongest immunity ability in the A3 group. After 12 h recovery, sea bass treated with 30 mg/L MS-222 (B group) displayed a 100% survival rate, sea bass treated with 20 mg/L (A2 group) and 40 mg/L (A3 group) MOEO displayed a 96% survival rate, sea bass treated with 20 mg/L eugenol (C group) had a 94% survival rate, and untreated sea bass (CK group) had a 50% survival rate. Therefore, the addition MOEO to the transport water had anaesthetic and sedative effects similar to MS-222 and eugenol. The results confirmed that the addition of MOEO to the transport water could reduce tissue damage, energy metabolism, and the oxidative stress response in sea bass during transport.

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Melissa officinalis essential oil loaded glycerosomes: preparation and in vitro activity evaluation against herpes labialis (HSV-1)

10.1055/s-0039-3399654 ◽

2019 ◽

Author(s):

G Vanti ◽

V Dourdouni ◽

D Lazari ◽

C Panagiotidis ◽

SG Ntallis ◽

...

Keyword(s):

Essential Oil ◽

Vitro Activity ◽

Herpes Labialis ◽

In Vitro Activity ◽

Melissa Officinalis ◽

Activity Evaluation ◽

Hsv 1

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Effect of particle size of perlite and its mixture with peat moss on essential oil percent and yield of lemon balm (Melissa officinalis) in hydroponic system

Journal of Science and Technology of Greenhouse Culture ◽

10.29252/ejgcst.9.3.39 ◽

2018 ◽

Vol 9 (3) ◽

pp. 39-48

Author(s):

E. Farrokhi ◽

A. Samadi ◽

A. Rahimi ◽

F. Asadzadeh ◽

◽

...

Keyword(s):

Particle Size ◽

Essential Oil ◽

Peat Moss ◽

Melissa Officinalis ◽

Hydroponic System ◽

Lemon Balm ◽

Effect Of Particle Size

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Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽

10.3390/antiox10071081 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1081

Author(s):

Matilda Rădulescu ◽

Călin Jianu ◽

Alexandra Teodora Lukinich-Gruia ◽

Marius Mioc ◽

Alexandra Mioc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Melissa Officinalis ◽

Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

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Systematic identification and expression analysis of the Sox gene family in spotted sea bass (Lateolabrax maculatus)

Comparative Biochemistry and Physiology Part D Genomics and Proteomics ◽

10.1016/j.cbd.2021.100817 ◽

2021 ◽

Vol 38 ◽

pp. 100817

Author(s):

Bingyu Li ◽

Yuan Tian ◽

Haishen Wen ◽

Xin Qi ◽

Lingyu Wang ◽

...

Keyword(s):

Gene Family ◽

Expression Analysis ◽

Sea Bass ◽

Lateolabrax Maculatus ◽

Sox Gene ◽

Systematic Identification

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Minerals, Essential Oils, and Biological Properties of Melissa officinalis L.

Plants ◽

10.3390/plants10061066 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1066

Author(s):

Fahima Abdellatif ◽

Muhammad Akram ◽

Samir Begaa ◽

Mohammed Messaoudi ◽

Adel Benarfa ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Antimicrobial Activities ◽

Biological Properties ◽

Phytopathogenic Fungi ◽

Melissa Officinalis ◽

Flame Ionization ◽

Analysis Technique ◽

Total Oil

This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.

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