Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection

Heterocyclic amine histamine is a well-known foodborne toxicant (mostly linked to “scombroid poisoning”) synthesized from the microbial decarboxylation of amino acid histidine. In this work, we report the fabrication of a flexible screen-printed immunosensor based on a silver electrode coated with single-walled carbon nanotubes (SWCNTs) for the detection of histamine directly in fish samples. Biosensors were realized by first spray depositing SWCNTs on the working electrodes and by subsequently treating them with oxygen plasma to reduce the unwanted effects related to their hydrophobicity. Next, anti-histamine antibodies were directly immobilized on the treated SWCNTs. Histamine was detected using the typical reaction of histamine and histamine-labeled with horseradish peroxidase (HRP) competing to bind with anti-histamine antibodies. The developed immunosensor shows a wide linear detection range from 0.005 to 50 ng/mL for histamine samples, with a coefficient of determination as high as 98.05%. Average recoveries in fish samples were observed from 96.00% to 104.7%. The biosensor also shows good selectivity (less than 3% relative response for cadaverine, putrescine, and tyramine), reproducibility, mechanical and time stability, being a promising analytical tool for the analysis of histamine, as well as of other food hazards.

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Development of Flexible Dispense-Printed Electrochemical Immunosensor for Aflatoxin M1 Detection in Milk

Sensors ◽

10.3390/s19183912 ◽

2019 ◽

Vol 19 (18) ◽

pp. 3912 ◽

Cited By ~ 5

Author(s):

Biresaw Demelash Abera ◽

Aniello Falco ◽

Pietro Ibba ◽

Giuseppe Cantarella ◽

Luisa Petti ◽

...

Keyword(s):

Limit Of Detection ◽

Low Cost ◽

Electrochemical Immunosensor ◽

Aflatoxin M1 ◽

Quality And Safety ◽

Buffer Solution ◽

Detection Range ◽

Fabrication Cost ◽

Food Quality And Safety ◽

Walled Carbon Nanotubes

Detection of mycotoxins, especially aflatoxin M1 (AFM1), in milk is crucial to be able to guarantee food quality and safety. In recent years, biosensors have been emerging as a fast, reliable and low-cost technique for the detection of this toxin. In this work, flexible biosensors were fabricated using dispense-printed electrodes, which were functionalized with single-walled carbon nanotubes (SWCNTs) and subsequently coated with specific antibodies to improve their sensitivity. Next, the immunosensor was tested for the detection of AFM1 in buffer solution and a spiked milk sample using a chronoamperometric technique. Results showed that the working range of the sensors was 0.01 µg/L at minimum and 1 µg/L at maximum in both buffer and spiked milk. The lower limit of detection of the SWCNT-functionalized sensor was 0.02 µg/L, which indicates an improved sensitivity compared to the sensors reported so far. The sensitivity and detection range were in accordance with the limitation values imposed by regulations on milk and its products. Therefore, considering the low fabrication cost, the ease of operation, and the rapid read-out, the use of this sensor could contribute to safeguarding consumers’ health.

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Photoluminescence-Based Bioassay With Cysteamine-Capped TiO2 Nanoparticles for the Selective Recognition of N-Acyl Homoserine Lactones

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.750933 ◽

2021 ◽

Vol 9 ◽

Author(s):

Sahana Vasudevan ◽

Parthasarathy Srinivasan ◽

Prasanna Neelakantan ◽

John Bosco Balaguru Rayappan ◽

Adline Princy Solomon

Keyword(s):

Financial Burden ◽

Intensity Variation ◽

Diagnostic Procedures ◽

Detection Range ◽

Homoserine Lactones ◽

Bacterial Signaling ◽

Acyl Homoserine Lactones ◽

Relative Response ◽

Linear Detection ◽

Microbial Infections

Currently available diagnostic procedures for infections are laborious and time-consuming, resulting in a substantial financial burden by increasing morbidity, increased costs of hospitalization, and mortality. Therefore, innovative approaches to design diagnostic biomarkers are imperative to assist in the rapid and sensitive diagnosis of microbial infections. Acyl homoserine lactones (AHLs) are ubiquitous bacterial signaling molecules that are found to be significantly upregulated in infected sites. In this pioneering work, we have developed a simple photoluminescence-based assay using cysteamine-capped titanium oxide (TiO2) nanoparticles for AHL detection. The PL intensity variation of the oxygen defect state of TiO2 was used for the biosensing measurements. The bioassays were validated using two well-studied AHL molecules (C4-HSL and 3-oxo-C12 HSL) of an important human pathogen, Pseudomonas aeruginosa. The developed system has a maximum relative response of 98%. Furthermore, the efficacy of the system in simulated host urine using an artificial urine medium showed a linear detection range of 10–160 nM. Also, we confirmed the relative response and specificity of the system in detecting AHLs produced by P. aeruginosa in a temporal manner.

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Multi-walled carbon nanotubes–chitosan with a branched structure modified with ferrocenecarboxylic acid for carcinoembryonic antigen detection

Analytical Methods ◽

10.1039/c5ay02030a ◽

2015 ◽

Vol 7 (23) ◽

pp. 10032-10039 ◽

Cited By ~ 6

Author(s):

Taotao Feng ◽

Xiuwen Qiao ◽

Haining Wang ◽

Zhao Sun ◽

Chenglin Hong

Keyword(s):

Carbon Nanotubes ◽

Carcinoembryonic Antigen ◽

Modified Electrode ◽

Electrochemical Immunosensor ◽

Antigen Detection ◽

Multi Walled Carbon Nanotubes ◽

Ferrocenecarboxylic Acid ◽

Branched Structure ◽

Walled Carbon Nanotubes

A novel electrochemical immunosensor for the detection of CEA was proposed based on Fc-COOH connected to the branched structure of a MWCNT–CS complex modified electrode.

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Label-Free Electrochemical Biosensor Based on Au@MoS₂–PANI for Escherichia coli Detection

Chemosensors ◽

10.3390/chemosensors9030049 ◽

2021 ◽

Vol 9 (3) ◽

pp. 49

Author(s):

Pushap Raj ◽

Man Hwan Oh ◽

Kyudong Han ◽

Tae Yoon Lee

Keyword(s):

Escherichia Coli ◽

Bacterial Infections ◽

Limit Of Detection ◽

Bacterial Pathogens ◽

Cost Effective ◽

Covalent Immobilization ◽

Label Free ◽

Self Assembled Monolayer ◽

Detection Range ◽

Linear Detection

Bacterial infections have become a significant challenge in terms of public health, the food industry, and the environment. Therefore, it is necessary to address these challenges by developing a rapid, cost-effective, and easy-to-use biosensor for early diagnosis of bacterial pathogens. Herein, we developed a simple, label-free, and highly sensitive immunosensor based on electrochemical detection using the Au@MoS₂–PANI nanocomposite. The conductivity of the glassy carbon electrode is greatly enhanced using the Au@MoS₂–PANI nanocomposite and a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface was employed for the covalent immobilization of antibodies to minimize the nonspecific adsorption of bacterial pathogens on the electrode surface. The biosensor established a high selectivity and sensitivity with a low limit of detection of 10 CFU/mL, and detected Escherichia coli within 30 min. Moreover, the developed biosensor demonstrated a good linear detection range, practical utility in urine samples, and electrode regenerative studies.

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Extended linear detection range for optical tweezers using a stop at the back focal plane of the condenser

Journal of Optics ◽

10.1088/2040-8978/17/6/065606 ◽

2015 ◽

Vol 17 (6) ◽

pp. 065606 ◽

Cited By ~ 3

Author(s):

S Masoumeh Mousavi ◽

Akbar Samadi ◽

Faegheh Hajizadeh ◽

S Nader S Reihani

Keyword(s):

Optical Tweezers ◽

Focal Plane ◽

Detection Range ◽

Linear Detection

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Flexible cotton-AuNP thread electrode for non-enzymatic sensor of uric acid in urine

10.21203/rs.3.rs-427183/v1 ◽

2021 ◽

Author(s):

Kanyapat Teekayupak ◽

Nipapan Ruecha ◽

Orawon Chailapakul ◽

Nadnudda Rodthongkum

Keyword(s):

Uric Acid ◽

Differential Pulse ◽

Detection Sensitivity ◽

Cotton Fibers ◽

Flexible Electrode ◽

Detection Range ◽

Sensor Platform ◽

Linear Detection ◽

Pulse Voltammetry ◽

Enzymatic Detection

Abstract We report on the development of an electrochemical sensor platform based on modified cotton fibers for the non-enzymatic detection of uric acid (UA), an important biomarker for gout disease. To create the flexible electrode, a cotton thread was coated with carbon ink followed by the electrodeposition of AuNPs. Then, differential pulse voltammetry (DPV) was used to evaluate the sensor performances, and a linear detection range between 10 µM and 5.0 mM of uric acid was obtained. The sensor has a low detection limit of 0.12 µM, which is optimal for use in the patients suffering from gout disease which commonly experience concentrations of uric acid in urine higher than 4.46 mM. Furthermore, we found that the detection sensitivity of the platform was not affected by the presence of other physiological compounds present in human urine. The described platform has the potential for integration in a diaper hence enabling rapid detection and screening for gout disease.

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Fluorescence Detection of Atmospheric SO2 Using Excitation by NO-γ-Bands

Zeitschrift für Naturforschung A ◽

10.1515/zna-1978-0704 ◽

1978 ◽

Vol 33 (7) ◽

pp. 782-785 ◽

Cited By ~ 1

Author(s):

F. Michels ◽

C. Zetzsch ◽

F. Stuhl

Keyword(s):

Fluorescence Detection ◽

Wavelength Region ◽

Time Response ◽

Detection Range ◽

Linear Detection

A method previously applied for the detection of NO was modified to also monitor SO2. With this method SO2 is excited by NO-γ-bands (A2 Σ+→X2 IIr) and is detected by its subsequent fluorescence in the wavelength region from 300 to 400 nm. The results indicate a linear detection range from 4 ppb to 100 ppm in air using a time response of 100 s. The interferences from some atmospheric constituents were investigated.

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Functionalization of single-walled carbon nanotubes by citric acid/oxygen plasma treatment

Fullerenes Nanotubes and Carbon Nanostructures ◽

10.1080/1536383x.2017.1347639 ◽

2017 ◽

Vol 25 (9) ◽

pp. 519-525 ◽

Cited By ~ 2

Author(s):

Mohammad Jellur Rahman ◽

Tetsu Mieno

Keyword(s):

Carbon Nanotubes ◽

Citric Acid ◽

Plasma Treatment ◽

Oxygen Plasma ◽

Single Walled Carbon Nanotubes ◽

Oxygen Plasma Treatment ◽

Single Walled Carbon ◽

Walled Carbon Nanotubes

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Rapid Quantitative Detection of Deltamethrin in Corydalis yanhusuo by SERS Coupled with Multi-Walled Carbon Nanotubes

Molecules ◽

10.3390/molecules25184081 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4081

Author(s):

Hui Zhang ◽

Pengcheng Nie ◽

Zhengyan Xia ◽

Xuping Feng ◽

Xiaoxi Liu ◽

...

Keyword(s):

Carbon Nanotubes ◽

Solid Phase ◽

Large Diameter ◽

Surface Enhanced Raman Spectroscopy ◽

Coefficient Of Determination ◽

Quantitative Detection ◽

Least Squares Regression ◽

Quechers Method ◽

Multi Walled Carbon Nanotubes ◽

Walled Carbon Nanotubes

With the increase in demand, artificially planting Chinese medicinal materials (CHMs) has also increased, and the ensuing pesticide residue problems have attracted more and more attention. An optimized quick, easy, cheap, effective, rugged and safe (QuEChERS) method with multi-walled carbon nanotubes as dispersive solid-phase extraction sorbents coupled with surface-enhanced Raman spectroscopy (SERS) was first proposed for the detection of deltamethrin in complex matrix Corydalis yanhusuo. Our results demonstrate that using the optimized QuEChERS method could effectively extract the analyte and reduce background interference from Corydalis. Facile synthesized gold nanoparticles with a large diameter of 75 nm had a strong SERS enhancement for deltamethrin determination. The best prediction model was established with partial least squares regression of the SERS spectra ranges of 545~573 cm−1 and 987~1011 cm−1 with a coefficient of determination (R2) of 0.9306, a detection limit of 0.484 mg/L and a residual predictive deviation of 3.046. In summary, this article provides a new rapid and effective method for the detection of pesticide residues in CHMs.

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