scholarly journals Genome-Wide Identification and Expression Analysis of SOS Response Genes in Salmonella enterica Serovar Typhimurium

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 943
Author(s):  
Angela Mérida-Floriano ◽  
Will P. M. Rowe ◽  
Josep Casadesús
Keyword(s):  
Dna Damage ◽  
Stress Responses ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Single Cell Analysis ◽  
Expression Patterns ◽  
Sos Response ◽  
Sos Induction ◽  
Serovar Typhimurium ◽  
Major Factors

A bioinformatic search for LexA boxes, combined with transcriptomic detection of loci responsive to DNA damage, identified 48 members of the SOS regulon in the genome of Salmonella enterica serovar Typhimurium. Single cell analysis using fluorescent fusions revealed that heterogeneous expression is a common trait of SOS response genes, with formation of SOSOFF and SOSON subpopulations. Phenotypic cell variants formed in the absence of external DNA damage show gene expression patterns that are mainly determined by the position and the heterology index of the LexA box. SOS induction upon DNA damage produces SOSOFF and SOSON subpopulations that contain live and dead cells. The nature and concentration of the DNA damaging agent and the time of exposure are major factors that influence the population structure upon SOS induction. An analogy can thus be drawn between the SOS response and other bacterial stress responses that produce phenotypic cell variants.

scholarly journals No Genetic Barriers between Salmonella enterica Serovar Typhimurium and Escherichia coli in SOS-Induced Mismatch Repair-Deficient Cells

2000 ◽  
Vol 182 (20) ◽  
pp. 5922-5924 ◽  
Author(s):  
Ivan Matic ◽  
François Taddei ◽  
Miroslav Radman
Keyword(s):  
Escherichia Coli ◽  
Mismatch Repair ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Sos Response ◽  
Sos Induction ◽  
Serovar Typhimurium ◽  
Genetic Barriers

ABSTRACT Conjugational crosses trigger SOS induction in Escherichia coli F− cells mated with Salmonella enterica serovar Typhimurium Hfr donors. Using an epigenetic indicator of SOS induction, we showed that a strong SOS response occurring in a subpopulation of mated mismatch repair-deficient cells totally abolishes genetic barriers between these two genera.

scholarly journals A Salmonella enterica Serovar Typhimurium hemA Mutant Is Highly Susceptible to Oxidative DNA Damage

2002 ◽  
Vol 184 (14) ◽  
pp. 3774-3784 ◽  
Author(s):  
Maya Elgrably-Weiss ◽  
Sunny Park ◽  
Eliana Schlosser-Silverman ◽  
Ilan Rosenshine ◽  
James Imlay ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Salmonella Enterica ◽  
Fenton Reaction ◽  
Oxidative Dna Damage ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Intracellular Iron ◽  
Serovar Typhimurium

ABSTRACT The first committed step in the biosynthesis of heme, an important cofactor of two catalases and a number of cytochromes, is catalyzed by the hemA gene product. Salmonella enterica serovar Typhimurium hemA26::Tn10d (hemA26) was identified in a genetic screen of insertion mutants that were sensitive to hydrogen peroxide. Here we show that the hemA26 mutant respires at half the rate of wild-type cells and is highly susceptible to the effects of oxygen species. Exposure of the hemA26 strain to hydrogen peroxide results in extensive DNA damage and cell death. The chelation of intracellular free iron fully abrogates the sensitivity of this mutant, indicating that the DNA damage results from the iron-catalyzed formation of hydroxyl radicals. The inactivation of heme synthesis does not change the amount of intracellular iron, but by diminishing the rate of respiration, it apparently increases the amount of reducing equivalents available to drive the Fenton reaction. We also report that hydrogen peroxide has opposite effects on the expression of hemA and hemH, the first and last genes of heme biosynthesis pathway, respectively. hemA mRNA levels decrease, while the transcription of hemH is induced by hydrogen peroxide, in an oxyR-dependent manner. The oxyR-dependent induction is suppressed under conditions that accelerate the Fenton reaction by a mechanism that is not yet understood.

Comparison of heat stress responses of immobilized and planktonic Salmonella enterica serovar Typhimurium

2013 ◽  
Vol 33 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Maj-Britt Nielsen ◽  
Gitte M. Knudsen ◽  
Vittoria Danino-Appleton ◽  
John E. Olsen ◽  
Line E. Thomsen
Keyword(s):  
Heat Stress ◽  
Stress Responses ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Serovar Typhimurium

scholarly journals Reporters for Single-Cell Analysis of Colicin Ib Expression in Salmonella enterica Serovar Typhimurium

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144647 ◽  
Author(s):  
Stefanie Spriewald ◽  
Jana Glaser ◽  
Markus Beutler ◽  
Martin B. Koeppel ◽  
Bärbel Stecher
Keyword(s):  
Single Cell ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Serovar Typhimurium

scholarly journals The Base Excision Repair System of Salmonella enterica serovar Typhimurium Counteracts DNA Damage by Host Nitric Oxide

2009 ◽  
Vol 5 (5) ◽  
pp. e1000451 ◽  
Author(s):  
Anthony R. Richardson ◽  
Khanh C. Soliven ◽  
Margaret E. Castor ◽  
Penelope D. Barnes ◽  
Stephen J. Libby ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Damage ◽  
Base Excision Repair ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Excision Repair ◽  
Repair System ◽  
Serovar Typhimurium ◽  
Base Excision ◽  
Base Excision Repair System

scholarly journals The GATC-Binding Protein SeqA Is Required for Bile Resistance and Virulence in Salmonella enterica Serovar Typhimurium

2007 ◽  
Vol 189 (23) ◽  
pp. 8496-8502 ◽  
Author(s):  
Ana I. Prieto ◽  
Marcello Jakomin ◽  
Ignacio Segura ◽  
M. Graciela Pucciarelli ◽  
Francisco Ramos-Morales ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Sos Response ◽  
Oral Route ◽  
Filament Formation ◽  
E Coli ◽  
Serovar Typhimurium ◽  
Bile Resistance ◽  
Increased Sensitivity ◽  
Response Envelope

ABSTRACT Disruption of the seqA gene of Salmonella enterica serovar Typhimurium causes defects similar to those described in E. coli: filament formation, aberrant nucleoid segregation, induction of the SOS response, envelope instability, and increased sensitivity to membrane-damaging agents. Differences between SeqA− mutants of E. coli and S. enterica, however, are found. SeqA− mutants of S. enterica form normal colonies and do not exhibit alterations in phage plaquing morphology. Lack of SeqA causes attenuation of S. enterica virulence by the oral route but not by the intraperitoneal route, suggesting a virulence defect in the intestinal stage of infection. However, SeqA− mutants are fully proficient in the invasion of epithelial cells. We hypothesize that attenuation of SeqA− mutants by the oral route may be caused by bile sensitivity, which in turn may be a consequence of envelope instability.

scholarly journals Uncovering a Large Set of Genes That Affect Surface Motility in Salmonella enterica Serovar Typhimurium

2006 ◽  
Vol 188 (22) ◽  
pp. 7981-7984 ◽  
Author(s):  
Qingfeng Wang ◽  
Susana Mariconda ◽  
Asaka Suzuki ◽  
Michael McClelland ◽  
Rasika M. Harshey
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Large Set ◽  
Flagellar Gene ◽  
Swarming Motility ◽  
Microarray Experiments ◽  
Serovar Typhimurium ◽  
Surface Motility

ABSTRACT We describe a large set of genes affecting motility in Salmonella enterica serovar Typhimurium. Identified in microarray experiments as displaying flagellar gene expression patterns or controlled by known flagellar regulators, we show that null mutations in these genes primarily affect swarming motility. Three genes function in chemotaxis.

Sign in / Sign up

Export Citation Format

Share Document