scholarly journals Electrochemical Studies on CaP Electrodeposition on Three Dimensional Surfaces of Selective Laser Melted Titanium Scaffold

Coatings ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 667 ◽  
Author(s):  
Xuetong Sun ◽  
Huaishu Lin ◽  
Chunyu Zhang ◽  
Jian Jin ◽  
Si Di
Keyword(s):  
Electrochemical Impedance ◽  
Three Dimensional ◽  
Basic Research ◽  
Electrochemical Process ◽  
Nucleation Behavior ◽  
Instantaneous Nucleation ◽  
Coating Morphology ◽  
Continuous Nucleation ◽  
Observation Results

In this work, calcium phosphate (CaP) coating was electrodeposited on the three dimensional surface of SLM-Ti scaffolds. The in situ measurement showed that the potential variation within 5 mm thickness porous selective laser melting (SLM)-Ti samples was about 80 mV as a result of the low conductivity of CaP coatings. SEM observation results revealed that the coating morphology depended on the distance between the surface position of porous SLM-Ti electrode and the auxiliary electrode. Based on the compared electrochemical experiments, it was found that the top and the bottom surfaces of SLM-Ti scaffolds exhibited continuous nucleation and instantaneous nucleation behavior respectively. The Electrochemical impedance spectroscopy (EIS) results also revealed that the electrodeposition processes at different depth of SLM-Ti scaffolds were not synchronized. These differences were ultimately caused by the non-uniform distribution of the potential and the current inside porous SLM-Ti electrodes. The present work provides a basic research method for studying the mechanism of the electrochemical process on three dimensional surfaces of SLM-Ti scaffolds.

scholarly journals In-situ multi-analytical study of ongoing corrosion processes on bronze artworks exposed outdoor

ACTA IMEKO ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 241
Author(s):  
Leila Es Sebar ◽  
Leonardo Iannucci ◽  
Caterina Gori ◽  
Alessandro Re ◽  
Marco Parvis ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Three Dimensional ◽  
Corrosion Products ◽  
Invasive Approach ◽  
Non Invasive ◽  
Combined Use ◽  
Art Collection ◽  
The Stability ◽  
Microclimate Conditions

<p class="Abstract">This paper presents a long-term in-situ campaign to monitor contemporary bronze statuary exposed outdoors. The case study relates to the characterisation of three sculptures belonging to the Gori Art Collection, located in the Fattoria di Celle: ‘Cavaliere’ and ‘Miracolo – Composizione’ by Marino Marini and ‘Due forme o due ombre n°2’ by Luciano Minguzzi. The overall conservation state of the sculptures was investigated by means of a multi-analytical and non-invasive approach, involving different techniques. Three-dimensional photogrammetry was performed to fully document the artworks. The chemical and microstructural features of the corrosion patinas were then characterised through X-ray fluorescence and Raman spectroscopy. In addition, the stability and the protective effectiveness of the corrosion products were assessed by electrochemical impedance spectroscopy. Thanks to the combined use of these specific techniques, the information extracted through the different analyses could be correlated with each other and with the exposure conditions. The different corrosion products were identified as being primarily copper sulphates and phosphates, and they were correlated with the different microclimate conditions related to their location on the statues. The information gathered from the presented multi-analytical approach represents the fundamental knowledge required to develop a tailored conservation project to assure the long-lasting preservation of these artworks.</p>

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

In situ investigation of the primary recrystallization of alpha titanium in HVEM

1978 ◽  
Vol 36 (1) ◽  
pp. 634-635
Author(s):  
S. Naka ◽  
R. Penelle ◽  
R. Valle
Keyword(s):  
Dimensional Analysis ◽  
Texture Evolution ◽  
Cold Work ◽  
Three Dimensional ◽  
Primary Recrystallization ◽  
Thin Foils ◽  
In Situ Investigation ◽  
Grain Growth Model ◽  
Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

Detection and mapping of interactions between chromatin and the nuclear envelope in drosophila embryos

1995 ◽  
Vol 53 ◽  
pp. 764-765
Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat
Keyword(s):  
Nuclear Envelope ◽  
Chromatin Condensation ◽  
Three Dimensional ◽  
Physiological Role ◽  
Nuclear Surface ◽  
Intact Cells ◽  
Molecular Determinants ◽  
Surface Harmonic ◽  
Drosophila Embryos

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

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