scholarly journals Encapsulated Activated Grape Seed Extract: A Novel Formulation with Anti-Aging, Skin-Brightening, and Hydration Properties

Cosmetics ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 4
Author(s):  
Kan Tao ◽  
Lili Guo ◽  
Xincheng Hu ◽  
Corey Fitzgerald ◽  
Karl Rouzard ◽  
...  

Protein phosphatase 2A (PP2A) is a master regulatory protein that plays a critical role in oxidative stress signaling. A novel, proprietary grape seed extract called Activated Grape Seed Extract (AGSE), enriched for PP2A-activating flavonoids, was recently developed and demonstrated to have antioxidant and anti-inflammatory activities. AGSE is a purple-colored powder, with limited solubility restricting its use in a broad range of formulations. Our aim was to develop a formulation that reduced the color and increased the solubility of AGSE, allowing its skin-health-enhancing properties to be utilized in a wider array of products, and to test it clinically. Encapsulation was performed utilizing a liposome and hydroxypropyl-β-cyclodextrin, (HPCD)-based approach to produce Encapsulated AGSE (E-AGSE). Human dermal fibroblasts and epidermal keratinocytes were used to determine expression levels of aging and dermal–epidermal junction (DEJ) markers. EpiDerm™ was UVB-irradiated to measure the effects against cytokine release, DNA damage, apoptosis, and skin barrier. Human melanocytes were used to determine melanin production and mushroom tyrosinase was used for inhibitory activity. A 4-week, 31-subject sensitive-skin clinical was performed with 2% E-AGSE Essence to assess its activity on human skin. We demonstrated that E-AGSE inhibits PP2A demethylation, increases key anti-aging (collagen I, III, elastin) and DEJ markers, protects against UVB-induced DNA damage, reduces inflammation, and promotes filaggrin in vitro. Moreover, E-AGSE reduces melanin production via tyrosinase inhibition. Clinical assessment of E-AGSE showed that it reduces the appearance of wrinkles, brightens the skin, and boosts hydration. E-AGSE is a novel grape seed extract formulation enriched for PP2A-activating flavonoids that is clinically effective in sensitive skin, providing several benefits.

2015 ◽  
Vol 3 (7) ◽  
pp. 392-402
Author(s):  
Maurizio Fracchiolla ◽  
Mark McKenna ◽  
Kaisa Zaman ◽  
Patrick Becker ◽  
Thomas Terraz

2006 ◽  
Vol 68 (6) ◽  
pp. 469-473 ◽  
Author(s):  
Muthaiya Balu ◽  
Purushotham Sangeetha ◽  
Ganesan Murali ◽  
Chinnakannu Panneerselvam

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6351
Author(s):  
Kristen L. Huber ◽  
José R. Fernández ◽  
Corey Webb ◽  
Karl Rouzard ◽  
Jason Healy ◽  
...  

Environmental stimuli attack the skin daily resulting in the generation of reactive oxygen species (ROS) and inflammation. One pathway that regulates oxidative stress in skin involves Protein Phosphatase 2A (PP2A), a phosphatase which has been previously linked to Alzheimer’s Disease and aging. Oxidative stress decreases PP2A methylation in normal human dermal fibroblasts (NHDFs). Thus, we hypothesize agents that increase PP2A methylation and activity will promote skin health and combat aging. To discover novel inhibitors of PP2A demethylation activity, we screened a library of 32 natural botanical extracts. We discovered Grape Seed Extract (GSE), which has previously been reported to have several benefits for skin, to be the most potent PP2A demethylating extract. Via several fractionation and extraction steps we developed a novel grape seed extract called Activated Grape Seed Extract (AGSE), which is enriched for PP2A activating flavonoids that increase potency in preventing PP2A demethylation when compared to commercial GSE. We then determined that 1% AGSE and 1% commercial GSE exhibit distinct gene expression profiles when topically applied to a 3D human skin model. To begin to characterize AGSE’s activity, we investigated its antioxidant potential and demonstrate it reduces ROS levels in NHDFs and cell-free assays equal to or better than Vitamin C and E. Moreover, AGSE shows anti-inflammatory properties, dose-dependently inhibiting UVA, UVB and chemical-induced inflammation. These results demonstrate AGSE is a novel, multi-functional extract that modulates methylation levels of PP2A and supports the hypothesis of PP2A as a master regulator for oxidative stress signaling and aging in skin.


2013 ◽  
Vol 66 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Hanaa A. Hassan ◽  
Gamal M. Edrees ◽  
Ezz M. El-Gamel ◽  
Elsamra A. El-sayed

Author(s):  
Mabrouk Attia Abd Eldaim ◽  
Ehab Tousson ◽  
Mohamed Mohamed Soliman ◽  
Ibrahim El Tantawy El Sayed ◽  
Abdel Aleem H. Abdel Aleem ◽  
...  

2009 ◽  
Author(s):  
Tiberius Dicu ◽  
Ion D. Postescu ◽  
Vasile Foriş ◽  
Ioana Brie ◽  
Eva Fischer-Fodor ◽  
...  

Author(s):  
Souad El Gengaihi ◽  
Doha H. Abou Baker

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.


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