Neuroprotective effects of grape seed extract on neuronal injury by inhibiting DNA damage in the gerbil hippocampus after transient forebrain ischemia

Background: The risk of occupational exposure to acrylamide is high and long-term acrylamide exposure can cause neurotoxicity. Thus, therapeutic agents that can protect against acrylamide-induced neurotoxicity are needed. Objective: To investigate whether Grape Seed Extract (GSE) protects against acrylamide-induced neurotoxicity in mice. Methods: Mice were divided into saline, GSE, acrylamide, GSE followed by acrylamide, acrylamide followed by GSE, and simultaneous acrylamide and GSE treatment groups. Gene expression and antioxidant enzyme levels were then determined using RT-PCR and biochemical assays. Results: Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) significantly upregulated in GSE-treated mice, compared to those in untreated controls. In contrast, Gpx1 (P < 0.05), Prdx3 (P < 0.05), SOD1 (P < 0.05), and CAT (P < 0.05) significantly downregulated in acrylamide-treated mice compared to those in untreated controls. Results of the treatment with GSE before exposure to acrylamide or simultaneously with acrylamide indicated that GSE restored Gpx1, Prdx3, SOD1, and CAT expression to similar levels as those in the control group. GSE treatment after exposure to acrylamide did not exert any neuroprotective effects against acrylamide, as revealed by significant downregulation of Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) compared to that in untreated controls. Animals treated with grape seed before acrylamide treatment showed no significant change in LPO activities and a significant increase in GSH levels, compared to those in untreated controls. Conclusion: GSE exerted neuroprotective effects against acrylamide-induced neurotoxicity. Acrylamide caused oxidative stress 20 days post-exposure. However, grape seed treatment before exposure to acrylamide restored all test parameters to levels similar to control values.

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Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats

Brain Research Bulletin ◽

10.1016/j.brainresbull.2005.10.007 ◽

2006 ◽

Vol 68 (6) ◽

pp. 469-473 ◽

Cited By ~ 55

Author(s):

Muthaiya Balu ◽

Purushotham Sangeetha ◽

Ganesan Murali ◽

Chinnakannu Panneerselvam

Keyword(s):

Central Nervous System ◽

Dna Damage ◽

Nervous System ◽

Oxidative Dna Damage ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Age Related

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Amelioration of cisplatin-induced nephrotoxicity by grape seed extract and fish oil is mediated by lowering oxidative stress and DNA damage

Cytotechnology ◽

10.1007/s10616-013-9589-8 ◽

2013 ◽

Vol 66 (3) ◽

pp. 419-429 ◽

Cited By ~ 29

Author(s):

Hanaa A. Hassan ◽

Gamal M. Edrees ◽

Ezz M. El-Gamel ◽

Elsamra A. El-sayed

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Fish Oil ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract

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Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice

Environmental Science and Pollution Research ◽

10.1007/s11356-021-13904-8 ◽

2021 ◽

Author(s):

Mabrouk Attia Abd Eldaim ◽

Ehab Tousson ◽

Mohamed Mohamed Soliman ◽

Ibrahim El Tantawy El Sayed ◽

Abdel Aleem H. Abdel Aleem ◽

...

Keyword(s):

Dna Damage ◽

Protein Expression ◽

Solid Tumor ◽

P53 Protein ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Hepatic Tissue ◽

P53 Protein Expression

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Neuroprotective effects of Buyang Huanwu Decoction on neuronal injury in hippocampus after transient forebrain ischemia in rats

Neuroscience Letters ◽

10.1016/s0304-3940(03)00522-6 ◽

2003 ◽

Vol 346 (1-2) ◽

pp. 29-32 ◽

Cited By ~ 43

Author(s):

Xiao-Ming Li ◽

Xiao-Chun Bai ◽

Lu-Ning Qin ◽

Hao Huang ◽

Zhong-Ju Xiao ◽

...

Keyword(s):

Neuronal Injury ◽

Forebrain Ischemia ◽

Neuroprotective Effects ◽

Buyang Huanwu Decoction ◽

Transient Forebrain Ischemia

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Encapsulated Activated Grape Seed Extract: A Novel Formulation with Anti-Aging, Skin-Brightening, and Hydration Properties

Cosmetics ◽

10.3390/cosmetics9010004 ◽

2021 ◽

Vol 9 (1) ◽

pp. 4

Author(s):

Kan Tao ◽

Lili Guo ◽

Xincheng Hu ◽

Corey Fitzgerald ◽

Karl Rouzard ◽

...

Keyword(s):

Dna Damage ◽

Critical Role ◽

Regulatory Protein ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Dermal Fibroblasts ◽

Epidermal Keratinocytes ◽

Sensitive Skin ◽

Melanin Production

Protein phosphatase 2A (PP2A) is a master regulatory protein that plays a critical role in oxidative stress signaling. A novel, proprietary grape seed extract called Activated Grape Seed Extract (AGSE), enriched for PP2A-activating flavonoids, was recently developed and demonstrated to have antioxidant and anti-inflammatory activities. AGSE is a purple-colored powder, with limited solubility restricting its use in a broad range of formulations. Our aim was to develop a formulation that reduced the color and increased the solubility of AGSE, allowing its skin-health-enhancing properties to be utilized in a wider array of products, and to test it clinically. Encapsulation was performed utilizing a liposome and hydroxypropyl-β-cyclodextrin, (HPCD)-based approach to produce Encapsulated AGSE (E-AGSE). Human dermal fibroblasts and epidermal keratinocytes were used to determine expression levels of aging and dermal–epidermal junction (DEJ) markers. EpiDerm™ was UVB-irradiated to measure the effects against cytokine release, DNA damage, apoptosis, and skin barrier. Human melanocytes were used to determine melanin production and mushroom tyrosinase was used for inhibitory activity. A 4-week, 31-subject sensitive-skin clinical was performed with 2% E-AGSE Essence to assess its activity on human skin. We demonstrated that E-AGSE inhibits PP2A demethylation, increases key anti-aging (collagen I, III, elastin) and DEJ markers, protects against UVB-induced DNA damage, reduces inflammation, and promotes filaggrin in vitro. Moreover, E-AGSE reduces melanin production via tyrosinase inhibition. Clinical assessment of E-AGSE showed that it reduces the appearance of wrinkles, brightens the skin, and boosts hydration. E-AGSE is a novel grape seed extract formulation enriched for PP2A-activating flavonoids that is clinically effective in sensitive skin, providing several benefits.

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The Effect of a Grape Seed Extract on Radiation-Induced DNA Damage in Human Lymphocytes

10.1063/1.3153444 ◽

2009 ◽

Author(s):

Tiberius Dicu ◽

Ion D. Postescu ◽

Vasile Foriş ◽

Ioana Brie ◽

Eva Fischer-Fodor ◽

...

Keyword(s):

Dna Damage ◽

Human Lymphocytes ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Radiation Induced

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Grape Seeds Extract as Brain Food: A Review

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i1.8270 ◽

2017 ◽

Vol 9 (1) ◽

Author(s):

Souad El Gengaihi ◽

Doha H. Abou Baker

Keyword(s):

Antioxidant Activities ◽

Complex Mixture ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Amyloid Peptide ◽

Grape Seeds ◽

Potential Benefits ◽

Reduced Risk

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.

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