scholarly journals Ultrasound-Assisted Extraction as a First Step in a Biorefinery Strategy for Valorisation of Extracted Olive Pomace

Energies ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2679 ◽  
Author(s):  
José Carlos Martínez-Patiño ◽  
Irene Gómez-Cruz ◽  
Inmaculada Romero ◽  
Beatriz Gullón ◽  
Encarnación Ruiz ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Total Phenolic ◽  
Natural Food ◽  
Antioxidant Compounds ◽  
Olive Pomace ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Mediterranean Countries ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Currently, interest in finding new feedstock as sources of natural food antioxidants is growing. The extracted olive pomace (EOP), which is an agro-industrial residue from the olive pomace extracting industries, is generated yearly in big amounts, mainly in the Mediterranean countries. EOP was subjected to an ultrasound assisted extraction with ethanol-water mixtures. The effect of main parameters, such as ethanol concentration (30–70% v/v), ultrasound amplitude (20–80%), and extraction time (5–15 min), on the extraction of antioxidant compounds was evaluated according to a Box–Behnken experimental design. The antioxidant capacity of the resulting extracts was determined by measuring their content in total phenolic compounds (TPC) and flavonoids (TFC), as well as their antioxidant activity by DPPH, ferric reducing antioxidant power (FRAP), and ABTS assays. Considering the simultaneous maximization of these five responses, the optimal conditions were found to be 43.2% ethanol concentration, 70% amplitude, and 15 min. The ultrasound assisted extraction of EOP under these optimized conditions yielded an extract with a phenolic and flavonoid content (per gram of EOP) of 57.5 mg gallic acid equivalent (GAE) and 126.9 mg rutin equivalent (RE), respectively. Likewise, the values for DPPH, ABTS, and FRAP assay (per gram of EOP) of 56.7, 139.1, and 64.9 mg Trolox equivalent, respectively were determined in the optimized extract.

scholarly journals Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 267
Author(s):  
Fátima Lameirão ◽  
Diana Pinto ◽  
Elsa F. Vieira ◽  
Andreia F. Peixoto ◽  
Cristina Freire ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Raw Material ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 °C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 µg/mg dw), followed by caffeic acid derivative (15.4 µg/mg dw) and epigallocatechin (15.3 µg/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NO● (IC50 = 0.1 µg/mL) and HOCl (IC50 = 0.7 µg/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 μg/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.

scholarly journals Ultrasound-Assisted Extraction of Total Phenolic Compounds fromInula helenium

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Jin Wang ◽  
Yong-Ming Zhao ◽  
Ya-Ting Tian ◽  
Chun-Lin Yan ◽  
Chun-Yan Guo
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Liquid Ratio ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasound Assisted ◽  
Solid Liquid

Ultrasound-assisted extraction (UAE) of phenolic compounds fromInula heleniumwas studied. Effects of ethanol concentration, ultrasonic time, solid-liquid ratio, and number of extractions were investigated. An orthogonal array was constructed to optimize UAE process. The optimized extraction conditions were as follows: ethanol concentration, 30%; solid-liquid ratio, 1 : 20; number of extractions, 2 times; extraction time, 30 min. Under the optimal conditions, the yield of total phenolic compounds and chlorogenic acid was6.13±0.58and1.32±0.17 mg/g, respectively. The results showed that high amounts of phenolic compounds can be extracted fromI. heleniumby ultrasound-assisted extraction technology.

scholarly journals Ultrasound-Assisted Extraction of Bioactive Compounds and Antioxidant Capacity for the Valorization of Elaeocarpus serratus L. Leaves

Processes ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 1218
Author(s):  
Ying-Hsuan Chen ◽  
Chun-Yao Yang
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Order Kinetic ◽  
Ultrasound Assisted Extraction ◽  
Sulfate Heptahydrate ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Elaeocarpus serratus L. leaves contain significant polyphenols, flavonoids, and myricitrin with medicinal property, which are byproducts after harvest and can be recovered for potential food applications. In this study, the effective extraction method for bioactive compounds and myricitrin from Elaeocarpus serratus L. leaves was investigated by comparing ultrasound-assisted extraction (UAE) and shaking-assisted extraction (SAE) for total phenolic content (TPC), total flavonoid content (TFC), myricitrin, and antioxidant capacity (as ferric reducing antioxidant power, FRAP). A first-order kinetic model of extraction was also proposed to correlate the observed extraction rate coefficient and limiting concentration of myricitrin in various solvents. Using defatted leaves (DL) as the substrate at the UAE condition of 95% ethanol with 1/10 (g/mL) of DL to solvent ratio under 40 kHz/300 W of ultrasound, 26.63% of yield of extract, and 199.75 mg-ferrous-sulfate-heptahydrate-equivalent/g-DL of FRAP were obtained. Further, the bioactive compounds extracted were 92.35 mg-gallic-acid-equivalent/g-DL of TPC, 53.14 mg-rutin-equivalent/g-DL of TFC, and 4.41 mg/g-DL of myricitrin, which were 1.53, 1.44, and 1.4 folds using SAE at 120 rpm, respectively. This showed that UAE was more efficient than SAE in bioactive compounds recovery for valorization of Elaeocarpus serratus L. leaves.

scholarly journals Exhausted Olive Pomace Phenolic-Rich Extracts Obtention: A First Step for a Biorefinery Scheme Proposal

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 10
Author(s):  
Irene Gómez-Cruz ◽  
Inmaculada Romero ◽  
María del Mar Contreras ◽  
Carmen Padilla-Rascón ◽  
Florbela Carvalheiro ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Added Value ◽  
Total Phenolic ◽  
Olive Pomace ◽  
Residual Oil ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The exhausted olive pomace (EOP) is the main residue generated year-round after the extraction of the residual oil contained in the olive pomace. Due to its chemical composition, EOP is an interesting bioresource for the production of bioenergy and high added-value products. Therefore, the purpose of this study was to evaluate the extraction of antioxidants from EOP using sequential ultrasound-assisted extraction (UAE) and maceration with constant stirring. Water and 70% acetone were used as solvents. The total phenolic content was determined by the Folin–Ciocalteu method, the antioxidant capacity was investigated and the phenolic extracts were also characterized.

scholarly journals Optimization of Oleuropein and Luteolin-7-O-Glucoside Extraction from Olive Leaves by Ultrasound-Assisted Technology

Energies ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2486 ◽  
Author(s):  
Antonio Lama-Muñoz ◽  
María del Mar Contreras ◽  
Francisco Espínola ◽  
Manuel Moya ◽  
Inmaculada Romero ◽  
...  
Keyword(s):  
Large Scale ◽  
Soxhlet Extraction ◽  
Operating Conditions ◽  
Antioxidant Compounds ◽  
Olive Leaves ◽  
Ultrasound Assisted Extraction ◽  
Mediterranean Countries ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
High Concentrations

The olive orchard cultivation in Mediterranean countries results in huge amounts of lignocellulosic biomass residues. One of the main residues are olive leaves. Olive leaves contain high concentrations of bioactive antioxidant compounds like oleuropein and luteolin-7-glucoside. The production of biactive compounds from olive leaves requires treatments capable of breaking the lignocellulosic structure. Current research focuses on use of inexpensive, quick, and not harmful to the environment treatments, searching a more simplified large-scale operation approach. Recently, advances in applied chemistry have led to possible new emerging industrial techniques like ultrasound-assisted extraction (UAE). This technology is a promising candidate as a green treatment solution for olive leaves utilization in a biorefinery. However, this application goes through prior optimization of technique and operating conditions. The goal of this study was to optimize the extraction of oleuropein and luteolin-7-glucoside from olive leaves through an investigation of the influence of key factors of ultrasound-assisted extraction using an experimental central composite design, in comparison with conventional Soxhlet extraction. The highest extraction efficiency and antioxidant capacity were obtained under optimal increment of temperature and amplitude conditions (40 °C and 30%, respectively). Values for oleuropein, luteolin-7-glucoside were 69.91 g/kg and 1.82 g/kg, respectively.

scholarly journals Release of Antioxidant Compounds of Zingiber officinale by Ultrasound-Assisted Aqueous Extraction and Evaluation of Their In Vitro Bioaccessibility

2020 ◽  
Vol 10 (14) ◽  
pp. 4987
Author(s):  
Elizabeth Contreras-López ◽  
Araceli Castañeda-Ovando ◽  
Judith Jaimez-Ordaz ◽  
Nelly del Socorro Cruz-Cansino ◽  
Luis Guillermo González-Olivares ◽  
...  
Keyword(s):  
Aqueous Media ◽  
Antioxidant Properties ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
In Vitro Bioaccessibility ◽  
Ultrasound Assisted

Ginger rhizome is widely used in culinary preparations and in traditional medicine. Its benefits are associated with its antioxidant properties related to phenolics and terpenoids compounds, which use to be thermolabile. Ultrasound-assisted extraction has been useful for enhancing the release of thermosensitive compounds present in vegetable tissues. Therefore, the aim of this study was to evaluate the influence of ultrasound-assisted extraction on the release of antioxidants from ginger in aqueous media as well as their in vitro bioaccessibility. Central composite rotatable design was applied to obtain the optimal conditions for the extraction; the variables studied were amplitude (80–90%) and temperature (30–50 °C). Total phenolic content, antioxidant capacity (DPPH•, ABTS•+ and FRAP), and in vitro bioaccessibility were determined. Amplitude was the main parameter influencing the extraction of antioxidants. The ginger aqueous extracts showed a bioaccessibility of around 30%. The release of antioxidant compounds from ginger by ultrasound-assisted extraction avoids the use of high temperatures and solvents commonly used in conventional extraction methods.

scholarly journals Ultrasound-Assisted Extraction of Semi-Defatted Unripe Genipap (Genipa americana L.): Selective Conditions for the Recovery of Natural Colorants

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1435
Author(s):  
Grazielle Náthia-Neves ◽  
Ádina L. Santana ◽  
Juliane Viganó ◽  
Julian Martínez ◽  
Maria Angela A. Meireles
Keyword(s):  
Antioxidant Capacity ◽  
Wide Spectrum ◽  
Raw Material ◽  
Total Phenolic ◽  
Natural Food ◽  
Fast Method ◽  
Ultrasound Assisted Extraction ◽  
Reducing Ability ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Ultrasound-assisted extraction (UAE) of semi-defatted unripe genipap (SDG) using supercritical CO2 was performed to enhance the recovery of natural colorant iridoids genipin and geniposide. There are currently few natural sources of iridoids, and their application as colorants is scarce. The UAE resulted in extracts with blue and green colors using water and ethanol, respectively. The highest global yield and genipin content was recovered with water, and the geniposide was significantly recovered with ethanol. With water at 450 W, the UAE raised the maximum global yield (25.50 g/100 g raw material). At 150 W and 7 min, the maximum content of genipin (121.7 mg/g extract) and geniposide (312 mg/g extract) was recovered. The total phenolic content (TPC) and antioxidant capacity with the oxygen reactive antioxidant capacity (ORAC) assay were also high in aqueous extracts. Ethanolic extracts showed high ferric-reducing ability antioxidant potential (FRAP) values. UAE showed an efficient and fast method to obtain different extracts’ fractions from SDG, which have a wide spectrum of applications, especially as natural food colorants.

scholarly journals Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against Alzheimer’s disease

2020 ◽  
Vol 50 ◽  
Author(s):  
Hafiz Majid ◽  
Filipa V. M. Silva
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Ache Inhibition ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: Alzheimer’s disease is a progressive mental deterioration related to ageing and senility. Approved drugs that inhibit acetylcholinesterase (AChE) enzyme activity in the human brain are one of the ways to control the natural progression of this disease. The present study reports on the optimisation of ultrasound-assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka leaves using response surface methodology. Methods: A Box-Behnken design was used to investigate the effect of extraction temperature (40–60°C), time (1–20 min), and ethanol concentration (30–70%) on AChE inhibition, antioxidant activity, and extraction yield. Results: The values of AChE, radical scavenging activity (RSA) and yield predicted by the models generated were similar to the experimental values. Extraction time, ethanol concentration and temperature were significant in all the responses. Optimum extraction conditions for maximum AChE inhibition (74%), RSA (79%) and yield (50%) were successfully validated experimentally and the IC50 of the optimised extracts were reduced to 28.5 (from 66.0) and 2.37 (from 32.4) ?g/ mL for AChE and antioxidant activity, respectively. The optimisation enabled an increase in the extraction yield from 21% to 49%. Conclusions: In view of the significant bioactive properties determined, with possible beneficial effects on memory deficit, we would encourage the use of the manuka leaf extract for the development of new phytopharmaceuticals to improve brain function and control dementias such as Alzheimer’s disease. One other application could be as a beverage for the preparation of tea infusions.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolics from Sideritis raeseri Using Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3949
Author(s):  
Katarina Šavikin ◽  
Jelena Živković ◽  
Teodora Janković ◽  
Nada Ćujić-Nikolić ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study we define the optimal conditions for ultrasound-assisted extraction of bioactive polyphenols from S. raeseri aerial parts using response surface methodology. The influence of ethanol concentration (10–90%), extraction temperature (20–80 °C), extraction time (10–60 min), and solid-to-solvent ratio (1:10–1:50) on total phenolic content as well as on content of individual flavonoids, and hypolaetin and isoscutellarein derivatives was studied. For the experimental design, a central composite design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenol from 19.32 to 47.23 mg GAE/g dw, HYP from 1.05 to 11.46 mg/g dw, ISC 1 from 0.68 to 10.68 mg/g dw, and ISC 2 from 0.74 to 15.56 mg/g dw. The optimal extraction conditions were set as: ethanol concentration of 65%, extraction time of 50 min, extraction temperature of 63 °C, and solid-to-solvent ratio of 1:40. Contents of TP, HYP, ISC 1, and ISC 2 in optimal extracts were 47.11 mg GAE/g dw, 11.73 mg/g dw, 9.54 mg/g dw, and 15.40 mg/g dw, respectively. Experimentally set values were in good agreement with those predicted by the response surface methodology model, indicating suitability of the used model, as well as the success of response surface methodology in optimizing the conditions of the extraction.

scholarly journals Optimization of Ficus deltoidea Using Ultrasound-Assisted Extraction by Box-Behnken Statistical Design

2016 ◽  
Vol 02 ◽  
pp. 71 ◽  
Author(s):  
L. J. Ong ◽  
Awang Soh Mamat ◽  
Muhammad Shahzad Aslam ◽  
Muhammad Syarhabil Ahmad ◽  
◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Statistical Design ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Sonication Time ◽  
Ultrasound Assisted Extraction ◽  
Ficus Deltoidea ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Sample Ratio

In this study, the effect of extraction parameters (ethanol concentration, sonication time, and solvent-to-sample ratio) onFicus deltoidealeaves was investigated using ultrasound-assisted extraction by response surface methodology (RSM). Total phenolic content (TPC) ofF. deltoideaextracts was identified using Folin-Ciocalteu method and expressed in gallic acid equivalent (GAE) per g. Box-Behnken statistical design (BBD) was the tool used to find the optimal conditions for maximum TPC. Besides, the extraction yield was measured and stated in percentage. The optimized TPC attained was 455.78 mg GAE/g at 64% ethanol concentration, 10 minutes sonication time, and 20 mL/g solvent-to-sample ratio whereas the greatest extraction yield was 33% with ethanol concentration of 70%, sonication time of 40 minutes, and solvent-to-material ratio at 40 mL/g. The determination coefficient,R2, for TPC indicates that 99.5% capriciousness in the response could be clarified by the ANOVA model and the value of 0.9681 of predictedR2is in equitable agreement with the 0.9890 of adjustedR2. The present study shows that ethanol water as solvent, a short time of 10 minutes, and adequate solvent-to-sample ratio (20 mL/g) are the best conditions for extraction.

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