Periodontal Regeneration

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

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Microenvironment Influence of a Novel Bioengineered Wound Product, APIS®: A Preliminary In Vitro Analysis of Inflammatory Marker and Growth Factor Secretion

International Journal of Biomaterials ◽

10.1155/2021/6612870 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Isaac Rodriguez ◽

Tricia Conti ◽

Nina Bionda

Keyword(s):

Biological Activity ◽

Growth Factors ◽

Growth Factor ◽

Chronic Wounds ◽

Inflammatory Marker ◽

Protein Levels ◽

Vitro Analysis ◽

Related Proteins ◽

Endothelial Growth Factor

Objective. Preliminary biological activity assessment of a novel bioengineered wound product (APIS®, SweetBio, Inc., Memphis, TN, USA), a synthesis of gelatin, Manuka honey, and hydroxyapatite, with in vitro indications to protect, instill balance to, and progress the wound microenvironment. Approach. The biological activity the bioengineered wound product (BWP) elicits on human cells in vitro was assessed by evaluating matrix metalloproteinase- (MMP-) related proteins expressed by macrophages and secretion of growth factors in fibroblasts. Cells were cultured with no treatment, stimulated with lipopolysaccharides (LPS), or seeded directly on the BWP for 24 hours. An additional 72-hour time point for the BWP was assessed to determine if the BWP maintained its activity compared to itself at 24 hours. Cell culture supernatants were assayed to quantify secreted protein levels. Results. MMP-9 secretion from macrophages seeded on the BWP were nondetectable ( P < 0.01 ), while a tissue inhibitor of MMP (TIMP-1) was detected. This decreased the overall MMP-9/TIMP-1 ratio secreted from macrophages seeded on the BWP compared to the controls. Additionally, the secretion of prohealing growth factors such as basic fibroblast growth factor (FGFb) and vascular endothelial growth factor (VEGF) was observed. Conclusion. Results from this preliminary in vitro evaluation suggest that the BWP has the potential to instill balance to the wound microenvironment by reducing the MMP-9/TIMP-1 ratio secretion from macrophages and progress previously stalled chronic wounds towards healing by triggering the release of growth factors from fibroblasts.

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Identifying the Growth Factors for Improving Neointestinal Regeneration in Rats through Transcriptome Analysis Using RNA-Seq Data

BioMed Research International ◽

10.1155/2018/4037865 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15

Author(s):

Shyh-Chuan Jwo ◽

I-Fang Chung ◽

Hsei-Wei Wang ◽

Ting-Yu Chang

Keyword(s):

Growth Factors ◽

Normal Control ◽

Intestinal Adaptation ◽

Regenerative Process ◽

Rna Seq ◽

Bioinformatics Analyses ◽

Control Tissue ◽

Point Versus ◽

Potential Applications ◽

Pathway Analyses

Using our novel surgical model of simultaneous intestinal adaptation “A” and neointestinal regeneration “N” conditions in individual rats to determine feasibility for research and clinical application, we further utilized next generation RNA sequencing (RNA-Seq) here in normal control tissue and both conditions (“A” and “N”) across time to decipher transcriptome changes in neoregeneration and adaptation of intestinal tissue at weeks 1, 4, and 12. We also performed bioinformatics analyses to identify key growth factors for improving intestinal adaptation and neointestinal regeneration. Our analyses indicate several interesting phenomena. First, Gene Ontology and pathway analyses indicate that cell cycle and DNA replication processes are enhanced in week 1 “A”; however, in week 1 “N”, many immune-related processes are involved. Second, we found some growth factors upregulated or downregulated especially in week 1 “N” versus “A”. Third, based on each condition and time point versus normal control tissue, we found in week 1 “N” BMP2, BMP3, and NTF3 are significantly and specifically downregulated, indicating that the regenerative process may be inhibited in the absence of these growth factors. This study reveals complex growth factor regulation in small neointestinal regeneration and intestinal adaptation and provides potential applications in tissue engineering by introducing key growth factors identified here into the injury site.

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Enhanced periodontal regeneration using collagen stem cells or growth factors

Frontiers in Bioscience ◽

10.2741/s482 ◽

2017 ◽

Vol 9 (1) ◽

pp. 180-193 ◽

Cited By ~ 3

Author(s):

Tanja Basan

Keyword(s):

Stem Cells ◽

Growth Factors ◽

Periodontal Regeneration

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Systematic review of the use of growth factors in periodontal regeneration

Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie ◽

10.1007/s00056-012-0114-z ◽

2012 ◽

Vol 73 (6) ◽

pp. 425-429 ◽

Cited By ~ 1

Author(s):

U. Fritz

Keyword(s):

Systematic Review ◽

Growth Factors ◽

Periodontal Regeneration

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Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

Molecules ◽

10.3390/molecules25040978 ◽

2020 ◽

Vol 25 (4) ◽

pp. 978 ◽

Cited By ~ 4

Author(s):

Mariana I. Neves ◽

Marco Araújo ◽

Lorenzo Moroni ◽

Ricardo M.P. da Silva ◽

Cristina C. Barrias

Keyword(s):

Mechanical Properties ◽

Extracellular Matrix ◽

Biological Activity ◽

Growth Factors ◽

Mechanical Stability ◽

Biological Molecules ◽

Inhibiting Factors ◽

Wide Range ◽

Different Sources

Glycosaminoglycans (GAG) are long, linear polysaccharides that display a wide range of relevant biological roles. Particularly, in the extracellular matrix (ECM) GAG specifically interact with other biological molecules, such as growth factors, protecting them from proteolysis or inhibiting factors. Additionally, ECM GAG are partially responsible for the mechanical stability of tissues due to their capacity to retain high amounts of water, enabling hydration of the ECM and rendering it resistant to compressive forces. In this review, the use of GAG for developing hydrogel networks with improved biological activity and/or mechanical properties is discussed. Greater focus is given to strategies involving the production of hydrogels that are composed of GAG alone or in combination with other materials. Additionally, approaches used to introduce GAG-inspired features in biomaterials of different sources will also be presented.

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Liver Regeneration: Analysis of the Main Relevant Signaling Molecules

Mediators of Inflammation ◽

10.1155/2017/4256352 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 41

Author(s):

Yachao Tao ◽

Menglan Wang ◽

Enqiang Chen ◽

Hong Tang

Keyword(s):

Growth Factors ◽

Liver Regeneration ◽

Normal Liver ◽

Initial Step ◽

Signaling Molecules ◽

Regenerative Process ◽

Liver Mass ◽

Termination Phase ◽

And Function ◽

Stimulation Of

Liver regeneration is a highly organized tissue regrowth process and is the most important reaction of the liver to injury. The overall process of liver regeneration includes three phases: priming stage, proliferative phase, and termination phase. The initial step aims to induce hepatocytes to be sensitive to growth factors with the aid of some cytokines, including TNF-α and IL-6. The proliferation phase promotes hepatocytes to re-enter G1 with the stimulation of growth factors. While during the termination stage, hepatocytes will discontinue to proliferate to maintain normal liver mass and function. Except for cytokine- and growth factor-mediated pathways involved in regulating liver regeneration, new substances and technologies emerge to influence the regenerative process. Here, we reviewed novel and important signaling molecules involved in the process of liver regeneration to provide a cue for further research.

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Stem Cells for Periodontal Regeneration

Balkan Journal of Medical Genetics ◽

10.2478/bjmg-2013-0012 ◽

2013 ◽

Vol 16 (1) ◽

pp. 7-11 ◽

Cited By ~ 10

Author(s):

A. Pejcic ◽

D. Kojovic ◽

D. Mirkovic ◽

I. Minic

Keyword(s):

Stem Cells ◽

Growth Factors ◽

Surgical Procedures ◽

Adult Stem Cells ◽

Periodontal Regeneration ◽

Human Stem Cells ◽

Barrier Membranes ◽

Periodontal Tissues ◽

Grafting Materials ◽

Potential Use

Abstract Periodontal regeneration is considered to be biologically possible but clinically unpredictable. In periodontitis, inflammation manifests clinically as loss of supporting periodontal tissues and regeneration of damaged tissue is the main goal of treatment. For decades, periodontists have sought to repair the damage through a variety of surgical procedures, and use of grafting materials and growth factors, and of barrier membranes. Reports have emerged that demonstrate which populations of adult stem cells reside in the periodontal ligaments of humans and other animals. This opens the way for new cell-based therapies for perio-dontal regeneration. This review provides an overview of adult human stem cells and their potential use in perio-dontal regeneration.

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