scholarly journals Primary Processing and Storage Affect the Dominant Microbiota of Fresh and Chill-Stored Sea Bass Products

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 671
Author(s):  
Faidra Syropoulou ◽  
Foteini F. Parlapani ◽  
Stefanos Kakasis ◽  
George-John E. Nychas ◽  
Ioannis S. Boziaris
Keyword(s):  
Shelf Life ◽  
Microbial Communities ◽  
Pathogenic Bacteria ◽  
Sea Bass ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Primary Processing ◽  
Shewanella Species ◽  
Processing And Storage ◽  
And Storage

The cultivable microbiota isolated from three sea bass products (whole, gutted, and filleted fish from the same batch) during chilled storage and the effect of primary processing on microbial communities in gutted and filleted fish were studied. Microbiological and sensory changes were also monitored. A total of 200 colonies were collected from TSA plates at the beginning and the end of fish shelf-life, differentiated by High Resolution Sequencing (HRM) and identified by sequencing analysis of the V3–V4 region of the 16S rRNA gene. Pseudomonas spp. followed by potential pathogenic bacteria were initially found, while Pseudomonasgessardii followed by other Pseudomonas or Shewanella species dominated at the end of fish shelf-life. P. gessardii was the most dominant phylotype in the whole sea bass, P. gessardii and S. baltica in gutted fish, while P. gessardii and P. fluorescens were the most dominant bacteria in sea bass fillets. To conclude, primary processing and storage affect microbial communities of gutted and filleted fish compared to the whole fish. HRM analysis can easily differentiate bacteria isolated from fish products and reveal the contamination due to handling and/or processing, and so help stakeholders to immediately tackle problems related with microbial quality or safety of fish.

scholarly journals Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities

2020 ◽  
Vol 8 (6) ◽  
pp. 919 ◽  
Author(s):  
Huichuan Zhuang ◽  
Zhuoying Wu ◽  
Linji Xu ◽  
Shao-Yuan Leu ◽  
Po-Heng Lee
Keyword(s):  
Dissolved Oxygen ◽  
Microbial Communities ◽  
Energy Efficient ◽  
Oxygen Demand ◽  
Operating Conditions ◽  
Single Stage ◽  
Rrna Gene ◽  
Unexpected Finding ◽  
Sequencing Analysis ◽  
Ratio Requirement

Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20 °C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing Haematobacter and nitrogen-fixing Geofilum and Shinella were highly abundant with the copredominance of ammonia-oxidizing Comamonas and Nitrosomonas, nitrite-oxidizing Limnohabitans, and denitrifying Simplicispira, Castellaniella, and Nitratireductor. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment.

scholarly journals KAJIAN RETENSI KAROTEN KAPANG ONCOM MERAH DARI TONGKOL JAGUNG SELAMA PENGOLAHAN DAN PENYIMPANAN MIE INSTAN FUNGSIONAL

KOVALEN ◽  
2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Nurakhirawati Nurakhirawati ◽  
Harianthy Aneke ◽  
Syaiful Bahri
Keyword(s):  
Shelf Life ◽  
Reaction Kinetics ◽  
Treatment Phase ◽  
Kinetics Model ◽  
Temperature Analysis ◽  
Processing And Storage ◽  
And Storage ◽  
Instant Noodle ◽  
Instant Noodles

Has done research on carotenoids retention oncom red on the processing and storage of instant noodles functional.This study aims was to determine the level of damage carotene in the instant noodle processing and to determine the carotene retention during storage of instant noodles as well as to determine the shelf life of instant noodles at a temperature 50ºC  and 60ºC. Estimation of shelf life or expiration period using the model of reaction kinetics the treatment phase of the study include red oncom mold production, processing instant noodles and storage of instant noodles on the temperature. Analysis carotene on instant noodlesdone every 0 days to 6 days using UV-Vis spectrophotometry method in wavelength. The data obtained is used to determine the shelf life of instant noodles. The results obtained showed live carotene demage during  the processing of instant noodles is to 20g = 1,867%. For temperature 60oC for 20g = 10,208%, and determining Q10 is 2,90 and the expiration of a functional instant noodles is 122 days.Keywords : Carotene, Mie Functional, Retention carotene, Shelf life, Reaction Kinetics Model

scholarly journals Microbial Profiles of Retail Pacific Oysters (Crassostrea gigas) From Guangdong Province, China

2021 ◽  
Vol 12 ◽  
Author(s):  
Mingjia Yu ◽  
Xiaobo Wang ◽  
Aixian Yan
Keyword(s):  
Microbial Communities ◽  
Crassostrea Gigas ◽  
Pathogenic Bacteria ◽  
High Throughput Sequencing ◽  
Vibrio Vulnificus ◽  
Foodborne Pathogen ◽  
Guangdong Province ◽  
Sequencing Analysis ◽  
Alpha And Beta Diversity ◽  
Pacific Oysters

Oysters are one of the main aquatic products sold in coastal areas worldwide and are popular among consumers because of their delicious taste and nutritional value. However, the microorganisms present in oysters may pose health risks to consumers. In this study, the microbial communities of Pacific oysters (Crassostrea gigas) collected from aquatic product markets in three cities (Guangzhou, Zhuhai, and Jiangmen) of Guangdong Province, China, where raw oysters are popular, were investigated. The plate counts of viable bacteria in oysters collected in the three cities were all approximately 2 log colony-forming units/g. High-throughput sequencing analysis of the V3–V4 region of the 16Sribosomal DNA gene showed a high level of microbial diversity in oysters, as evidenced by both alpha and beta diversity analysis. Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla of the microorganisms present in these samples. A variety of pathogenic bacteria, including the fatal foodborne pathogen Vibrio vulnificus, were found, and Vibrio was the dominant genus. Additionally, the relationship between other microbial species and pathogenic microorganisms may be mostly symbiotic in oysters. These data provide insights into the microbial communities of retail oysters in the Guangdong region and indicate a considerable risk related to the consumption of raw oysters.

scholarly journals Dynamics and Heterogeneity of Microbial Communities in Urban Water Environments

2020 ◽  
Author(s):  
Yanqing Lian ◽  
Lisha Zhen ◽  
Xi Chen ◽  
Yang Li ◽  
Xiaona Li
Keyword(s):  
Microbial Communities ◽  
Urban Areas ◽  
Pathogenic Bacteria ◽  
Temporal Dynamics ◽  
Statistical Analyses ◽  
Polluted Water ◽  
Outer Side ◽  
Rrna Gene ◽  
The City ◽  
The 16S Rrna Gene

Abstract Water samples for the 16S rRNA gene and water quality analyses were collected from around 155 kilometers of river segments surrounding the urban areas in Xi’an of China. Multiple statistical analyses showed the temporal dynamics of microbial communities and heterogeneity in their spatial distributions. The dynamic shifts of microbial communities in the Chan, Ba, and Feng Rivers from the Spring to the Summer seasons were apparent, but little in the Zao River. The heterogeneity of microbial distributions was more due to the influence of hydrologic conditions and various sources of inflows in the rivers. The LEfSe analysis showed the Chan and Zao Rivers, both were more impacted by the sewage effluents, were more differentially abundant with bacteria related to polluted water, but the Ba and Feng Rivers, both on the outer side of the city, were more abundant with microbial communities in soil and freshwater environments in August. Multiple statistical analyses indicated that environmental variables had a significant impact on microbial communities. The GIS-based spatial analysis not only showed heterogeneity of microbial community distributions along the rivers, more importantly, could help identify locations where pathogenic bacteria presented.

Bacterial Distribution and Community Structure in Beef Cattle Liver and Bile at Slaughter

2021 ◽  
Author(s):  
Hiroshi Asakura ◽  
Shiori Yamamoto ◽  
Yoshimasa Sasaki ◽  
Yumiko Okada ◽  
Sachiko Katabami ◽  
...  
Keyword(s):  
Community Structure ◽  
Liver Parenchyma ◽  
Indicator Bacteria ◽  
Microbiological Analysis ◽  
Sequencing Analysis ◽  
Fecal Indicator ◽  
Semiconductor Sequencing ◽  
Bacterial Transmission ◽  
Processing And Storage ◽  
And Storage

In this study, the distribution of hygienic indicator bacteria in cattle livers and bile was examined at slaughterhouses. First, 127 cattle livers with gallbladders were carefully eviscerated from the carcasses at 10 slaughterhouses. Microbiological examination showed that 9 bile (7.1%) and 19 liver parenchyma (15.0%) samples were positive for the family Enterobacteriaceae (EB) with means ± SD of 3.68 ± 4.63 log CFU/mL and 1.59 ± 2.47 log CFU/g, respectively; thus, bacterial contamination was apparent even at the postevisceration stage. Subsequently, 70 cattle livers were obtained at the postprocessing/storage stage from 7 of the ten slaughterhouses; microbiological analysis revealed greater means of EB in the liver parenchyma (means ± SD of 3.00 ± 3.89 log CFU/g, P =0.011) than those at postevisceration stage, suggesting that bacterial dissemination and/or replication occurred in the liver parenchyma during processing and storage. According to 16S rRNA ion semiconductor sequencing analysis of representative samples from 12 cattle, Proteobacteria , Firmicutes , and Actinobacteria were dominant in both the parenchyma and bile, in which EB/ Escherichia coli were predominate among EB-rich livers. These results suggest that bile plays a role as a vehicle for bacterial transmission to the liver parenchyma. This is the first study to demonstrate bacterial distribution and community structure in the liver and biliary microecosystem of cattle at slaughter. Our data provide possible implication of EB testing in bile to screen cattle livers contaminated with high levels of fecal indicator bacteria.

scholarly journals Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202049 ◽  
Author(s):  
Nobuo Watanabe ◽  
Kirill Kryukov ◽  
So Nakagawa ◽  
Junko S. Takeuchi ◽  
Meiko Takeshita ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Pathogenic Bacteria ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Sequencing Analysis
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