Bacterial Distribution and Community Structure in Beef Cattle Liver and Bile at Slaughter

2021 ◽  
Author(s):  
Hiroshi Asakura ◽  
Shiori Yamamoto ◽  
Yoshimasa Sasaki ◽  
Yumiko Okada ◽  
Sachiko Katabami ◽  
...  
Keyword(s):  
Community Structure ◽  
Liver Parenchyma ◽  
Indicator Bacteria ◽  
Microbiological Analysis ◽  
Sequencing Analysis ◽  
Fecal Indicator ◽  
Semiconductor Sequencing ◽  
Bacterial Transmission ◽  
Processing And Storage ◽  
And Storage

In this study, the distribution of hygienic indicator bacteria in cattle livers and bile was examined at slaughterhouses. First, 127 cattle livers with gallbladders were carefully eviscerated from the carcasses at 10 slaughterhouses. Microbiological examination showed that 9 bile (7.1%) and 19 liver parenchyma (15.0%) samples were positive for the family Enterobacteriaceae (EB) with means ± SD of 3.68 ± 4.63 log CFU/mL and 1.59 ± 2.47 log CFU/g, respectively; thus, bacterial contamination was apparent even at the postevisceration stage. Subsequently, 70 cattle livers were obtained at the postprocessing/storage stage from 7 of the ten slaughterhouses; microbiological analysis revealed greater means of EB in the liver parenchyma (means ± SD of 3.00 ± 3.89 log CFU/g, P =0.011) than those at postevisceration stage, suggesting that bacterial dissemination and/or replication occurred in the liver parenchyma during processing and storage. According to 16S rRNA ion semiconductor sequencing analysis of representative samples from 12 cattle, Proteobacteria , Firmicutes , and Actinobacteria were dominant in both the parenchyma and bile, in which EB/ Escherichia coli were predominate among EB-rich livers. These results suggest that bile plays a role as a vehicle for bacterial transmission to the liver parenchyma. This is the first study to demonstrate bacterial distribution and community structure in the liver and biliary microecosystem of cattle at slaughter. Our data provide possible implication of EB testing in bile to screen cattle livers contaminated with high levels of fecal indicator bacteria.

scholarly journals Primary Processing and Storage Affect the Dominant Microbiota of Fresh and Chill-Stored Sea Bass Products

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 671
Author(s):  
Faidra Syropoulou ◽  
Foteini F. Parlapani ◽  
Stefanos Kakasis ◽  
George-John E. Nychas ◽  
Ioannis S. Boziaris
Keyword(s):  
Shelf Life ◽  
Microbial Communities ◽  
Pathogenic Bacteria ◽  
Sea Bass ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Primary Processing ◽  
Shewanella Species ◽  
Processing And Storage ◽  
And Storage

The cultivable microbiota isolated from three sea bass products (whole, gutted, and filleted fish from the same batch) during chilled storage and the effect of primary processing on microbial communities in gutted and filleted fish were studied. Microbiological and sensory changes were also monitored. A total of 200 colonies were collected from TSA plates at the beginning and the end of fish shelf-life, differentiated by High Resolution Sequencing (HRM) and identified by sequencing analysis of the V3–V4 region of the 16S rRNA gene. Pseudomonas spp. followed by potential pathogenic bacteria were initially found, while Pseudomonasgessardii followed by other Pseudomonas or Shewanella species dominated at the end of fish shelf-life. P. gessardii was the most dominant phylotype in the whole sea bass, P. gessardii and S. baltica in gutted fish, while P. gessardii and P. fluorescens were the most dominant bacteria in sea bass fillets. To conclude, primary processing and storage affect microbial communities of gutted and filleted fish compared to the whole fish. HRM analysis can easily differentiate bacteria isolated from fish products and reveal the contamination due to handling and/or processing, and so help stakeholders to immediately tackle problems related with microbial quality or safety of fish.

scholarly journals Assessing Market-Sold Remedies in Lomé (Togo) for Hygienic Quality

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Comlan de Souza ◽  
Yaovi Ameyapoh ◽  
Simplice D. Karou ◽  
Kokou T. Anani ◽  
Madje L. Kpodar ◽  
...  
Keyword(s):  
Aerobic Bacteria ◽  
Microbiological Analysis ◽  
Total Coliforms ◽  
Microbial Risk ◽  
Thermotolerant Coliforms ◽  
Plant Processing ◽  
Processing And Storage ◽  
Reducing Bacteria ◽  
And Storage

Traditional concoctions sold in marketplaces are always assumed to be safe and efficient; however, they can be potentially toxic because of poor hygienic practices in plant processing or storage. The present study aimed to assess for the microbial quality of market-sold vegetable drugs in Lomé. Thus, a total of 209 plant remedies were collected in marketplaces and analysed for the presence of total aerobic bacteria, total coliforms, thermotolerant coliforms, Staphylococcus aureus, sulphite reducing bacteria, and yeast and moulds according to the French Association of Normalisation (AFNOR) guidelines. The results revealed that all formulations were contaminated by several microorganisms, excepted alcohol-based mixtures. According to AFNOR limits nonconform drugs were according to total aerobic bacteria (86.96% powders, 81.82% capsules, 66.67% tisanes, and 42.11% decoctions); to total coliforms (9.10% capsules, 8.70% powders and 1.75% decoction); to yeasts and moulds (77.78% ointments, 40% calcined powders, 36.36% capsules, and 23.91% powders). The microbiological analysis revealed that the majority of contaminating bacteria were gram positive catalase positive and oxidase positive bacilli. Quality control studies on market-sold remedies are currently needed to evaluate the microbial risk in consuming these products and they may allow the standardisation of plant processing and storage.

HUBUNGAN HIGIENE SANITASI DENGAN KANDUNGAN ESCHERICHIA COLI PADA ES BATU INDUSTRI RUMAH TANGGA DI TEPI JALAN WAHID HASYIM 2 KECAMATAN SEMPAJA KOTA SAMARINDA TAHUN 2015

2018 ◽  
Vol 2 (1) ◽  
pp. 43
Author(s):  
Suwignyo Suwignyo ◽  
Abdul Rachim ◽  
Arizal Sapitri
Keyword(s):  
Escherichia Coli ◽  
Data Analysis ◽  
Random Sampling ◽  
Preliminary Test ◽  
Raw Material ◽  
Almost Everywhere ◽  
Ice Cube ◽  
Processing And Storage ◽  
And Storage ◽  
Cluster Random Sampling

Ice is a water that cooled below 0 °C and used for complement in drink. Ice can be found almost everywhere, including in the Wahid Hasyim Sempaja Roadside. From the preliminary test, obtained 5 samples ice cube were contaminated by Escherichia coli. The purpose of this study was to determine relationship between hygiene and sanitation with presence of Eschericia coli in ice cube of home industry at Wahid Hasyim Roadside Samarinda. This research used quantitative with survey methode. The population in this study was all of the seller in 2nd Wahid Hasyim Roadside. Sample was taken by Krejcie and Morgan so the there were 44 samples and used Cluster Random Sampling. The instruments are questionnaries, observation and laboratory test. Data analysis was carried out univariate and bivariate (using Fisher test p= 0.05). The conclusion of this study there are a relation between chosing raw material (p=0,03) and saving raw material (p=0,03) with presence of Eschericia coli. There was no relation between processing raw material into ice cube with presence of Eschericia coli (p=0,15).Advice that can be given to ice cube should maintain hygiene and sanitation of the selection, processing and storage of ice cube.

Prospects for the development of agrarian production systems in in the Right Bank Polesie of Ukraine

2018 ◽  
Vol 2 (95) ◽  
pp. 69-72
Author(s):  
Yu.A. Tarariko ◽  
L.V. Datsko ◽  
M.O. Datsko
Keyword(s):  
Agricultural Production ◽  
Production Systems ◽  
Economic Feasibility ◽  
Capital Investments ◽  
Resource Potential ◽  
Grain Processing ◽  
Processing And Storage ◽  
The Right ◽  
And Storage ◽  
Agricultural Production Systems

The aim of the work is to assess the existing and prospective models for the development of agricultural production in Central Polesie on the basis of economic feasibility and ecological balance. The evaluation of promising agricultural production systems was carried out with the help of simulation modeling of various infrastructure options at the levels of crop and multisectoral specialization of agroecosystems. The agro-resource potential of Central Polesie is better implemented in the rotation with lupine, corn and flax dolguntsem with well-developed infrastructure, including crop, livestock units, grain processing and storage systems, feed, finished products and waste processing in the bioenergetic station. The expected income for the formation of such an infrastructure is almost 8 thousand dollars. / with a payback period of capital investments of 2-3 years.

Fish, Fires, and Foresight: Middle Woodland Economic Adaptations in the Abbott Farm National Landmark

1984 ◽  
Vol 5 (2) ◽  
pp. 111-138 ◽  
Author(s):  
John A. Cavallo
Keyword(s):  
New Jersey ◽  
Raw Material ◽  
Anadromous Fish ◽  
Middle Woodland ◽  
Cultural Resource ◽  
Economic Adaptation ◽  
Processing And Storage ◽  
And Storage ◽  
Regional Redistribution

The “Abbott Farm site,” now known as the Abbott Farm National Landmark, is known for its unique and abundant artifact assemblages from presumed Middle Woodland contexts. Several researchers attributed the presence of large quantities of argillite raw material, argillite and chert blades and blade caches, cut and uncut mica, and aberrant zone-decorated ceramics to either Hopewellian manifestations or to the Abbott Farm's role in the regional redistribution of argillite. On the basis of a cultural resource survey conducted by Louis Berger and Associates prior to the proposed construction of I-195 and I-295 and New Jersey Routes 29 and 129, a specialized economic adaptation focused upon the intensive procurement, processing, and storage of anadromous fish, especially sturgeon, is hypothesized.

Changes in volatile compounds, flavour-related enzymes and lycopene in a refrigerated tomato juice during processing and storage

2021 ◽  
Author(s):  
Nieves Baenas ◽  
Sergio Bravo ◽  
Francisco Javier García-Alonso ◽  
José Vicente Gil ◽  
María Jesús Periago
Keyword(s):  
Volatile Compounds ◽  
Tomato Juice ◽  
Processing And Storage ◽  
And Storage
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