scholarly journals Building of an Internal Transcribed Spacer (ITS) Gene Dataset to Support the Italian Health Service in Mushroom Identification

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1193
Author(s):  
Alice Giusti ◽  
Enrica Ricci ◽  
Laura Gasperetti ◽  
Marta Galgani ◽  
Luca Polidori ◽  
...  
Keyword(s):  
Health Service ◽  
National Level ◽  
Its Sequences ◽  
Target Species ◽  
Blast Analysis ◽  
Official Control ◽  
Its Gene ◽  
Reference Sequences ◽  
Set Up ◽  
Italian Territory

This study aims at building an ITS gene dataset to support the Italian Health Service in mushroom identification. The target species were selected among those mostly involved in regional (Tuscany) poisoning cases. For each target species, all the ITS sequences already deposited in GenBank and BOLD databases were retrieved and accurately assessed for quality and reliability by a systematic filtering process. Wild specimens of target species were also collected to produce reference ITS sequences. These were used partly to set up and partly to validate the dataset by BLAST analysis. Overall, 7270 sequences were found in the two databases. After filtering, 1293 sequences (17.8%) were discarded, with a final retrieval of 5977 sequences. Ninety-seven ITS reference sequences were obtained from 76 collected mushroom specimens: 15 of them, obtained from 10 species with no sequences available after the filtering, were used to build the dataset, with a final taxonomic coverage of 96.7%. The other 82 sequences (66 species) were used for the dataset validation. In most of the cases (n = 71; 86.6%) they matched with identity values ≥ 97–100% with the corresponding species. The dataset was able to identify the species involved in regional poisoning incidents. As some of these species are also involved in poisonings at the national level, the dataset may be used for supporting the National Health Service throughout the Italian territory. Moreover, it can support the official control activities aimed at detecting frauds in commercial mushroom-based products and safeguarding consumers.

scholarly journals Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shan Shen ◽  
Shi-Liang Liu ◽  
Ji-Hang Jiang ◽  
Li-Wei Zhou
Keyword(s):  
Species Identification ◽  
Rolling Circle Amplification ◽  
Practical Method ◽  
Morphological Characters ◽  
Its Sequences ◽  
Commercial Development ◽  
Medicinal Mushrooms ◽  
Rolling Circle ◽  
Reference Sequences ◽  
Its Barcoding

Abstract“Sanghuang” refers to a group of important traditionally-used medicinal mushrooms belonging to the genus Sanghuangporus. In practice, species of Sanghuangporus referred to in medicinal studies and industry are now differentiated mainly by a BLAST search of GenBank with the ITS barcoding region as a query. However, inappropriately labeled ITS sequences of “Sanghuang” in GenBank restrict accurate species identification and, to some extent, the utilization of these species as medicinal resources. We examined all available 271 ITS sequences related to “Sanghuang” in GenBank including 31 newly submitted sequences from this study. Of these sequences, more than half were mislabeled so we have now corrected the corresponding species names. The mislabeled sequences mainly came from strains utilized by non-taxonomists. Based on the analyses of ITS sequences submitted by taxonomists as well as morphological characters, we separate the newly described Sanghuangporus subbaumii from S. baumii and treat S. toxicodendri as a later synonym of S. quercicola. Fourteen species of Sanghuangporus are accepted, with intraspecific distances up to 1.30% (except in S. vaninii, S. weirianus and S. zonatus) and interspecific distances above 1.30% (except between S. alpinus and S. lonicerinus, and S. baumii and S. subbaumii). To stabilize the concept of these 14 species of Sanghuangporus, their taxonomic information and reliable ITS reference sequences are provided. Moreover, ten potential diagnostic sequences are provided for Hyperbranched Rolling Circle Amplification to rapidly confirm three common commercial species, viz. S. baumii, S. sanghuang, and S. vaninii. Our results provide a practical method for ITS barcoding-based species identification of Sanghuangporus and will promote medicinal studies and commercial development from taxonomically correct material.

We Are All Environmentalists! Framing Life in the National Green Tribunal, India

2021 ◽  
pp. 0169796X2110012
Author(s):  
Sudha Vasan
Keyword(s):  
National Level ◽  
Himachal Pradesh ◽  
Indigenous Community ◽  
Environmental Laws ◽  
Set Up ◽  
Power Project

India has set up one of the first national-level legal bodies, the National Green Tribunal (NGT), dedicated exclusively to address cases under environmental laws. My research follows a case filed in the NGT by an indigenous community against a hydel power project in the western Himalayan state of Himachal Pradesh, examining how diverse and opposing parties in this case represent themselves as environmentalists. It reveals a narrative sphere where entirely opposite actions and actors are legitimated in and through the NGT in environmental terms. This article suggests that green courts provoke green narratives and examines how diverse actors respond and engage with this demand. Individuals are interpellated in this juridical field to understand and present themselves as environmentalists. Environment is a meta-narrative in this juridical field, constituting environmentalist subjectivity of all actors within this field by the very process of hailing them.

The Role of the Chularajmontri (Shaykh al-IslOEm) in Resolving Ethno-religious Conflict in Southern Thailand

2010 ◽  
Vol 27 (1) ◽  
pp. 31-53
Author(s):  
Imtiyaz Yusuf
Keyword(s):  
Significant Role ◽  
National Level ◽  
Religious Conflict ◽  
Nation State ◽  
Religious Nationalism ◽  
Muslim Community ◽  
Southern Thailand ◽  
New Nation ◽  
Set Up

The century-old conflict in southern Thailand, which began with Siam’s annexation of the former Malay sultanate of Negara Patani in 1909, reemerged viciously in 2004 – with no end in sight. The Thai state expected that its official head of the Muslim community at the national level, the chularajmontri (shaykh al-Islam), whose office was set up in 1945 to integrate all Thai Muslims into the new nation-state of Thailand (formerly called Siam), would lay a significant role in resolving the southern conflict. Thus, this office was entrusted with tackling the issue of ethno-religious nationalism among the southern Muslims, an important factor lying at the root of this conflict. The office was expected to address the Thai nation-state’s political and socio-religious needs via promoting a pro-integration religious interpretation of Islam. This paper contends that its failure to contribute toward the conflict’s resolution lies in the differences in the two parties’ historical, ethnic, and religious interpretations of Islam.

scholarly journals Who is the right person to look after the mentally retarded?

Curationis ◽  
1980 ◽  
Vol 3 (3) ◽  
Author(s):  
V. Ehlers
Keyword(s):  
National Health Service ◽  
Health Service ◽  
Residential Care ◽  
National Health ◽  
Mentally Retarded ◽  
Nursing Profession ◽  
Financial Resources ◽  
Mentally Handicapped ◽  
Set Up ◽  
The Right

A committee was set up in Britain in 1975 under the Chairmanship of Mrs Peggy Jay to look into the staffing of mental handicapped residential care in the National Health Service. Part of the task was to consider the Briggs Committee’s recommendation that “… a new caring profession for the mentally handicapped should emerge gradually”. The findings and recommendations of the committee were however radical and far-reaching, involving an enormous shift in financial resources and causing much concern and outcry from the nursing profession which considered the new category of care given as a threat to their existence.

Infrastructure Provisioning and Health Service Utilization in Africa

2017 ◽  
Vol 3 (1) ◽  
pp. 47-69 ◽  
Author(s):  
Amm Quamruzzaman
Keyword(s):  
Health Service ◽  
Service Utilization ◽  
Health Service Utilization ◽  
Access To Health Care ◽  
National Level ◽  
Health Sector ◽  
Local Health ◽  
African Countries ◽  
Individual Level ◽  
Different Levels

Although the positive developmental effects of infrastructure provisioning are well documented, research on the potential role of governance in the improvement of infrastructure performance and individual-level service utilization is lacking. I explore the effect of infrastructure provisioning on individual-level health service utilization, paying close attention to whether governance at different levels shapes people's access to health care. The different geographical levels of infrastructure provisioning, governance, and health service utilization require a multilevel analysis, which I perform using Afrobarometer Round 5 survey data on 34 African countries in a three-stage mixed-effects modeling. Results show that the presence of health infrastructure is crucial for enhancing people's health service utilization. However, people encounter certain problems when receiving services at their local health clinics or hospitals, and these problems are directly linked with governance in the health sector as well as overall governance at the country level. Improvements in people's health service utilization therefore require both better infrastructure provisioning and better governance at different levels, as the former does not guarantee the latter. Development scholars need to widen their focus beyond national-level governance and help policy makers identify at which level state interventions are most needed for removing barriers to development.

scholarly journals Changes And New Constraints In The Competition Rules Enforcement Due To Accession: The Perception Of The Romanian Companies

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Alina Mihaela Dima
Keyword(s):  
Competition Policy ◽  
Negative Impact ◽  
Free Market ◽  
National Level ◽  
Well Being ◽  
Business Environment ◽  
Competition Rules ◽  
Set Up ◽  
The One ◽  
The Right

Many times, in the attempt to win or to maintain an advantageous position on the market, the economic agent will use a whole arsenal of practices (inclusively and mostly from the marketing field), most of them anticompetitive, with a negative impact on the business environment, which also affects the well-being of the consumer. The policy in the field of competition is the one that defines these types of behaviour and penalizes them depending on the importance of their negative impact, by creating a complex and coherent legislative and institutional mechanism. The right enforcement of the competition policy at the national level is the key in this process, but this should be coordinated with the regional and international objectives and regulations in this field. Romania is facing a double challenge: on the one side, it had to set up a competition policy, which was almost ignored before the90s, on the other side, it had to comply, recently, with high standards in the field, as an EU candidate. Now, as a member state, the promotion of a competition culture becomes a must, along with the design of an adequate system of information and knowledge dissemination for all of those involved. The paper is based on a original and qualitative research and aims at emphasising the increased necessity of the promotion of a competition culture for the competitiveness of the Romanian business environment on the European level in the new context of accession. This will help Romanian business to face the competition challenges within a more extended single European market, as an essential issue of the free market economy status recently granted, and accordingly to the most important EU objectives set up at Lisabon to become the most competitive economy in the world up to 2010.

scholarly journals Application of internal transcribed spacer (ITS) sequences for identifying Anoectochilus setaceus Blume in Thanh Hoa, Vietnam

2020 ◽  
Vol 181 (2) ◽  
pp. 108-116
Author(s):  
B. B. Thinh ◽  
L. D. Chac ◽  
L. T.M. Thu
Keyword(s):  
Internal Transcribed Spacer ◽  
Molecular Taxonomy ◽  
Gene Segment ◽  
Dna Barcode ◽  
Its Sequences ◽  
Gene Region ◽  
Its Gene ◽  
Ctab Method ◽  
Young Leaves ◽  
Total Dna

Background. The term “DNA barcode” is used extensively in molecular taxonomy. Basically, this technique is based on the use of a DNA sequence (about 400–800 bp) as a standard to identify and determine the species relation of organisms quickly and accurately. Therefore, DNA barcodes not only help taxonomists in classifying and identifying species, but also improve their ability to control, understand and utilize biodiversity. In this study, the authors conducted identification of samples of Anoectochilus setaceus Blume collected in Thanh Hoa through the isolated sequence of ITS gene regions.Materials and methods. Total DNA was extracted from young leaves of A. setaceus samples using CTAB method. The ITS gene segment was amplified by PCR and sequenced. This genetic sequence was analyzed, compared and used to establish a phylogenetic tree using BioEdit, BLAST and DNASTAR programs.Results and conclusion. We isolated 4 sequences of the ITS gene region in 4 A. setaceus samples collected at Xuan Lien and Pu Luong of Thanh Hoa province; the ITS gene region was 667 nucleotide long. The findings identified the samples as the same species and showed 99% similarity to the ITS gene sequence of A. roxburghii (Wall.) Lindl. published in GenBank, GQ328774. This study also demonstrates that the method employing internal transcribed spacer (ITS) sequences is an effective tool to identify A. setaceus taxa.

Competitive consensus: bargaining on employment and competitiveness in the Netherlands

2001 ◽  
Vol 7 (4) ◽  
pp. 682-696 ◽  
Author(s):  
Rien Huiskamp ◽  
Maarten van Riemsdijk
Keyword(s):  
The Netherlands ◽  
Collective Bargaining ◽  
National Level ◽  
Collective Agreements ◽  
Wide Range ◽  
Form Part ◽  
Set Up ◽  
National Framework ◽  
Insight Into ◽  
Empirical Insight

This article shows how bargaining on the conflicting issues of fighting unemployment and increasing competitiveness has evolved. It offers an empirical insight into the degree to which the national framework agreements that form part of the now famous Dutch polder model are implemented. At the national level framework agreements are set up and recommendations are made on a wide range of issues. It is shown that these are then interpreted and partly adopted by negotiators at lower collective bargaining levels. At company level, three cases illustrate differences in the degree to which companies implement the outcomes of collective agreements: from ‘dedicated follower’ to ‘rebels with a cause'. Looking at the evidence, it seems the Dutch have experienced a form of organised decentralisation.

Excellence and gender

2015 ◽  
Vol 34 (1) ◽  
pp. 37-54 ◽  
Author(s):  
Farinaz Fassa
Keyword(s):  
Gender Equality ◽  
Common Good ◽  
Design Methodology ◽  
Local Level ◽  
National Level ◽  
Academic Careers ◽  
Content Type ◽  
And Gender ◽  
Set Up ◽  
Feminist Movements

Purpose – Discussing the Swiss case, the purpose of this paper is to examine how gender equality policies deal with the present requirements for scholars to be considered “excellent”. It aims to pinpoint the lines of tension or coherence between excellence, meritocracy and gender politics. Design/methodology/approach – In order to specify the norms of academic careers and their different renditions, the author draws on two studies (at local and national levels) to illustrate where the changes and resistances are taking place. Findings – The translations of a number of demands of feminist movements into the policies set up to favour equality between the sexes may combine to challenge the norms of academia as a gendered realm. Nevertheless, without strong pressure from feminists at local level and the conduct of research pursuing the enterprise of deconstructing norms, top-down policies may prove less “corrective” than affirmative action. This pressure is not only useful to build gender equality in science but also to broaden the spectrum of knowledge that can become a common good. Research limitations/implications – As neither the names nor the positions of the experts who select the candidates at national level are made public, we had to opt for other, less satisfactory means. Originality/value – The originality of the paper lies in the link made between the enhancements brought by Equalities policies and the changes they bring. It attempts to bring to light the extent to which gender equality policies conform to the neo-managerial order or challenge its norms to build a world that is more just.

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