scholarly journals Biomapping of Microbial Indicators on Beef Subprimals Subjected to Spray or Dry Chilling over Prolonged Refrigerated Storage

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1403
Author(s):  
Diego E. Casas ◽  
Rosine Manishimwe ◽  
Savannah J. Forgey ◽  
Keelyn E. Hanlon ◽  
Markus F. Miller ◽  
...  
Keyword(s):  
Shelf Life ◽  
Hot Water ◽  
Cold Chain ◽  
Prolonged Storage ◽  
Refrigerated Storage ◽  
Indicator Organisms ◽  
Sample Collection ◽  
Significant Difference ◽  
Packaged Beef ◽  
The Impact

As the global meat market moves to never frozen alternatives, meat processors seek opportunities for increasing the shelf life of fresh meats by combinations of proper cold chain management, barrier technologies, and antimicrobial interventions. The objective of this study was to determine the impact of spray and dry chilling combined with hot water carcass treatments on the levels of microbial indicator organisms during the long-term refrigerated storage of beef cuts. Samples were taken using EZ-Reach™ sponge samplers with 25 mL buffered peptone water over a 100 cm2 area of the striploin. Sample collection was conducted before the hot carcass wash, after wash, and after the 24 h carcass chilling. Chilled striploins were cut into four sections, individually vacuum packaged, and stored to be sampled at 0, 45, 70, and 135 days (n = 200) of refrigerated storage and distribution. Aerobic plate counts, enterobacteria, Escherichia coli, coliforms, and psychrotroph counts were evaluated for each sample. Not enough evidence (p > 0.05) was found indicating the hot water wash intervention reduced bacterial concentration on the carcass surface. E. coli was below detection limits (<0.25 CFU/cm2) in most of the samples taken. No significant difference (p > 0.05) was found between coliform counts throughout the sampling dates. Feed type did not seem to influence the (p > 0.25) microbial load of the treatments. Even though no immediate effect was seen when comparing spray or dry chilling of the samples at day 0, as the product aged, a significantly lower (p < 0.05) concentration of aerobic and psychrotrophic organisms in dry-chilled samples could be observed when compared to their spray-chilled counterparts. Data collected can be used to select alternative chilling systems to maximize shelf life in vacuum packaged beef kept over prolonged storage periods.

scholarly journals Effect of Chitosan Coating Incorporated with Artemisia fragrans Essential Oil on Fresh Chicken Meat during Refrigerated Storage

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 716
Author(s):  
Milad Yaghoubi ◽  
Ali Ayaseh ◽  
Kazem Alirezalu ◽  
Zabihollah Nemati ◽  
Mirian Pateiro ◽  
...  
Keyword(s):  
Essential Oil ◽  
Shelf Life ◽  
Thiobarbituric Acid ◽  
Chicken Meat ◽  
Refrigerated Storage ◽  
Quality Properties ◽  
Overall Acceptability ◽  
Chitosan Coating ◽  
The Impact ◽  
Organoleptic Attributes

The present study was conducted to assess the impact of chitosan coating (1%) containing Artemisia fragrans essential oil (500, 1000, and 1500 ppm) as antioxidant and antimicrobial agent on the quality properties and shelf life of chicken fillets during refrigerated storage. After packaging meat samples, physicochemical, microbiological, and organoleptic attributes were evaluated at 0, 3, 6, 9, and 12 days at 4 °C. The results revealed that applied chitosan (CH) coating in combination with Artemisia fragrans essential oils (AFEOs) had no significant (p < 0.05) effects on proximate composition among treatments. The results showed that the incorporation of AFEOs into CH coating significantly reduced (p < 0.05) pH, thiobarbituric acid reactive substances (TBARS), and total volatile base nitrogen (TVB-N), especially for 1% CH coating + 1500 ppm AFEOs, with values at the end of storage of 5.58, 1.61, and 2.53, respectively. The coated samples also displayed higher phenolic compounds than those obtained by uncoated samples. Coated chicken meat had, significantly (p < 0.05), the highest inhibitory effects against microbial growth. The counts of TVC (total viable counts), coliforms, molds, and yeasts were significantly lower (p < 0.05) in 1% CH coating + 1500 ppm AFEOs fillets (5.32, 3.87, and 4.27 Log CFU/g, respectively) at day 12. Organoleptic attributes of coated samples also showed the highest overall acceptability scores than uncoated ones. Therefore, the incorporation of AFEOs into CH coating could be effectively used for improving stability and shelf life of chicken fillets during refrigerated storage.

scholarly journals Using a concentrate of phenols obtained from olive vegetation water to preserve chilled food: two case studies

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Luca Fasolato ◽  
Barbara Cardazzo ◽  
Stefania Balzan ◽  
Lisa Carraro ◽  
Nadia Andrea Andreani ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Shelf Life ◽  
Sparus Aurata ◽  
Chicken Breast ◽  
Lag Phase ◽  
Plant Metabolites ◽  
Significant Difference ◽  
Vegetation Water ◽  
Fresh Products ◽  
The Impact

Phenols are plant metabolites characterised by several interesting bioactive properties such as antioxidant and bactericidal activities. In this study the application of a phenols concentrate (PC) from olive vegetation water to two different fresh products – gilt-head seabream (<em>Sparus aurata</em>) and chicken breast – was described. Products were treated in a bath of PC (22 g/L; chicken breast) or sprayed with two different solutions (L1:0.75 and L2:1.5 mg/mL; seabream) and then stored under refrigeration conditions. The shelf life was monitored through microbiological analyses – quality index method for seabream and a specific sensory index for raw breast. The secondary products of lipid-peroxidation of the chicken breast were determined using the thiobarbituric acid reactive substances (TBARs) test on cooked samples. Multivariate statistical techniques were adopted to investigate the impact of phenols and microbiological data were fitted by DMfit software. In seabream, the levels of PC did not highlight any significant difference on microbiological and sensory features. DMfit models suggested an effect only on H<sub>2</sub>S producing bacteria with an increased lag phase compared to the control samples (C: 87 h <em>vs</em> L2: 136 h). The results on chicken breast showed that the PC bath clearly modified the growth of <em>Pseudomonas</em> and <em>Enterobacteriaceae</em>. The phenol dipping was effective in limiting lipid-peroxidation (TBARs) after cooking. Treated samples disclosed an increase of shelf life of 2 days. These could be considered as preliminary findings suggesting the use of this concentrate as preservative in some fresh products.

Coating with chitosan containing lauric acid (C12:0) significantly extends the shelf-life of aerobically – Packaged beef steaks during refrigerated storage

Meat Science ◽  
2021 ◽  
pp. 108696
Author(s):  
Van-Ba Hoa ◽  
Dong-Heon Song ◽  
Kuk-Hwan Seol ◽  
Sun-Moon Kang ◽  
Hyun-Wook Kim ◽  
...  
Keyword(s):  
Shelf Life ◽  
Lauric Acid ◽  
Refrigerated Storage ◽  
Beef Steaks ◽  
Packaged Beef

scholarly journals Evaluation of Recommended Water Sample Collection Methods and the Impact of Holding Time on Legionella Recovery and Variability from Healthcare Building Water Systems

2020 ◽  
Vol 8 (11) ◽  
pp. 1770
Author(s):  
Marisa B. Hirsh ◽  
Julianne L. Baron ◽  
Sue M. Mietzner ◽  
John D. Rihs ◽  
Mohamed H. Yassin ◽  
...  
Keyword(s):  
Hold Time ◽  
Field Measurements ◽  
Quality Parameters ◽  
Hot Water ◽  
Control Measures ◽  
Sampling Location ◽  
Sample Collection ◽  
Water Safety ◽  
Processing Methods ◽  
The Impact

Water safety and management programs (WSMP) utilize field measurements to evaluate control limits and monitor water quality parameters including Legionella presence. This monitoring is important to verify that the plan is being implemented properly. However, once it has been determined when and how to sample for Legionella, it is important to choose appropriate collection and processing methods. We sought to compare processing immediate and flushed samples, filtration of different volumes collected, and sample hold times. Hot water samples were collected immediately and after a 2-min flush. These samples were plated directly and after filtration of either 100 mL, 200 mL, or 1 L. Additionally, unflushed samples were collected and processed immediately and after 1, 24, and 48 h of hold time. We found that flushed samples had significant reductions in Legionella counts compared to immediate samples. Processing 100 mL of that immediate sample both directly and after filter concentration yielded the highest concentration and percent sample positivity, respectively. We also show that there was no difference in culture values from time 0 compared to hold times of 1 h and 24 h. At 48 h, there were slightly fewer Legionella recovered than at time 0. However, Legionella counts were so variable based on sampling location and date that this hold time effect was minimal. The interpretation of Legionella culture results depends on the sample collection and processing methods used, as these can have a huge impact on the success of sampling and the validation of control measures.

Impact of Sampling Area and Location on Measurement of Indicator Organisms during Beef Carcass Interventions†

2013 ◽  
Vol 76 (12) ◽  
pp. 2069-2073 ◽  
Author(s):  
RONG WANG ◽  
DAVID A. KING ◽  
MOHAMMAD KOOHMARAIE ◽  
JOSEPH M. BOSILEVAC
Keyword(s):  
Sampling Method ◽  
Sampling Site ◽  
Hot Water ◽  
The Other ◽  
Indicator Organisms ◽  
Sample Collection ◽  
Beef Processing ◽  
Processing Plants ◽  
Multiple Indicator ◽  
Beef Carcass

The effect of the sponge sample collection site on the recovery of multiple indicator organisms from beef carcass surfaces was evaluated to simplify and validate our previous sampling method for ease of implementation as a general protocol. Sponge samples were collected at three beef processing plants using hot water or acidic antimicrobials as interventions. Two 4,000-cm2 samples were collected from preevisceration carcasses (n = 248), one from the inside and outside round area (top site) and one from the navel-plate-brisket-foreshank area (bottom site). One-half of the samples (n = 124) were collected before a wash cabinet intervention and the other half after the intervention. The numbers of total aerobic bacteria, Enterobacteriaceae, coliforms, and Escherichia coli were determined for one-half of each individual sponge sample. The other halves of the sponges were combined to represent a top plus bottom 8,000-cm2 sample. For the preintervention carcasses, 4,000-cm2 samples collected from the top or bottom sites of the carcasses were not significantly different (P &gt; 0.05) from each other or from the 8,000-cm2 combined sample in recovery of the indicator organisms. Significant reductions of indicator organisms were observed in all three types of sponge samples after intervention; however, samples collected from the bottom site recovered less organisms (P &lt; 0.05) compared with samples of the other types. These results suggested that samples collected from either the top or the bottom site of the carcasses with this method are suitable for monitoring indicator organisms as long as the same sampling site is consistently used.

scholarly journals Impact of the COVID-19 pandemic and New Normal implementation on credit risk and profitability of Indonesian banking institutions

2021 ◽  
Vol 16 (3) ◽  
pp. 104-112
Author(s):  
Sri Wahyuni ◽  
Pujiharto ◽  
Siti Nur Azizah ◽  
Zulfikar Zulfikar
Keyword(s):  
Credit Risk ◽  
Financial Services ◽  
Stock Exchange ◽  
Banking System ◽  
Secondary Data ◽  
Sample Collection ◽  
New Normal ◽  
Significant Difference ◽  
Before And After ◽  
The Impact

This study aims to compare the credit risk and profitability of banks in Indonesia. For this, the descriptive-quantitative method is used. The sample collection is based on the purposive sampling method. The study involved 71 Indonesian banks listed on the Indonesian Stock Exchange and Financial Services Authority, both conventional and Sharia. The research data are secondary data that include published results of quarterly financial reports of both conventional and sharia banks obtained from the website of the Financial Services Authority or the official websites of banks. The profitability of banks in making profit is measured by the Return on Assets ratio. The method of analysis used is the paired sample t-test. The results show significant differences in nonperforming loans (NPL) before and after the COVID-19 pandemic in conventional banking. However, there is no significant difference in Sharia banking. Moreover, there is no significant difference in profitability before and after the new normal implementation. This study provides empirical evidence that Indonesia’s banking restructuring policies to anticipate the impact of COVID-19 did not work optimally. The study is expected to help bank managers and the Financial Services Authority as a basis for evaluating the implementation of government policies to restructure the banking system.

Usefulness of a New Polymer Sampling Tube for Coagulation Testing in Untreated Patients and in Patients on Traditional Anticoagulant Therapy. Results of a Multicentre Evaluation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3974-3974 ◽  
Author(s):  
Pierre A. Toulon ◽  
Marie-Francoise Aillaud ◽  
Dominique Arnoux ◽  
Benedicte Delahousse ◽  
Celine Gourmel
Keyword(s):  
Shelf Life ◽  
Coagulation System ◽  
Blood Collection ◽  
Anticoagulant Treatment ◽  
Citrate Concentration ◽  
Coagulation Testing ◽  
Significant Difference ◽  
Glass Tubes ◽  
The Impact ◽  
Polymer Tubes

Abstract For years, laboratories have used evacuated glass tubes for blood collection. To improve the safety of blood collection, plastic tubes have been developed but various interactions with the coagulation system and/or antithrombotic drugs were reported with the first generation of such tubes. The aim of this multicentre study was to compare the results of different coagulation parameters measured in evacuated polymer (polyethylene terephtalate outer layer and polypropylene inner layer) tubes (Vacuette®, Greiner Bio-One) containing two different citrate concentrations (0.109 M and 0.129 M) with those measured in evacuated siliconized glass tubes (Vacutainer®, Becton-Dickinson) containing the same citrate concentrations. In addition, the impact of aging of the polymer tube was investigated by collecting blood samples in tubes at 11 months (beginning of shelf life=BSL) and at 1 month (end of shelf life=ESF) from expiry (only evaluated using the 0.109 M citrate concentration). A single batch of the different tubes was used in the present study. For that purpose, blood was drawn in the 5 participating centers from untreated patients (n=60), patients on oral anticoagulant treatment (OAT, n=168), and patients treated with either unfractionated heparin (UFH, n=123) or a low molecular weight derivative (LMWH, n=106). The sampling order of the different tubes was randomly defined. Prothrombin time (PT) or INR, activated partial thromboplastin time (APTT), factor V as well as anti-Xa activity (when applicable) were performed in each center using its own routine techniques, immediately after centrifugation. Coagulation activation markers i.e. thrombin-antithrombin complexes (TAT) and prothrombin fragment 1+2 (F1+2) were analyzed in a subset of untreated patients (n=30) after centralization in a single center. In addition, the stability of the samples was evaluated for the routine tests over a 6-hour period in untreated patients. The results of the coagulation tests were significantly different in polymer and glass tubes, from an analytical point of view (paired Student’s T-test). However, the difference was without any clinical relevance (Bland-Altman analysis), whatever the citrate concentration and the patients group. Furthermore, there was no significant difference in the results obtained in BSL and ESL polymer tubes. Interestingly, the plasma levels of TAT and F1+2 were significantly lower when blood was drawn in the polymer tubes for both citrate concentrations, even if the markers remained within the expected range in all cases. A significant evolution of the results, comparable for all types of tubes, was demonstrated for PT, APTT and FV over the 6-hour period. These results suggest that samples collected into the Vacuette® polymer tubes allow accurate routine coagulation testing both in untreated patients and in patients on traditional anticoagulant treatment during the whole shelf life indicated by the manufacturer. In addition, the observation of a lower activation of the coagulation system in the polymer tubes than in the glass tubes, containing a similar citrate concentration, deserves to be further investigated.

scholarly journals Bacteria from the Bacillus cereus group as contaminants in requeijão curd cheeses and especialidade láctea tipo requeijão

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Jamile de Oliveira Hachiya ◽  
Gabriel Augusto Marques Rossi ◽  
Higor Oliveira Silva ◽  
Rafael Akira Sato ◽  
Ana Maria Centola Vidal ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Shelf Life ◽  
Dairy Products ◽  
Bacterial Group ◽  
Bacillus Cereus Group ◽  
Significant Difference ◽  
The Impact

ABSTRACT: This study aimed to evaluate the occurrence of Bacillus cereus group in requeijões and especialidades lácteas tipo requeijão (regular and light) and to verify if there is differences in relation to this occurrence among different categories of these products. A set of 14 (35%) lots was contaminated with this bacterial group from the 40 lots with low counts (maximum 3.1 × 10 CFU/g), and no significant difference regarding counts or presence/absence were observed among the categories of the products. It can be concluded that contamination by B. cereus group in these products is unable to consist in risk to consumers, regarding adequate refrigeration during selling. This study was the first one to report this bacteria group for these dairy products and highlights the needs of further investigations to evaluate the impact of its spoilage during shelf life.

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