The Influence of Milk Type on the Proteolysis and Antioxidant Capacity of White-Brined Cheese Manufactured from High-Heat-Treated Milk Pretreated with Chymosin

We investigated the effect of milk type on the proteolysis and total antioxidant capacity (TEAC) of white-brined cheeses prepared from high-heat-treated (90 °C, 10 min) cow’s and goat’s milk, pretreated with chymosin at a low temperature (4 °C). The cheeses produced showed improved antioxidant characteristics and a high content of denatured whey proteins. However, these characteristics depend on the type of milk and the ripening time. Ripened cow’s milk cheese had higher values of WSN/TN (water-soluble nitrogen per total nitrogen content) and TCA-SN/TN (nitrogen soluble in 12% trichloroacetic acid per total nitrogen), but similar PTA-SN/TN (nitrogen soluble in 5% phosphotungstic acid per total nitrogen) values were observed in ripened cheeses. The antioxidant potential of a WSF (water-soluble fraction) was higher in goat’s milk cheese, but higher TEAC (total antioxidant capacity) values of WINF (water-insoluble fraction) were observed in matured cow’s milk cheese. In vitro digestion slightly improved the radical scavenging capacity of WSF, whereas digested WINF had more than twice the capacity of their undigested counterparts. The cheeses prepared in this study could be a good source of antioxidant peptides. Further investigations of their in vitro and in vivo functionality need to be conducted.

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The effect of in vitro digestion on antioxidant properties of water-soluble and insoluble protein fractions of traditional Serbian white- brined cheeses

Linear vs piecewise Weibull model for genetic evaluation of sires for longevity in Simmental cattle ◽

10.15567/mljekarstvo.2020.0403 ◽

2020 ◽

Vol 70 (4) ◽

pp. 253-265

Author(s):

Tanja Vučić ◽

Danijel Milinčić ◽

Miroljub Barać ◽

Aleksandar Kostić ◽

Olivera Ećim-Đurić ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Properties ◽

Reducing Power ◽

In Vitro Digestion ◽

Water Soluble ◽

Protein Fractions ◽

Antioxidant Capacities ◽

Total Antioxidant

The influence of simulated in vitro digestion on antioxidant potential of protein fractions of traditional Serbian white-brined cheeses was investigated. Water-soluble (WSF) and water-insoluble fractions (WINF) of three cow’s and three ovine white-brined cheeses were fractionated. Total antioxidant capacity, reducing power and iron (II) chelating properties of these fractions before and after in vitro digestion were assayed. The investigated protein fractions had different antioxidant properties. WSFs had a better total antioxidant capacity and reducing power and less pronounced iron (II) chelating properties than WINFs. A strong negative correlation (-0.818, P<0.05) between the total antioxidant capacities of undigested WSF and WINF of traditional cheeses were observed. In vitro digestion greatly improved the total antioxidant capacities of WINFs (by 16.61-34.18 times), their reducing power (up to 95.77 %) and except in the case of Svrljig ovine cheese, the iron (II) chelating ability as well. A less pronounced increase (up to 71.29 %) of the total antioxidant capacity of WSFs was induced by in vitro digestion. In vitro digestion reduced reducing power of WSF of investigated ovine cheeses as well as reducing power of WSF of Homolje cow’s cheese. Since there was no significant correlation between the investigated antioxidant properties of digested WSFs and their free amino acids and mineral contents the observed differences should be attributed to different composition and properties of low molecular weight peptides. Thus, further investigations related to their isolation and characterization needs to be conducted. However, these results indicate that Serbian white-brined cheeses have great potential as source of antioxidant peptides.

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The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses

Foods ◽

10.3390/foods8030094 ◽

2019 ◽

Vol 8 (3) ◽

pp. 94 ◽

Cited By ~ 5

Author(s):

Miroljub Barac ◽

Tanja Vucic ◽

Sladjana Zilic ◽

Mirjana Pesic ◽

Marina Sokovic ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Properties ◽

Reducing Power ◽

In Vitro Digestion ◽

Water Soluble ◽

Antifungal Potential ◽

Total Antioxidant ◽

Ace Inhibitory

This study deals with the effect of in vitro digestion on the functional potential of traditional Serbian white-brined cheeses. The total antioxidant capacity, reducing power and iron (II) chelating properties as well as angiotensin-converting enyzme-inhibitory (ACE-inhibitory) and antimicrobial activities of traditional Serbian white-brined cheeses before and after in vitro digestion were assayed. The traditional cheeses had different antioxidant properties as well as different ACE-inhibitory activities. In vitro digestion improved the total antioxidant capacity (8.42–58.56 times) and the reducing power (by 17.90–99.30%) of investigated cheeses, whereas their chelating ability was slightly improved or unaffected after digestion. In vitro digestion reduced the ACE-inhibitory potential of water-soluble protein fractions, and digested water-insoluble fractions were the major source of ACE-inhibitory peptides. The digestates did not exhibit any antibacterial potential, whereas they showed moderate antifungal potential toward selected micromycetes. The best antifungal potential had Svrljig ovine cheese and Homolje cow cheese. The results of this study clearly point to a significant functionality of traditional white-brined cheeses.

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Stability of the total antioxidant capacity of fresh fruit juices subjected to an in vitro digestion procedure

Proceedings of The Nutrition Society ◽

10.1017/s0029665110003757 ◽

2010 ◽

Vol 69 (OCE6) ◽

Author(s):

L. Ryan ◽

S. Prescott

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

In Vitro Digestion ◽

Fruit Juices ◽

Fresh Fruit ◽

Digestion Procedure ◽

Total Antioxidant

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Acute Effects Of Heat-treated Cherry Consumption On Serum Total Antioxidant Capacity

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000762384.84036.f5 ◽

2021 ◽

Vol 53 (8S) ◽

pp. 282-283

Author(s):

Greggory R. Davis ◽

David Bellar

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Acute Effects ◽

Heat Treated ◽

Total Antioxidant

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Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/639868 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Martina Horvathova ◽

Zuzana Orszaghova ◽

Lucia Laubertova ◽

Magdalena Vavakova ◽

Peter Sabaka ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Capacity ◽

Healthy Volunteers ◽

Total Antioxidant Capacity ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Antioxidant ◽

Trolox Equivalent ◽

Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

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Role of total antioxidant capacity in the differential growth of human embryos in vitro

Fertility and Sterility ◽

10.1016/j.fertnstert.2006.01.025 ◽

2006 ◽

Vol 86 (2) ◽

pp. 304-309 ◽

Cited By ~ 16

Author(s):

Mohamed Bedaiwy ◽

Ashok Agarwal ◽

Tamer M. Said ◽

Jeffery M. Goldberg ◽

Rakesh K. Sharma ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Human Embryos ◽

Differential Growth ◽

Total Antioxidant

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Estimate of antioxidant capacity and lipid peroxidation in plasma of healthy subjects

Open Medicine ◽

10.2478/s11536-006-0008-9 ◽

2006 ◽

Vol 1 (1) ◽

pp. 23-34

Author(s):

Anna Gorąca ◽

Beata Skibska

Keyword(s):

Ascorbic Acid ◽

Uric Acid ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Plasma Concentrations ◽

Beta Carotene ◽

Alpha Tocopherol ◽

Elderly Subjects ◽

Total Antioxidant

AbstractSerum contains various antioxidant molecules that may provide important protection against free radical attack. The aim of this work was to assess the total antioxidant capacity of plasma and a marker of lipid per oxidation [(thiobarbituric acid-reactive substances (TBARS)] in plasma of healthy smoking and non-smoking young and elderly subjects. In addition, we investigated plasma concentrations of α-tocopherol, β-carotene, and ascorbic acid. In in vitro experiments, the effects of exogenous compounds (ascorbic acid, uric acid, Trolox) on total ferric-reducing activity of plasma (FRAP) were also tested. We demonstrated that total antioxidant capacity of plasma obtained from healthy non-smoking young subjects was significantly higher than plasma antioxidant capacity of smoking elderly subjects. The concentration of TBARS in young non-smoking volunteers was lower than that in young smokers. The concentration of TBARS in elderly non-smoking volunteers was lower than in elderly smokers. Plasma concentrations of alpha-tocopherol, beta-carotene and ascorbic acid were significantly lower in elderly smoker than in elderly non-smokers of the same age. No difference in the plasma levels of alpha-tocopherol, beta-carotene and ascorbic acid were found in 22-year-old smoking and non-smoking subjects. In vitro addition of ascorbic acid, uric acid, or Trolox to plasma samples significantly increased their total antioxidant capacity. Decrease of FRAP values and increase of TBARS concentrations is a significant physiologic condition of the aging process. Supplementation of antioxidants could be useful for the enhancement of antioxidant screen in plasma.

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ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF RUBUS FRUTICOSUS AND ZIZYPHUS VULGARIS METHANOL EXTRACTS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.14374 ◽

2017 ◽

Vol 9 (2) ◽

pp. 69

Author(s):

Asma Meziti ◽

Hamama Bouriche ◽

Meziti Hichem ◽

Seoussen Kada ◽

Abderrahmane Senator ◽

...

Keyword(s):

Oral Administration ◽

Antioxidant Capacity ◽

Vascular Permeability ◽

Dna Cleavage ◽

Total Antioxidant Capacity ◽

Rubus Fruticosus ◽

Total Antioxidant ◽

Anti Inflammatory

Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo.Methods: Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability.Results: The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.Conclusion: The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents.

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