Stability of the total antioxidant capacity of fresh fruit juices subjected to an in vitro digestion procedure

The influence of simulated in vitro digestion on antioxidant potential of protein fractions of traditional Serbian white-brined cheeses was investigated. Water-soluble (WSF) and water-insoluble fractions (WINF) of three cow’s and three ovine white-brined cheeses were fractionated. Total antioxidant capacity, reducing power and iron (II) chelating properties of these fractions before and after in vitro digestion were assayed. The investigated protein fractions had different antioxidant properties. WSFs had a better total antioxidant capacity and reducing power and less pronounced iron (II) chelating properties than WINFs. A strong negative correlation (-0.818, P<0.05) between the total antioxidant capacities of undigested WSF and WINF of traditional cheeses were observed. In vitro digestion greatly improved the total antioxidant capacities of WINFs (by 16.61-34.18 times), their reducing power (up to 95.77 %) and except in the case of Svrljig ovine cheese, the iron (II) chelating ability as well. A less pronounced increase (up to 71.29 %) of the total antioxidant capacity of WSFs was induced by in vitro digestion. In vitro digestion reduced reducing power of WSF of investigated ovine cheeses as well as reducing power of WSF of Homolje cow’s cheese. Since there was no significant correlation between the investigated antioxidant properties of digested WSFs and their free amino acids and mineral contents the observed differences should be attributed to different composition and properties of low molecular weight peptides. Thus, further investigations related to their isolation and characterization needs to be conducted. However, these results indicate that Serbian white-brined cheeses have great potential as source of antioxidant peptides.

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The Effect of In Vitro Digestion on Antioxidant, ACE-Inhibitory and Antimicrobial Potentials of Traditional Serbian White-Brined Cheeses

Foods ◽

10.3390/foods8030094 ◽

2019 ◽

Vol 8 (3) ◽

pp. 94 ◽

Cited By ~ 5

Author(s):

Miroljub Barac ◽

Tanja Vucic ◽

Sladjana Zilic ◽

Mirjana Pesic ◽

Marina Sokovic ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Properties ◽

Reducing Power ◽

In Vitro Digestion ◽

Water Soluble ◽

Antifungal Potential ◽

Total Antioxidant ◽

Ace Inhibitory

This study deals with the effect of in vitro digestion on the functional potential of traditional Serbian white-brined cheeses. The total antioxidant capacity, reducing power and iron (II) chelating properties as well as angiotensin-converting enyzme-inhibitory (ACE-inhibitory) and antimicrobial activities of traditional Serbian white-brined cheeses before and after in vitro digestion were assayed. The traditional cheeses had different antioxidant properties as well as different ACE-inhibitory activities. In vitro digestion improved the total antioxidant capacity (8.42–58.56 times) and the reducing power (by 17.90–99.30%) of investigated cheeses, whereas their chelating ability was slightly improved or unaffected after digestion. In vitro digestion reduced the ACE-inhibitory potential of water-soluble protein fractions, and digested water-insoluble fractions were the major source of ACE-inhibitory peptides. The digestates did not exhibit any antibacterial potential, whereas they showed moderate antifungal potential toward selected micromycetes. The best antifungal potential had Svrljig ovine cheese and Homolje cow cheese. The results of this study clearly point to a significant functionality of traditional white-brined cheeses.

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Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/639868 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Martina Horvathova ◽

Zuzana Orszaghova ◽

Lucia Laubertova ◽

Magdalena Vavakova ◽

Peter Sabaka ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Capacity ◽

Healthy Volunteers ◽

Total Antioxidant Capacity ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Antioxidant ◽

Trolox Equivalent ◽

Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

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Role of total antioxidant capacity in the differential growth of human embryos in vitro

Fertility and Sterility ◽

10.1016/j.fertnstert.2006.01.025 ◽

2006 ◽

Vol 86 (2) ◽

pp. 304-309 ◽

Cited By ~ 16

Author(s):

Mohamed Bedaiwy ◽

Ashok Agarwal ◽

Tamer M. Said ◽

Jeffery M. Goldberg ◽

Rakesh K. Sharma ◽

...

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Human Embryos ◽

Differential Growth ◽

Total Antioxidant

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Total Antioxidant Capacity and Total Phenol Contents of Selected Commercial Fruit Juices in Turkey

International Journal of Food Properties ◽

10.1080/10942912.2010.499039 ◽

2010 ◽

Vol 13 (6) ◽

pp. 1373-1379 ◽

Cited By ~ 24

Author(s):

Emine Nur Herken ◽

Salih Guzel

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Fruit Juices ◽

Total Phenol ◽

Total Antioxidant

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Prediction of Total Antioxidant Capacity of Fruit Juices Using FTIR Spectroscopy and PLS Regression

Food Analytical Methods ◽

10.1007/s12161-011-9251-z ◽

2011 ◽

Vol 5 (3) ◽

pp. 405-407 ◽

Cited By ~ 8

Author(s):

Loredana Florina Leopold ◽

Nicolae Leopold ◽

Horst A. Diehl ◽

Carmen Socaciu

Keyword(s):

Antioxidant Capacity ◽

Ftir Spectroscopy ◽

Total Antioxidant Capacity ◽

Fruit Juices ◽

Pls Regression ◽

Total Antioxidant

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Estimate of antioxidant capacity and lipid peroxidation in plasma of healthy subjects

Open Medicine ◽

10.2478/s11536-006-0008-9 ◽

2006 ◽

Vol 1 (1) ◽

pp. 23-34

Author(s):

Anna Gorąca ◽

Beata Skibska

Keyword(s):

Ascorbic Acid ◽

Uric Acid ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Plasma Concentrations ◽

Beta Carotene ◽

Alpha Tocopherol ◽

Elderly Subjects ◽

Total Antioxidant

AbstractSerum contains various antioxidant molecules that may provide important protection against free radical attack. The aim of this work was to assess the total antioxidant capacity of plasma and a marker of lipid per oxidation [(thiobarbituric acid-reactive substances (TBARS)] in plasma of healthy smoking and non-smoking young and elderly subjects. In addition, we investigated plasma concentrations of α-tocopherol, β-carotene, and ascorbic acid. In in vitro experiments, the effects of exogenous compounds (ascorbic acid, uric acid, Trolox) on total ferric-reducing activity of plasma (FRAP) were also tested. We demonstrated that total antioxidant capacity of plasma obtained from healthy non-smoking young subjects was significantly higher than plasma antioxidant capacity of smoking elderly subjects. The concentration of TBARS in young non-smoking volunteers was lower than that in young smokers. The concentration of TBARS in elderly non-smoking volunteers was lower than in elderly smokers. Plasma concentrations of alpha-tocopherol, beta-carotene and ascorbic acid were significantly lower in elderly smoker than in elderly non-smokers of the same age. No difference in the plasma levels of alpha-tocopherol, beta-carotene and ascorbic acid were found in 22-year-old smoking and non-smoking subjects. In vitro addition of ascorbic acid, uric acid, or Trolox to plasma samples significantly increased their total antioxidant capacity. Decrease of FRAP values and increase of TBARS concentrations is a significant physiologic condition of the aging process. Supplementation of antioxidants could be useful for the enhancement of antioxidant screen in plasma.

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