Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar

Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways.

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Information technology. Digitally recorded media for information interchange and storage. Test method for the estimation of lifetime of optical disks for long-term data storage

10.3403/30341531u ◽

2017 ◽

Keyword(s):

Information Technology ◽

Data Storage ◽

Test Method ◽

Storage Test ◽

Optical Disks ◽

Information Interchange ◽

And Storage ◽

Term Data

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Information technology. Digitally recorded media for information interchange and storage. Test method for the estimation of lifetime of optical disks for long-term data storage

10.3403/30341531 ◽

2017 ◽

Keyword(s):

Information Technology ◽

Data Storage ◽

Test Method ◽

Storage Test ◽

Optical Disks ◽

Information Interchange ◽

And Storage ◽

Term Data

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MODERN PROBLEMATICS AND ANALYSIS OF EFFICIENCY OF APPLICATION OF DIFFERENT APPROACHES TO DESTRUCTION OF INFORMATION ON MAGNETIC MEDIA

Collection of scientific works of Odesa Military Academy ◽

10.37129/2313-7509.2019.12.2.154-164 ◽

2019 ◽

Vol 2 (12) ◽

pp. 154-164

Author(s):

O. Semenenko ◽

O. Vodchyts ◽

V. Koverga ◽

R. Lukash ◽

O. Lutsenko

Keyword(s):

Armed Forces ◽

Information Storage ◽

Storage Device ◽

Magnetic Media ◽

Long Term Storage ◽

Physical Impact ◽

And Storage ◽

Term Storage

The introduction and active use of information transmission and storage systems in the Ministry of Defense (MoD) of Ukraine form the need to develop ways of guaranteed removal of data from media after their use or long-term storage. Such a task is an essential component of the functioning of any information security system. The article analyzes the problems of guaranteed destruction of information on magnetic media. An overview of approaches to the guaranteed destruction of information on magnetic media of different types is presented, and partial estimates of the effectiveness of their application are given by some generally accepted indicators of performance evaluation. The article also describes the classification of methods of destruction of information depending on the influence on its medium. The results of the analysis revealed the main problems of application of software methods and methods of demagnetization of the information carrier. The issue of guaranteed destruction of information from modern SSD devices, which are actively used in the formation of new systems of information accumulation and processing, became particularly relevant in the article. In today's conditions of development of the Armed Forces of Ukraine, methods of mechanical and thermal destruction are more commonly used today. In the medium term, the vector of the use of information elimination methods will change towards the methods of physical impact by the pulsed magnetic field and the software methods that allow to store the information storage device, but this today requires specialists to develop new ways of protecting information in order to avoid its leakage.

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Long-Term Effects of Abuse in Later Life Perpetrated by Family Members

Innovation in Aging ◽

10.1093/geroni/igaa057.1438 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

pp. 444-445

Author(s):

Naomi Meinertz ◽

Pi-Ju Liu ◽

Ron Acierno

Keyword(s):

Social Support ◽

Depressive Symptoms ◽

Family Member ◽

Older Adult ◽

Family Members ◽

Later Life ◽

Adult Health ◽

Long Term Effects ◽

Long Term Impact

Abstract Abuse in later life could potentially lead to lower levels of social support, especially when perpetrated by family members who are charged with protecting the older adult in their care. Using both waves of the National Elder Mistreatment longitudinal data (wave one collected in 2008 and wave two in 2015; N=774), long-term effects of abuse (i.e., physical, emotional, sexual, and financial) on levels of social support, physical health, and clinical depressive symptoms for respondents at or above the age of 60 years were analyzed. A multivariate analysis of variance showed that respondents abused at wave one (n=261) by a family member (B=-0.55, p≤0.001), a spouse or ex-partner (B=-0.349, p=0.02), or a non-relative or stranger (B=-0.301, p=0.026) had lower levels of social support eight years later at wave two. Those abused by a family member at wave one also experienced higher levels of depressive symptoms at wave two (B=-0.187, p=0.01). Perpetrator type did not predict general health at wave two. These results emphasize the long-term impact of abuse on the lives of older adults and highlight the importance trusted relationships, such as with family members, have on older adult health and wellbeing.

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The Development and Testing of KNACC: Knowledgeable Nursing Assistants as Creative Caregivers

Innovation in Aging ◽

10.1093/geroni/igaa057.2346 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

pp. 675-675

Author(s):

Jacqueline Eaton ◽

Kristin Cloyes ◽

Brooke Paulsen ◽

Connie Madden ◽

Lee Ellington

Keyword(s):

Focus Groups ◽

Long Term Care ◽

Family Members ◽

Nursing Assistants ◽

Direct Care ◽

Term Care ◽

Memory Care

Abstract Nursing assistants (NAs) provide 80% of direct care in long-term care settings, yet are seldom viewed as skilled professionals. Empowering NAs is linked to improved resident outcomes. In this study, we collaborate with NAs to adapt and test the feasibility and acceptability of arts-based creative caregiving techniques (CCG) for use in long-term care. We held a series of focus groups (n=14) to adapt, refine, and enhance usability. We then evaluated implementation in two waves of testing (n=8). Those working in memory care units were more likely to use all techniques, while those working in rehabilitation were more hesitant to implement. Participants reported using CCG to distract upset residents. Family members were excited about implementation, and NAs not participating wanted to learn CCG. Nursing assistants have the potential to become experts in creative caregiving but may require in-depth training to improve use.

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Clinical characterization of 266 patients and family members with cleft lip and/or palate with associated malformations and syndromes

Clinical Oral Investigations ◽

10.1007/s00784-021-03863-2 ◽

2021 ◽

Author(s):

Theodosia Bartzela ◽

Björn Theuerkauf ◽

Elisabeth Reichardt ◽

Malte Spielmann ◽

Charlotte Opitz

Keyword(s):

Early Identification ◽

Congenital Malformations ◽

Cleft Lip ◽

Phenotypic Variability ◽

Family Members ◽

Pierre Robin Sequence ◽

Associated Malformations ◽

Pierre Robin

Abstract Objectives To clinically characterize patients and family members with cleft lip and/or palate (CL/P) and associated congenital malformations or syndromes and propose possible inheritance patterns. Materials and methods An observational study of patients with CL/P, including medical and family history and intra- and extra-oral examination of their family members, was performed. Results Two hundred sixty-six patients, 1257 family members, and 42 pedigrees were included in the study. The distribution of patients according to the cleft type was 57.9% with CLP, 25.2% with cleft palate (CPO), and 12.8% with cleft lip with/without alveolus (CL/A). Seventy-four (27.8%) patients had associated malformations, and 24 (9.2%) a syndrome. The skeletal (27.7%), cardiovascular (19.3%) systems, and eyes (22.9%) were most commonly affected. Pierre Robin Sequence (7 patients) and van der Woude (4) were the most common syndromes. The majority of patients with CPO (19/24) had an associate syndrome. The families had an average of 2.45 affected members. Conclusion Individual and interfamilial phenotypic variability in patients with CL/P makes the understanding of etiopathogenesis challenging. Clinical relevance The overall prevalence of individuals with CL/P and their pedigrees with associated malformations and syndromes emphasize the need for early identification, interdisciplinary, and long-term planning.

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Attitudes Toward Sexual Behaviors in Long-Term Care: Development of an Assessment Tool

Innovation in Aging ◽

10.1093/geroni/igaa057.2031 ◽

2020 ◽

Vol 4 (Supplement_1) ◽

pp. 602-603

Author(s):

Rachael Spalding ◽

Emma Katz ◽

Barry Edelstein

Keyword(s):

Sexual Behaviors ◽

Long Term Care ◽

Assessment Tool ◽

Qualitative Interviews ◽

Family Members ◽

Community Dwelling ◽

Term Care ◽

Care Development

Abstract Most older adults living in long-term care settings (LTCs) indicate that expressing their sexuality is important to them (Doll, 2013). Little is known about the general public’s attitudes towards sexual behaviors in LTCs. Attitudes of LTC residents’ family members are particularly important, as family members are most likely to visit residents and to care about their quality of life. Family members’ attitudes could in turn inform facility policies and management. We will present preliminary data from a series of qualitative interviews with community-dwelling adults regarding their attitudes. We will discuss how these data are being used to inform current work on a measure of attitudes toward sexual behavior in LTCs.

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Quality tracing in meat supply chains

Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rsta.2013.0308 ◽

2014 ◽

Vol 372 (2017) ◽

pp. 20130308 ◽

Cited By ~ 16

Author(s):

Miriam Mack ◽

Patrick Dittmer ◽

Marius Veigt ◽

Mehmet Kus ◽

Ulfert Nehmiz ◽

...

Keyword(s):

Supply Chains ◽

Process Analysis ◽

Decision Support Tool ◽

Temperature Monitoring ◽

Support Tool ◽

Logistics Network ◽

Primary Model ◽

Sensory Parameters ◽

And Storage

The aim of this study was the development of a quality tracing model for vacuum-packed lamb that is applicable in different meat supply chains. Based on the development of relevant sensory parameters, the predictive model was developed by combining a linear primary model and the Arrhenius model as the secondary model. Then a process analysis was conducted to define general requirements for the implementation of the temperature-based model into a meat supply chain. The required hardware and software for continuous temperature monitoring were developed in order to use the model under practical conditions. Further on a decision support tool was elaborated in order to use the model as an effective tool in combination with the temperature monitoring equipment for the improvement of quality and storage management within the meat logistics network. Over the long term, this overall procedure will support the reduction of food waste and will improve the resources efficiency of food production.

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