The Molecular Characteristics of the FAM13A Gene and the Role of Transcription Factors ACSL1 and ASCL2 in Its Core Promoter Region

The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle.

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The core promoter region of the tumor necrosis factor alpha gene confers phorbol ester responsiveness to upstream transcriptional activators.

Molecular and Cellular Biology ◽

10.1128/mcb.12.3.1352 ◽

1992 ◽

Vol 12 (3) ◽

pp. 1352-1356 ◽

Cited By ~ 29

Author(s):

D C Leitman ◽

E R Mackow ◽

T Williams ◽

J D Baxter ◽

B L West

Keyword(s):

Transcription Factors ◽

Tumor Necrosis Factor ◽

Promoter Region ◽

Tumor Necrosis ◽

Core Promoter ◽

Core Promoter Region ◽

Necrosis Factor Alpha ◽

The Core ◽

Factor Alpha ◽

Necrosis Factor

Activators of protein kinase C, such as 12-O-tetradecanoylphorbol 13-acetate (TPA), are known to regulate the expression of many genes, including the tumor necrosis factor alpha (TNF) gene, by affecting the level or activity of upstream transcription factors. To investigate the mechanism whereby TPA activates the TNF promoter, a series of 5'-deletion mutants of the human TNF promoter linked to chloramphenicol acetyltransferase was transfected into U937 human promonocytic cells. TPA produced a 7- to 11-fold activation of all TNF promoters tested, even those promoters truncated to contain only the core promoter with no upstream enhancer elements. The proximal TNF promoter containing only 28 nucleotides upstream and 10 nucleotides downstream of the RNA start site confers TPA activation to a variety of unrelated upstream enhancer elements and transcription factors, including Sp1, CTF/NF1, cyclic AMP-response element, GAL-E1a, and GAL-VP16. The level of activation by TPA depends on the TATA box structure, since the TPA response is greater in promoters containing the sequence TATAAA than in those containing TATTAA or TATTTA. These findings suggest that the core promoter region is a target for gene regulation by second-messenger pathways.

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Bioinformatics Analysis and Competitive Regulation by Transcription Factors of SIRT5 at the Core Promoter Region Using Bovine Adipocytes

DNA and Cell Biology ◽

10.1089/dna.2018.4385 ◽

2018 ◽

Vol 37 (12) ◽

pp. 1003-1015 ◽

Cited By ~ 1

Author(s):

Jieyun Hong ◽

Chugang Mei ◽

Xiaoyu Wang ◽

Gong Cheng ◽

Linsen Zan

Keyword(s):

Transcription Factors ◽

Promoter Region ◽

Bioinformatics Analysis ◽

Core Promoter ◽

Core Promoter Region ◽

The Core ◽

Bovine Adipocytes

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The core promoter region of the tumor necrosis factor alpha gene confers phorbol ester responsiveness to upstream transcriptional activators

Molecular and Cellular Biology ◽

10.1128/mcb.12.3.1352-1356.1992 ◽

1992 ◽

Vol 12 (3) ◽

pp. 1352-1356

Author(s):

D C Leitman ◽

E R Mackow ◽

T Williams ◽

J D Baxter ◽

B L West

Keyword(s):

Transcription Factors ◽

Tumor Necrosis Factor ◽

Promoter Region ◽

Tumor Necrosis ◽

Core Promoter ◽

Core Promoter Region ◽

Necrosis Factor Alpha ◽

The Core ◽

Factor Alpha ◽

Necrosis Factor

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Identification of the Transcription Factors of CD200R1 and Its Potential Roles in Parkinson’s Disease

10.21203/rs.3.rs-1064241/v1 ◽

2021 ◽

Author(s):

Suzhen Lin ◽

Ruinan Shen ◽

Hong Pan ◽

Lu He ◽

Fang Fang ◽

...

Keyword(s):

Transcription Factors ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Promoter Region ◽

Mononuclear Cells ◽

Core Promoter ◽

Core Promoter Region ◽

Peripheral Blood Mononuclear ◽

The Core ◽

Blood Mononuclear Cells

Abstract BackgroundNeuroinflammation is known to be involved in the pathogenesis of Parkinson's disease (PD). Abnormal activation of microglia plays a key role in this pathological process. CD200R1 is a membrane glycoprotein primarily expressed in microglia in central nervous system responsible for transducing signaling maintaining microglia in stationary status. Our previous studies have demonstrated the dysregulation of CD200R1 and its involvement in PD pathogenesis. The binding of transcription factors with promoter regions is the basic and essential step for the regulation of gene expression. However, little is known about the human CD200R1 promoter region and the mechanism of the dysregulated expression of CD200R1 in PD. MethodsLuciferase reporter system was initially employed to identify the core region of CD200R1 promoter and figure out its potential transcription factors. Subsequently, we investigated the interaction adopting electrophoretic mobility shift assay and chromatin immunoprecipitation assay. The regulatory function of the detected transcription factors were further proved through its down-regulation and overexpression. We then collected the peripheral blood mononuclear cells from both PD patients and their healthy counterparts with matched age and sex to evaluate whether consistent results existed under clinical setting. Ultimately, the mouse model was established through knocking-out the identified transcription factor and its role in neuroinflammation and pathogenesis of PD was explored. ResultsWe defined that the core promoter region of CD200R was located within -482 to -146 bp upstream of the translation initiation site (TIS). In addition, we demonstrated that NFKB1 directly bound to the CD200R1 core promoter region and regulated its transcriptional activity. Besides, the expression of NFKB1 and CD200R1 was significantly correlated in human peripheral blood mononuclear cells and knocking down NFKB1 significantly reduced CD200R1 expression. Moreover, both NFKB1 and CD200R1 were significantly downregulated in samples from PD patients. Furthermore, NFKB1-/- mice exhibited exacerbated microglia activation and dopaminergic neuron loss after MPTP treatment. ConclusionOur study provided novel understanding of the transcriptional regulation of CD200R1 and its role in microglia homeostasis in the pathogenesis of PD.

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Coordinate regulation by transcription factors and DNA methylation in the core promoter region of SIRT6 in bovine adipocytes

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2018.09.018 ◽

2018 ◽

Vol 659 ◽

pp. 1-12 ◽

Cited By ~ 5

Author(s):

Jie-yun Hong ◽

Chu-gang Mei ◽

Shi-jun Li ◽

Hong-bao Wang ◽

Chun-ping Zhao ◽

...

Keyword(s):

Dna Methylation ◽

Transcription Factors ◽

Promoter Region ◽

Core Promoter ◽

Core Promoter Region ◽

Coordinate Regulation ◽

The Core ◽

Bovine Adipocytes

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The −5 A/G single-nucleotide polymorphism in the core promoter region of MT2A and its effect on allele-specific gene expression and Cd, Zn and Cu levels in laryngeal cancer

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2014.08.016 ◽

2014 ◽

Vol 280 (2) ◽

pp. 256-263 ◽

Cited By ~ 7

Author(s):

Katarzyna Starska ◽

Anna Krześlak ◽

Ewa Forma ◽

Jurek Olszewski ◽

Alina Morawiec-Sztandera ◽

...

Keyword(s):

Gene Expression ◽

Single Nucleotide Polymorphism ◽

Promoter Region ◽

Core Promoter ◽

Specific Gene ◽

Nucleotide Polymorphism ◽

Core Promoter Region ◽

Single Nucleotide ◽

The Core ◽

Allele Specific

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High Frequency of Either Altered Pre-Core Start Codon or Weakened Kozak Sequence in the Core Promoter Region in Hepatitis B Virus A1 Strains from Rwanda

Genes ◽

10.3390/genes10030182 ◽

2019 ◽

Vol 10 (3) ◽

pp. 182 ◽

Cited By ~ 1

Author(s):

Heléne Norder ◽

Theogene Twagirumugabe ◽

Joanna Said ◽

Yarong Tian ◽

Ka-Wei Tang ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Promoter Region ◽

Core Promoter ◽

Etiologic Agent ◽

Start Codon ◽

Core Promoter Region ◽

Kozak Sequence ◽

The Core ◽

B Virus

Hepatitis B virus (HBV) is endemic in Rwanda and is a major etiologic agent for chronic liver disease in the country. In a previous analysis of HBV strains from Rwanda, the S genes of most strains segregated into one single clade of subgenotype, A1. More than half (55%) of the anti-HBe positive individuals were viremic. In this study, 23 complete HBV genomes and the core promoter region (CP) from 18 additional strains were sequenced. Phylogenetic analysis of complete genomes confirmed that most Rwandan strain formed a single unique clade, within subgenotype A1. Strains from 17 of 22 (77%) anti-HBe positive HBV carriers had either mutated the precore start codon (9 strains with either CUG, ACG, UUG, or AAG) or mutations in the Kozak sequence preceding the pre-core start codon (8 strains). These mutually exclusive mutations were also identified in subgenotypes A1 (70/266; 26%), A2 (12/255; 5%), and A3 (26/49; 53%) sequences from the GenBank. The results showed that previous, rarely described HBV variants, expressing little or no HBeAg, are selected in anti-HBe positive subgenotype Al carriers from Rwanda and that mutations reducing HBeAg synthesis might be unique for a particular HBV clade, not just for a specific genotype or subgenotype.

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