scholarly journals Coniferous Cones as a Forestry Waste Biomass—A Source of Antioxidants

2020 ◽  
Vol 3 (1) ◽  
pp. 82
Author(s):  
Tamás Hofmann ◽  
Levente Albert ◽  
Balázs Bocz ◽  
Dániel Bocz ◽  
Eszter Visi-Rajczi
Keyword(s):  
Picea Abies ◽  
Cryptomeria Japonica ◽  
Tsuga Canadensis ◽  
Total Phenol Content ◽  
Waste Biomass ◽  
Metasequoia Glyptostroboides ◽  
Antioxidant Power ◽  
Chamaecyparis Lawsoniana ◽  
Tandem Mass Spectrometric ◽  
Thuja Orientalis

The cones of conifers are a waste biomass, potentially be utilized for a variety of purposes, including the extraction of bioactive materials, particularly antioxidant polyphenols. In the present work we conducted a comparative analysis of the antioxidant content of selected taxa that are either common in Hungary or that have not yet been investigated in any great detail (Cedrus atlantica, Larix decidua, Picea abies, Pinus mugo, Pinus nigra, Pinus sylvestris, Pinus wallichiana, Tsuga Canadensis, Tsuga heterophylla, Pseudotsuga menziesii, Chamaecyparis lawsoniana, Taxodium distichum, Thuja occidentalis, Metasequoia glyptostroboides, Thuja orientalis, Cryptomeria Japonica, Cunninghamia lanceolata). A comparison of different maturation stages (green, mature, and opened cones) was carried out for the assigned taxa. Folin-Ciocâlteu total phenol content, ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to assess the antioxidant contents. Total antioxidant power was determined by a scoring system that combined the three assay results. For each taxon the overall best results were found for green cones, followed by mature, and opened cones. Taxa with the highest scores were Tsuga Canadensis, Metasequoia glyptostroboides, Chamaecyparis lawsoniana, Cryptomeria Japonica, Thuja orientalis and Picea abies. High-performance liquid chromatographic/tandem mass spectrometric profiling of the polyphenols was completed for selected samples. Results provide a basis for future bioactivity testing of these samples. The research was supported by the ÚNKP-20-5-12 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund and by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences.

scholarly journals Antioxidant Capacity and Tentative Identification of Polyphenolic Compounds of Cones of Selected Coniferous Species

2020 ◽  
Vol 16 (2) ◽  
pp. 79-94
Author(s):  
Tamás Hofmann ◽  
Eszter Visi-Rajczi ◽  
Balázs Bocz ◽  
Dániel Bocz ◽  
Levente Albert
Keyword(s):  
Picea Abies ◽  
Cryptomeria Japonica ◽  
Scientific Literature ◽  
Tsuga Canadensis ◽  
Metasequoia Glyptostroboides ◽  
Antioxidant Power ◽  
Coniferous Species ◽  
Antioxidant Content ◽  
Chamaecyparis Lawsoniana ◽  
Thuja Orientalis

The cones of coniferous species are a waste biomass byproduct that can be potentially utilized for a variety of purposes. One of the many application fields is the extraction of bioactive materials, particularly antioxidant polyphenols. Scientific literature on the antioxidant content of coniferous cones at different ripening stages is limited. In this study, we conducted a comparative analysis of the antioxidant content of selected taxa that are either common in Hungary or that have not yet been investigated in the scientific literature in any great detail (Cedrus atlantica, Larix decidua, Picea abies, Pinus mugo, Pinus nigra, Pinus sylvestris, Pinus wallichiana, Tsuga canadensis, Tsuga heterophylla, Chamaecyparis lawsoniana, Taxodium distichum, Thuja occidentalis, Metasequoia glyptostroboides, Thuja orientalis, Cryptomeria japonica, Cunninghamia lanceolata). A comparison of green, mature and opened cones was performed for the assigned taxa. Folin-Ciocâlteu total polyphenol content (TPC), ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to assess the antioxidant contents. Overall antioxidant power was determined by a scoring system that combined the three assay results. In general, best values were found for green cones, followed by mature, and opened cones for each taxon. Tsuga canadensis, Metasequoia glyptostroboides, Chamaecyparis lawsoniana, Cryptomeria japonica, Thuja orientalis and Picea abies all contained high amounts of antioxidants in both green and mature cones and attained the highest scores. High-performance liquid chromatographic/tandem mass spectrometric profiling of the cone polyphenols was also completed for selected samples. Results provide a basis for future bioactivity testing of these samples.

scholarly journals Cones of coniferous taxa as a potential source of bioactive polyphenols

2021 ◽  
Vol 18 ◽  
Author(s):  
Tamás Hofmann ◽  
Levente Albert ◽  
Balázs Bocz ◽  
Dániel Bocz ◽  
Eszter Visi-Rajczi
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Chamaecyparis Lawsoniana ◽  
Tandem Mass Spectrometric ◽  
Thuja Orientalis ◽  
Liquid Chromatographic

Background: Coniferous cones are a by-product of forestry and wood logging, used for many possible purposes, e.g., the extraction of polyphenols. Objective: The aim of the present article was the comparison of the antioxidant polyphenol content of the differently matured cones of 17 selected conifers, either common in Hungary or yet uninvestigated. Methods: Total polyphenol content, ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl assays were used to determine the antioxidant contents. A scoring system was implemented using the three assay results to evaluate and compare the overall antioxidant power of the samples. Result and Conclusion: Highest antioxidant contents were found in green cones, followed by mature and opened cones. Taxa with the highest scores were Tsuga canadensis, Cryptomeria japonica, Chamaecyparis lawsoniana, Thuja orientalis, Metasequoia glyptostroboides and Picea abies. For the samples with the highest overall antioxidant power the high-performance liquid chromatographic/tandem mass spectrometric polyphenol profiling was carried out (green cones of T. canadensis and P. abies) and 83 compounds have been tentatively identified and described. Results contribute to the future bioactivity testing and evaluation of the cone extracts of T. canadensis and P. abies.

scholarly journals Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1191
Author(s):  
Mahak Majeed ◽  
Tanveer Bilal Pirzadah ◽  
Manzoor Ahmad Mir ◽  
Khalid Rehman Hakeem ◽  
Hesham F. Alharby ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Juglans Regia ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Viscum Album ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

Antioxidant Properties and Polyphenol Screening of the Leaves of Native Hungarian Oak (Quercus) Species

2021 ◽  
Vol 17 ◽  
Author(s):  
Tamás Hofmann ◽  
Eszter Visi-Rajczi ◽  
Levente Albert
Keyword(s):  
Antioxidant Capacity ◽  
State Of The Art ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Flavonoid Glycosides ◽  
Waste Biomass ◽  
Quercus Species ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic ◽  
Phenolic Acid Derivatives

Background: Due to their ecological significance and timber value, Quercus species are especially important in Hungary. Nevertheless, the leaves of these species lack a dedicated utilization field and are considered a waste biomass. Materials and Methods: The present study comprehensively analyses three selected oak species (Q. petraea, Q. pubescens, Q. cerris) native to Hungary to assess their antioxidant capacity (FRAP, ABTS, DPPH) and provide information on their polyphenol pool using state-of-the-art liquid chromatographic/tandem mass spectrometric technique. To the best of our knowledge, no such investigation has yet been conducted for the assigned species. Results: According to the results, the antioxidant capacity of the three species’ leaves are roughly equal. Altogether, 109 compounds have been tentatively identified and described, including phenolic acid derivatives, tannins, flavonoid glycosides, and catechins. Compared to other oak leaf samples and other types of plant tissues, the investigated samples contained a large number (24) of acylated polyphenols. Conclusion: The recent findings on the excellent antioxidant and antibacterial properties of acylated polyphenols suggest that the investigated samples could also be beneficial to human health, requiring further analysis.

Phomopsis juniperivora. [Descriptions of Fungi and Bacteria].

1973 ◽  
Author(s):  
E. Punithalingam
Keyword(s):  
South Africa ◽  
New Zealand ◽  
Geographical Distribution ◽  
Pinus Banksiana ◽  
Taxus Baccata ◽  
Juniperus Ashei ◽  
Wide Range ◽  
Airborne Conidia ◽  
Chamaecyparis Lawsoniana ◽  
Thuja Orientalis

Abstract A description is provided for Phomopsis juniperivora. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On a wide range of conifers, including Juniperus ashei, J. horizontalis var. douglasii, J. japonica, J. scopulorum, J. chinensis, J. virginiana, J. communis, J. sabina var. tamariscifolia, Chamaecyparis lawsoniana, C. thyoides, Cupressus macrocarpa, C. arizonica, C. lusitanica, C. sempervirens, C. torulosa, Cryptomeriajaponica, Thuja orientalis, Larix decidua, Pinus banksiana, Pseudotsuga taxifolia, Cephalotaxus drupacea, Abies spp., Taxus baccata. GEOGRAPHICAL DISTRIBUTION: N. America (USA, Canada), Europe (UK, France, Denmark), Africa (Kenya, South Africa, Mozambique), Australasia and Oceania (New Zealand). TRANSMISSION: By airborne conidia produced in pycnidia on diseased tissues, liberated by a splash take-off mechanism.

scholarly journals Enrichment and Assessment of the Contributions of the Major Polyphenols to the Total Antioxidant Activity of Onion Extracts: A Fractionation by Flash Chromatography Approach

Antioxidants ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 175 ◽  
Author(s):  
Mohammad Hossain ◽  
Justine Lebelle ◽  
Rares Birsan ◽  
Dilip Rai
Keyword(s):  
Antioxidant Capacity ◽  
Flash Chromatography ◽  
Total Phenol Content ◽  
Antioxidant Power ◽  
Onion Extract ◽  
Antioxidant Capacities ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Quercetin Glycoside ◽  
Quercetin Aglycone

The present study extensively fractionated crude red onion extract in order to identify the polyphenols which contributed most in the total antioxidant capacity of the onion extract using a flash chromatography system. The flash separations produced 70 fractions which were tested for their total phenol content, total flavonoid content, and antioxidant capacities as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Out of these 70 fractions, four fractions which were representatives of the four major peaks of the flash chromatograms, were further analysed for their constituent polyphenols using liquid chromatography tandem mass spectrometry (LC-MS/MS). The main contributor of onion antioxidant capacity is quercetin glycoside followed by quercetin aglycone although quercetin aglycone had higher antioxidant capacity than its glycosidic counterparts. High abundance of quercetin glycosides such as quercetin-3,4′-diglucoside and quercetin-4′-glucoside had compensated for their relatively low antioxidant capacities. A Higher degree of glycosylation resulted in lower antioxidant capacity. The fractionation approach also contributed in enrichment of the onion antioxidant polyphenols. A >9 folds enrichment was possible by discarding the early fractions (fractions 1–15) which contained the main bulk of the extracts, predominantly sugars.

scholarly journals Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

2018 ◽  
Vol 20 (3) ◽  
pp. 541-550 ◽  
Keyword(s):  
Heavy Metal ◽  
Castanea Sativa ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

scholarly journals Comparative Nutrient Element and Antioxidant Characterization of Berry Fruit Species and Cultivars Grown in Hungary

HortScience ◽  
2008 ◽  
Vol 43 (6) ◽  
pp. 1711-1715 ◽  
Author(s):  
Attila Hegedűs ◽  
Emőke Balogh ◽  
Rita Engel ◽  
Béla Zoltan Sipos ◽  
János Papp ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Elemental Content ◽  
Total Phenol Content ◽  
Radical Scavenger ◽  
Black Currant ◽  
Antioxidant Power ◽  
Radical Scavenger Activity ◽  
Reducing Ability ◽  
Scavenger Activity

Fruits of four berry species (strawberry, raspberry, red and black currants) were compared in their elemental composition (Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, P, and Zn) and redox parameters involving total phenol content (TPC), ferric reducing ability (FRAP), 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and total radical scavenger activity (TRSA). Berry cultivars contained significantly higher (P ≤ 0.05) amounts of most detected elements as compared with apple with many maximum elemental values demonstrated by the black currant ‘Otelo’. Black currant also had the greatest antioxidant capacity as demonstrated through all conducted assays. The results obtained through FRAP, TPC, and TRSA assays were closely correlated, whereas TRSA and DPPH varied independently. Our study provides valuable information on the antioxidant capacity of several berry species grown in Hungary and highlights the crucial influence of cultivar on elemental content and antioxidant power of berry fruits. This study demonstrated the ability to select berry cultivars for specific nutritional purposes or assign parental lines in functional breeding programs.

scholarly journals Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

2023 ◽  
Vol 83 ◽  
Author(s):  
T. S. Neri ◽  
K. W. L. Silva ◽  
L. P. S. Maior ◽  
S. K. Oliveira-Silva ◽  
P. V. M. Azevedo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chemical Constituents ◽  
Total Phenolic ◽  
Plant Sample ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Dpph Method

Abstract Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.

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